SPC24

Functionⁱ

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).2 Publications

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
cell division Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cell cycle, Cell division, Mitosis

Biological process

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

Reactomeⁱ	R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813 Separation of Sister Chromatids R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-5663220 RHO GTPases Activate Formins R-HSA-68877 Mitotic Prometaphase

Reactomeⁱ

R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Kinetochore protein Spc24 Short name: hSpc24
Gene namesⁱ	Name:SPC24 Synonyms:SPBC24
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 19

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000161888.11
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:26913 SPC24
MIMⁱ	609394 gene
neXtProtⁱ	NX_Q8NBT2

Keywords - Cellular componentⁱ

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	147841
PharmGKBⁱ	PA162404412

Polymorphism and mutation databases

BioMutaⁱ	SPC24
DMDMⁱ	391358156

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000249559	1 – 197	Kinetochore protein Spc24Add BLAST		197

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	11	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q8NBT2
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q8NBT2
PaxDbⁱ	Q8NBT2
PeptideAtlas More...PeptideAtlasⁱ	Q8NBT2
PRIDEⁱ	Q8NBT2
ProteomicsDBⁱ	72820
TopDownProteomicsⁱ	Q8NBT2-1 [Q8NBT2-1]

PTM databases

iPTMnetⁱ	Q8NBT2
PhosphoSitePlusⁱ	Q8NBT2

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000161888 Expressed in 108 organ(s), highest expression level in lung
CleanExⁱ	HS_SPC24
ExpressionAtlasⁱ	Q8NBT2 baseline and differential
Genevisibleⁱ	Q8NBT2 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA051234

HPAⁱ

HPA051234

Interactionⁱ

Subunit structureⁱ

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
SPC25	Q9HBM1	10	EBI-999900,EBI-999909

With

Entry

#Exp.

IntAct

Notes

SPC25

Q9HBM1

10

EBI-999900,EBI-999909

Protein-protein interaction databases

BioGridⁱ	127093, 48 interactors
ComplexPortalⁱ	CPX-550 Ndc80 complex
CORUMⁱ	Q8NBT2
Database of interacting proteins More...DIPⁱ	DIP-35754N
IntActⁱ	Q8NBT2, 32 interactors
Molecular INTeraction database More...MINTⁱ	Q8NBT2
STRINGⁱ	9606.ENSP00000465075

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	127 – 131	Combined sources	5
Turnⁱ	134 – 136	Combined sources	3
Helixⁱ	137 – 147	Combined sources	11
Turnⁱ	156 – 158	Combined sources	3
Beta strandⁱ	160 – 163	Combined sources	4
Beta strandⁱ	172 – 175	Combined sources	4
Helixⁱ	182 – 190	Combined sources	9

Show more details

3D structure databases

ProteinModelPortalⁱ	Q8NBT2
SMRⁱ	Q8NBT2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

Q8NBT2

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 131	Interaction with the N-terminus of SPBC25Add BLAST	131
Regionⁱ	1 – 69	Interaction with the NDC80-CDCA1 subcomplexAdd BLAST	69
Regionⁱ	132 – 197	Interaction with the C-terminus of SPBC25Add BLAST	66

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	33 – 131	Sequence analysisAdd BLAST		99

Sequence similaritiesⁱ

Belongs to the SPC24 family.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	ENOG410IYQI Eukaryota ENOG4111TPD LUCA
HOGENOMⁱ	HOG000004855
HOVERGENⁱ	HBG083027
InParanoidⁱ	Q8NBT2
KEGG Orthology (KO) More...KOⁱ	K11549

Family and domain databases

InterProⁱ	View protein in InterPro IPR013252 Ndc80_Spc24
PANTHERⁱ	PTHR22142 PTHR22142, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08286 Spc24, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q8NBT2-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAFRDIEEV SQGLLSLLGA NRAEAQQRRL LGRHEQVVER LLETQDGAEK 
        60         70         80         90        100
QLREILTMEK EVAQSLLNAK EQVHQGGVEL QQLEAGLQEA GEEDTRLKAS 
       110        120        130        140        150
LLYLTRELEE LKEIEADLER QEKEVDEDTT VTIPSAVYVA QLYHQVSKIE 
       160        170        180        190 
WDYECEPGMV KGIHHGPSVA QPIHLDSTQL SRKFISDYLW SLVDTEW

Length:197

Mass (Da):22,478

Last modified:June 13, 2012 - v2

Checksum:ⁱC5D99CE1F1A8F355

GO

Isoform 2 (identifier: Q8NBT2-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
72-117: Missing.

