UniProtKB - Q8NCF5 (NF2IP_HUMAN)
Protein
NFATC2-interacting protein
Gene
NFATC2IP
Organism
Homo sapiens (Human)
Status
Functioni
In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression (By similarity). Down-regulates formation of poly-SUMO chains by UBE2I/UBC9 (By similarity).By similarity
GO - Biological processi
- cytokine production Source: GO_Central
- positive regulation of transcription by RNA polymerase II Source: GO_Central
Enzyme and pathway databases
PathwayCommonsi | Q8NCF5 |
Names & Taxonomyi
Protein namesi | Recommended name: NFATC2-interacting proteinAlternative name(s): 45 kDa NF-AT-interacting protein Short name: 45 kDa NFAT-interacting protein Nuclear factor of activated T-cells, cytoplasmic 2-interacting protein |
Gene namesi | Name:NFATC2IP Synonyms:NIP45 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25906, NFATC2IP |
MIMi | 614525, gene |
neXtProti | NX_Q8NCF5 |
VEuPathDBi | HostDB:ENSG00000176953.10 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 84901 |
OpenTargetsi | ENSG00000176953 |
PharmGKBi | PA134887712 |
Miscellaneous databases
Pharosi | Q8NCF5, Tbio |
Genetic variation databases
BioMutai | NFATC2IP |
DMDMi | 74751188 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281008 | 1 – 419 | NFATC2-interacting proteinAdd BLAST | 419 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 54 | PhosphoserineBy similarity | 1 | |
Modified residuei | 84 | PhosphoserineCombined sources | 1 | |
Modified residuei | 88 | PhosphoserineBy similarity | 1 | |
Modified residuei | 90 | PhosphoserineBy similarity | 1 | |
Modified residuei | 92 | PhosphoserineBy similarity | 1 | |
Modified residuei | 127 | PhosphoserineCombined sources | 1 | |
Cross-linki | 129 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 131 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 198 | PhosphoserineCombined sources | 1 | |
Modified residuei | 201 | PhosphoserineCombined sources | 1 | |
Modified residuei | 204 | PhosphoserineCombined sources | 1 | |
Modified residuei | 220 | PhosphoserineCombined sources | 1 | |
Modified residuei | 314 | PhosphoserineCombined sources | 1 | |
Modified residuei | 316 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 318 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 369 | PhosphoserineCombined sources | 1 | |
Modified residuei | 390 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Methylation at the N-terminus by PRMT1 modulates interaction with the NFAT complex and results in augmented cytokine production.By similarity
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8NCF5 |
jPOSTi | Q8NCF5 |
MassIVEi | Q8NCF5 |
MaxQBi | Q8NCF5 |
PaxDbi | Q8NCF5 |
PeptideAtlasi | Q8NCF5 |
PRIDEi | Q8NCF5 |
ProteomicsDBi | 72887 [Q8NCF5-1] 72888 [Q8NCF5-2] 72889 [Q8NCF5-3] |
TopDownProteomicsi | Q8NCF5-2 [Q8NCF5-2] |
PTM databases
iPTMneti | Q8NCF5 |
PhosphoSitePlusi | Q8NCF5 |
Expressioni
Gene expression databases
Bgeei | ENSG00000176953, Expressed in tonsil and 248 other tissues |
ExpressionAtlasi | Q8NCF5, baseline and differential |
Genevisiblei | Q8NCF5, HS |
Organism-specific databases
HPAi | ENSG00000176953, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with NFATC2, TRAF1, TRAF2 and PRMT1.
Interacts with UBE2I/UBC9 (By similarity).
