UniProtKB - Q8NFH5 (NUP35_HUMAN)
Protein
Nucleoporin NUP35
Gene
NUP35
Organism
Homo sapiens (Human)
Status
Functioni
Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.1 Publication
GO - Molecular functioni
- identical protein binding Source: Ensembl
- phospholipid binding Source: GO_Central
- single-stranded DNA binding Source: GO_Central
- structural constituent of nuclear pore Source: GO_Central
GO - Biological processi
- cellular response to leukemia inhibitory factor Source: Ensembl
- intracellular transport of virus Source: Reactome
- mitotic nuclear envelope reassembly Source: Reactome
- mRNA export from nucleus Source: Reactome
- NLS-bearing protein import into nucleus Source: GO_Central
- nuclear pore organization Source: GO_Central
- protein sumoylation Source: Reactome
- regulation of cellular response to heat Source: Reactome
- regulation of gene silencing by miRNA Source: Reactome
- regulation of glycolytic process Source: Reactome
- regulation of transcription, DNA-templated Source: GO_Central
- tRNA export from nucleus Source: Reactome
- viral life cycle Source: Reactome
- viral process Source: Reactome
- viral transcription Source: Reactome
Keywordsi
Biological process | mRNA transport, Protein transport, Translocation, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q8NFH5 |
Reactomei | R-HSA-1169408, ISG15 antiviral mechanism R-HSA-159227, Transport of the SLBP independent Mature mRNA R-HSA-159230, Transport of the SLBP Dependant Mature mRNA R-HSA-159231, Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-165054, Rev-mediated nuclear export of HIV RNA R-HSA-168271, Transport of Ribonucleoproteins into the Host Nucleus R-HSA-168276, NS1 Mediated Effects on Host Pathways R-HSA-168325, Viral Messenger RNA Synthesis R-HSA-168333, NEP/NS2 Interacts with the Cellular Export Machinery R-HSA-170822, Regulation of Glucokinase by Glucokinase Regulatory Protein R-HSA-180746, Nuclear import of Rev protein R-HSA-180910, Vpr-mediated nuclear import of PICs R-HSA-191859, snRNP Assembly R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-3232142, SUMOylation of ubiquitinylation proteins R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-3371453, Regulation of HSF1-mediated heat shock response R-HSA-4085377, SUMOylation of SUMOylation proteins R-HSA-4551638, SUMOylation of chromatin organization proteins R-HSA-4570464, SUMOylation of RNA binding proteins R-HSA-4615885, SUMOylation of DNA replication proteins R-HSA-5578749, Transcriptional regulation by small RNAs R-HSA-5619107, Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) R-HSA-6784531, tRNA processing in the nucleus R-HSA-9609690, HCMV Early Events R-HSA-9610379, HCMV Late Events R-HSA-9615933, Postmitotic nuclear pore complex (NPC) reformation |
SIGNORi | Q8NFH5 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleoporin NUP35ImportedAlternative name(s): 35 kDa nucleoporin Mitotic phosphoprotein 44 Short name: MP-44 Nuclear pore complex protein Nup53 Nucleoporin NUP531 Publication |
Gene namesi | Name:NUP35 Synonyms:MP44, NUP53 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29797, NUP35 |
MIMi | 608140, gene |
neXtProti | NX_Q8NFH5 |
VEuPathDBi | HostDB:ENSG00000163002.12 |
Subcellular locationi
Nucleus
- nuclear pore complex 1 Publication
- Nucleus membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Tightly associated with the nuclear membrane and lamina.1 Publication
Cytosol
- cytosol Source: Reactome
Nucleus
- nuclear envelope Source: Reactome
- nuclear lamina Source: Ensembl
- nuclear membrane Source: HPA
