UniProtKB - Q8NFH3 (NUP43_HUMAN)
Protein
Nucleoporin Nup43
Gene
NUP43
Organism
Homo sapiens (Human)
Status
Functioni
Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation.1 Publication
GO - Biological processi
- cell division Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB-KW
- intracellular transport of virus Source: Reactome
- mitotic nuclear envelope reassembly Source: Reactome
- mitotic spindle organization Source: Reactome
- mRNA export from nucleus Source: Reactome
- protein sumoylation Source: Reactome
- regulation of cellular response to heat Source: Reactome
- regulation of gene silencing by miRNA Source: Reactome
- regulation of glycolytic process Source: Reactome
- tRNA export from nucleus Source: Reactome
- viral life cycle Source: Reactome
- viral process Source: Reactome
- viral transcription Source: Reactome
Keywordsi
Biological process | Cell cycle, Cell division, Chromosome partition, Mitosis, mRNA transport, Protein transport, Translocation, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q8NFH3 |
Reactomei | R-HSA-1169408, ISG15 antiviral mechanism R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-159227, Transport of the SLBP independent Mature mRNA R-HSA-159230, Transport of the SLBP Dependant Mature mRNA R-HSA-159231, Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-165054, Rev-mediated nuclear export of HIV RNA R-HSA-168271, Transport of Ribonucleoproteins into the Host Nucleus R-HSA-168276, NS1 Mediated Effects on Host Pathways R-HSA-168325, Viral Messenger RNA Synthesis R-HSA-168333, NEP/NS2 Interacts with the Cellular Export Machinery R-HSA-170822, Regulation of Glucokinase by Glucokinase Regulatory Protein R-HSA-180746, Nuclear import of Rev protein R-HSA-180910, Vpr-mediated nuclear import of PICs R-HSA-191859, snRNP Assembly R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-3232142, SUMOylation of ubiquitinylation proteins R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-3371453, Regulation of HSF1-mediated heat shock response R-HSA-4085377, SUMOylation of SUMOylation proteins R-HSA-4551638, SUMOylation of chromatin organization proteins R-HSA-4570464, SUMOylation of RNA binding proteins R-HSA-4615885, SUMOylation of DNA replication proteins R-HSA-5578749, Transcriptional regulation by small RNAs R-HSA-5619107, Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) R-HSA-5663220, RHO GTPases Activate Formins R-HSA-6784531, tRNA processing in the nucleus R-HSA-68877, Mitotic Prometaphase R-HSA-9609690, HCMV Early Events R-HSA-9610379, HCMV Late Events R-HSA-9615933, Postmitotic nuclear pore complex (NPC) reformation R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SignaLinki | Q8NFH3 |
SIGNORi | Q8NFH3 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleoporin Nup43Alternative name(s): Nup107-160 subcomplex subunit Nup43 p42 |
Gene namesi | Name:NUP43 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:21182, NUP43 |
MIMi | 608141, gene |
neXtProti | NX_Q8NFH3 |
VEuPathDBi | HostDB:ENSG00000120253.13 |
Subcellular locationi
Nucleus
Other locations
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear envelope Source: Reactome
- nuclear pore outer ring Source: UniProtKB
- nuclear speck Source: HPA
- nucleoplasm Source: HPA
Other locations
- condensed chromosome kinetochore Source: UniProtKB-SubCell
- host cell Source: GOC
Keywords - Cellular componenti
Centromere, Chromosome, Kinetochore, Nuclear pore complex, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 348995 |
OpenTargetsi | ENSG00000120253 |
PharmGKBi | PA134930788 |
Miscellaneous databases
Pharosi | Q8NFH3, Tdark |
Genetic variation databases
BioMutai | NUP43 |
DMDMi | 27923819 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051111 | 1 – 380 | Nucleoporin Nup43Add BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q8NFH3 |
jPOSTi | Q8NFH3 |
MassIVEi | Q8NFH3 |
MaxQBi | Q8NFH3 |
PaxDbi | Q8NFH3 |
PeptideAtlasi | Q8NFH3 |
PRIDEi | Q8NFH3 |
ProteomicsDBi | 5814 73305 [Q8NFH3-1] |
PTM databases
iPTMneti | Q8NFH3 |
PhosphoSitePlusi | Q8NFH3 |
SwissPalmi | Q8NFH3 |
Expressioni
Gene expression databases
Bgeei | ENSG00000120253, Expressed in oocyte and 249 other tissues |
ExpressionAtlasi | Q8NFH3, baseline and differential |
Genevisiblei | Q8NFH3, HS |
Organism-specific databases
HPAi | ENSG00000120253, Low tissue specificity |
Interactioni
Subunit structurei
Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex includes NUP160, NUP133, NUP107, NUP98, NUP85, NUP43, NUP37, SEH1 and SEC13.
