UniProtKB - Q8NDL9 (CBPC5_HUMAN)
Protein
Cytosolic carboxypeptidase-like protein 5
Gene
AGBL5
Organism
Homo sapiens (Human)
Status
Functioni
Metallocarboxypeptidase that mediates protein deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post-translational glutamylation in proteins such as tubulins. In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/CCP1, AGBL2/CCP2, AGBL3/CCP3, AGBL1/CCP4 and AGBL4/CCP6. Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS.By similarity
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 252 | ZincBy similarity | 1 | |
Metal bindingi | 255 | ZincBy similarity | 1 | |
Active sitei | 303 | NucleophileBy similarity | 1 | |
Metal bindingi | 434 | ZincBy similarity | 1 |
GO - Molecular functioni
- metallocarboxypeptidase activity Source: UniProtKB
- tubulin binding Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- defense response to virus Source: UniProtKB
- protein branching point deglutamylation Source: UniProtKB
- protein deglutamylation Source: UniProtKB
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8NDL9 |
Reactomei | R-HSA-8955332, Carboxyterminal post-translational modifications of tubulin |
Protein family/group databases
MEROPSi | M14.025 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic carboxypeptidase-like protein 5 (EC:3.4.17.-)Alternative name(s): ATP/GTP-binding protein-like 5 |
Gene namesi | Name:AGBL5 Synonyms:CCP5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000084693.15 |
HGNCi | HGNC:26147, AGBL5 |
MIMi | 615900, gene |
neXtProti | NX_Q8NDL9 |
Subcellular locationi
Cytosol
- cytosol By similarity
Nucleus
- Nucleus 1 Publication
Cytoskeleton
- spindle 1 Publication
Other locations
- Midbody 1 Publication
Note: Mainly cytoplasmic. Slight accumulation in the nucleus is observed (By similarity). Colocalizes with alpha-tubulin in the mitotic spindle and with midbody microtubules in the intercellular bridges formed during cytokinesis.By similarity1 Publication
Cytoskeleton
- microtubule cytoskeleton Source: HPA
- mitotic spindle Source: UniProtKB
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- intercellular bridge Source: HPA
Nucleus
- nucleus Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Involvement in diseasei
Retinitis pigmentosa 75 (RP75)4 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of retinitis pigmentosa, a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP75 inheritance is autosomal recessive.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_079522 | 108 | P → R in RP75; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_079523 | 251 | V → G in RP75; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_077018 | 276 | R → W in RP75. 1 PublicationCorresponds to variant dbSNP:rs879253769EnsemblClinVar. | 1 | |
Natural variantiVAR_079524 | 281 | R → C in RP75; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs780394281Ensembl. | 1 | |
Natural variantiVAR_077019 | 295 | D → N in RP75. 1 PublicationCorresponds to variant dbSNP:rs879253768EnsemblClinVar. | 1 | |
Natural variantiVAR_079525 | 487 – 886 | Missing in RP75. 1 PublicationAdd BLAST | 400 | |
Natural variantiVAR_079526 | 592 – 886 | Missing in RP75. 1 PublicationAdd BLAST | 295 |
Keywords - Diseasei
Disease mutation, Retinitis pigmentosaOrganism-specific databases
DisGeNETi | 60509 |
MalaCardsi | AGBL5 |
MIMi | 617023, phenotype |
OpenTargetsi | ENSG00000084693 |
Orphaneti | 791, Retinitis pigmentosa |
PharmGKBi | PA162375816 |
Miscellaneous databases
Pharosi | Q8NDL9, Tbio |
Polymorphism and mutation databases
BioMutai | AGBL5 |
DMDMi | 74715354 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000305921 | 1 – 886 | Cytosolic carboxypeptidase-like protein 5Add BLAST | 886 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 841 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8NDL9 |
jPOSTi | Q8NDL9 |
MassIVEi | Q8NDL9 |
MaxQBi | Q8NDL9 |
PaxDbi | Q8NDL9 |
PeptideAtlasi | Q8NDL9 |
PRIDEi | Q8NDL9 |
ProteomicsDBi | 73036 [Q8NDL9-1] 73037 [Q8NDL9-2] 73038 [Q8NDL9-3] |
PTM databases
iPTMneti | Q8NDL9 |
PhosphoSitePlusi | Q8NDL9 |
Expressioni
Tissue specificityi
Expressed in brain.
