UniProtKB - Q8NF86 (PRS33_HUMAN)
Protein
Serine protease 33
Gene
PRSS33
Organism
Homo sapiens (Human)
Status
Functioni
Serine protease that has amidolytic activity, cleaving its substrates before Arg residues.1 Publication
Kineticsi
- KM=6.9 mM for H-DL-Val-Leu-Arg-pNA1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 77 | Charge relay systemBy similarity | 1 | |
Active sitei | 126 | Charge relay systemBy similarity | 1 | |
Active sitei | 231 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: UniProtKB
GO - Biological processi
- protein kinase C signaling Source: UniProtKB
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
PathwayCommonsi | Q8NF86 |
Protein family/group databases
MEROPSi | S01.075 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PRSS33 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30405, PRSS33 |
MIMi | 613797, gene |
neXtProti | NX_Q8NF86 |
VEuPathDBi | HostDB:ENSG00000103355.12 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 260429 |
OpenTargetsi | ENSG00000103355 |
PharmGKBi | PA134987813 |
Miscellaneous databases
Pharosi | Q8NF86, Tbio |
Genetic variation databases
BioMutai | PRSS33 |
DMDMi | 212286047 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000299316 | 23 – 280 | Serine protease 33Add BLAST | 258 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 62 ↔ 78 | PROSITE-ProRule annotation | ||
Disulfide bondi | 160 ↔ 237 | PROSITE-ProRule annotation | ||
Disulfide bondi | 193 ↔ 216 | PROSITE-ProRule annotation | ||
Disulfide bondi | 227 ↔ 255 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | Q8NF86 |
PaxDbi | Q8NF86 |
PeptideAtlasi | Q8NF86 |
PRIDEi | Q8NF86 |
ProteomicsDBi | 73267 |
PTM databases
iPTMneti | Q8NF86 |
PhosphoSitePlusi | Q8NF86 |
Expressioni
Tissue specificityi
Predominantly expressed in macrophages. Present in the spleen, small and large intestine, lung and brain (at protein level). Highly expressed in peripheral leukocytes, ovary, retina, spleen and stomach. Moderately expressed in thymus, uterus and platelets, as well as some brain tissues, such as thalamus and fetal brain.1 Publication
Inductioni
Up-regulated by phorbol myristate acetate (PMA).1 Publication
Gene expression databases
Bgeei | ENSG00000103355, Expressed in pigmented layer of retina and 65 other tissues |
ExpressionAtlasi | Q8NF86, baseline and differential |
Genevisiblei | Q8NF86, HS |
Organism-specific databases
HPAi | ENSG00000103355, Tissue enriched (blood) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 129278, 8 interactors |
IntActi | Q8NF86, 5 interactors |
STRINGi | 9606.ENSP00000293851 |
Miscellaneous databases
RNActi | Q8NF86, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 37 – 279 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 243 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000162519 |
HOGENOMi | CLU_006842_1_2_1 |
InParanoidi | Q8NF86 |
OMAi | WIQAHVS |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q8NF86 |
TreeFami | TF351676 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8NF86-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRGVSCLQVL LLLVLGAAGT QGRKSAACGQ PRMSSRIVGG RDGRDGEWPW
60 70 80 90 100
QASIQHRGAH VCGGSLIAPQ WVLTAAHCFP RRALPAEYRV RLGALRLGST
110 120 130 140 150
SPRTLSVPVR RVLLPPDYSE DGARGDLALL QLRRPVPLSA RVQPVCLPVP
160 170 180 190 200
GARPPPGTPC RVTGWGSLRP GVPLPEWRPL QGVRVPLLDS RTCDGLYHVG
210 220 230 240 250
ADVPQAERIV LPGSLCAGYP QGHKDACQGD SGGPLTCLQS GSWVLVGVVS
260 270 280
