UniProtKB - Q8N9I9 (DTX3_HUMAN)
Protein
Probable E3 ubiquitin-protein ligase DTX3
Gene
DTX3
Organism
Homo sapiens (Human)
Status
Functioni
Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context (By similarity). Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.By similarity
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 164 – 205 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: GO_Central
GO - Biological processi
- Notch signaling pathway Source: GO_Central
- protein ubiquitination Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Notch signaling pathway, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8N9I9 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable E3 ubiquitin-protein ligase DTX3 (EC:2.3.2.27By similarity)Alternative name(s): Protein deltex-3 Short name: Deltex3 RING finger protein 154 RING-type E3 ubiquitin transferase DTX3Curated |
Gene namesi | Name:DTX3 Synonyms:RNF154 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24457, DTX3 |
MIMi | 613142, gene |
neXtProti | NX_Q8N9I9 |
VEuPathDBi | HostDB:ENSG00000178498.15 |
Subcellular locationi
Other locations
- Cytoplasm Curated
Nucleus
- nucleoplasm Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 196403 |
OpenTargetsi | ENSG00000178498 |
PharmGKBi | PA134887936 |
Miscellaneous databases
Pharosi | Q8N9I9, Tbio |
Genetic variation databases
BioMutai | DTX3 |
DMDMi | 37077338 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219085 | 1 – 347 | Probable E3 ubiquitin-protein ligase DTX3Add BLAST | 347 |
Proteomic databases
MassIVEi | Q8N9I9 |
MaxQBi | Q8N9I9 |
PaxDbi | Q8N9I9 |
PeptideAtlasi | Q8N9I9 |
PRIDEi | Q8N9I9 |
ProteomicsDBi | 72545 [Q8N9I9-1] 72546 [Q8N9I9-2] |
TopDownProteomicsi | Q8N9I9-1 [Q8N9I9-1] |
PTM databases
iPTMneti | Q8N9I9 |
PhosphoSitePlusi | Q8N9I9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000178498, Expressed in right hemisphere of cerebellum and 211 other tissues |
ExpressionAtlasi | Q8N9I9, baseline and differential |
Genevisiblei | Q8N9I9, HS |
Organism-specific databases
HPAi | ENSG00000178498, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer. May form a heterodimers with other members of the Deltex family.
Interacts with NOTCH1.
By similarityBinary interactionsi
Hide detailsQ8N9I9
Protein-protein interaction databases
BioGRIDi | 128203, 52 interactors |
IntActi | Q8N9I9, 49 interactors |
STRINGi | 9606.ENSP00000448696 |
Miscellaneous databases
RNActi | Q8N9I9, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 122 – 151 | Pro-richAdd BLAST | 30 |
Sequence similaritiesi
Belongs to the Deltex family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 164 – 205 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QUYA, Eukaryota |
GeneTreei | ENSGT00940000161404 |
HOGENOMi | CLU_030422_1_0_1 |
InParanoidi | Q8N9I9 |
OMAi | REEHHVS |
OrthoDBi | 143182at2759 |
PhylomeDBi | Q8N9I9 |
TreeFami | TF325526 |
Family and domain databases
CDDi | cd09633, Deltex_C, 1 hit |
Gene3Di | 3.30.390.130, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR039396, Deltex_C IPR039399, Deltex_C_sf IPR039398, Deltex_fam IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR12622, PTHR12622, 1 hit |
Pfami | View protein in Pfam PF18102, DTC, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8N9I9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSFVLSRMAA CGGTCKNKVT VSKPVWDFLS KETPARLARL REEHRVSILI
60 70 80 90 100
DGETSDIYVL QLSPQGPPPA PPNGLYLARK ALKGLLKEAE KELKKAQRQG
110 120 130 140 150
ELMGCLALGG GGEHPEMHRA GPPPLRAAPL LPPGARGLPP PPPPLPPPLP
160 170 180 190 200
PRLREEAEEQ ESTCPICLGE IQNAKTLEKC RHSFCEGCIT RALQVKKACP
210 220 230 240 250
MCGRFYGQLV GNQPQNGRML VSKDATLLLP SYEKYGTIVI QYVFPPGVQG
260 270 280 290 300
AEHPNPGVRY PGTTRVAYLP DCPEGNKVLT LFRKAFDQRL TFTIGTSMTT
310 320 330 340
GRPNVITWND IHHKTSCTGG PQLFGYPDPT YLTRVQEELR AKGITDD
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VUZ4 | F8VUZ4_HUMAN | E3 ubiquitin-protein ligase | DTX3 | 204 | Annotation score: | ||
A0A0C4DGI8 | A0A0C4DGI8_HUMAN | E3 ubiquitin-protein ligase | DTX3 | 194 | Annotation score: | ||
H0YHF9 | H0YHF9_HUMAN | E3 ubiquitin-protein ligase | DTX3 | 172 | Annotation score: | ||
F8VV96 | F8VV96_HUMAN | Probable E3 ubiquitin-protein ligas... | DTX3 | 48 | Annotation score: | ||