« Hide

        10         20         30         40         50
MAAFRDIEEV SQGLLSLLGA NRAEAQQRRL LGRHEQVVER LLETQDGAEK 
        60         70         80         90        100
QLREILTMEK EVAQSLLNAK ELERQEKEVD EDTTVTIPSA VYVAQLYHQV 
       110        120        130        140        150
SKIEWDYECE PGMVKGIHHG PSVAQPIHLD STQLSRKFIS DYLWSLVDTE 

W

Note: No experimental confirmation available.

Show »

Length:151

Mass (Da):17,316

Checksum:ⁱCA84C4FEA046ECD2

GO

Computationally mapped potential isoform sequencesⁱ

There are 6 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

K7EJH0	K7EJH0_HUMAN	Kinetochore protein Spc24 Kinetochore protein Spc24	SPC24	168	Annotation score:
K7ESQ2	K7ESQ2_HUMAN	Kinetochore protein Spc24 Kinetochore protein Spc24	SPC24	209	Annotation score:
K7EJV2	K7EJV2_HUMAN	Kinetochore protein Spc24 Kinetochore protein Spc24	SPC24	172	Annotation score:
K7EMX1	K7EMX1_HUMAN	Kinetochore protein Spc24 Kinetochore protein Spc24	SPC24	171	Annotation score:
A0A0A0MSN0	A0A0A0MSN0_HUMAN	Kinetochore protein Spc24 Kinetochore protein Spc24	SPC24	134	Annotation score:
K7ERQ7	K7ERQ7_HUMAN	Kinetochore protein Spc24 Kinetochore protein Spc24	SPC24	126	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	103	Y → Q in AAR88651 (PubMed:14738735).Curated	1
Sequence conflictⁱ	103	Y → Q in BAC11523 (PubMed:14702039).Curated	1
Sequence conflictⁱ	103	Y → Q in AAI05038 (PubMed:15489334).Curated	1
Sequence conflictⁱ	103	Y → Q in AAI05040 (PubMed:15489334).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_054243	72 – 117	Missing in isoform 2. 1 PublicationAdd BLAST		46

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY456387 mRNA Translation: AAR88651.1 AK075287 mRNA Translation: BAC11523.1 AK303157 mRNA Translation: BAG64259.1 AC011485 Genomic DNA No translation available. BC105037 mRNA Translation: AAI05038.1 BC105039 mRNA Translation: AAI05040.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS45974.1 [Q8NBT2-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001303960.1, NM_001317031.1 NP_001303962.1, NM_001317033.1 NP_872319.1, NM_182513.3 XP_005259810.1, XM_005259753.3 [Q8NBT2-2]
UniGeneⁱ	Hs.381225 Hs.736069

Genome annotation databases

Ensemblⁱ	ENST00000592540; ENSP00000465075; ENSG00000161888
GeneIDⁱ	147841
KEGGⁱ	hsa:147841
UCSC genome browser More...UCSCⁱ	uc002mql.3 human [Q8NBT2-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8NBT2	Uncharacterized protein		Cebus capucinus imitator		223	UniRef90_Q8NBT2
Uncharacterized protein		MACNE		197
Uncharacterized protein		PONAB		197
Uncharacterized protein		GORGO		197
Kinetochore protein Spc24		MACMU		197
+15
Q8NBT2-2	Uncharacterized protein		MACFA		151	UniRef90_Q8NBT2-2
UPI0001D54BFE		MACMU		154

Protein	Similar proteins		Species	Score	Length	Source
Q8NBT2	Kinetochore protein Spc24		BOVIN		197	UniRef50_Q8NBT2
Kinetochore protein Spc24		MOUSE		201
Uncharacterized protein		Cebus capucinus imitator		223
Uncharacterized protein		PONAB		197
SPC24 isoform 1		PANTR		197
+67
Q8NBT2-2	Uncharacterized protein		MACFA		151	UniRef50_Q8NBT2-2
Kinetochore protein Spc24		MOUSE		160
Kinetochore protein Spc24 (Fragment)		HUMAN		172
SPC24 isoform 5 (Fragment)		PANTR		171
SPC24 isoform 5 (Fragment)		PONAB		171
+11

