UniProtKB - Q8NEM7 (SP20H_HUMAN)
Protein
Transcription factor SPT20 homolog
Gene
SUPT20H
Organism
Homo sapiens (Human)
Status
Functioni
Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by FGF signaling and Snail (By similarity). Required for starvation-induced ATG9A trafficking during autophagy.By similarity1 Publication
GO - Molecular functioni
- transcription coregulator activity Source: InterPro
GO - Biological processi
- autophagy Source: UniProtKB-KW
- gastrulation Source: UniProtKB-KW
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Developmental protein |
Biological process | Autophagy, Gastrulation |
Enzyme and pathway databases
PathwayCommonsi | Q8NEM7 |
Reactomei | R-HSA-3214847, HATs acetylate histones |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor SPT20 homologAlternative name(s): p38-interacting protein Short name: p38IP |
Gene namesi | Name:SUPT20H Synonyms:C13orf19, FAM48A ORF Names:FP757 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000102710.19 |
HGNCi | HGNC:20596, SUPT20H |
MIMi | 613417, gene |
neXtProti | NX_Q8NEM7 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- SAGA complex Source: GO_Central
- SAGA-type complex Source: BHF-UCL
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 55578 |
OpenTargetsi | ENSG00000102710 |
PharmGKBi | PA134985241 |
Miscellaneous databases
Pharosi | Q8NEM7, Tbio |
Polymorphism and mutation databases
BioMutai | SUPT20H |
DMDMi | 334302791 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000187039 | 1 – 779 | Transcription factor SPT20 homologAdd BLAST | 779 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 296 | PhosphoserineBy similarity | 1 | |
Modified residuei | 494 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 519 | PhosphoserineCombined sources | 1 | |
Modified residuei | 524 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8NEM7 |
jPOSTi | Q8NEM7 |
MassIVEi | Q8NEM7 |
MaxQBi | Q8NEM7 |
PaxDbi | Q8NEM7 |
PeptideAtlasi | Q8NEM7 |
PRIDEi | Q8NEM7 |
ProteomicsDBi | 17715 73182 [Q8NEM7-1] 73183 [Q8NEM7-2] |
PTM databases
GlyGeni | Q8NEM7, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q8NEM7 |
PhosphoSitePlusi | Q8NEM7 |
Expressioni
Tissue specificityi
Highly expressed in testis, moderately in brain and pituitary gland. Expressed in several fetal tissues, including lung, brain, thymus and kidney. Expression is down-regulated in malignant prostate tissues.1 Publication
Gene expression databases
Bgeei | ENSG00000102710, Expressed in kidney and 235 other tissues |
ExpressionAtlasi | Q8NEM7, baseline and differential |
Genevisiblei | Q8NEM7, HS |
Organism-specific databases
HPAi | ENSG00000102710, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with MAPK14.
Interacts with ATG9A.
2 PublicationsBinary interactionsi
Hide detailsQ8NEM7
With | #Exp. | IntAct |
---|---|---|
MAPK14 [Q16539] | 5 | EBI-946984,EBI-73946 |
Isoform 2 [Q8NEM7-2]
With | #Exp. | IntAct |
---|---|---|
ATG9A [Q7Z3C6] | 8 | EBI-7568679,EBI-727146 |
Protein-protein interaction databases
BioGRIDi | 120729, 38 interactors |
ComplexPortali | CPX-900, SAGA complex |
CORUMi | Q8NEM7 |
IntActi | Q8NEM7, 23 interactors |
Miscellaneous databases
RNActi | Q8NEM7, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 636 – 754 | Gln-richAdd BLAST | 119 |
Sequence similaritiesi
Belongs to the SPT20 family.Curated
Phylogenomic databases
GeneTreei | ENSGT00390000013549 |
HOGENOMi | CLU_020200_1_0_1 |
InParanoidi | Q8NEM7 |
OMAi | EDYVFEC |
OrthoDBi | 369747at2759 |
TreeFami | TF329155 |
Family and domain databases
InterProi | View protein in InterPro IPR021950, Spt20 |
