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Protein

Kinetochore protein Spc24

Gene

SPC24

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).2 Publications

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Spc24
Short name:
hSpc24
Gene namesi
Name:SPC24
Synonyms:SPBC24
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000161888.11
HGNCiHGNC:26913 SPC24
MIMi609394 gene
neXtProtiNX_Q8NBT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi147841
PharmGKBiPA162404412

Polymorphism and mutation databases

BioMutaiSPC24
DMDMi391358156

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495591 – 197Kinetochore protein Spc24Add BLAST197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8NBT2
MaxQBiQ8NBT2
PaxDbiQ8NBT2
PeptideAtlasiQ8NBT2
PRIDEiQ8NBT2
ProteomicsDBi72820
TopDownProteomicsiQ8NBT2-1 [Q8NBT2-1]

PTM databases

iPTMnetiQ8NBT2
PhosphoSitePlusiQ8NBT2

Expressioni

Gene expression databases

BgeeiENSG00000161888 Expressed in 108 organ(s), highest expression level in lung
CleanExiHS_SPC24
ExpressionAtlasiQ8NBT2 baseline and differential
GenevisibleiQ8NBT2 HS

Organism-specific databases

HPAiHPA051234

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SPC25Q9HBM110EBI-999900,EBI-999909

Protein-protein interaction databases

BioGridi127093, 48 interactors
ComplexPortaliCPX-550 Ndc80 complex
CORUMiQ8NBT2
DIPiDIP-35754N
IntActiQ8NBT2, 32 interactors
MINTiQ8NBT2
STRINGi9606.ENSP00000465075

Structurei

Secondary structure

1197
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8NBT2
SMRiQ8NBT2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NBT2

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 131Interaction with the N-terminus of SPBC25Add BLAST131
Regioni1 – 69Interaction with the NDC80-CDCA1 subcomplexAdd BLAST69
Regioni132 – 197Interaction with the C-terminus of SPBC25Add BLAST66

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili33 – 131Sequence analysisAdd BLAST99

Sequence similaritiesi

Belongs to the SPC24 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IYQI Eukaryota
ENOG4111TPD LUCA
HOGENOMiHOG000004855
HOVERGENiHBG083027
InParanoidiQ8NBT2
KOiK11549

Family and domain databases

InterProiView protein in InterPro
IPR013252 Ndc80_Spc24
PANTHERiPTHR22142 PTHR22142, 1 hit
PfamiView protein in Pfam
PF08286 Spc24, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8NBT2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAFRDIEEV SQGLLSLLGA NRAEAQQRRL LGRHEQVVER LLETQDGAEK
60 70 80 90 100
QLREILTMEK EVAQSLLNAK EQVHQGGVEL QQLEAGLQEA GEEDTRLKAS
110 120 130 140 150
LLYLTRELEE LKEIEADLER QEKEVDEDTT VTIPSAVYVA QLYHQVSKIE
160 170 180 190
WDYECEPGMV KGIHHGPSVA QPIHLDSTQL SRKFISDYLW SLVDTEW
Length:197
Mass (Da):22,478
Last modified:June 13, 2012 - v2
Checksum:iC5D99CE1F1A8F355
GO
Isoform 2 (identifier: Q8NBT2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     72-117: Missing.

Note: No experimental confirmation available.
Show »
Length:151
Mass (Da):17,316
Checksum:iCA84C4FEA046ECD2
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EJH0K7EJH0_HUMAN
Kinetochore protein Spc24
SPC24
168Annotation score:
K7ESQ2K7ESQ2_HUMAN
Kinetochore protein Spc24
SPC24
209Annotation score:
K7EJV2K7EJV2_HUMAN
Kinetochore protein Spc24
SPC24
172Annotation score:
K7EMX1K7EMX1_HUMAN
Kinetochore protein Spc24
SPC24
171Annotation score:
A0A0A0MSN0A0A0A0MSN0_HUMAN
Kinetochore protein Spc24
SPC24
134Annotation score:
K7ERQ7K7ERQ7_HUMAN
Kinetochore protein Spc24
SPC24
126Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103Y → Q in AAR88651 (PubMed:14738735).Curated1
Sequence conflicti103Y → Q in BAC11523 (PubMed:14702039).Curated1
Sequence conflicti103Y → Q in AAI05038 (PubMed:15489334).Curated1
Sequence conflicti103Y → Q in AAI05040 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05424372 – 117Missing in isoform 2. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY456387 mRNA Translation: AAR88651.1
AK075287 mRNA Translation: BAC11523.1
AK303157 mRNA Translation: BAG64259.1
AC011485 Genomic DNA No translation available.
BC105037 mRNA Translation: AAI05038.1
BC105039 mRNA Translation: AAI05040.1
CCDSiCCDS45974.1 [Q8NBT2-1]
RefSeqiNP_001303960.1, NM_001317031.1
NP_001303962.1, NM_001317033.1
NP_872319.1, NM_182513.3
XP_005259810.1, XM_005259753.3 [Q8NBT2-2]
UniGeneiHs.381225
Hs.736069

Genome annotation databases

EnsembliENST00000592540; ENSP00000465075; ENSG00000161888
GeneIDi147841
KEGGihsa:147841
UCSCiuc002mql.3 human [Q8NBT2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY456387 mRNA Translation: AAR88651.1
AK075287 mRNA Translation: BAC11523.1
AK303157 mRNA Translation: BAG64259.1
AC011485 Genomic DNA No translation available.
BC105037 mRNA Translation: AAI05038.1
BC105039 mRNA Translation: AAI05040.1
CCDSiCCDS45974.1 [Q8NBT2-1]
RefSeqiNP_001303960.1, NM_001317031.1
NP_001303962.1, NM_001317033.1
NP_872319.1, NM_182513.3
XP_005259810.1, XM_005259753.3 [Q8NBT2-2]
UniGeneiHs.381225
Hs.736069

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VE7X-ray2.88C/D122-197[»]
3IZ0electron microscopy8.60D/F122-197[»]
ProteinModelPortaliQ8NBT2
SMRiQ8NBT2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127093, 48 interactors
ComplexPortaliCPX-550 Ndc80 complex
CORUMiQ8NBT2
DIPiDIP-35754N
IntActiQ8NBT2, 32 interactors
MINTiQ8NBT2
STRINGi9606.ENSP00000465075

PTM databases

iPTMnetiQ8NBT2
PhosphoSitePlusiQ8NBT2

Polymorphism and mutation databases

BioMutaiSPC24
DMDMi391358156

Proteomic databases

EPDiQ8NBT2
MaxQBiQ8NBT2
PaxDbiQ8NBT2
PeptideAtlasiQ8NBT2
PRIDEiQ8NBT2
ProteomicsDBi72820
TopDownProteomicsiQ8NBT2-1 [Q8NBT2-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000592540; ENSP00000465075; ENSG00000161888
GeneIDi147841
KEGGihsa:147841
UCSCiuc002mql.3 human [Q8NBT2-1]

Organism-specific databases

CTDi147841
DisGeNETi147841
EuPathDBiHostDB:ENSG00000161888.11
GeneCardsiSPC24
HGNCiHGNC:26913 SPC24
HPAiHPA051234
MIMi609394 gene
neXtProtiNX_Q8NBT2
PharmGKBiPA162404412
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IYQI Eukaryota
ENOG4111TPD LUCA
HOGENOMiHOG000004855
HOVERGENiHBG083027
InParanoidiQ8NBT2
KOiK11549

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

Miscellaneous databases

EvolutionaryTraceiQ8NBT2
GeneWikiiSPC24
GenomeRNAii147841
PROiPR:Q8NBT2
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161888 Expressed in 108 organ(s), highest expression level in lung
CleanExiHS_SPC24
ExpressionAtlasiQ8NBT2 baseline and differential
GenevisibleiQ8NBT2 HS

Family and domain databases

InterProiView protein in InterPro
IPR013252 Ndc80_Spc24
PANTHERiPTHR22142 PTHR22142, 1 hit
PfamiView protein in Pfam
PF08286 Spc24, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPC24_HUMAN
AccessioniPrimary (citable) accession number: Q8NBT2
Secondary accession number(s): B4DZZ7, C9JGC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 13, 2012
Last modified: September 12, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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