Gene expression databases
Bgeei | ENSG00000084693, Expressed in testis and 215 other tissues |
ExpressionAtlasi | Q8NDL9, baseline and differential |
Genevisiblei | Q8NDL9, HS |
Organism-specific databases
HPAi | ENSG00000084693, Tissue enriched (testis) |
Interactioni
GO - Molecular functioni
- tubulin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 121935, 6 interactors |
IntActi | Q8NDL9, 6 interactors |
MINTi | Q8NDL9 |
STRINGi | 9606.ENSP00000353249 |
Miscellaneous databases
RNActi | Q8NDL9, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 540 – 544 | Poly-Pro | 5 | |
Compositional biasi | 651 – 654 | Poly-Ser | 4 |
Sequence similaritiesi
Belongs to the peptidase M14 family.Curated
Phylogenomic databases
eggNOGi | KOG3641, Eukaryota |
GeneTreei | ENSGT00940000158032 |
HOGENOMi | CLU_007523_3_2_1 |
InParanoidi | Q8NDL9 |
OMAi | WFHFSVR |
OrthoDBi | 481670at2759 |
PhylomeDBi | Q8NDL9 |
TreeFami | TF324301 |
Family and domain databases
InterProi | View protein in InterPro IPR040626, Pepdidase_M14_N IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF18027, Pepdidase_M14_N, 1 hit PF00246, Peptidase_M14, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NDL9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELRCGGLLF SSRFDSGNLA HVEKVESLSS DGEGVGGGAS ALTSGIASSP
60 70 80 90 100
DYEFNVWTRP DCAETEFENG NRSWFYFSVR GGMPGKLIKI NIMNMNKQSK
110 120 130 140 150
LYSQGMAPFV RTLPTRPRWE RIRDRPTFEM TETQFVLSFV HRFVEGRGAT
160 170 180 190 200
TFFAFCYPFS YSDCQELLNQ LDQRFPENHP THSSPLDTIY YHRELLCYSL
210 220 230 240 250
DGLRVDLLTI TSCHGLREDR EPRLEQLFPD TSTPRPFRFA GKRIFFLSSR
260 270 280 290 300
VHPGETPSSF VFNGFLDFIL RPDDPRAQTL RRLFVFKLIP MLNPDGVVRG
310 320 330 340 350
HYRTDSRGVN LNRQYLKPDA VLHPAIYGAK AVLLYHHVHS RLNSQSSSEH
360 370 380 390 400
QPSSCLPPDA PVSDLEKANN LQNEAQCGHS ADRHNAEAWK QTEPAEQKLN
410 420 430 440 450
SVWIMPQQSA GLEESAPDTI PPKESGVAYY VDLHGHASKR GCFMYGNSFS
460 470 480 490 500
DESTQVENML YPKLISLNSA HFDFQGCNFS EKNMYARDRR DGQSKEGSGR
510 520 530 540 550
VAIYKASGII HSYTLECNYN TGRSVNSIPA ACHDNGRASP PPPPAFPSRY
560 570 580 590 600
TVELFEQVGR AMAIAALDMA ECNPWPRIVL SEHSSLTNLR AWMLKHVRNS
610 620 630 640 650
RGLSSTLNVG VNKKRGLRTP PKSHNGLPVS CSENTLSRAR SFSTGTSAGG
660 670 680 690 700
SSSSQQNSPQ MKNSPSFPFH GSRPAGLPGL GSSTQKVTHR VLGPVREPRS
710 720 730 740 750
QDRRRQQQPL NHRPAGSLAP SPAPTSSGPA SSHKLGSCLL PDSFNIPGSS
760 770 780 790 800
CSLLSSGDKP EAVMVIGKGL LGTGARMPCI KTRLQARPRL GRGSPPTRRG
810 820 830 840 850
MKGSSGPTSP TPRTRESSEL ELGSCSATPG LPQARPPRPR SAPAFSPISC
860 870 880
SLSDSPSWNC YSRGPLGQPE VCFVPKSPPL TVSPRV
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH7C1L5 | H7C1L5_HUMAN | Cytosolic carboxypeptidase-like pro... | AGBL5 | 137 | Annotation score: | ||
C9JQG9 | C9JQG9_HUMAN | Cytosolic carboxypeptidase-like pro... | AGBL5 | 157 | Annotation score: | ||
C9JCE1 | C9JCE1_HUMAN | Cytosolic carboxypeptidase-like pro... | AGBL5 | 65 | Annotation score: | ||
C9JHM6 | C9JHM6_HUMAN | Cytosolic carboxypeptidase-like pro... | AGBL5 | 25 | Annotation score: | ||
C9JTY1 | C9JTY1_HUMAN | Cytosolic carboxypeptidase-like pro... | AGBL5 | 66 | Annotation score: |
Sequence cautioni
