UniProtKB - Q8NFA0 (UBP32_HUMAN)
Protein
Ubiquitin carboxyl-terminal hydrolase 32
Gene
USP32
Organism
Homo sapiens (Human)
Status
Functioni
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 743 | NucleophilePROSITE-ProRule annotation | 1 | |
Active sitei | 1526 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 241 – 252 | 1PROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 277 – 288 | 2PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- cysteine-type peptidase activity Source: UniProtKB-KW
- thiol-dependent ubiquitin-specific protease activity Source: UniProtKB
GO - Biological processi
- protein deubiquitination Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Ubl conjugation pathway |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q8NFA0 |
Protein family/group databases
MEROPSi | C19.044 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin carboxyl-terminal hydrolase 32 (EC:3.4.19.12)Alternative name(s): Deubiquitinating enzyme 32 Renal carcinoma antigen NY-REN-60 Ubiquitin thioesterase 32 Ubiquitin-specific-processing protease 32 |
Gene namesi | Name:USP32 Synonyms:USP10 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19143, USP32 |
MIMi | 607740, gene |
neXtProti | NX_Q8NFA0 |
VEuPathDBi | HostDB:ENSG00000170832.12 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus 1 Publication
Other locations
- Membrane Curated; Lipid-anchor Curated
Cytosol
- cytosol Source: HPA
Golgi apparatus
- Golgi apparatus Source: UniProtKB
Other locations
- membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 84669 |
OpenTargetsi | ENSG00000170832 |
PharmGKBi | PA134982542 |
Miscellaneous databases
Pharosi | Q8NFA0, Tbio |
Genetic variation databases
BioMutai | USP32 |
DMDMi | 47606649 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080663 | 1 – 1601 | Ubiquitin carboxyl-terminal hydrolase 32Add BLAST | 1601 | |
PropeptideiPRO_0000396658 | 1602 – 1604 | Removed in mature formSequence analysis | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1173 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 1350 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1372 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1376 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1454 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1588 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1601 | Cysteine methyl esterSequence analysis | 1 | |
Lipidationi | 1601 | S-farnesyl cysteineSequence analysis | 1 |
Keywords - PTMi
Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
EPDi | Q8NFA0 |
jPOSTi | Q8NFA0 |
MassIVEi | Q8NFA0 |
MaxQBi | Q8NFA0 |
PaxDbi | Q8NFA0 |
PeptideAtlasi | Q8NFA0 |
PRIDEi | Q8NFA0 |
ProteomicsDBi | 69353 73280 [Q8NFA0-1] |
PTM databases
iPTMneti | Q8NFA0 |
PhosphoSitePlusi | Q8NFA0 |
SwissPalmi | Q8NFA0 |
Expressioni
Gene expression databases
Bgeei | ENSG00000170832, Expressed in corpus callosum and 230 other tissues |
ExpressionAtlasi | Q8NFA0, baseline and differential |
Genevisiblei | Q8NFA0, HS |
Organism-specific databases
HPAi | ENSG00000170832, Tissue enhanced (testis) |
Interactioni
Binary interactionsi
Hide detailsQ8NFA0
With | #Exp. | IntAct |
---|---|---|
ZDHHC17 [Q8IUH5] | 2 | EBI-2511075,EBI-524753 |
Isoform 2 [Q8NFA0-2]
Protein-protein interaction databases
