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Protein

Dual specificity protein phosphatase 18

Gene

DUSP18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. In vitro, dephosphorylates p-nitrophenyl phosphate (pNPP).2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation2 Publications
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.2 Publications

Activity regulationi

Activated by manganese ions, inhibited by iodoaretic acid.1 Publication

pH dependencei

Optimum pH is 6.0.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 18 (EC:3.1.3.162 Publications, EC:3.1.3.482 Publications)
Alternative name(s):
Low molecular weight dual specificity phosphatase 201 Publication
Short name:
LMW-DSP20
Gene namesi
Name:DUSP18
Synonyms:LMWDSP20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000167065.13
HGNCiHGNC:18484 DUSP18
MIMi611446 gene
neXtProtiNX_Q8NEJ0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73D → A: Abolishes most of in vitro phosphatase activity. 1 Publication1
Mutagenesisi102L → V: No effect on in vitro phosphatase activity. 1 Publication1
Mutagenesisi104C → S: Abolishes most of in vitro phosphatase activity. 1 Publication1
Mutagenesisi110R → K: Abolishes most of in vitro phosphatase activity. 1 Publication1
Mutagenesisi111S → A: Abolishes most of in vitro phosphatase activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000167065
PharmGKBiPA134928498

Polymorphism and mutation databases

BioMutaiDUSP18
DMDMi29840768

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948281 – 188Dual specificity protein phosphatase 18Add BLAST188

Proteomic databases

MaxQBiQ8NEJ0
PaxDbiQ8NEJ0
PeptideAtlasiQ8NEJ0
PRIDEiQ8NEJ0
ProteomicsDBi73168

PTM databases

DEPODiQ8NEJ0
PhosphoSitePlusiQ8NEJ0

Expressioni

Tissue specificityi

Widely expressed with highest levels in liver, brain, ovary and testis.2 Publications

Gene expression databases

BgeeiENSG00000167065 Expressed in 168 organ(s), highest expression level in secondary oocyte
CleanExiHS_DUSP18
ExpressionAtlasiQ8NEJ0 baseline and differential
GenevisibleiQ8NEJ0 HS

Organism-specific databases

HPAiCAB034070
HPA051349

Interactioni

Protein-protein interaction databases

BioGridi127280, 20 interactors
IntActiQ8NEJ0, 1 interactor
STRINGi9606.ENSP00000333917

Structurei

Secondary structure

1188
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8NEJ0
SMRiQ8NEJ0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NEJ0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 149Tyrosine-protein phosphataseAdd BLAST70

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1718 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118853
HOGENOMiHOG000233766
HOVERGENiHBG051422
InParanoidiQ8NEJ0
KOiK14165
OMAiPEYRAME
OrthoDBiEOG091G0249
PhylomeDBiQ8NEJ0
TreeFamiTF316009

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR020417 Atypical_DUSP
IPR020420 Atypical_DUSP_famB
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PRINTSiPR01908 ADSPHPHTASE
PR01910 ADSPHPHTASEB
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q8NEJ0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTAPSCAFPV QFRQPSVSGL SQITKSLYIS NGVAANNKLM LSSNQITMVI
60 70 80 90 100
NVSVEVVNTL YEDIQYMQVP VADSPNSRLC DFFDPIADHI HSVEMKQGRT
110 120 130 140 150
LLHCAAGVSR SAALCLAYLM KYHAMSLLDA HTWTKSCRPI IRPNSGFWEQ
160 170 180
LIHYEFQLFG KNTVHMVSSP VGMIPDIYEK EVRLMIPL
Length:188
Mass (Da):21,066
Last modified:October 1, 2002 - v1
Checksum:iA6EDECC3624F570F
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VX42F8VX42_HUMAN
Dual-specificity protein phosphatas...
DUSP18
163Annotation score:
F2Z2P2F2Z2P2_HUMAN
Dual-specificity protein phosphatas...
DUSP18
117Annotation score:
H7C401H7C401_HUMAN
Dual-specificity protein phosphatas...
DUSP18
23Annotation score:
C9JEE9C9JEE9_HUMAN
Dual-specificity protein phosphatas...
DUSP18
11Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2T → A in AAN77931 (PubMed:12591617).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF533017 mRNA Translation: AAN59787.1
AF461689 mRNA Translation: AAN77931.1
CR456406 mRNA Translation: CAG30292.1
AK056074 mRNA Translation: BAG51616.1
CH471095 Genomic DNA Translation: EAW59913.1
BC030987 mRNA Translation: AAH30987.1
CCDSiCCDS13883.1
RefSeqiNP_001291723.1, NM_001304794.1
NP_001291724.1, NM_001304795.1
NP_689724.3, NM_152511.4
XP_005261425.1, XM_005261368.4
XP_006724211.1, XM_006724148.3
XP_011528222.1, XM_011529920.2
XP_011528223.1, XM_011529921.2
XP_016884116.1, XM_017028627.1
XP_016884117.1, XM_017028628.1
UniGeneiHs.517544

