UniProtKB - Q8NE71 (ABCF1_HUMAN)
Protein
ATP-binding cassette sub-family F member 1
Gene
ABCF1
Organism
Homo sapiens (Human)
Status
Functioni
Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 336 – 343 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 658 – 665 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATP binding Source: UniProtKB
- ribosome binding Source: UniProtKB
- RNA binding Source: UniProtKB
- translation activator activity Source: UniProtKB
- translation factor activity, RNA binding Source: ProtInc
GO - Biological processi
- inflammatory response Source: ProtInc
- positive regulation of translation Source: UniProtKB
- translation Source: ProtInc
- translational initiation Source: UniProtKB
- transmembrane transport Source: Reactome
Keywordsi
Molecular function | Activator |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q8NE71 |
Reactomei | R-HSA-382556, ABC-family proteins mediated transport |
SIGNORi | Q8NE71 |
Protein family/group databases
TCDBi | 3.A.1.121.8, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-binding cassette sub-family F member 1Alternative name(s): ATP-binding cassette 50 TNF-alpha-stimulated ABC protein |
Gene namesi | Name:ABCF1 Synonyms:ABC50 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:70, ABCF1 |
MIMi | 603429, gene |
neXtProti | NX_Q8NE71 |
VEuPathDBi | HostDB:ENSG00000204574.12 |
Subcellular locationi
Nucleus
- nucleoplasm 1 Publication
- Nucleus envelope 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear envelope Source: UniProtKB
- nucleoplasm Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- membrane Source: UniProtKB
- polysomal ribosome Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 109 | S → A: Reduces phosphorylation. Inhibits strongly phosphorylation by CK2; when associated with S-140. Does not inhibit interaction with EIF2; when associated with S-140. Does not inhibit association with ribosomes; when associated with S-140. Reduces EIF2 interaction with ribosomes; when associated with S-140. Does not inhibit protein synthesis; when associated with A-140. 1 Publication | 1 | |
Mutagenesisi | 140 | S → A: Reduces phosphorylation. Inhibits strongly phosphorylation by CK2; when associated with S-109. Does not inhibits interaction with EIF2; when associated with S-109. Does not inhibit association with ribosomes; when associated with S-109. Reduces EIF2 interaction with ribosomes; when associated with S-109. Does not inhibit protein synthesis; when associated with A-109. 1 Publication | 1 | |
Mutagenesisi | 342 | K → M: Does not inhibit ribosome binding. Reduces ATP-binding. Inhibits ATP-binding and reduces protein synthesis; when associated with M-664. Shows an enhanced association with polyribosomes; when associated with M-664. Does not inhibit IRES-mediated protein synthesis; when associated with M-664. 1 Publication | 1 | |
Mutagenesisi | 367 | Q → E: Does not inhibit ribosome binding. 1 Publication | 1 | |
Mutagenesisi | 454 | G → D: Does not inhibit ribosome binding. 1 Publication | 1 | |
Mutagenesisi | 477 | E → Q: Does not inhibit ribosome binding. Reduces protein synthesis; when associated with Q-768. 1 Publication | 1 | |
Mutagenesisi | 506 | H → L: Does not inhibit ribosome binding. 1 Publication | 1 | |
