UniProtKB - Q8NCW6 (GLT11_HUMAN)
Protein
Polypeptide N-acetylgalactosaminyltransferase 11
Gene
GALNT11
Organism
Homo sapiens (Human)
Status
Functioni
Polypeptide N-acetylgalactosaminyltransferase that catalyzes the initiation of protein O-linked glycosylation and is involved in left/right asymmetry by mediating O-glycosylation of NOTCH1. O-glycosylation of NOTCH1 promotes activation of NOTCH1, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). Polypeptide N-acetylgalactosaminyltransferases catalyze the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays the same enzyme activity toward MUC1, MUC4, and EA2 than GALNT1. Not involved in glycosylation of erythropoietin (EPO).2 Publications
Catalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDP1 PublicationEC:2.4.1.411 Publication
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDP1 PublicationEC:2.4.1.411 Publication
Cofactori
Mn2+By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 191 | SubstrateBy similarity | 1 | |
Binding sitei | 222 | SubstrateBy similarity | 1 | |
Metal bindingi | 245 | ManganeseBy similarity | 1 | |
Binding sitei | 246 | SubstrateBy similarity | 1 | |
Metal bindingi | 247 | ManganeseBy similarity | 1 | |
Binding sitei | 350 | SubstrateBy similarity | 1 | |
Metal bindingi | 378 | ManganeseBy similarity | 1 | |
Binding sitei | 381 | SubstrateBy similarity | 1 | |
Binding sitei | 386 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- Notch binding Source: UniProtKB
- polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB
GO - Biological processi
- cilium assembly Source: UniProtKB
- determination of left/right symmetry Source: UniProtKB
- Notch receptor processing Source: UniProtKB
- Notch signaling involved in heart development Source: UniProtKB
- O-glycan processing Source: Reactome
- protein O-linked glycosylation via threonine Source: UniProtKB
- regulation of Notch signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Notch signaling pathway |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.4.1.41, 2681 |
PathwayCommonsi | Q8NCW6 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
SABIO-RKi | Q8NCW6 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: Polypeptide N-acetylgalactosaminyltransferase 11 (EC:2.4.1.41)Alternative name(s): Polypeptide GalNAc transferase 11 Short name: GalNAc-T11 Short name: pp-GaNTase 11 Protein-UDP acetylgalactosaminyltransferase 11 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11 |
Gene namesi | Name:GALNT11 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000178234.12 |
HGNCi | HGNC:19875, GALNT11 |
MIMi | 615130, gene |
neXtProti | NX_Q8NCW6 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 6 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 7 – 29 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 30 – 608 | LumenalSequence analysisAdd BLAST | 579 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Involvement in diseasei
Defects in GALNT11 may be a cause of heterotaxy, a congenital heart disease resulting from abnormalities in left-right (LR) body patterning.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 247 | H → A: Abolishes glycosyltransferase activity and ability to rescue left-right patterning defects induced by galnt11 knockdown in X.tropicalis. 1 Publication | 1 |
Keywords - Diseasei
HeterotaxyOrganism-specific databases
DisGeNETi | 63917 |
OpenTargetsi | ENSG00000178234 |
PharmGKBi | PA134911149 |
Miscellaneous databases
Pharosi | Q8NCW6, Tbio |
Polymorphism and mutation databases
BioMutai | GALNT11 |
DMDMi | 51316030 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059125 | 1 – 608 | Polypeptide N-acetylgalactosaminyltransferase 11Add BLAST | 608 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 95 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 141 ↔ 373 | PROSITE-ProRule annotation | ||
Disulfide bondi | 364 ↔ 441 | PROSITE-ProRule annotation | ||
Glycosylationi | 428 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 493 ↔ 512 | PROSITE-ProRule annotation | ||
Disulfide bondi | 536 ↔ 553 | PROSITE-ProRule annotation | ||
Disulfide bondi | 578 ↔ 596 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q8NCW6 |
jPOSTi | Q8NCW6 |
MassIVEi | Q8NCW6 |
MaxQBi | Q8NCW6 |
PaxDbi | Q8NCW6 |
PeptideAtlasi | Q8NCW6 |
PRIDEi | Q8NCW6 |
ProteomicsDBi | 72960 [Q8NCW6-1] 72961 [Q8NCW6-2] |
PTM databases
GlyGeni | Q8NCW6, 2 sites, 1 O-linked glycan (1 site) |
iPTMneti | Q8NCW6 |
PhosphoSitePlusi | Q8NCW6 |
Expressioni
Tissue specificityi
Highly expressed in kidney. Expressed at intermediate level in brain, heart and skeletal muscle. Weakly expressed other tissues. In kidney, it is strongly expressed in tubules but not expressed in glomeruli.1 Publication
Gene expression databases
Bgeei | ENSG00000178234, Expressed in cortex of kidney and 249 other tissues |
ExpressionAtlasi | Q8NCW6, baseline and differential |
Genevisiblei | Q8NCW6, HS |
Organism-specific databases
HPAi | ENSG00000178234, Tissue enhanced (kidney) |
Interactioni
Subunit structurei
Interacts with NOTCH1.
