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Protein

Polypeptide N-acetylgalactosaminyltransferase 6

Gene

GALNT6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. May participate in synthesis of oncofetal fibronectin. Has activity toward Muc1a, Muc2, EA2 and fibronectin peptides.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei217SubstrateBy similarity1
Binding sitei246SubstrateBy similarity1
Metal bindingi269ManganeseBy similarity1
Metal bindingi271ManganeseBy similarity1
Binding sitei379SubstrateBy similarity1
Metal bindingi407ManganeseBy similarity1
Binding sitei410SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • O-glycan processing Source: Reactome
  • protein O-linked glycosylation Source: ProtInc

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandLectin, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.41 2681
ReactomeiR-HSA-913709 O-linked glycosylation of mucins
UniPathwayi
UPA00378

Protein family/group databases

CAZyiCBM13 Carbohydrate-Binding Module Family 13
GT27 Glycosyltransferase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 6 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 6
Short name:
GalNAc-T6
Short name:
pp-GaNTase 6
Protein-UDP acetylgalactosaminyltransferase 6
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6
Gene namesi
Name:GALNT6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000139629.15
HGNCiHGNC:4128 GALNT6
MIMi605148 gene
neXtProtiNX_Q8NCL4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini29 – 622LumenalSequence analysisAdd BLAST594

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi11226
OpenTargetsiENSG00000139629
PharmGKBiPA28541

Polymorphism and mutation databases

BioMutaiGALNT6
DMDMi51316028

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591141 – 622Polypeptide N-acetylgalactosaminyltransferase 6Add BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi165 ↔ 402PROSITE-ProRule annotation
Disulfide bondi393 ↔ 474PROSITE-ProRule annotation
Glycosylationi476N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi509 ↔ 527PROSITE-ProRule annotation
Disulfide bondi553 ↔ 566PROSITE-ProRule annotation
Disulfide bondi597 ↔ 610PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8NCL4
MaxQBiQ8NCL4
PaxDbiQ8NCL4
PeptideAtlasiQ8NCL4
PRIDEiQ8NCL4
ProteomicsDBi72903

PTM databases

iPTMnetiQ8NCL4
PhosphoSitePlusiQ8NCL4

Expressioni

Tissue specificityi

Expressed in placenta and trachea. Weakly expressed in brain and pancreas. Expressed in fibroblast. Weakly or not expressed in lung, liver, muscle, kidney, spleen, thymus, prostate, testis, ovary, intestine, colon, leukocyte, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland and bone marrow.1 Publication

Gene expression databases

BgeeiENSG00000139629 Expressed in 163 organ(s), highest expression level in corpus callosum
CleanExiHS_GALNT6
ExpressionAtlasiQ8NCL4 baseline and differential
GenevisibleiQ8NCL4 HS

Organism-specific databases

HPAiHPA011762
HPA017086

Interactioni

Protein-protein interaction databases

BioGridi116393, 26 interactors
IntActiQ8NCL4, 5 interactors
MINTiQ8NCL4
STRINGi9606.ENSP00000348668

Structurei

3D structure databases

ProteinModelPortaliQ8NCL4
SMRiQ8NCL4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini496 – 622Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST127

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 285Catalytic subdomain AAdd BLAST110
Regioni348 – 410Catalytic subdomain BAdd BLAST63

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3736 Eukaryota
ENOG410XPMK LUCA
GeneTreeiENSGT00760000118828
HOGENOMiHOG000038227
HOVERGENiHBG051699
InParanoidiQ8NCL4
KOiK00710
OMAiSYFEHIG
OrthoDBiEOG091G085O
PhylomeDBiQ8NCL4
TreeFamiTF313267

Family and domain databases

CDDicd00161 RICIN, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS50231 RICIN_B_LECTIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All

Q8NCL4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLLRRRHMP LRLAMVGCAF VLFLFLLHRD VSSREEATEK PWLKSLVSRK
60 70 80 90 100
DHVLDLMLEA MNNLRDSMPK LQIRAPEAQQ TLFSINQSCL PGFYTPAELK
110 120 130 140 150
PFWERPPQDP NAPGADGKAF QKSKWTPLET QEKEEGYKKH CFNAFASDRI
160 170 180 190 200
SLQRSLGPDT RPPECVDQKF RRCPPLATTS VIIVFHNEAW STLLRTVYSV
210 220 230 240 250
LHTTPAILLK EIILVDDAST EEHLKEKLEQ YVKQLQVVRV VRQEERKGLI
260 270 280 290 300
TARLLGASVA QAEVLTFLDA HCECFHGWLE PLLARIAEDK TVVVSPDIVT
310 320 330 340 350
IDLNTFEFAK PVQRGRVHSR GNFDWSLTFG WETLPPHEKQ RRKDETYPIK
360 370 380 390 400
SPTFAGGLFS ISKSYFEHIG TYDNQMEIWG GENVEMSFRV WQCGGQLEII
410 420 430 440 450
PCSVVGHVFR TKSPHTFPKG TSVIARNQVR LAEVWMDSYK KIFYRRNLQA
460 470 480 490 500
AKMAQEKSFG DISERLQLRE QLHCHNFSWY LHNVYPEMFV PDLTPTFYGA
510 520 530 540 550
IKNLGTNQCL DVGENNRGGK PLIMYSCHGL GGNQYFEYTT QRDLRHNIAK
560 570 580 590 600
QLCLHVSKGA LGLGSCHFTG KNSQVPKDEE WELAQDQLIR NSGSGTCLTS
610 620
QDKKPAMAPC NPSDPHQLWL FV
Length:622
Mass (Da):71,159
Last modified:August 16, 2004 - v2
Checksum:i38E63FF2AFB486EF
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R3S5S4R3S5_HUMAN
Polypeptide N-acetylgalactosaminylt...
GALNT6
365Annotation score:
S4R345S4R345_HUMAN
Polypeptide N-acetylgalactosaminylt...
GALNT6
78Annotation score:
S4R3A9S4R3A9_HUMAN
Polypeptide N-acetylgalactosaminylt...
GALNT6
137Annotation score:
S4R310S4R310_HUMAN
Polypeptide N-acetylgalactosaminylt...
GALNT6
68Annotation score:
S4R3M3S4R3M3_HUMAN
Polypeptide N-acetylgalactosaminylt...
GALNT6
123Annotation score:
S4R396S4R396_HUMAN
Polypeptide N-acetylgalactosaminylt...
GALNT6
62Annotation score:
S4R355S4R355_HUMAN
Polypeptide N-acetylgalactosaminylt...
GALNT6
55Annotation score:
S4R3L4S4R3L4_HUMAN
Polypeptide N-acetylgalactosaminylt...
GALNT6
23Annotation score:
S4R370S4R370_HUMAN
Polypeptide N-acetylgalactosaminylt...
GALNT6
39Annotation score:

Sequence cautioni

The sequence BAB15297 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti362S → P in CAA69876 (PubMed:10464263).Curated1
Sequence conflicti454A → T in BAC11118 (PubMed:14702039).Curated1
Sequence conflicti551Q → R in BAC11118 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_019580423V → I. Corresponds to variant dbSNP:rs747300Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08565 mRNA Translation: CAA69876.1
AK025961 mRNA Translation: BAB15297.1 Different initiation.
AK074658 mRNA Translation: BAC11118.1
BC035822 mRNA Translation: AAH35822.2
CCDSiCCDS8813.1
RefSeqiNP_009141.2, NM_007210.3
XP_005268664.1, XM_005268607.1
XP_006719277.1, XM_006719214.2
XP_011536121.1, XM_011537819.2
XP_011536124.1, XM_011537822.2
XP_016874233.1, XM_017018744.1
XP_016874234.1, XM_017018745.1
UniGeneiHs.505575

Genome annotation databases

EnsembliENST00000356317; ENSP00000348668; ENSG00000139629
ENST00000543196; ENSP00000444171; ENSG00000139629
GeneIDi11226
KEGGihsa:11226
UCSCiuc001ryk.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Polypeptide N-acetylgalactosaminyltransferase 6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08565 mRNA Translation: CAA69876.1
AK025961 mRNA Translation: BAB15297.1 Different initiation.
AK074658 mRNA Translation: BAC11118.1
BC035822 mRNA Translation: AAH35822.2
CCDSiCCDS8813.1
RefSeqiNP_009141.2, NM_007210.3
XP_005268664.1, XM_005268607.1
XP_006719277.1, XM_006719214.2
XP_011536121.1, XM_011537819.2
XP_011536124.1, XM_011537822.2
XP_016874233.1, XM_017018744.1
XP_016874234.1, XM_017018745.1
UniGeneiHs.505575

3D structure databases

ProteinModelPortaliQ8NCL4
SMRiQ8NCL4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116393, 26 interactors
IntActiQ8NCL4, 5 interactors
MINTiQ8NCL4
STRINGi9606.ENSP00000348668

Protein family/group databases

CAZyiCBM13 Carbohydrate-Binding Module Family 13
GT27 Glycosyltransferase Family 27

PTM databases

iPTMnetiQ8NCL4
PhosphoSitePlusiQ8NCL4

Polymorphism and mutation databases

BioMutaiGALNT6
DMDMi51316028

Proteomic databases

EPDiQ8NCL4
MaxQBiQ8NCL4
PaxDbiQ8NCL4
PeptideAtlasiQ8NCL4
PRIDEiQ8NCL4
ProteomicsDBi72903

Protocols and materials databases

DNASUi11226
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356317; ENSP00000348668; ENSG00000139629
ENST00000543196; ENSP00000444171; ENSG00000139629
GeneIDi11226
KEGGihsa:11226
UCSCiuc001ryk.3 human

Organism-specific databases

CTDi11226
DisGeNETi11226
EuPathDBiHostDB:ENSG00000139629.15
GeneCardsiGALNT6
HGNCiHGNC:4128 GALNT6
HPAiHPA011762
HPA017086
MIMi605148 gene
neXtProtiNX_Q8NCL4
OpenTargetsiENSG00000139629
PharmGKBiPA28541
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3736 Eukaryota
ENOG410XPMK LUCA
GeneTreeiENSGT00760000118828
HOGENOMiHOG000038227
HOVERGENiHBG051699
InParanoidiQ8NCL4
KOiK00710
OMAiSYFEHIG
OrthoDBiEOG091G085O
PhylomeDBiQ8NCL4
TreeFamiTF313267

Enzyme and pathway databases

UniPathwayi
UPA00378

BRENDAi2.4.1.41 2681
ReactomeiR-HSA-913709 O-linked glycosylation of mucins

Miscellaneous databases

ChiTaRSiGALNT6 human
GeneWikiiGALNT6
GenomeRNAii11226
PROiPR:Q8NCL4
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000139629 Expressed in 163 organ(s), highest expression level in corpus callosum
CleanExiHS_GALNT6
ExpressionAtlasiQ8NCL4 baseline and differential
GenevisibleiQ8NCL4 HS

Family and domain databases

CDDicd00161 RICIN, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS50231 RICIN_B_LECTIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGALT6_HUMAN
AccessioniPrimary (citable) accession number: Q8NCL4
Secondary accession number(s): Q8IYH4, Q9H6G2, Q9UIV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: September 12, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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