PANTHERi | PTHR13526, PTHR13526, 2 hits |
Pfami | View protein in Pfam PF12090, Spt20, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NEM7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQQALELALD RAEYVIESAR QRPPKRKYLS SGRKSVFQKL YDLYIEECEK
60 70 80 90 100
EPEVKKLRRN VNLLEKLVMQ ETLSCLVVNL YPGNEGYSLM LRGKNGSDSE
110 120 130 140 150
TIRLPYEEGE LLEYLDAEEL PPILVDLLEK SQVNIFHCGC VIAEIRDYRQ
160 170 180 190 200
SSNMKSPGYQ SRHILLRPTM QTLICDVHSI TSDNHKWTQE DKLLLESQLI
210 220 230 240 250
LATAEPLCLD PSIAVTCTAN RLLYNKQKMN TRPMKRCFKR YSRSSLNRQQ
260 270 280 290 300
DLSHCPPPPQ LRLLDFLQKR KERKAGQHYD LKISKAGNCV DMWKRSPCNL
310 320 330 340 350
AIPSEVDVEK YAKVEKSIKS DDSQPTVWPA HDVKDDYVFE CEAGTQYQKT
360 370 380 390 400
KLTILQSLGD PLYYGKIQPC KADEESDSQM SPSHSSTDDH SNWFIIGSKT
410 420 430 440 450
DAERVVNQYQ ELVQNEAKCP VKMSHSSSGS ASLSQVSPGK ETDQTETVSV
460 470 480 490 500
QSSVLGKGVK HRPPPIKLPS SSGNSSSGNY FTPQQTSSFL KSPTPPPSSK
510 520 530 540 550
PSSIPRKSSV DLNQVSMLSP AALSPASSSQ RTTATQVMAN SAGLNFINVV
560 570 580 590 600
GSVCGAQALM SGSNPMLGCN TGAITPAGIN LSGLLPSGGL LPNALPSAMQ
610 620 630 640 650
AASQAGVPFG LKNTSSLRPL NLLQLPGGSL IFNTLQQQQQ QLSQFTPQQP
660 670 680 690 700
QQPTTCSPQQ PGEQGSEQGS TSQEQALSAQ QAAVINLTGV GSFMQSQAAV
710 720 730 740 750
LSQLGSAENR PEQSLPQQRF QLSSAFQQQQ QQIQQLRFLQ HQMAMAAAAA
760 770
QTAQLHHHRH TGSQSKSKMK RGTPTTPKF
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6S7C4 | F6S7C4_HUMAN | Transcription factor SPT20 homolog | SUPT20H | 736 | Annotation score: | ||
B4E2D5 | B4E2D5_HUMAN | Transcription factor SPT20 homolog | SUPT20H | 498 | Annotation score: | ||
H7C5F9 | H7C5F9_HUMAN | Transcription factor SPT20 homolog | SUPT20H | 378 | Annotation score: | ||
R4GND2 | R4GND2_HUMAN | Transcription factor SPT20 homolog | SUPT20H | 522 | Annotation score: | ||
C9JQS2 | C9JQS2_HUMAN | Transcription factor SPT20 homolog | SUPT20H | 190 | Annotation score: |
Sequence cautioni
The sequence AAQ15220 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 707 | A → V in AAH30686 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055798 | 154 | M → V. Corresponds to variant dbSNP:rs7324275Ensembl. | 1 | |
Natural variantiVAR_055799 | 773 | T → M1 PublicationCorresponds to variant dbSNP:rs9469Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_014877 | 55 | K → KQ in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_055621 | 531 | R → RSGTPKPSTPTPTPSSTPHP PDAQSSTPSTPSATPTPQDS GFTPQPTLLTQFAQQQRSLS QAMPVTTIPLSTMVTSITPG in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_014878 | 700 – 732 | VLSQL…QQQQQ → AVAILAASNGYGSSSSTNSS ATSSSAYRQPVKK in isoform 2 and isoform 3. 3 PublicationsAdd BLAST | 33 | |
Alternative sequenceiVSP_014879 | 733 – 779 | Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST | 47 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ130894 mRNA Translation: CAB62207.1 AF093250 mRNA Translation: AAD40550.1 AK225376 mRNA No translation available. AL138706 Genomic DNA No translation available. AL391383 Genomic DNA No translation available. BC030686 mRNA Translation: AAH30686.1 AF370384 mRNA Translation: AAQ15220.1 Sequence problems. |
CCDSi | CCDS31959.1 [Q8NEM7-1] CCDS61311.1 [Q8NEM7-3] CCDS9362.1 [Q8NEM7-2] |
RefSeqi | NP_001014308.2, NM_001014286.2 [Q8NEM7-1] NP_001265409.1, NM_001278480.1 [Q8NEM7-3] NP_001265410.1, NM_001278481.1 [Q8NEM7-2] NP_001265411.1, NM_001278482.1 [Q8NEM7-2] NP_060039.1, NM_017569.3 [Q8NEM7-2] |
Genome annotation databases
