UniProtKB - Q8NBT2 (SPC24_HUMAN)
Kinetochore protein Spc24
SPC24
Functioni
GO - Biological processi
- cell division Source: UniProtKB-KW
- mitotic spindle organization Source: Reactome
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q8NBT2 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
Names & Taxonomyi
Protein namesi | Recommended name: Kinetochore protein Spc24Short name: hSpc24 |
Gene namesi | Name:SPC24 Synonyms:SPBC24 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26913, SPC24 |
MIMi | 609394, gene |
neXtProti | NX_Q8NBT2 |
VEuPathDBi | HostDB:ENSG00000161888.11 |
Subcellular locationi
Nucleus
- Nucleus 4 Publications
Other locations
- kinetochore 4 Publications
Note: Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.1 Publication
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
Other locations
- condensed chromosome kinetochore Source: UniProtKB-SubCell
- Ndc80 complex Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, Kinetochore, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 147841 |
PharmGKBi | PA162404412 |
Miscellaneous databases
Pharosi | Q8NBT2, Tbio |
Genetic variation databases
BioMutai | SPC24 |
DMDMi | 391358156 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249559 | 1 – 197 | Kinetochore protein Spc24Add BLAST | 197 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 11 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8NBT2 |
jPOSTi | Q8NBT2 |
MassIVEi | Q8NBT2 |
MaxQBi | Q8NBT2 |
PaxDbi | Q8NBT2 |
PeptideAtlasi | Q8NBT2 |
PRIDEi | Q8NBT2 |
ProteomicsDBi | 72820 [Q8NBT2-1] |
TopDownProteomicsi | Q8NBT2-1 [Q8NBT2-1] |
PTM databases
iPTMneti | Q8NBT2 |
PhosphoSitePlusi | Q8NBT2 |
Expressioni
Gene expression databases
Bgeei | ENSG00000161888, Expressed in stomach and 129 other tissues |
ExpressionAtlasi | Q8NBT2, baseline and differential |
Genevisiblei | Q8NBT2, HS |
Organism-specific databases
HPAi | ENSG00000161888, Tissue enhanced (lymphoid) |
Interactioni
Subunit structurei
Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.
1 PublicationBinary interactionsi
Hide detailsQ8NBT2
With | #Exp. | IntAct |
---|---|---|
SPC25 [Q9HBM1] | 13 | EBI-999900,EBI-999909 |
SUOX [P51687] | 3 | EBI-999900,EBI-3921347 |
WHRN [Q9P202] | 3 | EBI-999900,EBI-310886 |
Protein-protein interaction databases
BioGRIDi | 127093, 54 interactors |
ComplexPortali | CPX-550, Ndc80 complex |
CORUMi | Q8NBT2 |
DIPi | DIP-35754N |
IntActi | Q8NBT2, 42 interactors |
MINTi | Q8NBT2 |
STRINGi | 9606.ENSP00000465075 |
Miscellaneous databases
RNActi | Q8NBT2, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q8NBT2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8NBT2 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 131 | Interaction with the N-terminus of SPBC25Add BLAST | 131 | |
Regioni | 1 – 69 | Interaction with the NDC80-CDCA1 subcomplexAdd BLAST | 69 | |
Regioni | 132 – 197 | Interaction with the C-terminus of SPBC25Add BLAST | 66 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 33 – 131 | Sequence analysisAdd BLAST | 99 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502S26V, Eukaryota |
HOGENOMi | CLU_119584_0_0_1 |
InParanoidi | Q8NBT2 |
OrthoDBi | 1602618at2759 |
Family and domain databases
IDEALi | IID00388 |
InterProi | View protein in InterPro IPR013252, Ndc80_Spc24 |
PANTHERi | PTHR22142, PTHR22142, 1 hit |
Pfami | View protein in Pfam PF08286, Spc24, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAFRDIEEV SQGLLSLLGA NRAEAQQRRL LGRHEQVVER LLETQDGAEK
60 70 80 90 100
QLREILTMEK EVAQSLLNAK EQVHQGGVEL QQLEAGLQEA GEEDTRLKAS
110 120 130 140 150
LLYLTRELEE LKEIEADLER QEKEVDEDTT VTIPSAVYVA QLYHQVSKIE
160 170 180 190
WDYECEPGMV KGIHHGPSVA QPIHLDSTQL SRKFISDYLW SLVDTEW
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EJH0 | K7EJH0_HUMAN | Kinetochore protein Spc24 | SPC24 | 168 | Annotation score: | ||
K7EMX1 | K7EMX1_HUMAN | Kinetochore protein Spc24 | SPC24 | 171 | Annotation score: | ||
K7ERQ7 | K7ERQ7_HUMAN | Kinetochore protein Spc24 | SPC24 | 126 | Annotation score: | ||
K7ESQ2 | K7ESQ2_HUMAN | Kinetochore protein Spc24 | SPC24 | 209 | Annotation score: | ||
K7EJV2 | K7EJV2_HUMAN | Kinetochore protein Spc24 | SPC24 | 172 | Annotation score: | ||