The sequence AAX93164 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAY14655 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAB15151 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 38 – 40 | GAS → CLL in AAI31499 (PubMed:15489334).Curated | 3 | |
Sequence conflicti | 94 | N → S in BAH13623 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 122 | I → R in AAI31499 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 672 | S → G in BAB15151 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_079522 | 108 | P → R in RP75; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_079523 | 251 | V → G in RP75; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_077018 | 276 | R → W in RP75. 1 PublicationCorresponds to variant dbSNP:rs879253769EnsemblClinVar. | 1 | |
Natural variantiVAR_079524 | 281 | R → C in RP75; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs780394281Ensembl. | 1 | |
Natural variantiVAR_077019 | 295 | D → N in RP75. 1 PublicationCorresponds to variant dbSNP:rs879253768EnsemblClinVar. | 1 | |
Natural variantiVAR_079525 | 487 – 886 | Missing in RP75. 1 PublicationAdd BLAST | 400 | |
Natural variantiVAR_079526 | 592 – 886 | Missing in RP75. 1 PublicationAdd BLAST | 295 | |
Natural variantiVAR_035231 | 649 | G → D. Corresponds to variant dbSNP:rs35804461EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_028359 | 697 – 717 | EPRSQ…RPAGS → GKPVWEPLQHVFGCLGHCWG K in isoform 3. 2 PublicationsAdd BLAST | 21 | |
Alternative sequenceiVSP_028360 | 718 – 886 | Missing in isoform 3. 2 PublicationsAdd BLAST | 169 | |
Alternative sequenceiVSP_028361 | 786 – 816 | ARPRL…PRTRE → TCPRRVSARRGPGFPRLGPG WAGAHRRLAEG in isoform 2. 1 PublicationAdd BLAST | 31 | |
Alternative sequenceiVSP_028362 | 817 – 886 | Missing in isoform 2. 1 PublicationAdd BLAST | 70 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL833844 mRNA Translation: CAD38704.1 AK302091 mRNA Translation: BAH13623.1 AC013403 Genomic DNA Translation: AAX93164.1 Sequence problems. AC013472 Genomic DNA Translation: AAY14655.1 Sequence problems. CH471053 Genomic DNA Translation: EAX00649.1 CH471053 Genomic DNA Translation: EAX00651.1 CH471053 Genomic DNA Translation: EAX00652.1 CH471053 Genomic DNA Translation: EAX00653.1 BC007415 mRNA Translation: AAH07415.2 BC018584 mRNA No translation available. BC131498 mRNA Translation: AAI31499.1 AK023398 mRNA Translation: BAB14560.1 AK025492 mRNA Translation: BAB15151.1 Different initiation. |
CCDSi | CCDS1732.3 [Q8NDL9-1] CCDS42665.1 [Q8NDL9-3] |
RefSeqi | NP_001030584.1, NM_001035507.2 [Q8NDL9-3] NP_068603.4, NM_021831.5 [Q8NDL9-1] XP_011531313.1, XM_011533011.2 [Q8NDL9-1] XP_011531314.1, XM_011533012.2 [Q8NDL9-1] XP_011531315.1, XM_011533013.2 [Q8NDL9-1] |
Genome annotation databases
Ensembli | ENST00000323064; ENSP00000323681; ENSG00000084693 [Q8NDL9-3] ENST00000360131; ENSP00000353249; ENSG00000084693 [Q8NDL9-1] ENST00000487078; ENSP00000433830; ENSG00000084693 [Q8NDL9-2] |
GeneIDi | 60509 |
KEGGi | hsa:60509 |