1 PublicationBinary interactionsi
Hide detailsIsoform 2 [Q8NCW6-2]
With | #Exp. | IntAct |
---|---|---|
GSC2 [O15499] | 3 | EBI-13364322,EBI-19954058 |
SGTB [Q96EQ0] | 3 | EBI-13364322,EBI-744081 |
GO - Molecular functioni
- Notch binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 121988, 29 interactors |
IntActi | Q8NCW6, 16 interactors |
STRINGi | 9606.ENSP00000416787 |
Miscellaneous databases
RNActi | Q8NCW6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 476 – 607 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 132 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 150 – 261 | Catalytic subdomain AAdd BLAST | 112 | |
Regioni | 319 – 381 | Catalytic subdomain BAdd BLAST | 63 |
Domaini
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000158227 |
HOGENOMi | CLU_013477_0_1_1 |
InParanoidi | Q8NCW6 |
OMAi | CDGSLPQ |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q8NCW6 |
TreeFami | TF313267 |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NCW6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGSVTVRYFC YGCLFTSATW TVLLFVYFNF SEVTQPLKNV PVKGSGPHGP
60 70 80 90 100
SPKKFYPRFT RGPSRVLEPQ FKANKIDDVI DSRVEDPEEG HLKFSSELGM
110 120 130 140 150
IFNERDQELR DLGYQKHAFN MLISDRLGYH RDVPDTRNAA CKEKFYPPDL
160 170 180 190 200
PAASVVICFY NEAFSALLRT VHSVIDRTPA HLLHEIILVD DDSDFDDLKG
210 220 230 240 250
ELDEYVQKYL PGKIKVIRNT KREGLIRGRM IGAAHATGEV LVFLDSHCEV
260 270 280 290 300
NVMWLQPLLA AIREDRHTVV CPVIDIISAD TLAYSSSPVV RGGFNWGLHF
310 320 330 340 350
KWDLVPLSEL GRAEGATAPI KSPTMAGGLF AMNRQYFHEL GQYDSGMDIW
360 370 380 390 400
GGENLEISFR IWMCGGKLFI IPCSRVGHIF RKRRPYGSPE GQDTMTHNSL
410 420 430 440 450
RLAHVWLDEY KEQYFSLRPD LKTKSYGNIS ERVELRKKLG CKSFKWYLDN
460 470 480 490 500
VYPEMQISGS HAKPQQPIFV NRGPKRPKVL QRGRLYHLQT NKCLVAQGRP
510 520 530 540 550
SQKGGLVVLK ACDYSDPNQI WIYNEEHELV LNSLLCLDMS ETRSSDPPRL
560 570 580 590 600
MKCHGSGGSQ QWTFGKNNRL YQVSVGQCLR AVDPLGQKGS VAMAICDGSS
SQQWHLEG
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB7Z3L9 | B7Z3L9_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT11 | 133 | Annotation score: | ||
C9J8A7 | C9J8A7_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT11 | 186 | Annotation score: | ||
C9K0N9 | C9K0N9_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT11 | 151 | Annotation score: | ||
C9J111 | C9J111_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT11 | 160 | Annotation score: | ||
C9JMT8 | C9JMT8_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT11 | 98 | Annotation score: | ||
C9JPR8 | C9JPR8_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT11 | 113 | Annotation score: | ||
H7BZL5 | H7BZL5_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT11 | 65 | Annotation score: |
Sequence cautioni
The sequence BAB15338 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 94 | F → L in BAG54116 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 551 | M → V in BAB15105 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 577 | Q → L in BAB15105 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_019589 | 151 | P → S. Corresponds to variant dbSNP:rs6464201EnsemblClinVar. | 1 | |
Natural variantiVAR_019590 | 197 | D → Y1 PublicationCorresponds to variant dbSNP:rs3778922Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011215 | 196 – 202 | DDLKGEL → GKECCTW in isoform 2. 1 Publication | 7 | |
Alternative sequenceiVSP_011216 | 203 – 608 | Missing in isoform 2. 1 PublicationAdd BLAST | 406 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12434 mRNA Translation: CAC79625.3 AK025287 mRNA Translation: BAB15105.1 AK026056 mRNA Translation: BAB15338.1 Different initiation. AK124934 mRNA Translation: BAG54116.1 AC006017 Genomic DNA Translation: AAD45821.1 BC059377 mRNA Translation: AAH59377.1 |