- nuclear pore central transport channel Source: GO_Central
- nuclear pore nuclear basket Source: GO_Central
- nucleoplasm Source: HPA
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- host cell Source: GOC
Keywords - Cellular componenti
Membrane, Nuclear pore complex, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 129401 |
OpenTargetsi | ENSG00000163002 |
PharmGKBi | PA134861481 |
Miscellaneous databases
Pharosi | Q8NFH5, Tbio |
Genetic variation databases
BioMutai | NUP35 |
DMDMi | 74730292 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000234294 | 1 – 326 | Nucleoporin NUP35Add BLAST | 326 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 53 | PhosphoserineCombined sources | 1 | |
Modified residuei | 55 | PhosphoserineCombined sources | 1 | |
Modified residuei | 66 | PhosphoserineCombined sources | 1 | |
Modified residuei | 73 | PhosphoserineCombined sources | 1 | |
Modified residuei | 99 | PhosphoserineCombined sources | 1 | |
Modified residuei | 100 | PhosphoserineCombined sources | 1 | |
Modified residuei | 106 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 121 | PhosphoserineCombined sources | 1 | |
Modified residuei | 129 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 138 | PhosphoserineCombined sources | 1 | |
Modified residuei | 252 | PhosphoserineBy similarity | 1 | |
Modified residuei | 259 | PhosphoserineBy similarity | 1 | |
Modified residuei | 265 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 273 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 275 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 279 | PhosphoserineCombined sources | 1 | |
Modified residuei | 280 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 284 | PhosphoserineCombined sources | 1 | |
Modified residuei | 308 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8NFH5 |
jPOSTi | Q8NFH5 |
MassIVEi | Q8NFH5 |
MaxQBi | Q8NFH5 |
PaxDbi | Q8NFH5 |
PeptideAtlasi | Q8NFH5 |
PRIDEi | Q8NFH5 |
ProteomicsDBi | 4760 5514 73307 [Q8NFH5-1] |
PTM databases
iPTMneti | Q8NFH5 |
PhosphoSitePlusi | Q8NFH5 |
Expressioni
Gene expression databases
Bgeei | ENSG00000163002, Expressed in oocyte and 211 other tissues |
ExpressionAtlasi | Q8NFH5, baseline and differential |
Genevisiblei | Q8NFH5, HS |
Organism-specific databases
HPAi | ENSG00000163002, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with TMEM48/NDC1.
Forms a complex with NUP93, NUP155, NUP205 and lamin B; the interaction with NUP93 is direct.
2 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- identical protein binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 126190, 110 interactors |
ComplexPortali | CPX-873, Nuclear pore complex |
DIPi | DIP-47308N |
IntActi | Q8NFH5, 39 interactors |
MINTi | Q8NFH5 |
STRINGi | 9606.ENSP00000295119 |
Miscellaneous databases
RNActi | Q8NFH5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8NFH5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 170 – 250 | RRM Nup35-typePROSITE-ProRule annotationAdd BLAST | 81 |
Sequence similaritiesi
Belongs to the Nup35 family.Curated
Phylogenomic databases
eggNOGi | KOG4285, Eukaryota |
GeneTreei | ENSGT00390000005923 |
HOGENOMi | CLU_056189_0_0_1 |
InParanoidi | Q8NFH5 |
OMAi | APQNGNW |
OrthoDBi | 1163548at2759 |
PhylomeDBi | Q8NFH5 |
TreeFami | TF325369 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR017389, Nucleoporin_NUP53 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR007846, RRM_NUP35_dom |
PANTHERi | PTHR21527, PTHR21527, 1 hit |
Pfami | View protein in Pfam PF05172, Nup35_RRM, 1 hit |