BioGRIDi | 124189, 66 interactors |
DIPi | DIP-53613N |
IntActi | Q8NFA0, 47 interactors |
MINTi | Q8NFA0 |
STRINGi | 9606.ENSP00000300896 |
Miscellaneous databases
RNActi | Q8NFA0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 91 – 126 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 228 – 263 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 264 – 299 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 369 – 585 | DUSPPROSITE-ProRule annotationAdd BLAST | 217 | |
Domaini | 734 – 1567 | USPAdd BLAST | 834 |
Sequence similaritiesi
Belongs to the peptidase C19 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0044, Eukaryota KOG1870, Eukaryota |
GeneTreei | ENSGT00940000155797 |
HOGENOMi | CLU_061823_0_0_1 |
InParanoidi | Q8NFA0 |
OMAi | FMLWNVQ |
OrthoDBi | 1283205at2759 |
PhylomeDBi | Q8NFA0 |
TreeFami | TF324190 |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
InterProi | View protein in InterPro IPR035927, DUSP-like_sf IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR006615, Pept_C19_DUSP IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF06337, DUSP, 1 hit PF13202, EF-hand_5, 2 hits PF00443, UCH, 1 hit |
SMARTi | View protein in SMART SM00695, DUSP, 1 hit SM00054, EFh, 2 hits |
SUPFAMi | SSF143791, SSF143791, 1 hit SSF47473, SSF47473, 2 hits SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS51283, DUSP, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 3 hits PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NFA0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGAKESRIGF LSYEEALRRV TDVELKRLKD AFKRTCGLSY YMGQHCFIRE
60 70 80 90 100
VLGDGVPPKV AEVIYCSFGG TSKGLHFNNL IVGLVLLTRG KDEEKAKYIF
110 120 130 140 150
SLFSSESGNY VIREEMERML HVVDGKVPDT LRKCFSEGEK VNYEKFRNWL
160 170 180 190 200
FLNKDAFTFS RWLLSGGVYV TLTDDSDTPT FYQTLAGVTH LEESDIIDLE
210 220 230 240 250
KRYWLLKAQS RTGRFDLETF GPLVSPPIRP SLSEGLFNAF DENRDNHIDF
260 270 280 290 300
KEISCGLSAC CRGPLAERQK FCFKVFDVDR DGVLSRVELR DMVVALLEVW
310 320 330 340 350
KDNRTDDIPE LHMDLSDIVE GILNAHDTTK MGHLTLEDYQ IWSVKNVLAN
360 370 380 390 400
EFLNLLFQVC HIVLGLRPAT PEEEGQIIRG WLERESRYGL QAGHNWFIIS
410 420 430 440 450
MQWWQQWKEY VKYDANPVVI EPSSVLNGGK YSFGTAAHPM EQVEDRIGSS
460 470 480 490 500
LSYVNTTEEK FSDNISTASE ASETAGSGFL YSATPGADVC FARQHNTSDN
510 520 530 540 550
NNQCLLGANG NILLHLNPQK PGAIDNQPLV TQEPVKATSL TLEGGRLKRT
560 570 580 590 600
PQLIHGRDYE MVPEPVWRAL YHWYGANLAL PRPVIKNSKT DIPELELFPR
610 620 630 640 650
YLLFLRQQPA TRTQQSNIWV NMGNVPSPNA PLKRVLAYTG CFSRMQTIKE
660 670 680 690 700
IHEYLSQRLR IKEEDMRLWL YNSENYLTLL DDEDHKLEYL KIQDEQHLVI
710 720 730 740 750
EVRNKDMSWP EEMSFIANSS KIDRHKVPTE KGATGLSNLG NTCFMNSSIQ
760 770 780 790 800
CVSNTQPLTQ YFISGRHLYE LNRTNPIGMK GHMAKCYGDL VQELWSGTQK
810 820 830 840 850
NVAPLKLRWT IAKYAPRFNG FQQQDSQELL AFLLDGLHED LNRVHEKPYV
860 870 880 890 900
ELKDSDGRPD WEVAAEAWDN HLRRNRSIVV DLFHGQLRSQ VKCKTCGHIS
910 920 930 940 950
VRFDPFNFLS LPLPMDSYMH LEITVIKLDG TTPVRYGLRL NMDEKYTGLK
960 970 980 990 1000
KQLSDLCGLN SEQILLAEVH GSNIKNFPQD NQKVRLSVSG FLCAFEIPVP
1010 1020 1030 1040 1050
VSPISASSPT QTDFSSSPST NEMFTLTTNG DLPRPIFIPN GMPNTVVPCG
1060 1070 1080 1090 1100
TEKNFTNGMV NGHMPSLPDS PFTGYIIAVH RKMMRTELYF LSSQKNRPSL
1110 1120 1130 1140 1150
FGMPLIVPCT VHTRKKDLYD AVWIQVSRLA SPLPPQEASN HAQDCDDSMG
1160 1170 1180 1190 1200
