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Protein

Putative RNA-binding protein 15B

Gene

RBM15B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA (PubMed:16129689, PubMed:27602518). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:27602518). Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex (PubMed:27602518). Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist (PubMed:27602518). Functions in the regulation of alternative or illicit splicing, possibly by regulating m6A methylation (PubMed:16129689). Inhibits pre-mRNA splicing (PubMed:21044963). Also functions as a mRNA export factor by acting as a cofactor for the nuclear export receptor NXF1 (PubMed:19586903).1 Publication3 Publications

GO - Molecular functioni

  • nucleic acid binding Source: GO_Central
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, mRNA processing, mRNA splicing, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Putative RNA-binding protein 15BCurated
Alternative name(s):
One-twenty two protein 32 Publications
Short name:
HsOTT31 Publication
Short name:
HuOTT31 Publication
RNA-binding motif protein 15B1 Publication
Gene namesi
Name:RBM15B2 PublicationsImported
Synonyms:OTT32 Publications
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000259956.1
HGNCiHGNC:24303 RBM15B
MIMi612602 gene
neXtProtiNX_Q8NDT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000259956
PharmGKBiPA134870079

Polymorphism and mutation databases

BioMutaiRBM15B
DMDMi229463030

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817781 – 890Putative RNA-binding protein 15BAdd BLAST890

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei109PhosphoserineCombined sources1
Modified residuei113PhosphoserineBy similarity1
Cross-linki213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei265PhosphoserineBy similarity1
Modified residuei267PhosphoserineCombined sources1
Modified residuei532PhosphothreonineCombined sources1
Modified residuei552PhosphoserineCombined sources1
Modified residuei556PhosphoserineCombined sources1
Modified residuei562PhosphoserineCombined sources1
Cross-linki702Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8NDT2
MaxQBiQ8NDT2
PaxDbiQ8NDT2
PeptideAtlasiQ8NDT2
PRIDEiQ8NDT2
ProteomicsDBi73053

PTM databases

iPTMnetiQ8NDT2
PhosphoSitePlusiQ8NDT2

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000259956
CleanExiHS_RBM15B
GenevisibleiQ8NDT2 HS

Organism-specific databases

HPAiHPA036645
HPA058136

Interactioni

Subunit structurei

Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM (PubMed:27602518). The MAC subcomplex is composed of METTL3 and METTL14 (PubMed:27602518). The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B) (PubMed:27602518). May interact with NCOR2 (PubMed:16129689). Interacts with NXF1, the interaction is required to promote mRNA export (PubMed:19586903).3 Publications
(Microbial infection) Interacts (via the SPOC domain) with Epstein-Barr virus BMLF1 (via the N-terminus); the interaction is direct.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi118943, 33 interactors
IntActiQ8NDT2, 13 interactors
STRINGi9606.ENSP00000313890

Structurei

3D structure databases

ProteinModelPortaliQ8NDT2
SMRiQ8NDT2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 219RRM 1PROSITE-ProRule annotationAdd BLAST81
Domaini337 – 414RRM 2PROSITE-ProRule annotationAdd BLAST78
Domaini418 – 492RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini711 – 889SPOCPROSITE-ProRule annotationAdd BLAST179

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni722 – 890Interaction with Epstein-Barr virus BMLF1Add BLAST169

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi593 – 597Nuclear localization signalSequence analysis5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi658 – 677His-richAdd BLAST20

Sequence similaritiesi

Belongs to the RRM Spen family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0112 Eukaryota
ENOG410XSAC LUCA
GeneTreeiENSGT00530000063730
HOGENOMiHOG000006547
HOVERGENiHBG058366
InParanoidiQ8NDT2
KOiK13190
OMAiCEFSQQY
OrthoDBiEOG091G0615
PhylomeDBiQ8NDT2
TreeFamiTF354224

