UniProtKB - Q8NCD3 (HJURP_HUMAN)
Holliday junction recognition protein
HJURP
Functioni
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- histone binding Source: UniProtKB
- identical protein binding Source: IntAct
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- CENP-A containing nucleosome assembly Source: UniProtKB
- chromosome segregation Source: UniProtKB
- regulation of DNA binding Source: UniProtKB
- regulation of protein-containing complex assembly Source: UniProtKB
Keywordsi
Molecular function | Chaperone, DNA-binding |
Biological process | Cell cycle |
Enzyme and pathway databases
PathwayCommonsi | Q8NCD3 |
Reactomei | R-HSA-606279, Deposition of new CENPA-containing nucleosomes at the centromere |
SIGNORi | Q8NCD3 |
Names & Taxonomyi
Protein namesi | Recommended name: Holliday junction recognition proteinAlternative name(s): 14-3-3-associated AKT substrate Fetal liver-expressing gene 1 protein Up-regulated in lung cancer 9 |
Gene namesi | Name:HJURP Synonyms:FAKTS, FLEG1Imported, URLC9 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25444, HJURP |
MIMi | 612667, gene |
neXtProti | NX_Q8NCD3 |
VEuPathDBi | HostDB:ENSG00000123485.11 |
Subcellular locationi
Nucleus
Other locations
Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage.
Mitochondrion
- mitochondrion Source: HPA
Nucleus
- nucleolus Source: UniProtKB
- nucleoplasm Source: HPA
Other locations
- chromosome, centromeric region Source: UniProtKB
- condensed chromosome kinetochore Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 486 | S → A: Loss of phosphorylation by AKT1 and binding to YWHAG. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 55355 |
OpenTargetsi | ENSG00000123485 |
PharmGKBi | PA162390937 |
Miscellaneous databases
Pharosi | Q8NCD3, Tbio |
Genetic variation databases
BioMutai | HJURP |
DMDMi | 239938642 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000287433 | 1 – 748 | Holliday junction recognition proteinAdd BLAST | 748 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 123 | PhosphoserineCombined sources | 1 | |
Modified residuei | 140 | PhosphoserineCombined sources | 1 | |
Modified residuei | 185 | PhosphoserineCombined sources | 1 | |
Modified residuei | 201 | PhosphoserineCombined sources | 1 | |
Modified residuei | 211 | PhosphoserineCombined sources | 1 | |
Cross-linki | 354 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 412 | PhosphoserineBy similarity | 1 | |
Modified residuei | 448 | PhosphoserineCombined sources | 1 | |
Modified residuei | 473 | PhosphoserineCombined sources | 1 | |
Modified residuei | 486 | Phosphoserine; by PKB/AKT1Combined sources1 Publication | 1 | |
Modified residuei | 496 | PhosphoserineCombined sources | 1 | |
Cross-linki | 581 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 586 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 595 | PhosphoserineCombined sources | 1 | |
Modified residuei | 642 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8NCD3 |
jPOSTi | Q8NCD3 |
MassIVEi | Q8NCD3 |
MaxQBi | Q8NCD3 |
PaxDbi | Q8NCD3 |
PeptideAtlasi | Q8NCD3 |
PRIDEi | Q8NCD3 |
ProteomicsDBi | 72875 [Q8NCD3-1] 72876 [Q8NCD3-2] 72877 [Q8NCD3-3] |
PTM databases
GlyConnecti | 2048, 3 N-Linked glycans (1 site) |
GlyGeni | Q8NCD3, 1 site, 3 N-linked glycans (1 site) |
iPTMneti | Q8NCD3 |
MetOSitei | Q8NCD3 |
PhosphoSitePlusi | Q8NCD3 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000123485, Expressed in ventricular zone and 139 other tissues |
ExpressionAtlasi | Q8NCD3, baseline and differential |
Genevisiblei | Q8NCD3, HS |
Organism-specific databases
HPAi | ENSG00000123485, Group enriched (bone marrow, lymphoid tissue, testis) |
Interactioni
Subunit structurei
Interacts with CENPA (via CATD domain); the interaction is direct and specific for CENPA since it does not interact with H3.1- or H3.3-containing nucleosomes (PubMed:16622419, PubMed:19410544, PubMed:19410545). Heterotrimer composed of HJURP, CENPA and histone H4, where HJURP interacts with the dimer formed by CENPA and histone H4 and prevents tetramerization of CENPA and H4 (PubMed:21478274).
Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1 (PubMed:27499292).
Interacts with 14-3-3 family members in a phosphorylation-dependent manner (PubMed:17256767).
Interacts with MSH5 and NBN (PubMed:17823411).
7 PublicationsBinary interactionsi
Hide detailsQ8NCD3
With | #Exp. | IntAct |
---|---|---|
CENPA [P49450] | 15 | EBI-719429,EBI-1751979 |
CENPA - isoform 1 [P49450-1] | 3 | EBI-719429,EBI-15826012 |
itself | 4 | EBI-719429,EBI-719429 |
Isoform 1 [Q8NCD3-1]
With | #Exp. | IntAct |
---|---|---|
CENPA [P49450] | 2 | EBI-15825976,EBI-1751979 |
GO - Molecular functioni
- histone binding Source: UniProtKB
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 120635, 33 interactors |
CORUMi | Q8NCD3 |
DIPi | DIP-53282N |
IntActi | Q8NCD3, 19 interactors |
MINTi | Q8NCD3 |
STRINGi | 9606.ENSP00000414109 |
Miscellaneous databases
RNActi | Q8NCD3, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q8NCD3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502SJZT, Eukaryota |
GeneTreei | ENSGT00390000005575 |
InParanoidi | Q8NCD3 |
OMAi | YGQWASS |
OrthoDBi | 426984at2759 |
PhylomeDBi | Q8NCD3 |
TreeFami | TF336293 |
Family and domain databases
IDEALi | IID00283 |
InterProi | View protein in InterPro IPR021052, HJURP IPR022102, HJURP_C IPR018465, Scm3/HJURP |
Pfami | View protein in Pfam PF12347, HJURP_C, 2 hits PF12346, HJURP_mid, 1 hit PF10384, Scm3, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLGTLRAMEG EDVEDDQLLQ KLRASRRRFQ RRMQRLIEKY NQPFEDTPVV
60 70 80 90 100
QMATLTYETP QGLRIWGGRL IKERNEGEIQ DSSMKPADRT DGSVQAAAWG
110 120 130 140 150
PELPSHRTVL GADSKSGEVD ATSDQEESVA WALAPAVPQS PLKNELRRKY
160 170 180 190 200
LTQVDILLQG AEYFECAGNR AGRDVRVTPL PSLASPAVPA PGYCSRISRK
210 220 230 240 250
SPGDPAKPAS SPREWDPLHP SSTDMALVPR NDSLSLQETS SSSFLSSQPF
260 270 280 290 300
EDDDICNVTI SDLYAGMLHS MSRLLSTKPS SIISTKTFIM QNWNSRRRHR
310 320 330 340 350
YKSRMNKTYC KGARRSQRSS KENFIPCSEP VKGTGALRDC KNVLDVSCRK
360 370 380 390 400
TGLKLEKAFL EVNRPQIHKL DPSWKERKVT PSKYSSLIYF DSSATYNLDE
410 420 430 440 450
ENRFRTLKWL ISPVKIVSRP TIRQGHGENR QREIEIRFDQ LHREYCLSPR
460 470 480 490 500
NQPRRMCLPD SWAMNMYRGG PASPGGLQGL ETRRLSLPSS KAKAKSLSEA
510 520 530 540 550
FENLGKRSLE AGRCLPKSDS SSSLPKTNPT HSATRPQQTS DLHVQGNSSG
560 570 580 590 600
IFRKSVSPSK TLSVPDKEVP GHGRNRYDEI KEEFDKLHQK YCLKSPGQMT
610 620 630 640 650
VPLCIGVSTD KASMEVRYQT EGFLGKLNPD PHFQGFQKLP SSPLGCRKSL
660 670 680 690 700
LGSTAIEAPS STCVARAITR DGTRDHQFPA KRPRLSEPQG SGRQGNSLGA
710 720 730 740
SDGVDNTVRP GDQGSSSQPN SEERGENTSY RMEEKSDFML EKLETKSV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JWC4 | C9JWC4_HUMAN | Holliday junction recognition prote... | HJURP | 570 | Annotation score: | ||
F8WCV1 | F8WCV1_HUMAN | Holliday junction recognition prote... | HJURP | 122 | Annotation score: | ||
H7C3V8 | H7C3V8_HUMAN | Holliday junction recognition prote... | HJURP | 268 | Annotation score: | ||
H7C0I6 | H7C0I6_HUMAN | Holliday junction recognition prote... | HJURP | 68 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 613 | S → C in BAG63122 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 733 | E → G in BAG63122 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_056912 | 4 | T → A. Corresponds to variant dbSNP:rs2302154Ensembl. | 1 | |
Natural variantiVAR_057946 | 76 | E → K4 PublicationsCorresponds to variant dbSNP:rs2286430Ensembl. | 1 | |
Natural variantiVAR_057947 | 199 | R → GCombined sources3 PublicationsCorresponds to variant dbSNP:rs3806589Ensembl. | 1 | |
Natural variantiVAR_057948 | 295 | S → C4 PublicationsCorresponds to variant dbSNP:rs3732215Ensembl. | 1 | |
Natural variantiVAR_056913 | 548 | S → T. Corresponds to variant dbSNP:rs17863822Ensembl. | 1 | |
Natural variantiVAR_056914 | 549 | S → C1 PublicationCorresponds to variant dbSNP:rs3821238Ensembl. | 1 | |
Natural variantiVAR_056915 | 568 | E → D. Corresponds to variant dbSNP:rs3771333Ensembl. | 1 | |
Natural variantiVAR_056916 | 691 | S → F1 PublicationCorresponds to variant dbSNP:rs12582Ensembl. | 1 | |
Natural variantiVAR_057949 | 723 | E → G1 PublicationCorresponds to variant dbSNP:rs10511Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037467 | 81 – 165 | Missing in isoform 3. 1 PublicationAdd BLAST | 85 | |
Alternative sequenceiVSP_037468 | 81 – 134 | Missing in isoform 2. 1 PublicationAdd BLAST | 54 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB101211 mRNA Translation: BAF82039.1 AB162218 mRNA Translation: BAD36741.1 AK301643 mRNA Translation: BAG63122.1 AK303109 mRNA Translation: BAG64216.1 AK074809 mRNA Translation: BAC11221.1 AC005538 Genomic DNA No translation available. BC001940 mRNA Translation: AAH01940.2 AL162048 mRNA Translation: CAB82391.2 Sequence problems. |
CCDSi | CCDS33406.1 [Q8NCD3-1] CCDS63166.1 [Q8NCD3-3] CCDS63167.1 [Q8NCD3-2] |
PIRi | T47163 |
RefSeqi | NP_001269891.1, NM_001282962.1 [Q8NCD3-2] NP_001269892.1, NM_001282963.1 [Q8NCD3-3] NP_060880.3, NM_018410.4 [Q8NCD3-1] |
Genome annotation databases
Ensembli | ENST00000411486; ENSP00000414109; ENSG00000123485 [Q8NCD3-1] ENST00000432087; ENSP00000407208; ENSG00000123485 [Q8NCD3-2] ENST00000441687; ENSP00000401944; ENSG00000123485 [Q8NCD3-3] |
GeneIDi | 55355 |
KEGGi | hsa:55355 |
UCSCi | uc002vvg.5, human [Q8NCD3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB101211 mRNA Translation: BAF82039.1 AB162218 mRNA Translation: BAD36741.1 AK301643 mRNA Translation: BAG63122.1 AK303109 mRNA Translation: BAG64216.1 AK074809 mRNA Translation: BAC11221.1 AC005538 Genomic DNA No translation available. BC001940 mRNA Translation: AAH01940.2 AL162048 mRNA Translation: CAB82391.2 Sequence problems. |
CCDSi | CCDS33406.1 [Q8NCD3-1] CCDS63166.1 [Q8NCD3-3] CCDS63167.1 [Q8NCD3-2] |
PIRi | T47163 |
RefSeqi | NP_001269891.1, NM_001282962.1 [Q8NCD3-2] NP_001269892.1, NM_001282963.1 [Q8NCD3-3] NP_060880.3, NM_018410.4 [Q8NCD3-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3R45 | X-ray | 2.60 | C | 1-80 | [»] | |
SMRi | Q8NCD3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120635, 33 interactors |
CORUMi | Q8NCD3 |
DIPi | DIP-53282N |
IntActi | Q8NCD3, 19 interactors |
MINTi | Q8NCD3 |
STRINGi | 9606.ENSP00000414109 |
PTM databases
GlyConnecti | 2048, 3 N-Linked glycans (1 site) |
GlyGeni | Q8NCD3, 1 site, 3 N-linked glycans (1 site) |
iPTMneti | Q8NCD3 |
MetOSitei | Q8NCD3 |
PhosphoSitePlusi | Q8NCD3 |
Genetic variation databases
BioMutai | HJURP |
DMDMi | 239938642 |
Proteomic databases
EPDi | Q8NCD3 |
jPOSTi | Q8NCD3 |
MassIVEi | Q8NCD3 |
MaxQBi | Q8NCD3 |
PaxDbi | Q8NCD3 |
PeptideAtlasi | Q8NCD3 |
PRIDEi | Q8NCD3 |
ProteomicsDBi | 72875 [Q8NCD3-1] 72876 [Q8NCD3-2] 72877 [Q8NCD3-3] |
Protocols and materials databases
Antibodypediai | 2018, 139 antibodies |
Genome annotation databases
Ensembli | ENST00000411486; ENSP00000414109; ENSG00000123485 [Q8NCD3-1] ENST00000432087; ENSP00000407208; ENSG00000123485 [Q8NCD3-2] ENST00000441687; ENSP00000401944; ENSG00000123485 [Q8NCD3-3] |
GeneIDi | 55355 |
KEGGi | hsa:55355 |
UCSCi | uc002vvg.5, human [Q8NCD3-1] |
Organism-specific databases
CTDi | 55355 |
DisGeNETi | 55355 |
GeneCardsi | HJURP |
HGNCi | HGNC:25444, HJURP |
HPAi | ENSG00000123485, Group enriched (bone marrow, lymphoid tissue, testis) |
MIMi | 612667, gene |
neXtProti | NX_Q8NCD3 |
OpenTargetsi | ENSG00000123485 |
PharmGKBi | PA162390937 |
VEuPathDBi | HostDB:ENSG00000123485.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502SJZT, Eukaryota |
GeneTreei | ENSGT00390000005575 |
InParanoidi | Q8NCD3 |
OMAi | YGQWASS |
OrthoDBi | 426984at2759 |
PhylomeDBi | Q8NCD3 |
TreeFami | TF336293 |
Enzyme and pathway databases
PathwayCommonsi | Q8NCD3 |
Reactomei | R-HSA-606279, Deposition of new CENPA-containing nucleosomes at the centromere |
SIGNORi | Q8NCD3 |
Miscellaneous databases
BioGRID-ORCSi | 55355, 676 hits in 1003 CRISPR screens |
ChiTaRSi | HJURP, human |
GenomeRNAii | 55355 |
Pharosi | Q8NCD3, Tbio |
PROi | PR:Q8NCD3 |
RNActi | Q8NCD3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000123485, Expressed in ventricular zone and 139 other tissues |
ExpressionAtlasi | Q8NCD3, baseline and differential |
Genevisiblei | Q8NCD3, HS |
Family and domain databases
IDEALi | IID00283 |
InterProi | View protein in InterPro IPR021052, HJURP IPR022102, HJURP_C IPR018465, Scm3/HJURP |
Pfami | View protein in Pfam PF12347, HJURP_C, 2 hits PF12346, HJURP_mid, 1 hit PF10384, Scm3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HJURP_HUMAN | |
Accessioni | Q8NCD3Primary (citable) accession number: Q8NCD3 Secondary accession number(s): A8IRH5 Q9NSL8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | June 16, 2009 | |
Last modified: | April 7, 2021 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references