UniProtKB - Q8NBN7 (RDH13_HUMAN)
Protein
Retinol dehydrogenase 13
Gene
RDH13
Organism
Homo sapiens (Human)
Status
Functioni
Retinol dehydrogenase with a clear preference for NADP. Oxidizes all-trans-retinol, but seems to reduce all-trans-retinal with much higher efficiency (PubMed:18039331). Has no activity toward steroids (PubMed:18039331).1 Publication
Caution
Was originally thought to lack retinol dehydrogenase (RDH) activity. However, a more recent publication demonstrates a retinol dehydrogenase activity for RDH13.2 Publications
Catalytic activityi
- EC:1.1.1.3001 Publication
Kineticsi
- KM=3.2 µM for all-trans-retinal1 Publication
- KM=3 µM for all-trans-retinol1 Publication
- KM=1.5 µM for NADPH1 Publication
- KM=6000 µM for NAD1 Publication
- Vmax=230 nmol/min/mg enzyme with all-trans-retinal as substrate1 Publication
- Vmax=5 nmol/min/mg enzyme with all-trans-retinol as substrate1 Publication
- Vmax=230 nmol/min/mg enzyme with NADPH as substrate1 Publication
: retinol metabolism Pathwayi
This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 174 | SubstrateBy similarity | 1 | |
Active sitei | 200 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 45 – 51 | NADPBy similarity | 7 |
GO - Molecular functioni
- NADP-retinol dehydrogenase activity Source: UniProtKB
GO - Biological processi
- eye photoreceptor cell development Source: Ensembl
- response to high light intensity Source: Ensembl
- retina layer formation Source: Ensembl
- retinal metabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.300, 2681 |
PathwayCommonsi | Q8NBN7 |
Reactomei | R-HSA-5365859, RA biosynthesis pathway |
UniPathwayi | UPA00912 |
Chemistry databases
SwissLipidsi | SLP:000001877 |
Names & Taxonomyi
Protein namesi | Recommended name: Retinol dehydrogenase 13Curated (EC:1.1.1.3001 Publication)Alternative name(s): Short chain dehydrogenase/reductase family 7C member 3 |
Gene namesi | Name:RDH13 Synonyms:SDR7C3 ORF Names:PSEC0082, UNQ736/PRO1430 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19978, RDH13 |
neXtProti | NX_Q8NBN7 |
VEuPathDBi | HostDB:ENSG00000160439.15 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Localized on the outer side of the inner mitochondrial membrane.1 Publication
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Organism-specific databases
DisGeNETi | 112724 |
OpenTargetsi | ENSG00000160439 |
PharmGKBi | PA134897935 |
Miscellaneous databases
Pharosi | Q8NBN7, Tdark |
Chemistry databases
DrugBanki | DB00162, Vitamin A |
DrugCentrali | Q8NBN7 |
Genetic variation databases
BioMutai | RDH13 |
DMDMi | 62298570 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000054768 | 2 – 331 | Retinol dehydrogenase 13Add BLAST | 330 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 323 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8NBN7 |
jPOSTi | Q8NBN7 |
MassIVEi | Q8NBN7 |
MaxQBi | Q8NBN7 |
PaxDbi | Q8NBN7 |
PeptideAtlasi | Q8NBN7 |
PRIDEi | Q8NBN7 |
ProteomicsDBi | 72802 [Q8NBN7-1] 72803 [Q8NBN7-2] |
PTM databases
iPTMneti | Q8NBN7 |
PhosphoSitePlusi | Q8NBN7 |
Expressioni
Tissue specificityi
Widely expressed (PubMed:18039331). In the retina, detected in the inner segment of the photoreceptor cells. Weak signals are observed in a small population of inner nuclear neurons and the inner plexiform layer (PubMed:12226107).2 Publications
Gene expression databases
Bgeei | ENSG00000160439, Expressed in placenta and 217 other tissues |
ExpressionAtlasi | Q8NBN7, baseline and differential |
Genevisiblei | Q8NBN7, HS |
Organism-specific databases
HPAi | ENSG00000160439, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 125200, 53 interactors |
IntActi | Q8NBN7, 27 interactors |
MINTi | Q8NBN7 |
STRINGi | 9606.ENSP00000391121 |
Miscellaneous databases
RNActi | Q8NBN7, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
eggNOGi | KOG1208, Eukaryota |
GeneTreei | ENSGT00940000159641 |
HOGENOMi | CLU_010194_44_5_1 |
InParanoidi | Q8NBN7 |
OMAi | WQKRKYN |
OrthoDBi | 921996at2759 |
PhylomeDBi | Q8NBN7 |
TreeFami | TF105429 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8NBN7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSRYLLPLSA LGTVAGAAVL LKDYVTGGAC PSKATIPGKT VIVTGANTGI