UCSCi | uc002rid.4, human [Q8NDL9-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL833844 mRNA Translation: CAD38704.1 AK302091 mRNA Translation: BAH13623.1 AC013403 Genomic DNA Translation: AAX93164.1 Sequence problems. AC013472 Genomic DNA Translation: AAY14655.1 Sequence problems. CH471053 Genomic DNA Translation: EAX00649.1 CH471053 Genomic DNA Translation: EAX00651.1 CH471053 Genomic DNA Translation: EAX00652.1 CH471053 Genomic DNA Translation: EAX00653.1 BC007415 mRNA Translation: AAH07415.2 BC018584 mRNA No translation available. BC131498 mRNA Translation: AAI31499.1 AK023398 mRNA Translation: BAB14560.1 AK025492 mRNA Translation: BAB15151.1 Different initiation. |
CCDSi | CCDS1732.3 [Q8NDL9-1] CCDS42665.1 [Q8NDL9-3] |
RefSeqi | NP_001030584.1, NM_001035507.2 [Q8NDL9-3] NP_068603.4, NM_021831.5 [Q8NDL9-1] XP_011531313.1, XM_011533011.2 [Q8NDL9-1] XP_011531314.1, XM_011533012.2 [Q8NDL9-1] XP_011531315.1, XM_011533013.2 [Q8NDL9-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 121935, 6 interactors |
IntActi | Q8NDL9, 6 interactors |
MINTi | Q8NDL9 |
STRINGi | 9606.ENSP00000353249 |
Protein family/group databases
MEROPSi | M14.025 |
PTM databases
iPTMneti | Q8NDL9 |
PhosphoSitePlusi | Q8NDL9 |
Polymorphism and mutation databases
BioMutai | AGBL5 |
DMDMi | 74715354 |
Proteomic databases
EPDi | Q8NDL9 |
jPOSTi | Q8NDL9 |
MassIVEi | Q8NDL9 |
MaxQBi | Q8NDL9 |
PaxDbi | Q8NDL9 |
PeptideAtlasi | Q8NDL9 |
PRIDEi | Q8NDL9 |
ProteomicsDBi | 73036 [Q8NDL9-1] 73037 [Q8NDL9-2] 73038 [Q8NDL9-3] |
Protocols and materials databases
Antibodypediai | 28106, 149 antibodies |
DNASUi | 60509 |
Genome annotation databases
Ensembli | ENST00000323064; ENSP00000323681; ENSG00000084693 [Q8NDL9-3] ENST00000360131; ENSP00000353249; ENSG00000084693 [Q8NDL9-1] ENST00000487078; ENSP00000433830; ENSG00000084693 [Q8NDL9-2] |
GeneIDi | 60509 |
KEGGi | hsa:60509 |
UCSCi | uc002rid.4, human [Q8NDL9-1] |
Organism-specific databases
CTDi | 60509 |
DisGeNETi | 60509 |
EuPathDBi | HostDB:ENSG00000084693.15 |
GeneCardsi | AGBL5 |
HGNCi | HGNC:26147, AGBL5 |
HPAi | ENSG00000084693, Tissue enriched (testis) |
MalaCardsi | AGBL5 |
MIMi | 615900, gene 617023, phenotype |
neXtProti | NX_Q8NDL9 |
OpenTargetsi | ENSG00000084693 |
Orphaneti | 791, Retinitis pigmentosa |
PharmGKBi | PA162375816 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3641, Eukaryota |
GeneTreei | ENSGT00940000158032 |
HOGENOMi | CLU_007523_3_2_1 |
InParanoidi | Q8NDL9 |
OMAi | WFHFSVR |
OrthoDBi | 481670at2759 |
PhylomeDBi | Q8NDL9 |
TreeFami | TF324301 |
Enzyme and pathway databases
PathwayCommonsi | Q8NDL9 |
Reactomei | R-HSA-8955332, Carboxyterminal post-translational modifications of tubulin |
Miscellaneous databases
BioGRID-ORCSi | 60509, 9 hits in 846 CRISPR screens |
ChiTaRSi | AGBL5, human |
GenomeRNAii | 60509 |
Pharosi | Q8NDL9, Tbio |
PROi | PR:Q8NDL9 |
RNActi | Q8NDL9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000084693, Expressed in testis and 215 other tissues |
ExpressionAtlasi | Q8NDL9, baseline and differential |
Genevisiblei | Q8NDL9, HS |
Family and domain databases
InterProi | View protein in InterPro IPR040626, Pepdidase_M14_N IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF18027, Pepdidase_M14_N, 1 hit PF00246, Peptidase_M14, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CBPC5_HUMAN | |
Accessioni | Q8NDL9Primary (citable) accession number: Q8NDL9 Secondary accession number(s): A2VDI7 Q9H8P8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Peptidase families
Classification of peptidase families and list of entries