Ensembli | ENST00000350612; ENSP00000218894; ENSG00000102710 [Q8NEM7-1] ENST00000356185; ENSP00000348512; ENSG00000102710 [Q8NEM7-2] ENST00000360252; ENSP00000353388; ENSG00000102710 [Q8NEM7-2] ENST00000464744; ENSP00000419754; ENSG00000102710 [Q8NEM7-2] ENST00000475892; ENSP00000417510; ENSG00000102710 [Q8NEM7-3] |
GeneIDi | 55578 |
KEGGi | hsa:55578 |
UCSCi | uc001uwg.4, human [Q8NEM7-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ130894 mRNA Translation: CAB62207.1 AF093250 mRNA Translation: AAD40550.1 AK225376 mRNA No translation available. AL138706 Genomic DNA No translation available. AL391383 Genomic DNA No translation available. BC030686 mRNA Translation: AAH30686.1 AF370384 mRNA Translation: AAQ15220.1 Sequence problems. |
CCDSi | CCDS31959.1 [Q8NEM7-1] CCDS61311.1 [Q8NEM7-3] CCDS9362.1 [Q8NEM7-2] |
RefSeqi | NP_001014308.2, NM_001014286.2 [Q8NEM7-1] NP_001265409.1, NM_001278480.1 [Q8NEM7-3] NP_001265410.1, NM_001278481.1 [Q8NEM7-2] NP_001265411.1, NM_001278482.1 [Q8NEM7-2] NP_060039.1, NM_017569.3 [Q8NEM7-2] |
3D structure databases
SMRi | Q8NEM7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 120729, 38 interactors |
ComplexPortali | CPX-900, SAGA complex |
CORUMi | Q8NEM7 |
IntActi | Q8NEM7, 23 interactors |
PTM databases
GlyGeni | Q8NEM7, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q8NEM7 |
PhosphoSitePlusi | Q8NEM7 |
Polymorphism and mutation databases
BioMutai | SUPT20H |
DMDMi | 334302791 |
Proteomic databases
EPDi | Q8NEM7 |
jPOSTi | Q8NEM7 |
MassIVEi | Q8NEM7 |
MaxQBi | Q8NEM7 |
PaxDbi | Q8NEM7 |
PeptideAtlasi | Q8NEM7 |
PRIDEi | Q8NEM7 |
ProteomicsDBi | 17715 73182 [Q8NEM7-1] 73183 [Q8NEM7-2] |
Protocols and materials databases
Antibodypediai | 49228, 74 antibodies |
DNASUi | 55578 |
Genome annotation databases
Ensembli | ENST00000350612; ENSP00000218894; ENSG00000102710 [Q8NEM7-1] ENST00000356185; ENSP00000348512; ENSG00000102710 [Q8NEM7-2] ENST00000360252; ENSP00000353388; ENSG00000102710 [Q8NEM7-2] ENST00000464744; ENSP00000419754; ENSG00000102710 [Q8NEM7-2] ENST00000475892; ENSP00000417510; ENSG00000102710 [Q8NEM7-3] |
GeneIDi | 55578 |
KEGGi | hsa:55578 |
UCSCi | uc001uwg.4, human [Q8NEM7-1] |
Organism-specific databases
CTDi | 55578 |
DisGeNETi | 55578 |
EuPathDBi | HostDB:ENSG00000102710.19 |
GeneCardsi | SUPT20H |
HGNCi | HGNC:20596, SUPT20H |
HPAi | ENSG00000102710, Low tissue specificity |
MIMi | 613417, gene |
neXtProti | NX_Q8NEM7 |
OpenTargetsi | ENSG00000102710 |
PharmGKBi | PA134985241 |
GenAtlasi | Search... |
Phylogenomic databases
GeneTreei | ENSGT00390000013549 |
HOGENOMi | CLU_020200_1_0_1 |
InParanoidi | Q8NEM7 |
OMAi | EDYVFEC |
OrthoDBi | 369747at2759 |
TreeFami | TF329155 |
Enzyme and pathway databases
PathwayCommonsi | Q8NEM7 |
Reactomei | R-HSA-3214847, HATs acetylate histones |
Miscellaneous databases
BioGRID-ORCSi | 55578, 103 hits in 857 CRISPR screens |
ChiTaRSi | SUPT20H, human |
GeneWikii | FAM48A |
GenomeRNAii | 55578 |
Pharosi | Q8NEM7, Tbio |
PROi | PR:Q8NEM7 |
RNActi | Q8NEM7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000102710, Expressed in kidney and 235 other tissues |
ExpressionAtlasi | Q8NEM7, baseline and differential |
Genevisiblei | Q8NEM7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR021950, Spt20 |
PANTHERi | PTHR13526, PTHR13526, 2 hits |
Pfami | View protein in Pfam PF12090, Spt20, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SP20H_HUMAN | |
Accessioni | Q8NEM7Primary (citable) accession number: Q8NEM7 Secondary accession number(s): E7ER46, Q71RF3, Q9Y6A6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | May 31, 2011 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 13
Human chromosome 13: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations