UniProtKB - Q8NDF8 (PAPD5_HUMAN)
Terminal nucleotidyltransferase 4B
TENT4B
Functioni
Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation (PubMed:21788334, PubMed:30026317).
Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro) (PubMed:21788334, PubMed:21855801).
Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1 (PubMed:28383716).
Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion (PubMed:18172165).
Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation (PubMed:25049417).
Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation (PubMed:22442037).
7 PublicationsCaution
Catalytic activityi
- EC:2.7.7.191 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 177 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 179 | Magnesium; catalyticBy similarity | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
- guanylyltransferase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- polynucleotide adenylyltransferase activity Source: UniProtKB
- RNA binding Source: UniProtKB
- telomerase RNA binding Source: BHF-UCL
GO - Biological processi
- carbohydrate homeostasis Source: UniProtKB
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- histone mRNA catabolic process Source: UniProtKB
- miRNA catabolic process Source: UniProtKB
- mRNA polyadenylation Source: UniProtKB
- ncRNA polyadenylation Source: UniProtKB
- ncRNA polyadenylation involved in polyadenylation-dependent ncRNA catabolic process Source: BHF-UCL
- negative regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
- negative regulation of telomere maintenance via telomerase Source: BHF-UCL
- positive regulation of 3'-UTR-mediated mRNA stabilization Source: UniProtKB
- RNA 3'-end processing Source: GO_Central
- RNA 3' uridylation Source: UniProtKB
- rRNA processing Source: UniProtKB-KW
- sno(s)RNA polyadenylation Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase |
Biological process | Cell cycle, Cell division, Mitosis, mRNA processing, rRNA processing |
Ligand | Magnesium, Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.7.7.19, 2681 |
PathwayCommonsi | Q8NDF8 |
SignaLinki | Q8NDF8 |
Names & Taxonomyi
Protein namesi | Recommended name: Terminal nucleotidyltransferase 4BCuratedAlternative name(s): Non-canonical poly(A) RNA polymerase PAPD5Curated (EC:2.7.7.191 Publication) PAP-associated domain-containing protein 5 Terminal guanylyltransferaseCurated (EC:2.7.7.-1 Publication) Terminal uridylyltransferase 3 Short name: TUTase 3 Topoisomerase-related function protein 4-2 Short name: TRF4-21 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30758, TENT4B |
MIMi | 605540, gene |
neXtProti | NX_Q8NDF8 |
VEuPathDBi | HostDB:ENSG00000121274 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleolus Source: UniProtKB
- TRAMP complex Source: UniProtKB
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 64282 |
OpenTargetsi | ENSG00000121274 |
PharmGKBi | PA134949693 |
Miscellaneous databases
Pharosi | Q8NDF8, Tbio |
Genetic variation databases
BioMutai | PAPD5 |
DMDMi | 59800139 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120310 | 1 – 572 | Terminal nucleotidyltransferase 4BAdd BLAST | 572 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Cross-linki | 470 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Modified residuei | 484 | PhosphoserineCombined sources | 1 | ||
Cross-linki | 497 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 512 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Cross-linki | 526 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Isoform 5 (identifier: Q8NDF8-5) | |||||
Cross-linki | 151 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Curated |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8NDF8 |
jPOSTi | Q8NDF8 |
MassIVEi | Q8NDF8 |
MaxQBi | Q8NDF8 |
PaxDbi | Q8NDF8 |
PeptideAtlasi | Q8NDF8 |
PRIDEi | Q8NDF8 |
ProteomicsDBi | 5240 73020 [Q8NDF8-1] 73021 [Q8NDF8-2] 73022 [Q8NDF8-3] 73023 [Q8NDF8-4] |
PTM databases
GlyGeni | Q8NDF8, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q8NDF8 |
PhosphoSitePlusi | Q8NDF8 |
Expressioni
Gene expression databases
Bgeei | ENSG00000121274, Expressed in secondary oocyte and 224 other tissues |
ExpressionAtlasi | Q8NDF8, baseline and differential |
Genevisiblei | Q8NDF8, HS |
Organism-specific databases
HPAi | ENSG00000121274, Low tissue specificity |
Interactioni
Subunit structurei
Component of a nucleolar TRAMP-like complex, an ATP-dependent exosome regulatory complex consisting of a helicase (MTREX), an oligadenylate polymerase (TENT4B or TENT4A), and a substrate specific RNA-binding factor (ZCCHC7 or ZCCHC8). Several TRAMP-like complexes exist with specific compositions and are associated with nuclear, or nucleolar RNA exosomes (PubMed:21855801).
Interacts with CPEB1; the interaction is required for TENT4B-mediated translational control (PubMed:28383716).