WGKGCALPNR PGVYTSVATY SPWIQARVSF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketI3L1S0 | I3L1S0_HUMAN | Serine protease 33 | PRSS33 | 115 | Annotation score: | ||
I3L3D7 | I3L3D7_HUMAN | Serine protease 33 | PRSS33 | 236 | Annotation score: |
Sequence cautioni
The sequence AAN04055 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 57 | R → P in AAN04055 (PubMed:12795636).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF536382 mRNA Translation: AAN04055.1 Frameshift. AC092117 Genomic DNA No translation available. CH471112 Genomic DNA Translation: EAW85469.1 BC036846 mRNA Translation: AAH36846.2 BC062334 mRNA Translation: AAH62334.1 |
CCDSi | CCDS42110.1 |
RefSeqi | NP_690851.2, NM_152891.2 XP_011520753.1, XM_011522451.2 XP_011520754.1, XM_011522452.2 |
Genome annotation databases
Ensembli | ENST00000293851; ENSP00000293851; ENSG00000103355 ENST00000570702; ENSP00000458369; ENSG00000103355 |
GeneIDi | 260429 |
KEGGi | hsa:260429 |
UCSCi | uc002cro.1, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF536382 mRNA Translation: AAN04055.1 Frameshift. AC092117 Genomic DNA No translation available. CH471112 Genomic DNA Translation: EAW85469.1 BC036846 mRNA Translation: AAH36846.2 BC062334 mRNA Translation: AAH62334.1 |
CCDSi | CCDS42110.1 |
RefSeqi | NP_690851.2, NM_152891.2 XP_011520753.1, XM_011522451.2 XP_011520754.1, XM_011522452.2 |
3D structure databases
SMRi | Q8NF86 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 129278, 8 interactors |
IntActi | Q8NF86, 5 interactors |
STRINGi | 9606.ENSP00000293851 |
Protein family/group databases
MEROPSi | S01.075 |
PTM databases
iPTMneti | Q8NF86 |
PhosphoSitePlusi | Q8NF86 |
Genetic variation databases
BioMutai | PRSS33 |
DMDMi | 212286047 |
Proteomic databases
jPOSTi | Q8NF86 |
PaxDbi | Q8NF86 |
PeptideAtlasi | Q8NF86 |
PRIDEi | Q8NF86 |
ProteomicsDBi | 73267 |
Protocols and materials databases
Antibodypediai | 62205, 80 antibodies |
Genome annotation databases
Ensembli | ENST00000293851; ENSP00000293851; ENSG00000103355 ENST00000570702; ENSP00000458369; ENSG00000103355 |
GeneIDi | 260429 |
KEGGi | hsa:260429 |
UCSCi | uc002cro.1, human |
Organism-specific databases
CTDi | 260429 |
DisGeNETi | 260429 |
GeneCardsi | PRSS33 |
HGNCi | HGNC:30405, PRSS33 |
HPAi | ENSG00000103355, Tissue enriched (blood) |
MIMi | 613797, gene |
neXtProti | NX_Q8NF86 |
OpenTargetsi | ENSG00000103355 |
PharmGKBi | PA134987813 |
VEuPathDBi | HostDB:ENSG00000103355.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000162519 |
HOGENOMi | CLU_006842_1_2_1 |
InParanoidi | Q8NF86 |
OMAi | WIQAHVS |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q8NF86 |
TreeFami | TF351676 |
Enzyme and pathway databases
PathwayCommonsi | Q8NF86 |
Miscellaneous databases
BioGRID-ORCSi | 260429, 10 hits in 871 CRISPR screens |
ChiTaRSi | PRSS33, human |
GenomeRNAii | 260429 |
Pharosi | Q8NF86, Tbio |
PROi | PR:Q8NF86 |
RNActi | Q8NF86, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000103355, Expressed in pigmented layer of retina and 65 other tissues |
ExpressionAtlasi | Q8NF86, baseline and differential |
Genevisiblei | Q8NF86, HS |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRS33_HUMAN | |
Accessioni | Q8NF86Primary (citable) accession number: Q8NF86 Secondary accession number(s): A6NNQ3, Q8N171 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | July 22, 2008 | |
Last modified: | February 10, 2021 | |
This is version 124 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families