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Protein

Nucleoporin NUP35

Gene

NUP35

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
R-HSA-159227 Transport of the SLBP independent Mature mRNA
R-HSA-159230 Transport of the SLBP Dependant Mature mRNA
R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-165054 Rev-mediated nuclear export of HIV RNA
R-HSA-168271 Transport of Ribonucleoproteins into the Host Nucleus
R-HSA-168276 NS1 Mediated Effects on Host Pathways
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-168333 NEP/NS2 Interacts with the Cellular Export Machinery
R-HSA-170822 Regulation of Glucokinase by Glucokinase Regulatory Protein
R-HSA-180746 Nuclear import of Rev protein
R-HSA-180910 Vpr-mediated nuclear import of PICs
R-HSA-191859 snRNP Assembly
R-HSA-3108214 SUMOylation of DNA damage response and repair proteins
R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
R-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-4551638 SUMOylation of chromatin organization proteins
R-HSA-4570464 SUMOylation of RNA binding proteins
R-HSA-4615885 SUMOylation of DNA replication proteins
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-6784531 tRNA processing in the nucleus

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP35Imported
Alternative name(s):
35 kDa nucleoporin
Mitotic phosphoprotein 44
Short name:
MP-44
Nuclear pore complex protein Nup53
Nucleoporin NUP531 Publication
Gene namesi
Name:NUP35
Synonyms:MP44, NUP53
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000163002.12
HGNCiHGNC:29797 NUP35
MIMi608140 gene
neXtProtiNX_Q8NFH5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi129401
OpenTargetsiENSG00000163002
PharmGKBiPA134861481

Polymorphism and mutation databases

DMDMi74730292

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002342941 – 326Nucleoporin NUP35Add BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineCombined sources1
Modified residuei55PhosphoserineCombined sources1
Modified residuei66PhosphoserineCombined sources1
Modified residuei73PhosphoserineCombined sources1
Modified residuei99PhosphoserineCombined sources1
Modified residuei100PhosphoserineCombined sources1
Modified residuei106PhosphothreonineCombined sources1
Modified residuei121PhosphoserineCombined sources1
Modified residuei129PhosphothreonineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei252PhosphoserineBy similarity1
Modified residuei259PhosphoserineBy similarity1
Modified residuei265PhosphothreonineCombined sources1
Modified residuei273PhosphothreonineCombined sources1
Modified residuei275PhosphothreonineCombined sources1
Modified residuei279PhosphoserineCombined sources1
Modified residuei280PhosphothreonineCombined sources1
Modified residuei284PhosphoserineCombined sources1
Modified residuei308PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8NFH5
MaxQBiQ8NFH5
PaxDbiQ8NFH5
PeptideAtlasiQ8NFH5
PRIDEiQ8NFH5
ProteomicsDBi73307

PTM databases

iPTMnetiQ8NFH5
PhosphoSitePlusiQ8NFH5

Expressioni

Gene expression databases

BgeeiENSG00000163002
CleanExiHS_NUP35
ExpressionAtlasiQ8NFH5 baseline and differential
GenevisibleiQ8NFH5 HS

Organism-specific databases

HPAiHPA018401
HPA018410
HPA018441

Interactioni

Subunit structurei

Interacts with TMEM48/NDC1. Forms a complex with NUP93, NUP155, NUP205 and lamin B; the interaction with NUP93 is direct.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KCTD9Q7L2735EBI-9050429,EBI-4397613

Protein-protein interaction databases

BioGridi126190, 77 interactors
DIPiDIP-47308N
IntActiQ8NFH5, 22 interactors
MINTiQ8NFH5
STRINGi9606.ENSP00000295119

Structurei

Secondary structure

1326
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi172 – 174Combined sources3
Beta strandi175 – 179Combined sources5
Helixi183 – 185Combined sources3
Helixi186 – 193Combined sources8
Beta strandi199 – 204Combined sources6
Beta strandi206 – 217Combined sources12
Helixi218 – 225Combined sources8
Turni226 – 229Combined sources4
Beta strandi230 – 232Combined sources3
Turni233 – 235Combined sources3
Beta strandi236 – 242Combined sources7
Helixi246 – 249Combined sources4