PIRSFi | PIRSF038119, Nucleoporin_NUP53, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS51472, RRM_NUP35, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NFH5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAFAVEPQG PALGSEPMML GSPTSPKPGV NAQFLPGFLM GDLPAPVTPQ
60 70 80 90 100
PRSISGPSVG VMEMRSPLLA GGSPPQPVVP AHKDKSGAPP VRSIYDDISS
110 120 130 140 150
PGLGSTPLTS RRQPNISVMQ SPLVGVTSTP GTGQSMFSPA SIGQPRKTTL
160 170 180 190 200
SPAQLDPFYT QGDSLTSEDH LDDSWVTVFG FPQASASYIL LQFAQYGNIL
210 220 230 240 250
KHVMSNTGNW MHIRYQSKLQ ARKALSKDGR IFGESIMIGV KPCIDKSVME
260 270 280 290 300
SSDRCALSSP SLAFTPPIKT LGTPTQPGST PRISTMRPLA TAYKASTSDY
310 320
QVISDRQTPK KDESLVSKAM EYMFGW
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9IYQ7 | C9IYQ7_HUMAN | Nucleoporin NUP35 | NUP35 | 115 | Annotation score: | ||
F8WCF5 | F8WCF5_HUMAN | Nucleoporin NUP35 | NUP35 | 136 | Annotation score: | ||
C9JWU7 | C9JWU7_HUMAN | Nucleoporin NUP35 | NUP35 | 53 | Annotation score: | ||
C9JIY9 | C9JIY9_HUMAN | Nucleoporin NUP35 | NUP35 | 76 | Annotation score: | ||
F8WEL4 | F8WEL4_HUMAN | Nucleoporin NUP35 | NUP35 | 152 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 313 | E → G in AAL86379 (Ref. 2) Curated | 1 | |
Sequence conflicti | 318 | K → Q in AAL86379 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056210 | 1 – 135 | Missing in isoform 3. 1 PublicationAdd BLAST | 135 | |
Alternative sequenceiVSP_056211 | 1 – 17 | Missing in isoform 2. 1 PublicationAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF514993 mRNA Translation: AAM76704.1 AF411516 mRNA Translation: AAL86379.1 AK298199 mRNA Translation: BAG60469.1 AK302350 mRNA Translation: BAG63676.1 AC064871 Genomic DNA Translation: AAY24198.1 AC079249 Genomic DNA Translation: AAX88896.1 BC047029 mRNA Translation: AAH47029.1 BC061698 mRNA Translation: AAH61698.1 |
CCDSi | CCDS2290.1 [Q8NFH5-1] CCDS74614.1 [Q8NFH5-2] |
RefSeqi | NP_001274513.1, NM_001287584.1 [Q8NFH5-2] NP_001274514.1, NM_001287585.1 NP_612142.2, NM_138285.4 [Q8NFH5-1] XP_006712317.1, XM_006712254.2 [Q8NFH5-2] XP_011508878.1, XM_011510576.2 [Q8NFH5-2] XP_011508879.1, XM_011510577.2 [Q8NFH5-2] |
Genome annotation databases
Ensembli | ENST00000295119; ENSP00000295119; ENSG00000163002 [Q8NFH5-1] ENST00000409798; ENSP00000387305; ENSG00000163002 [Q8NFH5-2] |
GeneIDi | 129401 |
KEGGi | hsa:129401 |
UCSCi | uc002upf.5, human [Q8NFH5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF514993 mRNA Translation: AAM76704.1 AF411516 mRNA Translation: AAL86379.1 AK298199 mRNA Translation: BAG60469.1 AK302350 mRNA Translation: BAG63676.1 AC064871 Genomic DNA Translation: AAY24198.1 AC079249 Genomic DNA Translation: AAX88896.1 BC047029 mRNA Translation: AAH47029.1 BC061698 mRNA Translation: AAH61698.1 |
CCDSi | CCDS2290.1 [Q8NFH5-1] CCDS74614.1 [Q8NFH5-2] |
RefSeqi | NP_001274513.1, NM_001287584.1 [Q8NFH5-2] NP_001274514.1, NM_001287585.1 NP_612142.2, NM_138285.4 [Q8NFH5-1] XP_006712317.1, XM_006712254.2 [Q8NFH5-2] XP_011508878.1, XM_011510576.2 [Q8NFH5-2] XP_011508879.1, XM_011510577.2 [Q8NFH5-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4LIR | X-ray | 2.46 | A/B | 151-266 | [»] | |
SMRi | Q8NFH5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 126190, 110 interactors |
ComplexPortali | CPX-873, Nuclear pore complex |
DIPi | DIP-47308N |
IntActi | Q8NFH5, 39 interactors |
MINTi | Q8NFH5 |
STRINGi | 9606.ENSP00000295119 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | Q8NFH5 |
PhosphoSitePlusi | Q8NFH5 |
Genetic variation databases
BioMutai | NUP35 |
DMDMi | 74730292 |
Proteomic databases
EPDi | Q8NFH5 |
jPOSTi | Q8NFH5 |
MassIVEi | Q8NFH5 |
MaxQBi | Q8NFH5 |