60 70 80 90 100
GKQTALELAR RGGNIILACR DMEKCEAAAK DIRGETLNHH VNARHLDLAS
110 120 130 140 150
LKSIREFAAK IIEEEERVDI LINNAGVMRC PHWTTEDGFE MQFGVNHLGH
160 170 180 190 200
FLLTNLLLDK LKASAPSRII NLSSLAHVAG HIDFDDLNWQ TRKYNTKAAY
210 220 230 240 250
CQSKLAIVLF TKELSRRLQG SGVTVNALHP GVARTELGRH TGIHGSTFSS
260 270 280 290 300
TTLGPIFWLL VKSPELAAQP STYLAVAEEL ADVSGKYFDG LKQKAPAPEA
310 320 330
EDEEVARRLW AESARLVGLE APSVREQPLP R
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG8JLA1 | G8JLA1_HUMAN | Retinol dehydrogenase 13 | RDH13 | 220 | Annotation score: | ||
K7EMY5 | K7EMY5_HUMAN | Retinol dehydrogenase 13 | RDH13 | 42 | Annotation score: | ||
K7EQB5 | K7EQB5_HUMAN | Retinol dehydrogenase 13 | RDH13 | 29 | Annotation score: | ||
K7ERV1 | K7ERV1_HUMAN | Retinol dehydrogenase 13 | RDH13 | 35 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 106 | E → V in BAC11591 (PubMed:16303743).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040383 | 1 – 71 | Missing in isoform 2. 1 PublicationAdd BLAST | 71 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY358473 mRNA Translation: AAQ88837.1 AK075392 mRNA Translation: BAC11591.1 AC011476 Genomic DNA No translation available. BC009881 mRNA Translation: AAH09881.1 |
CCDSi | CCDS42627.1 [Q8NBN7-2] CCDS54320.1 [Q8NBN7-1] |
RefSeqi | NP_001139443.1, NM_001145971.1 [Q8NBN7-1] NP_612421.1, NM_138412.3 [Q8NBN7-2] XP_005258530.1, XM_005258473.1 [Q8NBN7-1] XP_011524709.1, XM_011526407.1 |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY358473 mRNA Translation: AAQ88837.1 AK075392 mRNA Translation: BAC11591.1 AC011476 Genomic DNA No translation available. BC009881 mRNA Translation: AAH09881.1 |
CCDSi | CCDS42627.1 [Q8NBN7-2] CCDS54320.1 [Q8NBN7-1] |
RefSeqi | NP_001139443.1, NM_001145971.1 [Q8NBN7-1] NP_612421.1, NM_138412.3 [Q8NBN7-2] XP_005258530.1, XM_005258473.1 [Q8NBN7-1] XP_011524709.1, XM_011526407.1 |
3D structure databases
SMRi | Q8NBN7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 125200, 53 interactors |
IntActi | Q8NBN7, 27 interactors |
MINTi | Q8NBN7 |
STRINGi | 9606.ENSP00000391121 |
Chemistry databases
DrugBanki | DB00162, Vitamin A |
DrugCentrali | Q8NBN7 |
SwissLipidsi | SLP:000001877 |
PTM databases
iPTMneti | Q8NBN7 |
PhosphoSitePlusi | Q8NBN7 |
Genetic variation databases
BioMutai | RDH13 |
DMDMi | 62298570 |
Proteomic databases
EPDi | Q8NBN7 |
jPOSTi | Q8NBN7 |
MassIVEi | Q8NBN7 |
MaxQBi | Q8NBN7 |
PaxDbi | Q8NBN7 |
PeptideAtlasi | Q8NBN7 |
PRIDEi | Q8NBN7 |
ProteomicsDBi | 72802 [Q8NBN7-1] 72803 [Q8NBN7-2] |
Protocols and materials databases
Antibodypediai | 32994, 134 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 112724 |
DisGeNETi | 112724 |
GeneCardsi | RDH13 |
HGNCi | HGNC:19978, RDH13 |
HPAi | ENSG00000160439, Low tissue specificity |
neXtProti | NX_Q8NBN7 |
OpenTargetsi | ENSG00000160439 |
PharmGKBi | PA134897935 |
VEuPathDBi | HostDB:ENSG00000160439.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1208, Eukaryota |
GeneTreei | ENSGT00940000159641 |
HOGENOMi | CLU_010194_44_5_1 |
InParanoidi | Q8NBN7 |
OMAi | WQKRKYN |
OrthoDBi | 921996at2759 |
PhylomeDBi | Q8NBN7 |
TreeFami | TF105429 |
Enzyme and pathway databases
UniPathwayi | UPA00912 |
BRENDAi | 1.1.1.300, 2681 |
PathwayCommonsi | Q8NBN7 |
Reactomei | R-HSA-5365859, RA biosynthesis pathway |
Miscellaneous databases
BioGRID-ORCSi | 112724, 5 hits in 990 CRISPR screens |
ChiTaRSi | RDH13, human |
GenomeRNAii | 112724 |
Pharosi | Q8NBN7, Tdark |
PROi | PR:Q8NBN7 |
RNActi | Q8NBN7, protein |
Gene expression databases
Bgeei | ENSG00000160439, Expressed in placenta and 217 other tissues |
ExpressionAtlasi | Q8NBN7, baseline and differential |
Genevisiblei | Q8NBN7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RDH13_HUMAN | |
Accessioni | Q8NBN7Primary (citable) accession number: Q8NBN7 Secondary accession number(s): Q6UX79, Q96G88 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 29, 2003 |
Last sequence update: | March 29, 2005 | |
Last modified: | April 7, 2021 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families