Genome annotation databases

EnsembliENST00000334679; ENSP00000333917; ENSG00000167065
ENST00000377087; ENSP00000366291; ENSG00000167065
ENST00000404885; ENSP00000385463; ENSG00000167065
ENST00000407308; ENSP00000386063; ENSG00000167065
GeneIDi150290
KEGGihsa:150290
UCSCiuc003aiu.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF533017 mRNA Translation: AAN59787.1
AF461689 mRNA Translation: AAN77931.1
CR456406 mRNA Translation: CAG30292.1
AK056074 mRNA Translation: BAG51616.1
CH471095 Genomic DNA Translation: EAW59913.1
BC030987 mRNA Translation: AAH30987.1
CCDSiCCDS13883.1
RefSeqiNP_001291723.1, NM_001304794.1
NP_001291724.1, NM_001304795.1
NP_689724.3, NM_152511.4
XP_005261425.1, XM_005261368.4
XP_006724211.1, XM_006724148.3
XP_011528222.1, XM_011529920.2
XP_011528223.1, XM_011529921.2
XP_016884116.1, XM_017028627.1
XP_016884117.1, XM_017028628.1
UniGeneiHs.517544

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ESBX-ray2.00A1-188[»]
ProteinModelPortaliQ8NEJ0
SMRiQ8NEJ0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127280, 20 interactors
IntActiQ8NEJ0, 1 interactor
STRINGi9606.ENSP00000333917

PTM databases

DEPODiQ8NEJ0
PhosphoSitePlusiQ8NEJ0

Polymorphism and mutation databases

BioMutaiDUSP18
DMDMi29840768

Proteomic databases

MaxQBiQ8NEJ0
PaxDbiQ8NEJ0
PeptideAtlasiQ8NEJ0
PRIDEiQ8NEJ0
ProteomicsDBi73168

Protocols and materials databases

DNASUi150290
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334679; ENSP00000333917; ENSG00000167065
ENST00000377087; ENSP00000366291; ENSG00000167065
ENST00000404885; ENSP00000385463; ENSG00000167065
ENST00000407308; ENSP00000386063; ENSG00000167065
GeneIDi150290
KEGGihsa:150290
UCSCiuc003aiu.4 human

Organism-specific databases

CTDi150290
EuPathDBiHostDB:ENSG00000167065.13
GeneCardsiDUSP18
HGNCiHGNC:18484 DUSP18
HPAiCAB034070
HPA051349
MIMi611446 gene
neXtProtiNX_Q8NEJ0
OpenTargetsiENSG00000167065
PharmGKBiPA134928498
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1718 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118853
HOGENOMiHOG000233766
HOVERGENiHBG051422
InParanoidiQ8NEJ0
KOiK14165
OMAiPEYRAME
OrthoDBiEOG091G0249
PhylomeDBiQ8NEJ0
TreeFamiTF316009

Miscellaneous databases

EvolutionaryTraceiQ8NEJ0
GeneWikiiDUSP18
GenomeRNAii150290
PROiPR:Q8NEJ0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167065 Expressed in 168 organ(s), highest expression level in secondary oocyte
CleanExiHS_DUSP18
ExpressionAtlasiQ8NEJ0 baseline and differential
GenevisibleiQ8NEJ0 HS

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR020417 Atypical_DUSP
IPR020420 Atypical_DUSP_famB
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PRINTSiPR01908 ADSPHPHTASE
PR01910 ADSPHPHTASEB
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDUS18_HUMAN
AccessioniPrimary (citable) accession number: Q8NEJ0
Secondary accession number(s): B3KPA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 1, 2002
Last modified: November 7, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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