3D structure databases

ProteinModelPortaliQ8NFH5
SMRiQ8NFH5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini170 – 250RRM Nup35-typePROSITE-ProRule annotationAdd BLAST81

Sequence similaritiesi

Belongs to the Nup35 family.Curated

Phylogenomic databases

eggNOGiKOG4285 Eukaryota
ENOG4111ARE LUCA
GeneTreeiENSGT00390000005923
HOGENOMiHOG000231921
HOVERGENiHBG060396
InParanoidiQ8NFH5
KOiK14313
OMAiAPQNGNW
OrthoDBiEOG091G0DAQ
PhylomeDBiQ8NFH5
TreeFamiTF325369

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR017389 Nucleoporin_NUP53
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR007846 RRM_NUP35_dom
PfamiView protein in Pfam
PF05172 Nup35_RRM, 1 hit
PIRSFiPIRSF038119 Nucleoporin_NUP53, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS51472 RRM_NUP35, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NFH5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAFAVEPQG PALGSEPMML GSPTSPKPGV NAQFLPGFLM GDLPAPVTPQ
60 70 80 90 100
PRSISGPSVG VMEMRSPLLA GGSPPQPVVP AHKDKSGAPP VRSIYDDISS
110 120 130 140 150
PGLGSTPLTS RRQPNISVMQ SPLVGVTSTP GTGQSMFSPA SIGQPRKTTL
160 170 180 190 200
SPAQLDPFYT QGDSLTSEDH LDDSWVTVFG FPQASASYIL LQFAQYGNIL
210 220 230 240 250
KHVMSNTGNW MHIRYQSKLQ ARKALSKDGR IFGESIMIGV KPCIDKSVME
260 270 280 290 300
SSDRCALSSP SLAFTPPIKT LGTPTQPGST PRISTMRPLA TAYKASTSDY
310 320
QVISDRQTPK KDESLVSKAM EYMFGW
Length:326
Mass (Da):34,774
Last modified:October 1, 2002 - v1
Checksum:i6420B0EF1AE31013
GO
Isoform 2 (identifier: Q8NFH5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: No experimental confirmation available.
Show »
Length:309
Mass (Da):33,120
Checksum:iBA43C9E8B0BD3BD1
GO
Isoform 3 (identifier: Q8NFH5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-135: Missing.

Note: No experimental confirmation available.
Show »
Length:191
Mass (Da):21,142
Checksum:i96265A68DC195F33
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti313E → G in AAL86379 (Ref. 2) Curated1
Sequence conflicti318K → Q in AAL86379 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0562101 – 135Missing in isoform 3. 1 PublicationAdd BLAST135
Alternative sequenceiVSP_0562111 – 17Missing in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF514993 mRNA Translation: AAM76704.1
AF411516 mRNA Translation: AAL86379.1
AK298199 mRNA Translation: BAG60469.1
AK302350 mRNA Translation: BAG63676.1
AC064871 Genomic DNA Translation: AAY24198.1
AC079249 Genomic DNA Translation: AAX88896.1
BC047029 mRNA Translation: AAH47029.1
BC061698 mRNA Translation: AAH61698.1
CCDSiCCDS2290.1 [Q8NFH5-1]
CCDS74614.1 [Q8NFH5-2]
RefSeqiNP_001274513.1, NM_001287584.1 [Q8NFH5-2]
NP_001274514.1, NM_001287585.1
NP_612142.2, NM_138285.4 [Q8NFH5-1]
XP_006712317.1, XM_006712254.2 [Q8NFH5-2]
XP_011508878.1, XM_011510576.2 [Q8NFH5-2]
XP_011508879.1, XM_011510577.2 [Q8NFH5-2]
UniGeneiHs.180591

Genome annotation databases

EnsembliENST00000295119; ENSP00000295119; ENSG00000163002 [Q8NFH5-1]
ENST00000409798; ENSP00000387305; ENSG00000163002 [Q8NFH5-2]
GeneIDi129401
KEGGihsa:129401
UCSCiuc002upf.5 human [Q8NFH5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNUP35_HUMAN
AccessioniPrimary (citable) accession number: Q8NFH5
Secondary accession number(s): B4DP57
, B4DYB4, Q4ZFZ9, Q53S95, Q8TDJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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