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY456387 mRNA Translation: AAR88651.1 AK075287 mRNA Translation: BAC11523.1 AK303157 mRNA Translation: BAG64259.1 AC011485 Genomic DNA No translation available. BC105037 mRNA Translation: AAI05038.1 BC105039 mRNA Translation: AAI05040.1
CCDSⁱ	CCDS45974.1 [Q8NBT2-1]
RefSeqⁱ	NP_001303960.1, NM_001317031.1 NP_001303962.1, NM_001317033.1 NP_872319.1, NM_182513.3 XP_005259810.1, XM_005259753.3 [Q8NBT2-2]
UniGeneⁱ	Hs.381225 Hs.736069

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2VE7	X-ray	2.88	C/D	122-197	[»]
	3IZ0	electron microscopy	8.60	D/F	122-197	[»]
ProteinModelPortalⁱ	Q8NBT2
SMRⁱ	Q8NBT2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	127093, 48 interactors
ComplexPortalⁱ	CPX-550 Ndc80 complex
CORUMⁱ	Q8NBT2
DIPⁱ	DIP-35754N
IntActⁱ	Q8NBT2, 32 interactors
MINTⁱ	Q8NBT2
STRINGⁱ	9606.ENSP00000465075

PTM databases

iPTMnetⁱ	Q8NBT2
PhosphoSitePlusⁱ	Q8NBT2

Polymorphism and mutation databases

BioMutaⁱ	SPC24
DMDMⁱ	391358156

Proteomic databases

EPDⁱ	Q8NBT2
MaxQBⁱ	Q8NBT2
PaxDbⁱ	Q8NBT2
PeptideAtlasⁱ	Q8NBT2
PRIDEⁱ	Q8NBT2
ProteomicsDBⁱ	72820
TopDownProteomicsⁱ	Q8NBT2-1 [Q8NBT2-1]

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000592540; ENSP00000465075; ENSG00000161888
GeneIDⁱ	147841
KEGGⁱ	hsa:147841
UCSCⁱ	uc002mql.3 human [Q8NBT2-1]

Organism-specific databases

CTDⁱ	147841
DisGeNETⁱ	147841
EuPathDBⁱ	HostDB:ENSG00000161888.11
GeneCardsⁱ	SPC24
HGNCⁱ	HGNC:26913 SPC24
HPAⁱ	HPA051234
MIMⁱ	609394 gene
neXtProtⁱ	NX_Q8NBT2
PharmGKBⁱ	PA162404412
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IYQI Eukaryota ENOG4111TPD LUCA
HOGENOMⁱ	HOG000004855
HOVERGENⁱ	HBG083027
InParanoidⁱ	Q8NBT2
KOⁱ	K11549

Enzyme and pathway databases

Reactomeⁱ	R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813 Separation of Sister Chromatids R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-5663220 RHO GTPases Activate Formins R-HSA-68877 Mitotic Prometaphase

Reactomeⁱ

R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Miscellaneous databases

EvolutionaryTraceⁱ	Q8NBT2
GeneWikiⁱ	SPC24
GenomeRNAiⁱ	147841
Protein Ontology More...PROⁱ	PR:Q8NBT2
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000161888 Expressed in 108 organ(s), highest expression level in lung
CleanExⁱ	HS_SPC24
ExpressionAtlasⁱ	Q8NBT2 baseline and differential
Genevisibleⁱ	Q8NBT2 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR013252 Ndc80_Spc24
PANTHERⁱ	PTHR22142 PTHR22142, 1 hit
Pfamⁱ	View protein in Pfam PF08286 Spc24, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SPC24_HUMAN
Accessionⁱ	Q8NBT2Primary (citable) accession number: Q8NBT2 Secondary accession number(s): B4DZZ7, C9JGC4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
	Last sequence update:	June 13, 2012
	Last modified:	September 12, 2018
	This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM

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UniProtKB - Q8NBT2 (SPC24_HUMAN)

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Kinetochore protein Spc24

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