UniProtKB - Q8NEB5 (PLPP5_HUMAN)
Protein
Phospholipid phosphatase 5
Gene
PLPP5
Organism
Homo sapiens (Human)
Status
Functioni
Magnesium-independent phospholipid phosphatase with broad substrate specificity (PubMed:17590538). Preferentially catalyzes the conversion of diacylglycerol pyrophosphate into phosphatidate but can also act on phosphatidate and lysophosphatidate (PubMed:17590538). Phospholipid phosphatases are involved in both the synthesis of lipids and the generation or degradation of lipid-signaling molecules (PubMed:17590538).1 Publication1 Publication
Miscellaneous
May be a metastatic suppressor for hepatocellular carcinoma.1 Publication
Catalytic activityi
- a 1,2-diacyl-sn-glycerol 3-diphosphate + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + H+ + phosphate1 PublicationEC:3.1.3.811 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:3.1.3.41 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1,2-dioctanoyl-sn-glycero-3-diphosphate + H2O = 1,2-dioctanoyl-sn-glycero-3-phosphate + H+ + phosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O = 1-(9Z-octadecenoyl)-sn-glycerol + phosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Magnesium-independent phospholipid phosphatase (PubMed:17590538). Inhibited by N-ethylmaleimide (PubMed:17590538).1 Publication
Kineticsi
- KM=41 µM for 1,2-dioctanoyl-sn-glycero-3-diphosphate/diacylglycerol pyrophosphate1 Publication
- KM=277 µM for 1,2-dioctanoyl-sn-glycero-3-phosphate/phosphatidate1 Publication
- KM=295 µM for 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate/lysophosphatidate1 Publication
: phospholipid metabolism Pathwayi
This protein is involved in the pathway phospholipid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway phospholipid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 152 | Proton donorsBy similarity | 1 | |
Active sitei | 208 | NucleophileBy similarity | 1 | |
Sitei | 212 | Stabilizes the active site histidine for nucleophilic attackBy similarity | 1 |
GO - Molecular functioni
- diacylglycerol diphosphate phosphatase activity Source: UniProtKB
- phosphatase activity Source: GO_Central
- phosphatidate phosphatase activity Source: UniProtKB
GO - Biological processi
- phospholipid dephosphorylation Source: UniProtKB
- phospholipid metabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 3.1.3.4, 2681 3.1.3.81, 2681 |
PathwayCommonsi | Q8NEB5 |
Reactomei | R-HSA-2029485, Role of phospholipids in phagocytosis |
UniPathwayi | UPA00085 |
Chemistry databases
SwissLipidsi | SLP:000000604 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25026, PLPP5 |
MIMi | 610626, gene |
neXtProti | NX_Q8NEB5 |
VEuPathDBi | HostDB:ENSG00000147535.16 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 5 | CytoplasmicCurated | 5 | |
Transmembranei | 6 – 26 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 27 – 54 | ExtracellularBy similarityAdd BLAST | 28 | |
Transmembranei | 55 – 75 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 76 – 90 | CytoplasmicBy similarityAdd BLAST | 15 | |
Transmembranei | 91 – 111 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 112 – 154 | ExtracellularBy similarityAdd BLAST | 43 | |
Transmembranei | 155 – 175 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 176 – 179 | CytoplasmicBy similarity | 4 | |
Transmembranei | 180 – 200 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 201 – 208 | ExtracellularBy similarity | 8 | |
Transmembranei | 209 – 228 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 229 – 264 | CytoplasmicBy similarityAdd BLAST | 36 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 115 | R → A: Loss of phosphatase activity; when associated with A-152 and with A-208. 1 Publication | 1 | |
Mutagenesisi | 152 | H → A: Loss of phosphatase activity; when associated with A-115 and with A-208. 1 Publication | 1 | |
Mutagenesisi | 208 | H → A: Loss of phosphatase activity; when associated with A-115 and with A-152. 1 Publication | 1 |
Keywords - Diseasei