Mutagenesisi | 664 | K → M: Does not inhibit ribosome binding. Reduces ATP-binding. Inhibits ATP-binding and reduces protein synthesis; when associated with M-342. Shows a reduced association with polyribosomes; when associated with M-664. Does not inhibit IRES-mediated protein synthesis; when associated with M-664. 1 Publication | 1 | |
Mutagenesisi | 695 | Q → E: Does not inhibit ribosome binding. 1 Publication | 1 | |
Mutagenesisi | 745 | G → D: Does not inhibit ribosome binding. 1 Publication | 1 | |
Mutagenesisi | 768 | E → Q: Does not inhibit ribosome binding. Reduces protein synthesis; when associated with Q-477. 1 Publication | 1 | |
Mutagenesisi | 797 | H → L: Does not inhibit ribosome binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 23 |
OpenTargetsi | ENSG00000204574 |
PharmGKBi | PA24405 |
Miscellaneous databases
Pharosi | Q8NE71, Tbio |
Genetic variation databases
BioMutai | ABCF1 |
DMDMi | 56417894 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093318 | 1 – 845 | ATP-binding cassette sub-family F member 1Add BLAST | 845 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 22 | PhosphoserineCombined sources | 1 | |
Modified residuei | 24 | PhosphoserineCombined sources | 1 | |
Modified residuei | 105 | PhosphoserineCombined sources | 1 | |
Modified residuei | 108 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 109 | Phosphoserine; by CK2Combined sources1 Publication | 1 | |
Modified residuei | 140 | Phosphoserine; by CK2Combined sources1 Publication | 1 | |
Modified residuei | 166 | PhosphoserineCombined sources | 1 | |
Modified residuei | 228 | PhosphoserineCombined sources | 1 | |
Modified residuei | 595 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
CPTACi | CPTAC-301 CPTAC-302 |
EPDi | Q8NE71 |
jPOSTi | Q8NE71 |
MassIVEi | Q8NE71 |
MaxQBi | Q8NE71 |
PaxDbi | Q8NE71 |
PeptideAtlasi | Q8NE71 |
PRIDEi | Q8NE71 |
ProteomicsDBi | 73130 [Q8NE71-1] 73131 [Q8NE71-2] |
PTM databases
iPTMneti | Q8NE71 |
MetOSitei | Q8NE71 |
PhosphoSitePlusi | Q8NE71 |
SwissPalmi | Q8NE71 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Inductioni
By TNF in cultured synoviocytes.
Gene expression databases
Bgeei | ENSG00000204574, Expressed in sural nerve and 233 other tissues |
ExpressionAtlasi | Q8NE71, baseline and differential |
Genevisiblei | Q8NE71, HS |
Organism-specific databases
HPAi | ENSG00000204574, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Isoform 2 interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Isoform 2 associates (via both ABC transporter domains) with the ribosomes.
1 PublicationProtein-protein interaction databases
BioGRIDi | 106541, 120 interactors |
DIPi | DIP-50666N |
IntActi | Q8NE71, 38 interactors |
MINTi | Q8NE71 |
STRINGi | 9606.ENSP00000313603 |
Miscellaneous databases
RNActi | Q8NE71, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8NE71 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 304 – 548 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 245 | |
Domaini | 625 – 840 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 216 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 141 – 243 | Glu-richAdd BLAST | 103 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0066, Eukaryota |
GeneTreei | ENSGT00940000158329 |
HOGENOMi | CLU_000604_36_5_1 |