PaxDbi | Q8NFH5 |
PeptideAtlasi | Q8NFH5 |
PRIDEi | Q8NFH5 |
ProteomicsDBi | 4760 5514 73307 [Q8NFH5-1] |
Protocols and materials databases
Antibodypediai | 19771, 209 antibodies |
DNASUi | 129401 |
Genome annotation databases
Ensembli | ENST00000295119; ENSP00000295119; ENSG00000163002 [Q8NFH5-1] ENST00000409798; ENSP00000387305; ENSG00000163002 [Q8NFH5-2] |
GeneIDi | 129401 |
KEGGi | hsa:129401 |
UCSCi | uc002upf.5, human [Q8NFH5-1] |
Organism-specific databases
CTDi | 129401 |
DisGeNETi | 129401 |
GeneCardsi | NUP35 |
HGNCi | HGNC:29797, NUP35 |
HPAi | ENSG00000163002, Low tissue specificity |
MIMi | 608140, gene |
neXtProti | NX_Q8NFH5 |
OpenTargetsi | ENSG00000163002 |
PharmGKBi | PA134861481 |
VEuPathDBi | HostDB:ENSG00000163002.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4285, Eukaryota |
GeneTreei | ENSGT00390000005923 |
HOGENOMi | CLU_056189_0_0_1 |
InParanoidi | Q8NFH5 |
OMAi | APQNGNW |
OrthoDBi | 1163548at2759 |
PhylomeDBi | Q8NFH5 |
TreeFami | TF325369 |
Enzyme and pathway databases
PathwayCommonsi | Q8NFH5 |
Reactomei | R-HSA-1169408, ISG15 antiviral mechanism R-HSA-159227, Transport of the SLBP independent Mature mRNA R-HSA-159230, Transport of the SLBP Dependant Mature mRNA R-HSA-159231, Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-165054, Rev-mediated nuclear export of HIV RNA R-HSA-168271, Transport of Ribonucleoproteins into the Host Nucleus R-HSA-168276, NS1 Mediated Effects on Host Pathways R-HSA-168325, Viral Messenger RNA Synthesis R-HSA-168333, NEP/NS2 Interacts with the Cellular Export Machinery R-HSA-170822, Regulation of Glucokinase by Glucokinase Regulatory Protein R-HSA-180746, Nuclear import of Rev protein R-HSA-180910, Vpr-mediated nuclear import of PICs R-HSA-191859, snRNP Assembly R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-3232142, SUMOylation of ubiquitinylation proteins R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-3371453, Regulation of HSF1-mediated heat shock response R-HSA-4085377, SUMOylation of SUMOylation proteins R-HSA-4551638, SUMOylation of chromatin organization proteins R-HSA-4570464, SUMOylation of RNA binding proteins R-HSA-4615885, SUMOylation of DNA replication proteins R-HSA-5578749, Transcriptional regulation by small RNAs R-HSA-5619107, Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) R-HSA-6784531, tRNA processing in the nucleus R-HSA-9609690, HCMV Early Events R-HSA-9610379, HCMV Late Events R-HSA-9615933, Postmitotic nuclear pore complex (NPC) reformation |
SIGNORi | Q8NFH5 |
Miscellaneous databases
BioGRID-ORCSi | 129401, 346 hits in 1000 CRISPR screens |
ChiTaRSi | NUP35, human |
GeneWikii | NUP35 |
GenomeRNAii | 129401 |
Pharosi | Q8NFH5, Tbio |
PROi | PR:Q8NFH5 |
RNActi | Q8NFH5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163002, Expressed in oocyte and 211 other tissues |
ExpressionAtlasi | Q8NFH5, baseline and differential |
Genevisiblei | Q8NFH5, HS |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR017389, Nucleoporin_NUP53 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR007846, RRM_NUP35_dom |
PANTHERi | PTHR21527, PTHR21527, 1 hit |
Pfami | View protein in Pfam PF05172, Nup35_RRM, 1 hit |
PIRSFi | PIRSF038119, Nucleoporin_NUP53, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS51472, RRM_NUP35, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUP35_HUMAN | |
Accessioni | Q8NFH5Primary (citable) accession number: Q8NFH5 Secondary accession number(s): B4DP57 Q8TDJ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | October 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families