YQYPFTLRVV QKDGNSCAWC PWYRFCRGCK IDCGEDRAFI GNAYIAVDWD
1210 1220 1230 1240 1250
PTALHLRYQT SQERVVDEHE SVEQSRRAQA EPINLDSCLR AFTSEEELGE
1260 1270 1280 1290 1300
NEMYYCSKCK THCLATKKLD LWRLPPILII HLKRFQFVNG RWIKSQKIVK
1310 1320 1330 1340 1350
FPRESFDPSA FLVPRDPALC QHKPLTPQGD ELSEPRILAR EVKKVDAQSS
1360 1370 1380 1390 1400
AGEEDVLLSK SPSSLSANII SSPKGSPSSS RKSGTSCPSS KNSSPNSSPR
1410 1420 1430 1440 1450
TLGRSKGRLR LPQIGSKNKL SSSKENLDAS KENGAGQICE LADALSRGHV
1460 1470 1480 1490 1500
LGGSQPELVT PQDHEVALAN GFLYEHEACG NGYSNGQLGN HSEEDSTDDQ
1510 1520 1530 1540 1550
REDTRIKPIY NLYAISCHSG ILGGGHYVTY AKNPNCKWYC YNDSSCKELH
1560 1570 1580 1590 1600
PDEIDTDSAY ILFYEQQGID YAQFLPKTDG KKMADTSSMD EDFESDYKKY
CVLQ
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EKZ1 | K7EKZ1_HUMAN | Ubiquitinyl hydrolase 1 | USP32 | 847 | Annotation score: | ||
K7EQL6 | K7EQL6_HUMAN | Ubiquitinyl hydrolase 1 | USP32 | 1,274 | Annotation score: | ||
K7EN13 | K7EN13_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP32 | 174 | Annotation score: | ||
K7EII4 | K7EII4_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP32 | 246 | Annotation score: | ||
K7EKK3 | K7EKK3_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP32 | 133 | Annotation score: | ||
K7ENU6 | K7ENU6_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP32 | 153 | Annotation score: | ||
K7EPL4 | K7EPL4_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP32 | 87 | Annotation score: | ||
K7EL53 | K7EL53_HUMAN | Ubiquitin carboxyl-terminal hydrola... | USP32 | 87 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 884 | H → R in AAK30207 (Ref. 2) Curated | 1 | |
Sequence conflicti | 1167 | C → S in AAK30207 (Ref. 2) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051536 | 76 | H → Y. Corresponds to variant dbSNP:rs7208980Ensembl. | 1 | |
Natural variantiVAR_051537 | 1469 | A → G. Corresponds to variant dbSNP:rs3207630Ensembl. | 1 | |
Natural variantiVAR_051538 | 1568 | G → R. Corresponds to variant dbSNP:rs1053621Ensembl. | 1 | |
Natural variantiVAR_051539 | 1578 | T → I. Corresponds to variant dbSNP:rs1053625Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056307 | 380 – 390 | GWLERESRYGL → TLETDQIYTRN in isoform 2. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_056308 | 391 – 1604 | Missing in isoform 2. 1 PublicationAdd BLAST | 1214 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF533230 mRNA Translation: AAM97922.1 AF350251 mRNA Translation: AAK30207.1 AC003962 Genomic DNA No translation available. AC025048 Genomic DNA No translation available. AC037475 Genomic DNA No translation available. AC104763 Genomic DNA No translation available. BC054344 mRNA Translation: AAH54344.1 AF155116 mRNA Translation: AAD42882.1 |
CCDSi | CCDS32697.1 [Q8NFA0-1] |
RefSeqi | NP_115971.2, NM_032582.3 [Q8NFA0-1] |
Genome annotation databases
Ensembli | ENST00000300896; ENSP00000300896; ENSG00000170832 [Q8NFA0-1] ENST00000393003; ENSP00000376727; ENSG00000170832 [Q8NFA0-2] |
GeneIDi | 84669 |
KEGGi | hsa:84669 |
UCSCi | uc002iyo.2, human [Q8NFA0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF533230 mRNA Translation: AAM97922.1 AF350251 mRNA Translation: AAK30207.1 AC003962 Genomic DNA No translation available. AC025048 Genomic DNA No translation available. AC037475 Genomic DNA No translation available. AC104763 Genomic DNA No translation available. BC054344 mRNA Translation: AAH54344.1 AF155116 mRNA Translation: AAD42882.1 |