InParanoidi | Q8NE71 |
OMAi | FMVTHDR |
PhylomeDBi | Q8NE71 |
TreeFami | TF105207 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NE71-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPKAPKQQPP EPEWIGDGES TSPSDKVVKK GKKDKKIKKT FFEELAVEDK
60 70 80 90 100
QAGEEEKVLK EKEQQQQQQQ QQQKKKRDTR KGRRKKDVDD DGEEKELMER
110 120 130 140 150
LKKLSVPTSD EEDEVPAPKP RGGKKTKGGN VFAALIQDQS EEEEEEEKHP
160 170 180 190 200
PKPAKPEKNR INKAVSEEQQ PALKGKKGKE EKSKGKAKPQ NKFAALDNEE
210 220 230 240 250
EDKEEEIIKE KEPPKQGKEK AKKAEQGSEE EGEGEEEEEE GGESKADDPY
260 270 280 290 300
AHLSKKEKKK LKKQMEYERQ VASLKAANAA ENDFSVSQAE MSSRQAMLEN
310 320 330 340 350
ASDIKLEKFS ISAHGKELFV NADLYIVAGR RYGLVGPNGK GKTTLLKHIA
360 370 380 390 400
NRALSIPPNI DVLLCEQEVV ADETPAVQAV LRADTKRLKL LEEERRLQGQ
410 420 430 440 450
LEQGDDTAAE RLEKVYEELR ATGAAAAEAK ARRILAGLGF DPEMQNRPTQ
460 470 480 490 500
KFSGGWRMRV SLARALFMEP TLLMLDEPTN HLDLNAVIWL NNYLQGWRKT
510 520 530 540 550
LLIVSHDQGF LDDVCTDIIH LDAQRLHYYR GNYMTFKKMY QQKQKELLKQ
560 570 580 590 600
YEKQEKKLKE LKAGGKSTKQ AEKQTKEALT RKQQKCRRKN QDEESQEAPE
610 620 630 640 650
LLKRPKEYTV RFTFPDPPPL SPPVLGLHGV TFGYQGQKPL FKNLDFGIDM
660 670 680 690 700
DSRICIVGPN GVGKSTLLLL LTGKLTPTHG EMRKNHRLKI GFFNQQYAEQ
710 720 730 740 750
LRMEETPTEY LQRGFNLPYQ DARKCLGRFG LESHAHTIQI CKLSGGQKAR
760 770 780 790 800
VVFAELACRE PDVLILDEPT NNLDIESIDA LGEAINEYKG AVIVVSHDAR
810 820 830 840
LITETNCQLW VVEEQSVSQI DGDFEDYKRE VLEALGEVMV SRPRE
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5GYK6 | F5GYK6_HUMAN | ATP-binding cassette sub-family F m... | ABCF1 | 105 | Annotation score: | ||
H0YGW7 | H0YGW7_HUMAN | ATP-binding cassette sub-family F m... | ABCF1 | 596 | Annotation score: | ||
Q5STZ8 | Q5STZ8_HUMAN | ATP-binding cassette sub-family F m... | ABCF1 | 846 | Annotation score: | ||
A0A0G2JIC2 | A0A0G2JIC2_HUMAN | ATP-binding cassette sub-family F m... | ABCF1 | 238 | Annotation score: | ||
A0A0G2JJB9 | A0A0G2JJB9_HUMAN | ATP-binding cassette sub-family F m... | ABCF1 | 339 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 166 | S → P in AAH34488 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048136 | 198 | N → D. Corresponds to variant dbSNP:rs6902544Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012078 | 226 – 263 | Missing in isoform 2. 1 PublicationAdd BLAST | 38 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF027302 mRNA Translation: AAC70891.1 BA000025 Genomic DNA Translation: BAB63325.1 AB088096 Genomic DNA Translation: BAC54928.1 AL662800 Genomic DNA No translation available. AL662825 Genomic DNA No translation available. BX000357 Genomic DNA No translation available. BX119957 Genomic DNA No translation available. BX248518 Genomic DNA No translation available. CR753328 Genomic DNA No translation available. CR388372 Genomic DNA No translation available. BX927220 Genomic DNA No translation available. CR759778 Genomic DNA No translation available. CR847863 Genomic DNA No translation available. BC034488 mRNA Translation: AAH34488.1 BC112923 mRNA Translation: AAI12924.1 AL832430 mRNA Translation: CAH10648.1 |
CCDSi | CCDS34380.1 [Q8NE71-1] CCDS34381.1 [Q8NE71-2] |
RefSeqi | NP_001020262.1, NM_001025091.1 [Q8NE71-1] NP_001081.1, NM_001090.2 [Q8NE71-2] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