2 PublicationsProtein-protein interaction databases
BioGRIDi | 122126, 170 interactors |
DIPi | DIP-59183N |
ELMi | Q8NDF8 |
IntActi | Q8NDF8, 18 interactors |
MINTi | Q8NDF8 |
Miscellaneous databases
RNActi | Q8NDF8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 308 – 368 | PAP-associatedAdd BLAST | 61 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 105 | DisorderedSequence analysisAdd BLAST | 105 | |
Regioni | 435 – 572 | DisorderedSequence analysisAdd BLAST | 138 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 557 – 563 | Basic, involved in binding of the RNA primer | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 22 – 36 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 45 – 59 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 441 – 464 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 471 – 551 | Polar residuesSequence analysisAdd BLAST | 81 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1906, Eukaryota |
GeneTreei | ENSGT00940000158301 |
HOGENOMi | CLU_013572_3_2_1 |
InParanoidi | Q8NDF8 |
OrthoDBi | 1016712at2759 |
PhylomeDBi | Q8NDF8 |
TreeFami | TF313939 |
Family and domain databases
Gene3Di | 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR043519, NT_sf IPR002058, PAP_assoc IPR002934, Polymerase_NTP_transf_dom IPR040136, TENT4B IPR045862, Trf4-like |
PANTHERi | PTHR23092, PTHR23092, 2 hits PTHR23092:SF51, PTHR23092:SF51, 2 hits |
Pfami | View protein in Pfam PF01909, NTP_transf_2, 1 hit PF03828, PAP_assoc, 1 hit |
SUPFAMi | SSF81301, SSF81301, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MYRSGERLLG SHALPAEQRD FLPLETTNNN NNHHQPGAWA RRAGSSASSP
60 70 80 90 100
PSASSSPHPS AAVPAADPAD SASGSSNKRK RDNKASGGRA AGGGRADGGG
110 120 130 140 150
VVYSGTPWKR RNYNQGVVGL HEEISDFYEY MSPRPEEEKM RMEVVNRIES
160 170 180 190 200
VIKELWPSAD VQIFGSFKTG LYLPTSDIDL VVFGKWENLP LWTLEEALRK
210 220 230 240 250
HKVADEDSVK VLDKATVPII KLTDSFTEVK VDISFNVQNG VRAADLIKDF
260 270 280 290 300
TKKYPVLPYL VLVLKQFLLQ RDLNEVFTGG IGSYSLFLMA VSFLQLHPRE
310 320 330 340 350
DACIPNTNYG VLLIEFFELY GRHFNYLKTG IRIKDGGSYV AKDEVQKNML
360 370 380 390 400
DGYRPSMLYI EDPLQPGNDV GRSSYGAMQV KQAFDYAYVV LSHAVSPIAK
410 420 430 440 450
YYPNNETESI LGRIIRVTDE VATYRDWISK QWGLKNRPEP SCNGPVSSSS
460 470 480 490 500
ATQSSSSDVD SDATPCKTPK QLLCRPSTGN RVGSQDVSLE SSQAVGKMQS
510 520 530 540 550
TQTTNTSNST NKSQHGSARL FRSSSKGFQG TTQTSHGSLM TNKQHQGKSN
560 570
NQYYHGKKRK HKRDAPLSDL CR
The sequence of this isoform differs from the canonical sequence as follows:
1-1: M → MRPRPRSAPGKPRRRSRARLRSSRTPSGGASGGGGSSSSSSTATGGSGSSTGSPGGAASAPAPAPAGM
85-85: A → ASTYGLNYSLLQP
444-444: G → GNGVTLIVDTQQLDKCNNNLSEENEALGKCRSKTSESLSKHSSNSSSG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A7N4YH79 | A0A7N4YH79_HUMAN | Terminal nucleotidyltransferase 4B | TENT4B | 713 | Annotation score: | ||
E9PC61 | E9PC61_HUMAN | Terminal nucleotidyltransferase 4B | TENT4B | 303 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046989 | 1 | M → MRPRPRSAPGKPRRRSRARL RSSRTPSGGASGGGGSSSSS STATGGSGSSTGSPGGAASA PAPAPAGM in isoform 5. 1 Publication | 1 | |
Alternative sequenceiVSP_046990 | 85 | A → ASTYGLNYSLLQP in isoform 5. 1 Publication | 1 | |
Alternative sequenceiVSP_012732 | 368 – 370 | NDV → IEI in isoform 4. 1 Publication | 3 | |
Alternative sequenceiVSP_012733 | 371 – 572 | Missing in isoform 4. 1 PublicationAdd BLAST | 202 | |
Alternative sequenceiVSP_012735 | 444 | G → GNGVTLIVDTQQLDKCNNNL SEENEALGKCRSKTSESLSK HSSNSSSG in isoform 3 and isoform 5. 2 Publications | 1 | |
Alternative sequenceiVSP_012734 | 445 – 465 | PVSSS…SDATP → NETLHQVQCRPLLPHSPALV T in isoform 2. 1 PublicationAdd BLAST | 21 | |
Alternative sequenceiVSP_012736 | 466 – 572 | Missing in isoform 2. 1 PublicationAdd BLAST | 107 | |