CCDSi | CCDS32697.1 [Q8NFA0-1] |
RefSeqi | NP_115971.2, NM_032582.3 [Q8NFA0-1] |
3D structure databases
SMRi | Q8NFA0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 124189, 66 interactors |
DIPi | DIP-53613N |
IntActi | Q8NFA0, 47 interactors |
MINTi | Q8NFA0 |
STRINGi | 9606.ENSP00000300896 |
Protein family/group databases
MEROPSi | C19.044 |
PTM databases
iPTMneti | Q8NFA0 |
PhosphoSitePlusi | Q8NFA0 |
SwissPalmi | Q8NFA0 |
Genetic variation databases
BioMutai | USP32 |
DMDMi | 47606649 |
Proteomic databases
EPDi | Q8NFA0 |
jPOSTi | Q8NFA0 |
MassIVEi | Q8NFA0 |
MaxQBi | Q8NFA0 |
PaxDbi | Q8NFA0 |
PeptideAtlasi | Q8NFA0 |
PRIDEi | Q8NFA0 |
ProteomicsDBi | 69353 73280 [Q8NFA0-1] |
Protocols and materials databases
Antibodypediai | 31170, 75 antibodies |
DNASUi | 84669 |
Genome annotation databases
Ensembli | ENST00000300896; ENSP00000300896; ENSG00000170832 [Q8NFA0-1] ENST00000393003; ENSP00000376727; ENSG00000170832 [Q8NFA0-2] |
GeneIDi | 84669 |
KEGGi | hsa:84669 |
UCSCi | uc002iyo.2, human [Q8NFA0-1] |
Organism-specific databases
CTDi | 84669 |
DisGeNETi | 84669 |
GeneCardsi | USP32 |
HGNCi | HGNC:19143, USP32 |
HPAi | ENSG00000170832, Tissue enhanced (testis) |
MIMi | 607740, gene |
neXtProti | NX_Q8NFA0 |
OpenTargetsi | ENSG00000170832 |
PharmGKBi | PA134982542 |
VEuPathDBi | HostDB:ENSG00000170832.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0044, Eukaryota KOG1870, Eukaryota |
GeneTreei | ENSGT00940000155797 |
HOGENOMi | CLU_061823_0_0_1 |
InParanoidi | Q8NFA0 |
OMAi | FMLWNVQ |
OrthoDBi | 1283205at2759 |
PhylomeDBi | Q8NFA0 |
TreeFami | TF324190 |
Enzyme and pathway databases
PathwayCommonsi | Q8NFA0 |
Miscellaneous databases
BioGRID-ORCSi | 84669, 34 hits in 998 CRISPR screens |
ChiTaRSi | USP32, human |
GenomeRNAii | 84669 |
Pharosi | Q8NFA0, Tbio |
PROi | PR:Q8NFA0 |
RNActi | Q8NFA0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000170832, Expressed in corpus callosum and 230 other tissues |
ExpressionAtlasi | Q8NFA0, baseline and differential |
Genevisiblei | Q8NFA0, HS |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
InterProi | View protein in InterPro IPR035927, DUSP-like_sf IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR006615, Pept_C19_DUSP IPR001394, Peptidase_C19_UCH IPR018200, USP_CS IPR028889, USP_dom |
Pfami | View protein in Pfam PF06337, DUSP, 1 hit PF13202, EF-hand_5, 2 hits PF00443, UCH, 1 hit |
SMARTi | View protein in SMART SM00695, DUSP, 1 hit SM00054, EFh, 2 hits |
SUPFAMi | SSF143791, SSF143791, 1 hit SSF47473, SSF47473, 2 hits SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS51283, DUSP, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 3 hits PS00972, USP_1, 1 hit PS00973, USP_2, 1 hit PS50235, USP_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBP32_HUMAN | |
Accessioni | Q8NFA0Primary (citable) accession number: Q8NFA0 Secondary accession number(s): Q7Z5T3, Q9BX85, Q9Y591 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2003 |
Last sequence update: | October 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 176 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families