A0A0A0MSN0 | A0A0A0MSN0_HUMAN | Kinetochore protein Spc24 | SPC24 | 134 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 103 | Y → Q in AAR88651 (PubMed:14738735).Curated | 1 | |
Sequence conflicti | 103 | Y → Q in BAC11523 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 103 | Y → Q in AAI05038 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 103 | Y → Q in AAI05040 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054243 | 72 – 117 | Missing in isoform 2. 1 PublicationAdd BLAST | 46 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY456387 mRNA Translation: AAR88651.1 AK075287 mRNA Translation: BAC11523.1 AK303157 mRNA Translation: BAG64259.1 AC011485 Genomic DNA No translation available. BC105037 mRNA Translation: AAI05038.1 BC105039 mRNA Translation: AAI05040.1 |
CCDSi | CCDS45974.1 [Q8NBT2-1] |
RefSeqi | NP_001303960.1, NM_001317031.1 NP_001303962.1, NM_001317033.1 NP_872319.1, NM_182513.3 XP_005259810.1, XM_005259753.3 [Q8NBT2-2] |
Genome annotation databases
Ensembli | ENST00000592540; ENSP00000465075; ENSG00000161888 |
GeneIDi | 147841 |
KEGGi | hsa:147841 |
UCSCi | uc002mql.3, human [Q8NBT2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY456387 mRNA Translation: AAR88651.1 AK075287 mRNA Translation: BAC11523.1 AK303157 mRNA Translation: BAG64259.1 AC011485 Genomic DNA No translation available. BC105037 mRNA Translation: AAI05038.1 BC105039 mRNA Translation: AAI05040.1 |
CCDSi | CCDS45974.1 [Q8NBT2-1] |
RefSeqi | NP_001303960.1, NM_001317031.1 NP_001303962.1, NM_001317033.1 NP_872319.1, NM_182513.3 XP_005259810.1, XM_005259753.3 [Q8NBT2-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VE7 | X-ray | 2.88 | C/D | 122-197 | [»] | |
3IZ0 | electron microscopy | 8.60 | D/F | 122-197 | [»] | |
SMRi | Q8NBT2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 127093, 54 interactors |
ComplexPortali | CPX-550, Ndc80 complex |
CORUMi | Q8NBT2 |
DIPi | DIP-35754N |
IntActi | Q8NBT2, 42 interactors |
MINTi | Q8NBT2 |
STRINGi | 9606.ENSP00000465075 |
PTM databases
iPTMneti | Q8NBT2 |
PhosphoSitePlusi | Q8NBT2 |
Genetic variation databases
BioMutai | SPC24 |
DMDMi | 391358156 |
Proteomic databases
EPDi | Q8NBT2 |
jPOSTi | Q8NBT2 |
MassIVEi | Q8NBT2 |
MaxQBi | Q8NBT2 |
PaxDbi | Q8NBT2 |
PeptideAtlasi | Q8NBT2 |
PRIDEi | Q8NBT2 |
ProteomicsDBi | 72820 [Q8NBT2-1] |
TopDownProteomicsi | Q8NBT2-1 [Q8NBT2-1] |
Protocols and materials databases
Antibodypediai | 53808, 67 antibodies |
Genome annotation databases
Ensembli | ENST00000592540; ENSP00000465075; ENSG00000161888 |
GeneIDi | 147841 |
KEGGi | hsa:147841 |
UCSCi | uc002mql.3, human [Q8NBT2-1] |
Organism-specific databases
CTDi | 147841 |
DisGeNETi | 147841 |
GeneCardsi | SPC24 |
HGNCi | HGNC:26913, SPC24 |
HPAi | ENSG00000161888, Tissue enhanced (lymphoid) |
MIMi | 609394, gene |
neXtProti | NX_Q8NBT2 |
PharmGKBi | PA162404412 |
VEuPathDBi | HostDB:ENSG00000161888.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S26V, Eukaryota |
HOGENOMi | CLU_119584_0_0_1 |
InParanoidi | Q8NBT2 |
OrthoDBi | 1602618at2759 |
Enzyme and pathway databases
PathwayCommonsi | Q8NBT2 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
Miscellaneous databases
BioGRID-ORCSi | 147841, 798 hits in 1005 CRISPR screens |
ChiTaRSi | SPC24, human |
EvolutionaryTracei | Q8NBT2 |
GeneWikii | SPC24 |
GenomeRNAii | 147841 |
Pharosi | Q8NBT2, Tbio |
PROi | PR:Q8NBT2 |
RNActi | Q8NBT2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000161888, Expressed in stomach and 129 other tissues |
ExpressionAtlasi | Q8NBT2, baseline and differential |
Genevisiblei | Q8NBT2, HS |
Family and domain databases
IDEALi | IID00388 |
InterProi | View protein in InterPro IPR013252, Ndc80_Spc24 |
PANTHERi | PTHR22142, PTHR22142, 1 hit |
Pfami | View protein in Pfam PF08286, Spc24, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPC24_HUMAN | |
Accessioni | Q8NBT2Primary (citable) accession number: Q8NBT2 Secondary accession number(s): B4DZZ7, C9JGC4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2006 |
Last sequence update: | June 13, 2012 | |
Last modified: | April 7, 2021 | |
This is version 146 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families