2 PublicationsBinary interactionsi
Hide detailsQ8NFH3
Protein-protein interaction databases
BioGRIDi | 131544, 91 interactors |
ComplexPortali | CPX-873, Nuclear pore complex |
CORUMi | Q8NFH3 |
IntActi | Q8NFH3, 47 interactors |
MINTi | Q8NFH3 |
STRINGi | 9606.ENSP00000342262 |
Miscellaneous databases
RNActi | Q8NFH3, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8NFH3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 8 – 57 | WD 1Add BLAST | 50 | |
Repeati | 72 – 110 | WD 2Add BLAST | 39 | |
Repeati | 127 – 166 | WD 3Add BLAST | 40 | |
Repeati | 170 – 208 | WD 4Add BLAST | 39 | |
Repeati | 215 – 255 | WD 5Add BLAST | 41 | |
Repeati | 259 – 299 | WD 6Add BLAST | 41 |
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG4714, Eukaryota |
GeneTreei | ENSGT00390000004803 |
HOGENOMi | CLU_060663_1_0_1 |
InParanoidi | Q8NFH3 |
OMAi | FRHHQNN |
PhylomeDBi | Q8NFH3 |
TreeFami | TF321692 |
Family and domain databases
InterProi | View protein in InterPro IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 5 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 2 hits PS50294, WD_REPEATS_REGION, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NFH3-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEEIYAKFVS QKISKTRWRP LPPGSLQTAE TFATGSWDNE ENYISLWSIG
60 70 80 90 100
DFGNLDSDGG FEGDHQLLCD IRHHGDVMDL QFFDQERIVA ASSTGCVTVF
110 120 130 140 150
LHHPNNQTLS VNQQWTTAHY HTGPGSPSYS SAPCTGVVCN NPEIVTVGED
160 170 180 190 200
GRINLFRADH KEAVRTIDNA DSSTLHAVTF LRTPEILTVN SIGQLKIWDF
210 220 230 240 250
RQQGNEPSQI LSLTGDRVPL HCVDRHPNQQ HVVATGGQDG MLSIWDVRQG
260 270 280 290 300
TMPVSLLKAH EAEMWEVHFH PSNPEHLFTC SEDGSLWHWD ASTDVPEKSS
310 320 330 340 350
LFHQGGRSST FLSHSISNQA NVHQSVISSW LSTDPAKDRI EITSLLPSRS
360 370 380
LSVNTLDVLG PCLVCGTDAE AIYVTRHLFS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5H0E3 | F5H0E3_HUMAN | Nucleoporin Nup43 | NUP43 | 178 | Annotation score: | ||
B4DVD1 | B4DVD1_HUMAN | Nucleoporin Nup43 | NUP43 | 187 | Annotation score: | ||
B4E301 | B4E301_HUMAN | Nucleoporin Nup43 | NUP43 | 117 | Annotation score: |
Sequence cautioni
The sequence BAB14536 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 247 | V → A in BAB14536 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056167 | 168 – 264 | DNADS…HEAEM → V in isoform 2. 1 PublicationAdd BLAST | 97 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF514997 mRNA Translation: AAM76708.1 AK023349 mRNA Translation: BAB14536.1 Different initiation. AK304244 mRNA Translation: BAG65112.1 AL355312 Genomic DNA No translation available. BC065028 mRNA Translation: AAH65028.1 |
CCDSi | CCDS5218.1 [Q8NFH3-1] |
RefSeqi | NP_942590.1, NM_198887.2 [Q8NFH3-1] |
Genome annotation databases
Ensembli | ENST00000340413; ENSP00000342262; ENSG00000120253 [Q8NFH3-1] ENST00000367404; ENSP00000356374; ENSG00000120253 [Q8NFH3-2] |
GeneIDi | 348995 |
KEGGi | hsa:348995 |
UCSCi | uc003qmz.5, human [Q8NFH3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF514997 mRNA Translation: AAM76708.1 AK023349 mRNA Translation: BAB14536.1 Different initiation. AK304244 mRNA Translation: BAG65112.1 AL355312 Genomic DNA No translation available. BC065028 mRNA Translation: AAH65028.1 |
CCDSi | CCDS5218.1 [Q8NFH3-1] |
RefSeqi | NP_942590.1, NM_198887.2 [Q8NFH3-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4I79 | X-ray | 1.75 | A/B | 1-380 | [»] | |
5A9Q | electron microscopy | 23.00 | 0/9/I/R | 1-380 | [»] | |
SMRi | Q8NFH3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 131544, 91 interactors |
ComplexPortali | CPX-873, Nuclear pore complex |
CORUMi | Q8NFH3 |
IntActi | Q8NFH3, 47 interactors |
MINTi | Q8NFH3 |
STRINGi | 9606.ENSP00000342262 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | Q8NFH3 |
PhosphoSitePlusi | Q8NFH3 |
SwissPalmi | Q8NFH3 |
Genetic variation databases