Tumor suppressorOrganism-specific databases
DisGeNETi | 84513 |
OpenTargetsi | ENSG00000147535 |
PharmGKBi | PA142671154 |
Miscellaneous databases
Pharosi | Q8NEB5, Tbio |
Genetic variation databases
BioMutai | PLPP5 |
DMDMi | 313104170 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000286945 | 1 – 264 | Phospholipid phosphatase 5Add BLAST | 264 |
Proteomic databases
EPDi | Q8NEB5 |
jPOSTi | Q8NEB5 |
MassIVEi | Q8NEB5 |
MaxQBi | Q8NEB5 |
PaxDbi | Q8NEB5 |
PeptideAtlasi | Q8NEB5 |
PRIDEi | Q8NEB5 |
ProteomicsDBi | 10577 73143 [Q8NEB5-1] 73144 [Q8NEB5-2] 73145 [Q8NEB5-3] |
PTM databases
DEPODi | PLPP5 |
iPTMneti | Q8NEB5 |
PhosphoSitePlusi | Q8NEB5 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSG00000147535, Expressed in body of pancreas and 194 other tissues |
ExpressionAtlasi | Q8NEB5, baseline and differential |
Genevisiblei | Q8NEB5, HS |
Organism-specific databases
HPAi | ENSG00000147535, Tissue enhanced (pancreas) |
Interactioni
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 124107, 3 interactors |
IntActi | Q8NEB5, 3 interactors |
STRINGi | 9606.ENSP00000392553 |
Miscellaneous databases
RNActi | Q8NEB5, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 108 – 116 | Phosphatase sequence motif I1 Publication | 9 | |
Regioni | 149 – 152 | Phosphatase sequence motif II1 Publication | 4 | |
Regioni | 201 – 211 | Phosphatase sequence motif III1 PublicationAdd BLAST | 11 |
Sequence similaritiesi
Belongs to the PA-phosphatase related phosphoesterase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3030, Eukaryota |
GeneTreei | ENSGT00940000159772 |
InParanoidi | Q8NEB5 |
OMAi | SWAWTLA |
OrthoDBi | 1621899at2759 |
PhylomeDBi | Q8NEB5 |
TreeFami | TF323722 |
Family and domain databases
InterProi | View protein in InterPro IPR036938, P_Acid_Pase_2/haloperoxi_sf IPR000326, P_Acid_Pase_2/haloperoxidase IPR043216, PLPP IPR028668, PLPP4/5 |
PANTHERi | PTHR10165, PTHR10165, 1 hit PTHR10165:SF87, PTHR10165:SF87, 1 hit |
Pfami | View protein in Pfam PF01569, PAP2, 1 hit |
SMARTi | View protein in SMART SM00014, acidPPc, 1 hit |
SUPFAMi | SSF48317, SSF48317, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NEB5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGKAAAAVAF GAEVGVRLAL FAAFLVTELL PPFQRLIQPE EMWLYRNPYV
60 70 80 90 100
EAEYFPTKPM FVIAFLSPLS LIFLAKFLKK ADTRDSRQAC LAASLALALN
110 120 130 140 150
GVFTNTIKLI VGRPRPDFFY RCFPDGLAHS DLMCTGDKDV VNEGRKSFPS
160 170 180 190 200
GHSSFAFAGL AFASFYLAGK LHCFTPQGRG KSWRFCAFLS PLLFAAVIAL
210 220 230 240 250
SRTCDYKHHW QDVLVGSMIG MTFAYVCYRQ YYPPLTDAEC HKPFQDKLVL
260
STAQKPGDSY CFDI
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PNB1 | E9PNB1_HUMAN | Phospholipid phosphatase 5 | PLPP5 | 132 | Annotation score: | ||
H0YCQ3 | H0YCQ3_HUMAN | Phospholipid phosphatase 5 | PLPP5 | 245 | Annotation score: | ||
H0YCK0 | H0YCK0_HUMAN | Phospholipid phosphatase 5 | PLPP5 | 244 | Annotation score: | ||
H0YDY2 | H0YDY2_HUMAN | Phospholipid phosphatase 5 | PLPP5 | 60 | Annotation score: |
Sequence cautioni
The sequence AAI06015 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAK14924 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040055 | 1 – 41 | Missing in isoform 3. 1 PublicationAdd BLAST | 41 | |
Alternative sequenceiVSP_054076 | 155 – 176 | FAFAG…HCFTP → CMNIIWTLPYACFHIIEFNL ES in isoform 4. CuratedAdd BLAST | 22 | |
Alternative sequenceiVSP_054077 | 177 – 264 | Missing in isoform 4. CuratedAdd BLAST | 88 | |
Alternative sequenceiVSP_025241 | 212 – 216 | DVLVG → GPFKW in isoform 2. 2 Publications | 5 | |
Alternative sequenceiVSP_025242 | 217 – 264 | Missing in isoform 2. 2 PublicationsAdd BLAST | 48 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BD418664 mRNA No translation available. AC087362 Genomic DNA No translation available. BC033025 mRNA Translation: AAH33025.1 BC106014 mRNA Translation: AAI06015.2 Different initiation. AF212238 mRNA Translation: AAK14924.1 Different initiation. |