By similarityBinary interactionsi
Hide detailsIsoform 2 [Q8NCF5-2]
Protein-protein interaction databases
BioGRIDi | 124341, 37 interactors |
IntActi | Q8NCF5, 27 interactors |
MINTi | Q8NCF5 |
STRINGi | 9606.ENSP00000324792 |
Miscellaneous databases
RNActi | Q8NCF5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q8NCF5 |
SMRi | Q8NCF5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8NCF5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 348 – 419 | Ubiquitin-likePROSITE-ProRule annotationAdd BLAST | 72 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 209 – 231 | Sequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 108 – 112 | Poly-Arg | 5 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1769, Eukaryota |
GeneTreei | ENSGT00390000007119 |
HOGENOMi | CLU_055132_1_0_1 |
InParanoidi | Q8NCF5 |
OMAi | KVRCRAD |
OrthoDBi | 979809at2759 |
PhylomeDBi | Q8NCF5 |
TreeFami | TF328600 |
Family and domain databases
InterProi | View protein in InterPro IPR022617, Rad60/SUMO-like_dom IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF11976, Rad60-SLD, 1 hit |
SMARTi | View protein in SMART SM00213, UBQ, 2 hits |
SUPFAMi | SSF54236, SSF54236, 2 hits |
PROSITEi | View protein in PROSITE PS50053, UBIQUITIN_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NCF5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEPVGKRGR WSGGSGAGRG GRGGWGGRGR RPRAQRSPSR GTLDVVSVDL
60 70 80 90 100
VTDSDEEILE VATARGAADE VEVEPPEPPG PVASRDNSNS DSEGEDRRPA
110 120 130 140 150
GPPREPVRRR RRLVLDPGEA PLVPVYSGKV KSSLRLIPDD LSLLKLYPPG
160 170 180 190 200
DEEEAELADS SGLYHEGSPS PGSPWKTKLR TKDKEEKKKT EFLDLDNSPL
210 220 230 240 250
SPPSPRTKSR THTRALKKLS EVNKRLQDLR SCLSPKPPQG QEQQGQEDEV
260 270 280 290 300
VLVEGPTLPE TPRLFPLKIR CRADLVRLPL RMSEPLQSVV DHMATHLGVS
310 320 330 340 350
PSRILLLFGE TELSPTATPR TLKLGVADII DCVVLTSSPE ATETSQQLQL
360 370 380 390 400
RVQGKEKHQT LEVSLSRDSP LKTLMSHYEE AMGLSGRKLS FFFDGTKLSG
410
RELPADLGME SGDLIEVWG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH3BSZ7 | H3BSZ7_HUMAN | NFATC2-interacting protein | NFATC2IP | 140 | Annotation score: |
Sequence cautioni
The sequence AAM49721 differs from that shown. Reason: Frameshift.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031208 | 33 | R → W. Corresponds to variant dbSNP:rs7201257Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023931 | 1 – 292 | Missing in isoform 3. 2 PublicationsAdd BLAST | 292 | |
Alternative sequenceiVSP_023932 | 1 – 281 | Missing in isoform 2. 1 PublicationAdd BLAST | 281 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK027545 mRNA Translation: BAB55189.1 AK074761 mRNA Translation: BAC11189.1 AK297333 mRNA Translation: BAH12551.1 AK316535 mRNA Translation: BAH14906.1 AF458593 mRNA Translation: AAM49721.1 Frameshift. BC018311 mRNA Translation: AAH18311.2 BC021551 mRNA Translation: AAH21551.2 BC068007 mRNA Translation: AAH68007.1 BC080628 mRNA Translation: AAH80628.1 BC101741 mRNA Translation: AAI01742.1 BC112182 mRNA Translation: AAI12183.1 |
CCDSi | CCDS10645.1 [Q8NCF5-1] |
RefSeqi | NP_116204.3, NM_032815.3 [Q8NCF5-1] |
Genome annotation databases
Ensembli | ENST00000320805; ENSP00000324792; ENSG00000176953 [Q8NCF5-1] ENST00000568148; ENSP00000454958; ENSG00000176953 [Q8NCF5-3] |
GeneIDi | 84901 |