BioMutai | NUP43 |
DMDMi | 27923819 |
Proteomic databases
EPDi | Q8NFH3 |
jPOSTi | Q8NFH3 |
MassIVEi | Q8NFH3 |
MaxQBi | Q8NFH3 |
PaxDbi | Q8NFH3 |
PeptideAtlasi | Q8NFH3 |
PRIDEi | Q8NFH3 |
ProteomicsDBi | 5814 73305 [Q8NFH3-1] |
Protocols and materials databases
Antibodypediai | 33283, 224 antibodies |
DNASUi | 348995 |
Genome annotation databases
Ensembli | ENST00000340413; ENSP00000342262; ENSG00000120253 [Q8NFH3-1] ENST00000367404; ENSP00000356374; ENSG00000120253 [Q8NFH3-2] |
GeneIDi | 348995 |
KEGGi | hsa:348995 |
UCSCi | uc003qmz.5, human [Q8NFH3-1] |
Organism-specific databases
CTDi | 348995 |
DisGeNETi | 348995 |
GeneCardsi | NUP43 |
HGNCi | HGNC:21182, NUP43 |
HPAi | ENSG00000120253, Low tissue specificity |
MIMi | 608141, gene |
neXtProti | NX_Q8NFH3 |
OpenTargetsi | ENSG00000120253 |
PharmGKBi | PA134930788 |
VEuPathDBi | HostDB:ENSG00000120253.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4714, Eukaryota |
GeneTreei | ENSGT00390000004803 |
HOGENOMi | CLU_060663_1_0_1 |
InParanoidi | Q8NFH3 |
OMAi | FRHHQNN |
PhylomeDBi | Q8NFH3 |
TreeFami | TF321692 |
Enzyme and pathway databases
PathwayCommonsi | Q8NFH3 |
Reactomei | R-HSA-1169408, ISG15 antiviral mechanism R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-159227, Transport of the SLBP independent Mature mRNA R-HSA-159230, Transport of the SLBP Dependant Mature mRNA R-HSA-159231, Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-165054, Rev-mediated nuclear export of HIV RNA R-HSA-168271, Transport of Ribonucleoproteins into the Host Nucleus R-HSA-168276, NS1 Mediated Effects on Host Pathways R-HSA-168325, Viral Messenger RNA Synthesis R-HSA-168333, NEP/NS2 Interacts with the Cellular Export Machinery R-HSA-170822, Regulation of Glucokinase by Glucokinase Regulatory Protein R-HSA-180746, Nuclear import of Rev protein R-HSA-180910, Vpr-mediated nuclear import of PICs R-HSA-191859, snRNP Assembly R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-3108214, SUMOylation of DNA damage response and repair proteins R-HSA-3232142, SUMOylation of ubiquitinylation proteins R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-3371453, Regulation of HSF1-mediated heat shock response R-HSA-4085377, SUMOylation of SUMOylation proteins R-HSA-4551638, SUMOylation of chromatin organization proteins R-HSA-4570464, SUMOylation of RNA binding proteins R-HSA-4615885, SUMOylation of DNA replication proteins R-HSA-5578749, Transcriptional regulation by small RNAs R-HSA-5619107, Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) R-HSA-5663220, RHO GTPases Activate Formins R-HSA-6784531, tRNA processing in the nucleus R-HSA-68877, Mitotic Prometaphase R-HSA-9609690, HCMV Early Events R-HSA-9610379, HCMV Late Events R-HSA-9615933, Postmitotic nuclear pore complex (NPC) reformation R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SignaLinki | Q8NFH3 |
SIGNORi | Q8NFH3 |
Miscellaneous databases
BioGRID-ORCSi | 348995, 637 hits in 1011 CRISPR screens |
ChiTaRSi | NUP43, human |
GeneWikii | NUP43 |
GenomeRNAii | 348995 |
Pharosi | Q8NFH3, Tdark |
PROi | PR:Q8NFH3 |
RNActi | Q8NFH3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000120253, Expressed in oocyte and 249 other tissues |
ExpressionAtlasi | Q8NFH3, baseline and differential |
Genevisiblei | Q8NFH3, HS |
Family and domain databases
InterProi | View protein in InterPro IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 5 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 2 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUP43_HUMAN | |
Accessioni | Q8NFH3Primary (citable) accession number: Q8NFH3 Secondary accession number(s): B4E2F0, Q9H8S0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 27, 2003 |
Last sequence update: | October 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references