ABCMdb Database for mutations in ABC proteins |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF027302 mRNA Translation: AAC70891.1 BA000025 Genomic DNA Translation: BAB63325.1 AB088096 Genomic DNA Translation: BAC54928.1 AL662800 Genomic DNA No translation available. AL662825 Genomic DNA No translation available. BX000357 Genomic DNA No translation available. BX119957 Genomic DNA No translation available. BX248518 Genomic DNA No translation available. CR753328 Genomic DNA No translation available. CR388372 Genomic DNA No translation available. BX927220 Genomic DNA No translation available. CR759778 Genomic DNA No translation available. CR847863 Genomic DNA No translation available. BC034488 mRNA Translation: AAH34488.1 BC112923 mRNA Translation: AAI12924.1 AL832430 mRNA Translation: CAH10648.1 |
CCDSi | CCDS34380.1 [Q8NE71-1] CCDS34381.1 [Q8NE71-2] |
RefSeqi | NP_001020262.1, NM_001025091.1 [Q8NE71-1] NP_001081.1, NM_001090.2 [Q8NE71-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5ZXD | X-ray | 2.29 | A/B | 300-841 | [»] | |
SMRi | Q8NE71 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106541, 120 interactors |
DIPi | DIP-50666N |
IntActi | Q8NE71, 38 interactors |
MINTi | Q8NE71 |
STRINGi | 9606.ENSP00000313603 |
Protein family/group databases
TCDBi | 3.A.1.121.8, the atp-binding cassette (abc) superfamily |
PTM databases
iPTMneti | Q8NE71 |
MetOSitei | Q8NE71 |
PhosphoSitePlusi | Q8NE71 |
SwissPalmi | Q8NE71 |
Genetic variation databases
BioMutai | ABCF1 |
DMDMi | 56417894 |
Proteomic databases
CPTACi | CPTAC-301 CPTAC-302 |
EPDi | Q8NE71 |
jPOSTi | Q8NE71 |
MassIVEi | Q8NE71 |
MaxQBi | Q8NE71 |
PaxDbi | Q8NE71 |
PeptideAtlasi | Q8NE71 |
PRIDEi | Q8NE71 |
ProteomicsDBi | 73130 [Q8NE71-1] 73131 [Q8NE71-2] |
Protocols and materials databases
Antibodypediai | 2796, 291 antibodies |
DNASUi | 23 |
Genome annotation databases
Organism-specific databases
CTDi | 23 |
DisGeNETi | 23 |
GeneCardsi | ABCF1 |
HGNCi | HGNC:70, ABCF1 |
HPAi | ENSG00000204574, Tissue enhanced (skeletal) |
MIMi | 603429, gene |
neXtProti | NX_Q8NE71 |
OpenTargetsi | ENSG00000204574 |
PharmGKBi | PA24405 |
VEuPathDBi | HostDB:ENSG00000204574.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0066, Eukaryota |
GeneTreei | ENSGT00940000158329 |
HOGENOMi | CLU_000604_36_5_1 |
InParanoidi | Q8NE71 |
OMAi | FMVTHDR |
PhylomeDBi | Q8NE71 |
TreeFami | TF105207 |
Enzyme and pathway databases
PathwayCommonsi | Q8NE71 |
Reactomei | R-HSA-382556, ABC-family proteins mediated transport |
SIGNORi | Q8NE71 |
Miscellaneous databases
BioGRID-ORCSi | 23, 674 hits in 1003 CRISPR screens |
ChiTaRSi | ABCF1, human |
GeneWikii | ABCF1 |
GenomeRNAii | 23 |
Pharosi | Q8NE71, Tbio |
PROi | PR:Q8NE71 |
RNActi | Q8NE71, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204574, Expressed in sural nerve and 233 other tissues |
ExpressionAtlasi | Q8NE71, baseline and differential |
Genevisiblei | Q8NE71, HS |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ABCF1_HUMAN | |
Accessioni | Q8NE71Primary (citable) accession number: Q8NE71 Secondary accession number(s): A2BF75, O14897, Q69YP6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 7, 2004 |
Last sequence update: | December 7, 2004 | |
Last modified: | April 7, 2021 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families