Alternative sequenceiVSP_012737 | 539 – 572 | Missing in isoform 3. 1 PublicationAdd BLAST | 34 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK001141 mRNA Translation: BAA91518.1 Different initiation. AK097589 mRNA No translation available. AK300918 mRNA Translation: BAG62550.1 AC007597 Genomic DNA No translation available. AC007610 Genomic DNA No translation available. AF089897 mRNA Translation: AAD45199.1 Different initiation. AL833922 mRNA Translation: CAD38778.1 |
CCDSi | CCDS54006.1 [Q8NDF8-5] |
RefSeqi | NP_001035374.2, NM_001040284.2 [Q8NDF8-5] NP_001035375.2, NM_001040285.2 |
Genome annotation databases
Ensembli | ENST00000436909.8; ENSP00000396995.3; ENSG00000121274.14 [Q8NDF8-5] |
GeneIDi | 64282 |
KEGGi | hsa:64282 |
UCSCi | uc010vgo.3, human [Q8NDF8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK001141 mRNA Translation: BAA91518.1 Different initiation. AK097589 mRNA No translation available. AK300918 mRNA Translation: BAG62550.1 AC007597 Genomic DNA No translation available. AC007610 Genomic DNA No translation available. AF089897 mRNA Translation: AAD45199.1 Different initiation. AL833922 mRNA Translation: CAD38778.1 |
CCDSi | CCDS54006.1 [Q8NDF8-5] |
RefSeqi | NP_001035374.2, NM_001040284.2 [Q8NDF8-5] NP_001035375.2, NM_001040285.2 |
3D structure databases
AlphaFoldDBi | Q8NDF8 |
SMRi | Q8NDF8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122126, 170 interactors |
DIPi | DIP-59183N |
ELMi | Q8NDF8 |
IntActi | Q8NDF8, 18 interactors |
MINTi | Q8NDF8 |
PTM databases
GlyGeni | Q8NDF8, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q8NDF8 |
PhosphoSitePlusi | Q8NDF8 |
Genetic variation databases
BioMutai | PAPD5 |
DMDMi | 59800139 |
Proteomic databases
EPDi | Q8NDF8 |
jPOSTi | Q8NDF8 |
MassIVEi | Q8NDF8 |
MaxQBi | Q8NDF8 |
PaxDbi | Q8NDF8 |
PeptideAtlasi | Q8NDF8 |
PRIDEi | Q8NDF8 |
ProteomicsDBi | 5240 73020 [Q8NDF8-1] 73021 [Q8NDF8-2] 73022 [Q8NDF8-3] 73023 [Q8NDF8-4] |
Protocols and materials databases
Antibodypediai | 28218, 150 antibodies from 26 providers |
DNASUi | 64282 |
Genome annotation databases
Ensembli | ENST00000436909.8; ENSP00000396995.3; ENSG00000121274.14 [Q8NDF8-5] |
GeneIDi | 64282 |
KEGGi | hsa:64282 |
UCSCi | uc010vgo.3, human [Q8NDF8-1] |
Organism-specific databases
CTDi | 64282 |
DisGeNETi | 64282 |
GeneCardsi | TENT4B |
HGNCi | HGNC:30758, TENT4B |
HPAi | ENSG00000121274, Low tissue specificity |
MIMi | 605540, gene |
neXtProti | NX_Q8NDF8 |
OpenTargetsi | ENSG00000121274 |
PharmGKBi | PA134949693 |
VEuPathDBi | HostDB:ENSG00000121274 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1906, Eukaryota |
GeneTreei | ENSGT00940000158301 |
HOGENOMi | CLU_013572_3_2_1 |
InParanoidi | Q8NDF8 |
OrthoDBi | 1016712at2759 |
PhylomeDBi | Q8NDF8 |
TreeFami | TF313939 |
Enzyme and pathway databases
BRENDAi | 2.7.7.19, 2681 |
PathwayCommonsi | Q8NDF8 |
SignaLinki | Q8NDF8 |
Miscellaneous databases
BioGRID-ORCSi | 64282, 58 hits in 1083 CRISPR screens |
ChiTaRSi | PAPD5, human |
GenomeRNAii | 64282 |
Pharosi | Q8NDF8, Tbio |
PROi | PR:Q8NDF8 |
RNActi | Q8NDF8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000121274, Expressed in secondary oocyte and 224 other tissues |
ExpressionAtlasi | Q8NDF8, baseline and differential |
Genevisiblei | Q8NDF8, HS |
Family and domain databases
Gene3Di | 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR043519, NT_sf IPR002058, PAP_assoc IPR002934, Polymerase_NTP_transf_dom IPR040136, TENT4B IPR045862, Trf4-like |
PANTHERi | PTHR23092, PTHR23092, 2 hits PTHR23092:SF51, PTHR23092:SF51, 2 hits |
Pfami | View protein in Pfam PF01909, NTP_transf_2, 1 hit PF03828, PAP_assoc, 1 hit |
SUPFAMi | SSF81301, SSF81301, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PAPD5_HUMAN | |
Accessioni | Q8NDF8Primary (citable) accession number: Q8NDF8 Secondary accession number(s): B4DV38, Q9NW67, Q9Y6C0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | February 1, 2005 | |
Last modified: | May 25, 2022 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families