KEGGi | hsa:84901 |
UCSCi | uc002dru.4, human [Q8NCF5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK027545 mRNA Translation: BAB55189.1 AK074761 mRNA Translation: BAC11189.1 AK297333 mRNA Translation: BAH12551.1 AK316535 mRNA Translation: BAH14906.1 AF458593 mRNA Translation: AAM49721.1 Frameshift. BC018311 mRNA Translation: AAH18311.2 BC021551 mRNA Translation: AAH21551.2 BC068007 mRNA Translation: AAH68007.1 BC080628 mRNA Translation: AAH80628.1 BC101741 mRNA Translation: AAI01742.1 BC112182 mRNA Translation: AAI12183.1 |
CCDSi | CCDS10645.1 [Q8NCF5-1] |
RefSeqi | NP_116204.3, NM_032815.3 [Q8NCF5-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JXX | NMR | - | A | 342-419 | [»] | |
2L76 | NMR | - | A | 244-338 | [»] | |
3RD2 | X-ray | 1.60 | A | 345-419 | [»] | |
BMRBi | Q8NCF5 | |||||
SMRi | Q8NCF5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124341, 37 interactors |
IntActi | Q8NCF5, 27 interactors |
MINTi | Q8NCF5 |
STRINGi | 9606.ENSP00000324792 |
PTM databases
iPTMneti | Q8NCF5 |
PhosphoSitePlusi | Q8NCF5 |
Genetic variation databases
BioMutai | NFATC2IP |
DMDMi | 74751188 |
Proteomic databases
EPDi | Q8NCF5 |
jPOSTi | Q8NCF5 |
MassIVEi | Q8NCF5 |
MaxQBi | Q8NCF5 |
PaxDbi | Q8NCF5 |
PeptideAtlasi | Q8NCF5 |
PRIDEi | Q8NCF5 |
ProteomicsDBi | 72887 [Q8NCF5-1] 72888 [Q8NCF5-2] 72889 [Q8NCF5-3] |
TopDownProteomicsi | Q8NCF5-2 [Q8NCF5-2] |
Protocols and materials databases
Antibodypediai | 26645, 82 antibodies |
DNASUi | 84901 |
Genome annotation databases
Ensembli | ENST00000320805; ENSP00000324792; ENSG00000176953 [Q8NCF5-1] ENST00000568148; ENSP00000454958; ENSG00000176953 [Q8NCF5-3] |
GeneIDi | 84901 |
KEGGi | hsa:84901 |
UCSCi | uc002dru.4, human [Q8NCF5-1] |
Organism-specific databases
CTDi | 84901 |
DisGeNETi | 84901 |
GeneCardsi | NFATC2IP |
HGNCi | HGNC:25906, NFATC2IP |
HPAi | ENSG00000176953, Low tissue specificity |
MIMi | 614525, gene |
neXtProti | NX_Q8NCF5 |
OpenTargetsi | ENSG00000176953 |
PharmGKBi | PA134887712 |
VEuPathDBi | HostDB:ENSG00000176953.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1769, Eukaryota |
GeneTreei | ENSGT00390000007119 |
HOGENOMi | CLU_055132_1_0_1 |
InParanoidi | Q8NCF5 |
OMAi | KVRCRAD |
OrthoDBi | 979809at2759 |
PhylomeDBi | Q8NCF5 |
TreeFami | TF328600 |
Enzyme and pathway databases
PathwayCommonsi | Q8NCF5 |
Miscellaneous databases
BioGRID-ORCSi | 84901, 126 hits in 880 CRISPR screens |
ChiTaRSi | NFATC2IP, human |
EvolutionaryTracei | Q8NCF5 |
GenomeRNAii | 84901 |
Pharosi | Q8NCF5, Tbio |
PROi | PR:Q8NCF5 |
RNActi | Q8NCF5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000176953, Expressed in tonsil and 248 other tissues |
ExpressionAtlasi | Q8NCF5, baseline and differential |
Genevisiblei | Q8NCF5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR022617, Rad60/SUMO-like_dom IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF11976, Rad60-SLD, 1 hit |
SMARTi | View protein in SMART SM00213, UBQ, 2 hits |
SUPFAMi | SSF54236, SSF54236, 2 hits |
PROSITEi | View protein in PROSITE PS50053, UBIQUITIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NF2IP_HUMAN | |
Accessioni | Q8NCF5Primary (citable) accession number: Q8NCF5 Secondary accession number(s): B7Z4G5 Q96ST9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references