A0A1Y8EH36 | A0A1Y8EH36_HUMAN | Probable E3 ubiquitin-protein ligas... | DTX3 | 7 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008354 | 1 – 7 | MSFVLSR → MPILSSSGSK in isoform 2. 1 Publication | 7 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY225126 mRNA Translation: AAP57520.1 AK092085 mRNA Translation: BAC03801.1 AK094385 mRNA Translation: BAC04344.1 AK128752 mRNA Translation: BAG54727.1 CH471054 Genomic DNA Translation: EAW97033.1 BC114441 mRNA Translation: AAI14442.1 BC114498 mRNA Translation: AAI14499.1 AL831941 mRNA Translation: CAD38593.2 Sequence problems. |
CCDSi | CCDS41800.1 [Q8N9I9-1] CCDS66410.1 [Q8N9I9-2] |
RefSeqi | NP_001273174.1, NM_001286245.1 [Q8N9I9-2] NP_001273175.1, NM_001286246.1 [Q8N9I9-1] NP_848597.1, NM_178502.3 [Q8N9I9-1] XP_005268754.1, XM_005268697.1 [Q8N9I9-2] XP_005268755.1, XM_005268698.1 [Q8N9I9-2] XP_005268757.1, XM_005268700.1 [Q8N9I9-2] XP_005268760.1, XM_005268703.1 [Q8N9I9-1] XP_011536324.1, XM_011538022.2 [Q8N9I9-1] |
Genome annotation databases
Ensembli | ENST00000337737; ENSP00000338050; ENSG00000178498 [Q8N9I9-1] ENST00000548198; ENSP00000447873; ENSG00000178498 [Q8N9I9-1] ENST00000548804; ENSP00000449294; ENSG00000178498 [Q8N9I9-1] ENST00000551632; ENSP00000448696; ENSG00000178498 [Q8N9I9-2] |
GeneIDi | 196403 |
KEGGi | hsa:196403 |
UCSCi | uc001sow.3, human [Q8N9I9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY225126 mRNA Translation: AAP57520.1 AK092085 mRNA Translation: BAC03801.1 AK094385 mRNA Translation: BAC04344.1 AK128752 mRNA Translation: BAG54727.1 CH471054 Genomic DNA Translation: EAW97033.1 BC114441 mRNA Translation: AAI14442.1 BC114498 mRNA Translation: AAI14499.1 AL831941 mRNA Translation: CAD38593.2 Sequence problems. |
CCDSi | CCDS41800.1 [Q8N9I9-1] CCDS66410.1 [Q8N9I9-2] |
RefSeqi | NP_001273174.1, NM_001286245.1 [Q8N9I9-2] NP_001273175.1, NM_001286246.1 [Q8N9I9-1] NP_848597.1, NM_178502.3 [Q8N9I9-1] XP_005268754.1, XM_005268697.1 [Q8N9I9-2] XP_005268755.1, XM_005268698.1 [Q8N9I9-2] XP_005268757.1, XM_005268700.1 [Q8N9I9-2] XP_005268760.1, XM_005268703.1 [Q8N9I9-1] XP_011536324.1, XM_011538022.2 [Q8N9I9-1] |
3D structure databases
SMRi | Q8N9I9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 128203, 52 interactors |
IntActi | Q8N9I9, 49 interactors |
STRINGi | 9606.ENSP00000448696 |
PTM databases
iPTMneti | Q8N9I9 |
PhosphoSitePlusi | Q8N9I9 |
Genetic variation databases
BioMutai | DTX3 |
DMDMi | 37077338 |
Proteomic databases
MassIVEi | Q8N9I9 |
MaxQBi | Q8N9I9 |
PaxDbi | Q8N9I9 |
PeptideAtlasi | Q8N9I9 |
PRIDEi | Q8N9I9 |
ProteomicsDBi | 72545 [Q8N9I9-1] 72546 [Q8N9I9-2] |
TopDownProteomicsi | Q8N9I9-1 [Q8N9I9-1] |
Protocols and materials databases
Antibodypediai | 28813, 152 antibodies |
Genome annotation databases
Ensembli | ENST00000337737; ENSP00000338050; ENSG00000178498 [Q8N9I9-1] ENST00000548198; ENSP00000447873; ENSG00000178498 [Q8N9I9-1] ENST00000548804; ENSP00000449294; ENSG00000178498 [Q8N9I9-1] ENST00000551632; ENSP00000448696; ENSG00000178498 [Q8N9I9-2] |
GeneIDi | 196403 |
KEGGi | hsa:196403 |
UCSCi | uc001sow.3, human [Q8N9I9-1] |
Organism-specific databases
CTDi | 196403 |
DisGeNETi | 196403 |
GeneCardsi | DTX3 |
HGNCi | HGNC:24457, DTX3 |
HPAi | ENSG00000178498, Low tissue specificity |
MIMi | 613142, gene |
neXtProti | NX_Q8N9I9 |
OpenTargetsi | ENSG00000178498 |
PharmGKBi | PA134887936 |
VEuPathDBi | HostDB:ENSG00000178498.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QUYA, Eukaryota |
GeneTreei | ENSGT00940000161404 |
HOGENOMi | CLU_030422_1_0_1 |
InParanoidi | Q8N9I9 |
OMAi | REEHHVS |
OrthoDBi | 143182at2759 |
PhylomeDBi | Q8N9I9 |
TreeFami | TF325526 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q8N9I9 |
Miscellaneous databases
BioGRID-ORCSi | 196403, 3 hits in 874 CRISPR screens |
GenomeRNAii | 196403 |
Pharosi | Q8N9I9, Tbio |
PROi | PR:Q8N9I9 |
RNActi | Q8N9I9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000178498, Expressed in right hemisphere of cerebellum and 211 other tissues |
ExpressionAtlasi | Q8N9I9, baseline and differential |
Genevisiblei | Q8N9I9, HS |
Family and domain databases
CDDi | cd09633, Deltex_C, 1 hit |
Gene3Di | 3.30.390.130, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR039396, Deltex_C IPR039399, Deltex_C_sf IPR039398, Deltex_fam IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR12622, PTHR12622, 1 hit |
Pfami | View protein in Pfam PF18102, DTC, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DTX3_HUMAN | |
Accessioni | Q8N9I9Primary (citable) accession number: Q8N9I9 Secondary accession number(s): Q53ZZ2, Q8NAU6, Q8NDS8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2003 |
Last sequence update: | September 26, 2003 | |
Last modified: | February 10, 2021 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families