Family and domain databases

CDDicd12554 RRM1_RBM15B, 1 hit
cd12556 RRM2_RBM15B, 1 hit
cd12558 RRM3_RBM15B, 1 hit
Gene3Di2.40.290.10, 1 hit
3.30.70.330, 3 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR034475 RBM15B_RRM1
IPR034535 RBM15B_RRM2
IPR034536 RBM15B_RRM3
IPR000504 RRM_dom
IPR016194 SPOC-like_C_dom_sf
IPR012921 SPOC_C
IPR010912 SPOC_met
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
PF07744 SPOC, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 3 hits
SUPFAMiSSF100939 SSF100939, 1 hit
SSF54928 SSF54928, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 3 hits
PS50917 SPOC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NDT2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKRQSERDSS PSGRGSSSSA KRPREREREA EAGGRRAAHK ASGGAKHPVP
60 70 80 90 100
ARARDKPRGS GSGGGGHRDG RGTGDANHRA SSGRSSGSGA GGGGRGGKAS
110 120 130 140 150
GDPGASGMSP RASPLPPPPP PPGAEPACPG SSAAAPEYKT LLISSLSPAL
160 170 180 190 200
PAEHLEDRLF HQFKRFGEIS LRLSHTPELG RVAYVNFRHP QDAREARQHA
210 220 230 240 250
LARQLLLYDR PLKVEPVYLR GGGGSSRRSS SSSAAASTPP PGPPAPADPL
260 270 280 290 300
GYLPLHGGYQ YKQRSLSPVA APPLREPRAR HAAAAFALDA AAAAAVGLSR
310 320 330 340 350
ERALDYYGLY DDRGRPYGYP AVCEEDLMPE DDQRATRNLF IGNLDHSVSE
360 370 380 390 400
VELRRAFEKY GIIEEVVIKR PARGQGGAYA FLKFQNLDMA HRAKVAMSGR
410 420 430 440 450
VIGRNPIKIG YGKANPTTRL WVGGLGPNTS LAALAREFDR FGSIRTIDHV
460 470 480 490 500
KGDSFAYIQY ESLDAAQAAC AKMRGFPLGG PDRRLRVDFA KAEETRYPQQ
510 520 530 540 550
YQPSPLPVHY ELLTDGYTRH RNLDADLVRD RTPPHLLYSD RDRTFLEGDW
560 570 580 590 600
TSPSKSSDRR NSLEGYSRSV RSRSGERWGA DGDRGLPKPW EERRKRRSLS
610 620 630 640 650
SDRGRTTHSP YEERSRTKGS GQQSERGSDR TPERSRKENH SSEGTKESSS
660 670 680 690 700
NSLSNSRHGA EERGHHHHHH EAADSSHGKK ARDSERNHRT TEAEPKPLEE
710 720 730 740 750
PKHETKKLKN LSEYAQTLQL GWNGLLVLKN SCFPTSMHIL EGDQGVISSL
760 770 780 790 800
LKDHTSGSKL TQLKIAQRLR LDQPKLDEVT RRIKQGSPNG YAVLLATQAT
810 820 830 840 850
PSGLGTEGMP TVEPGLQRRL LRNLVSYLKQ KQAAGVISLP VGGSKGRDGT
860 870 880 890
GMLYAFPPCD FSQQYLQSAL RTLGKLEEEH MVIVIVRDTA
Length:890
Mass (Da):97,205
Last modified:May 5, 2009 - v3
Checksum:i276C94252F036C83
GO
Isoform 2 (identifier: Q8NDT2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-327: Missing.

Note: No experimental confirmation available.
Show »
Length:563
Mass (Da):63,156
Checksum:i310F8918227B576B
GO

Sequence cautioni

The sequence AAH01367 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAS50153 differs from that shown. Contaminating sequence. Sequence of unknown origin in the C-terminal part.Curated
The sequence CAD38547 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti593R → K in AAI39837 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0538781 – 327Missing in isoform 2. 1 PublicationAdd BLAST327

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC092037 Genomic DNA No translation available.
BC001367 mRNA Translation: AAH01367.2 Different initiation.
BC139836 mRNA Translation: AAI39837.1
AL831838 mRNA Translation: CAD38547.2 Different initiation.
AY545557 mRNA Translation: AAS50153.1 Sequence problems.
CCDSiCCDS33764.1 [Q8NDT2-1]
RefSeqiNP_037418.3, NM_013286.4 [Q8NDT2-1]
UniGeneiHs.118738
Hs.716623
Hs.731737

Genome annotation databases

EnsembliENST00000563281; ENSP00000454545; ENSG00000259956 [Q8NDT2-1]
GeneIDi29890
KEGGihsa:29890
UCSCiuc003dbd.4 human [Q8NDT2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRB15B_HUMAN
AccessioniPrimary (citable) accession number: Q8NDT2
Secondary accession number(s): A4QPG7, Q6QE19, Q9BV96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: May 5, 2009
Last modified: July 18, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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