CCDSi | CCDS47841.1 [Q8NEB5-1] CCDS47842.1 [Q8NEB5-2] CCDS47843.1 [Q8NEB5-4] |
RefSeqi | NP_001096029.1, NM_001102559.1 [Q8NEB5-1] NP_001096030.1, NM_001102560.1 [Q8NEB5-4] NP_115872.2, NM_032483.3 [Q8NEB5-2] XP_011542973.1, XM_011544671.1 [Q8NEB5-3] |
Genome annotation databases
Ensembli | ENST00000419686; ENSP00000414522; ENSG00000147535 [Q8NEB5-4] ENST00000422581; ENSP00000390622; ENSG00000147535 [Q8NEB5-2] ENST00000424479; ENSP00000392553; ENSG00000147535 [Q8NEB5-1] ENST00000529359; ENSP00000434916; ENSG00000147535 [Q8NEB5-3] |
GeneIDi | 84513 |
KEGGi | hsa:84513 |
UCSCi | uc003xle.5, human [Q8NEB5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BD418664 mRNA No translation available. AC087362 Genomic DNA No translation available. BC033025 mRNA Translation: AAH33025.1 BC106014 mRNA Translation: AAI06015.2 Different initiation. AF212238 mRNA Translation: AAK14924.1 Different initiation. |
CCDSi | CCDS47841.1 [Q8NEB5-1] CCDS47842.1 [Q8NEB5-2] CCDS47843.1 [Q8NEB5-4] |
RefSeqi | NP_001096029.1, NM_001102559.1 [Q8NEB5-1] NP_001096030.1, NM_001102560.1 [Q8NEB5-4] NP_115872.2, NM_032483.3 [Q8NEB5-2] XP_011542973.1, XM_011544671.1 [Q8NEB5-3] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 124107, 3 interactors |
IntActi | Q8NEB5, 3 interactors |
STRINGi | 9606.ENSP00000392553 |
Chemistry databases
SwissLipidsi | SLP:000000604 |
PTM databases
DEPODi | PLPP5 |
iPTMneti | Q8NEB5 |
PhosphoSitePlusi | Q8NEB5 |
Genetic variation databases
BioMutai | PLPP5 |
DMDMi | 313104170 |
Proteomic databases
EPDi | Q8NEB5 |
jPOSTi | Q8NEB5 |
MassIVEi | Q8NEB5 |
MaxQBi | Q8NEB5 |
PaxDbi | Q8NEB5 |
PeptideAtlasi | Q8NEB5 |
PRIDEi | Q8NEB5 |
ProteomicsDBi | 10577 73143 [Q8NEB5-1] 73144 [Q8NEB5-2] 73145 [Q8NEB5-3] |
Protocols and materials databases
Antibodypediai | 23567, 98 antibodies |
DNASUi | 84513 |
Genome annotation databases
Ensembli | ENST00000419686; ENSP00000414522; ENSG00000147535 [Q8NEB5-4] ENST00000422581; ENSP00000390622; ENSG00000147535 [Q8NEB5-2] ENST00000424479; ENSP00000392553; ENSG00000147535 [Q8NEB5-1] ENST00000529359; ENSP00000434916; ENSG00000147535 [Q8NEB5-3] |
GeneIDi | 84513 |
KEGGi | hsa:84513 |
UCSCi | uc003xle.5, human [Q8NEB5-1] |
Organism-specific databases
CTDi | 84513 |
DisGeNETi | 84513 |
GeneCardsi | PLPP5 |
HGNCi | HGNC:25026, PLPP5 |
HPAi | ENSG00000147535, Tissue enhanced (pancreas) |
MIMi | 610626, gene |
neXtProti | NX_Q8NEB5 |
OpenTargetsi | ENSG00000147535 |
PharmGKBi | PA142671154 |
VEuPathDBi | HostDB:ENSG00000147535.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3030, Eukaryota |
GeneTreei | ENSGT00940000159772 |
InParanoidi | Q8NEB5 |
OMAi | SWAWTLA |
OrthoDBi | 1621899at2759 |
PhylomeDBi | Q8NEB5 |
TreeFami | TF323722 |
Enzyme and pathway databases
UniPathwayi | UPA00085 |
BRENDAi | 3.1.3.4, 2681 3.1.3.81, 2681 |
PathwayCommonsi | Q8NEB5 |
Reactomei | R-HSA-2029485, Role of phospholipids in phagocytosis |
Miscellaneous databases
BioGRID-ORCSi | 84513, 6 hits in 870 CRISPR screens |
ChiTaRSi | PLPP5, human |
GenomeRNAii | 84513 |
Pharosi | Q8NEB5, Tbio |
PROi | PR:Q8NEB5 |
RNActi | Q8NEB5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000147535, Expressed in body of pancreas and 194 other tissues |
ExpressionAtlasi | Q8NEB5, baseline and differential |
Genevisiblei | Q8NEB5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR036938, P_Acid_Pase_2/haloperoxi_sf IPR000326, P_Acid_Pase_2/haloperoxidase IPR043216, PLPP IPR028668, PLPP4/5 |
PANTHERi | PTHR10165, PTHR10165, 1 hit PTHR10165:SF87, PTHR10165:SF87, 1 hit |
Pfami | View protein in Pfam PF01569, PAP2, 1 hit |
SMARTi | View protein in SMART SM00014, acidPPc, 1 hit |
SUPFAMi | SSF48317, SSF48317, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLPP5_HUMAN | |
Accessioni | Q8NEB5Primary (citable) accession number: Q8NEB5 Secondary accession number(s): C9JKF5, Q3KQX6, Q9BY45 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | November 30, 2010 | |
Last modified: | February 10, 2021 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families