CCDSi | CCDS5930.1 [Q8NCW6-1] |
RefSeqi | NP_071370.2, NM_022087.3 [Q8NCW6-1] XP_006716145.1, XM_006716082.2 XP_006716146.1, XM_006716083.2 [Q8NCW6-1] XP_006716147.1, XM_006716084.2 [Q8NCW6-1] XP_016867989.1, XM_017012500.1 |
Genome annotation databases
Ensembli | ENST00000415421; ENSP00000410093; ENSG00000178234 [Q8NCW6-2] ENST00000422997; ENSP00000389449; ENSG00000178234 [Q8NCW6-2] ENST00000430044; ENSP00000395122; ENSG00000178234 [Q8NCW6-1] ENST00000434507; ENSP00000416787; ENSG00000178234 [Q8NCW6-1] |
GeneIDi | 63917 |
KEGGi | hsa:63917 |
UCSCi | uc003wku.3, human [Q8NCW6-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Polypeptide N-acetylgalactosaminyltransferase 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12434 mRNA Translation: CAC79625.3 AK025287 mRNA Translation: BAB15105.1 AK026056 mRNA Translation: BAB15338.1 Different initiation. AK124934 mRNA Translation: BAG54116.1 AC006017 Genomic DNA Translation: AAD45821.1 BC059377 mRNA Translation: AAH59377.1 |
CCDSi | CCDS5930.1 [Q8NCW6-1] |
RefSeqi | NP_071370.2, NM_022087.3 [Q8NCW6-1] XP_006716145.1, XM_006716082.2 XP_006716146.1, XM_006716083.2 [Q8NCW6-1] XP_006716147.1, XM_006716084.2 [Q8NCW6-1] XP_016867989.1, XM_017012500.1 |
3D structure databases
SMRi | Q8NCW6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 121988, 29 interactors |
IntActi | Q8NCW6, 16 interactors |
STRINGi | 9606.ENSP00000416787 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
PTM databases
GlyGeni | Q8NCW6, 2 sites, 1 O-linked glycan (1 site) |
iPTMneti | Q8NCW6 |
PhosphoSitePlusi | Q8NCW6 |
Polymorphism and mutation databases
BioMutai | GALNT11 |
DMDMi | 51316030 |
Proteomic databases
EPDi | Q8NCW6 |
jPOSTi | Q8NCW6 |
MassIVEi | Q8NCW6 |
MaxQBi | Q8NCW6 |
PaxDbi | Q8NCW6 |
PeptideAtlasi | Q8NCW6 |
PRIDEi | Q8NCW6 |
ProteomicsDBi | 72960 [Q8NCW6-1] 72961 [Q8NCW6-2] |
Protocols and materials databases
Antibodypediai | 52579, 116 antibodies |
DNASUi | 63917 |
Genome annotation databases
Ensembli | ENST00000415421; ENSP00000410093; ENSG00000178234 [Q8NCW6-2] ENST00000422997; ENSP00000389449; ENSG00000178234 [Q8NCW6-2] ENST00000430044; ENSP00000395122; ENSG00000178234 [Q8NCW6-1] ENST00000434507; ENSP00000416787; ENSG00000178234 [Q8NCW6-1] |
GeneIDi | 63917 |
KEGGi | hsa:63917 |
UCSCi | uc003wku.3, human [Q8NCW6-1] |
Organism-specific databases
CTDi | 63917 |
DisGeNETi | 63917 |
EuPathDBi | HostDB:ENSG00000178234.12 |
GeneCardsi | GALNT11 |
HGNCi | HGNC:19875, GALNT11 |
HPAi | ENSG00000178234, Tissue enhanced (kidney) |
MIMi | 615130, gene |
neXtProti | NX_Q8NCW6 |
OpenTargetsi | ENSG00000178234 |
PharmGKBi | PA134911149 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000158227 |
HOGENOMi | CLU_013477_0_1_1 |
InParanoidi | Q8NCW6 |
OMAi | CDGSLPQ |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q8NCW6 |
TreeFami | TF313267 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.41, 2681 |
PathwayCommonsi | Q8NCW6 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
SABIO-RKi | Q8NCW6 |
Miscellaneous databases
BioGRID-ORCSi | 63917, 0 hits in 844 CRISPR screens |
ChiTaRSi | GALNT11, human |
GenomeRNAii | 63917 |
Pharosi | Q8NCW6, Tbio |
PROi | PR:Q8NCW6 |
RNActi | Q8NCW6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000178234, Expressed in cortex of kidney and 249 other tissues |
ExpressionAtlasi | Q8NCW6, baseline and differential |
Genevisiblei | Q8NCW6, HS |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLT11_HUMAN | |
Accessioni | Q8NCW6Primary (citable) accession number: Q8NCW6 Secondary accession number(s): B3KWF4 Q9UDR8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | August 16, 2004 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations