UniProtKB - Q8N8Q3 (ENDOV_HUMAN)
Protein
Endonuclease V
Gene
ENDOV
Organism
Homo sapiens (Human)
Status
Functioni
Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed:23912683, PubMed:23912718, PubMed:27573237, PubMed:31703097, PubMed:25195743). Active against both single-stranded and double-stranded RNAs (PubMed:31703097, PubMed:25195743). Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs) (PubMed:23912718). Cleaves mRNAs and tRNAs containing inosine (PubMed:23912683, PubMed:31703097). Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3' (PubMed:23912718, PubMed:27573237). Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing (Probable). Binds branched DNA structures (PubMed:23139746).Curated6 Publications
Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed:31703097). Active against both single-stranded and double-stranded RNAs (PubMed:31703097). Cleaves tRNAs containing inosine (PubMed:31703097).1 Publication
Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine (PubMed:31703097). Active against both single-stranded and double-stranded RNAs (PubMed:31703097). Cleaves tRNAs containing inosine (PubMed:31703097).1 Publication
Caution
Was initially characterized as an endodeoxyribonuclease involved in DNA repair (PubMed:22664237). While it shows some weak endodeoxyribonuclease activity in vitro, such activity probably does not exist in vivo.1 Publication
Cofactori
Mg2+1 Publication
Activity regulationi
Inhibited by normal intracellular concentrations of ATP.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 52 | MagnesiumCurated | 1 | |
Metal bindingi | 126 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: UniProtKB
- endoribonuclease activity, producing 5'-phosphomonoesters Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- single-stranded RNA binding Source: UniProtKB
GO - Biological processi
- DNA repair Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, Endonuclease, Hydrolase, Nuclease, RNA-binding |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q8N8Q3 |
Names & Taxonomyi
Protein namesi | Recommended name: Endonuclease V (EC:3.1.26.-5 Publications)Short name: hEndoV Alternative name(s): Inosine-specific endoribonuclease |
Gene namesi | Name:ENDOV |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26640, ENDOV |
neXtProti | NX_Q8N8Q3 |
VEuPathDBi | HostDB:ENSG00000173818.16 |
Subcellular locationi
Nucleus
- nucleolus 2 Publications
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Other locations
- Stress granule 1 Publication
Note: Relocalizes to cytoplasmic stress granules upon cellular stress where it colocalizes with PABPC1.1 Publication
Nucleus
- nucleolus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- Stress granule 1 Publication
Note: Relocalizes to cytoplasmic stress granules upon cellular stress.1 Publication
Nucleus
- nucleolus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- Stress granule 1 Publication
Note: Relocalizes to cytoplasmic stress granules upon cellular stress.1 Publication
Nucleus
- nucleolus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic stress granule Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 35 – 40 | Missing : Does not gain activity against single- or double-stranded DNA. 1 Publication | 6 | |
Mutagenesisi | 52 | D → A: Abolishes ribonuclease activity. 3 Publications | 1 | |
Mutagenesisi | 57 – 60 | KGDS → QGGE: Does not gain activity against single- or double-stranded DNA. 1 Publication | 4 | |
Mutagenesisi | 90 – 93 | PYVS → GGGG: Abolishes ability to bind branched DNA and RNA. 1 Publication | 4 | |
Mutagenesisi | 91 | Y → A: Abolishes ribonuclease activity without affecting ability to bind branched DNA. 2 Publications | 1 | |
Mutagenesisi | 100 | E → A: Abolishes ribonuclease activity. 1 Publication | 1 | |
Mutagenesisi | 115 – 119 | Missing : Does not gain activity against single- or double-stranded DNA. 1 Publication | 5 | |
Mutagenesisi | 161 – 166 | Missing : Does not gain activity against single- or double-stranded DNA. 1 Publication | 6 | |
Mutagenesisi | 171 | E → A: No effect on subcellular location or activity; when associated with A-174. 1 Publication | 1 | |
Mutagenesisi | 174 | R → A: No effect on subcellular location or activity; when associated with A-171. 1 Publication | 1 | |
Mutagenesisi | 225 | C → S: No significant effect on activity. 1 Publication | 1 | |
Mutagenesisi | 226 | C → S: No significant effect on activity. 1 Publication | 1 | |
Mutagenesisi | 227 | C → S: 68% decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 228 | C → S: 46% decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 248 – 249 | RK → AA: Abolishes ability to bind branched DNA and RNA. 1 Publication | 2 |
Organism-specific databases
DisGeNETi | 284131 |
OpenTargetsi | ENSG00000173818 |
Miscellaneous databases
Pharosi | Q8N8Q3, Tbio |
Genetic variation databases
BioMutai | ENDOV |
DMDMi | 74729504 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000349223 | 1 – 282 | Endonuclease VAdd BLAST | 282 |
Proteomic databases
jPOSTi | Q8N8Q3 |
MassIVEi | Q8N8Q3 |
MaxQBi | Q8N8Q3 |
PaxDbi | Q8N8Q3 |
PeptideAtlasi | Q8N8Q3 |
PRIDEi | Q8N8Q3 |
ProteomicsDBi | 47312 72445 [Q8N8Q3-1] 72446 [Q8N8Q3-2] 72447 [Q8N8Q3-3] 72448 [Q8N8Q3-4] |
PTM databases
iPTMneti | Q8N8Q3 |
PhosphoSitePlusi | Q8N8Q3 |
Expressioni
Gene expression databases
Bgeei | ENSG00000173818, Expressed in quadriceps femoris and 192 other tissues |
ExpressionAtlasi | Q8N8Q3, baseline and differential |
Genevisiblei | Q8N8Q3, HS |
Organism-specific databases
HPAi | ENSG00000173818, Low tissue specificity |
Interactioni
Subunit structurei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 91 | Interaction with target RNACurated | 1 |
Protein-protein interaction databases
BioGRIDi | 129772, 12 interactors |
IntActi | Q8N8Q3, 9 interactors |
STRINGi | 9606.ENSP00000429190 |
Miscellaneous databases
RNActi | Q8N8Q3, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8N8Q3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 225 – 228 | Poly-Cys | 4 |
Sequence similaritiesi
Belongs to the endonuclease V family.Curated
Phylogenomic databases
eggNOGi | KOG4417, Eukaryota |
GeneTreei | ENSGT00390000011880 |
HOGENOMi | CLU_047631_0_1_1 |
InParanoidi | Q8N8Q3 |
OMAi | GIATHIG |
OrthoDBi | 1416056at2759 |
PhylomeDBi | Q8N8Q3 |
TreeFami | TF300065 |
Family and domain databases
CDDi | cd06559, Endonuclease_V, 1 hit |
HAMAPi | MF_00801, Endonuclease_5, 1 hit |
InterProi | View protein in InterPro IPR007581, Endonuclease-V |
PANTHERi | PTHR28511, PTHR28511, 1 hit |
Pfami | View protein in Pfam PF04493, Endonuclease_5, 1 hit |
s (7+)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 7 described isoforms and 14 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8N8Q3-1) [UniParc]FASTAAdd to basket
Also known as: hENDOV 2821 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALEAAGGPP EETLSLWKRE QARLKAHVVD RDTEAWQRDP AFSGLQRVGG
60 70 80 90 100
VDVSFVKGDS VRACASLVVL SFPELEVVYE ESRMVSLTAP YVSGFLAFRE
110 120 130 140 150
VPFLLELVQQ LREKEPGLMP QVLLVDGNGV LHHRGFGVAC HLGVLTDLPC
160 170 180 190 200
VGVAKKLLQV DGLENNALHK EKIRLLQTRG DSFPLLGDSG TVLGMALRSH
210 220 230 240 250
DRSTRPLYIS VGHRMSLEAA VRLTCCCCRF RIPEPVRQAD ICSREHIRKS
260 270 280
LGLPGPPTPR SPKAQRPVAC PKGDSGESSA LC
Computationally mapped potential isoform sequencesi
There are 14 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YAY5 | H0YAY5_HUMAN | Endonuclease V | ENDOV | 236 | Annotation score: | ||
H0YBZ5 | H0YBZ5_HUMAN | Endonuclease V | ENDOV | 170 | Annotation score: | ||
H0YBX8 | H0YBX8_HUMAN | Endonuclease V | ENDOV | 131 | Annotation score: | ||
I3L0L4 | I3L0L4_HUMAN | Endonuclease V | ENDOV | 115 | Annotation score: | ||
I3L4V7 | I3L4V7_HUMAN | Endonuclease V | ENDOV | 155 | Annotation score: | ||
E5RFW0 | E5RFW0_HUMAN | Endonuclease V | ENDOV | 105 | Annotation score: | ||
I3L485 | I3L485_HUMAN | Endonuclease V | ENDOV | 105 | Annotation score: | ||
E5RII5 | E5RII5_HUMAN | Endonuclease V | ENDOV | 138 | Annotation score: | ||
E5RJ92 | E5RJ92_HUMAN | Endonuclease V | ENDOV | 97 | Annotation score: | ||
E5RHX4 | E5RHX4_HUMAN | Endonuclease V | ENDOV | 50 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | R → W in BAC03912 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_046285 | 29 | V → I1 PublicationCorresponds to variant dbSNP:rs35549084Ensembl. | 1 | |
Natural variantiVAR_046286 | 112 | R → Q1 PublicationCorresponds to variant dbSNP:rs34933300Ensembl. | 1 | |
Natural variantiVAR_046287 | 114 | K → R1 PublicationCorresponds to variant dbSNP:rs41298706Ensembl. | 1 | |
Natural variantiVAR_046288 | 141 | H → Y1 PublicationCorresponds to variant dbSNP:rs41299812EnsemblClinVar. | 1 | |
Natural variantiVAR_046289 | 201 | D → N1 PublicationCorresponds to variant dbSNP:rs35929621Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_035228 | 1 – 194 | Missing in isoform 4 and isoform 5. 2 PublicationsAdd BLAST | 194 | |
Alternative sequenceiVSP_035229 | 77 – 121 | Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 45 | |
Alternative sequenceiVSP_035230 | 239 – 282 | ADICS…SSALC → HFVERGGESTRPRLIPDRTR W in isoform 4. 1 PublicationAdd BLAST | 44 | |
Alternative sequenceiVSP_035231 | 280 – 282 | ALC → GEGQPPQDHSPGPRTAPRPG SQEQAGKDWQ in isoform 3 and isoform 7. 1 Publication | 3 | |
Alternative sequenceiVSP_060583 | 280 – 282 | ALC → GGAPSPQRQADRTTPGGRRS TAQHQVGQR in isoform 6. | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK092539 mRNA Translation: BAC03912.1 AK096344 mRNA Translation: BAC04765.1 AK096802 mRNA No translation available. DQ500957 Genomic DNA Translation: ABF47100.1 AC120024 Genomic DNA No translation available. CH471099 Genomic DNA Translation: EAW89607.1 CH471099 Genomic DNA Translation: EAW89605.1 CH471099 Genomic DNA Translation: EAW89615.1 BC037889 mRNA Translation: AAH37889.1 BC045824 mRNA Translation: AAH45824.1 BC064545 mRNA Translation: AAH64545.1 |
CCDSi | CCDS54172.1 [Q8N8Q3-1] CCDS54173.1 [Q8N8Q3-2] CCDS54174.1 [Q8N8Q3-3] |
RefSeqi | NP_001158109.1, NM_001164637.2 [Q8N8Q3-2] NP_001158110.1, NM_001164638.1 [Q8N8Q3-3] NP_775898.2, NM_173627.4 [Q8N8Q3-1] XP_005257301.1, XM_005257244.3 |
Genome annotation databases
Ensembli | ENST00000323854; ENSP00000317810; ENSG00000173818 [Q8N8Q3-3] ENST00000517795; ENSP00000461577; ENSG00000173818 [Q8N8Q3-5] ENST00000518137; ENSP00000429190; ENSG00000173818 [Q8N8Q3-1] ENST00000518901; ENSP00000460685; ENSG00000173818 [Q8N8Q3-5] ENST00000518907; ENSP00000458361; ENSG00000173818 [Q8N8Q3-4] ENST00000520284; ENSP00000458391; ENSG00000173818 [Q8N8Q3-4] ENST00000520367; ENSP00000431036; ENSG00000173818 [Q8N8Q3-2] |
GeneIDi | 284131 |
KEGGi | hsa:284131 |
UCSCi | uc002jyk.4, human [Q8N8Q3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK092539 mRNA Translation: BAC03912.1 AK096344 mRNA Translation: BAC04765.1 AK096802 mRNA No translation available. DQ500957 Genomic DNA Translation: ABF47100.1 AC120024 Genomic DNA No translation available. CH471099 Genomic DNA Translation: EAW89607.1 CH471099 Genomic DNA Translation: EAW89605.1 CH471099 Genomic DNA Translation: EAW89615.1 BC037889 mRNA Translation: AAH37889.1 BC045824 mRNA Translation: AAH45824.1 BC064545 mRNA Translation: AAH64545.1 |
CCDSi | CCDS54172.1 [Q8N8Q3-1] CCDS54173.1 [Q8N8Q3-2] CCDS54174.1 [Q8N8Q3-3] |
RefSeqi | NP_001158109.1, NM_001164637.2 [Q8N8Q3-2] NP_001158110.1, NM_001164638.1 [Q8N8Q3-3] NP_775898.2, NM_173627.4 [Q8N8Q3-1] XP_005257301.1, XM_005257244.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4NSP | X-ray | 2.30 | A | 13-250 | [»] | |
6OZE | X-ray | 1.50 | A | 9-254 | [»] | |
SMRi | Q8N8Q3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 129772, 12 interactors |
IntActi | Q8N8Q3, 9 interactors |
STRINGi | 9606.ENSP00000429190 |
PTM databases
iPTMneti | Q8N8Q3 |
PhosphoSitePlusi | Q8N8Q3 |
Genetic variation databases
BioMutai | ENDOV |
DMDMi | 74729504 |
Proteomic databases
jPOSTi | Q8N8Q3 |
MassIVEi | Q8N8Q3 |
MaxQBi | Q8N8Q3 |
PaxDbi | Q8N8Q3 |
PeptideAtlasi | Q8N8Q3 |
PRIDEi | Q8N8Q3 |
ProteomicsDBi | 47312 72445 [Q8N8Q3-1] 72446 [Q8N8Q3-2] 72447 [Q8N8Q3-3] 72448 [Q8N8Q3-4] |
Protocols and materials databases
Antibodypediai | 32763, 28 antibodies |
DNASUi | 284131 |
Genome annotation databases
Organism-specific databases
CTDi | 284131 |
DisGeNETi | 284131 |
GeneCardsi | ENDOV |
HGNCi | HGNC:26640, ENDOV |
HPAi | ENSG00000173818, Low tissue specificity |
neXtProti | NX_Q8N8Q3 |
OpenTargetsi | ENSG00000173818 |
VEuPathDBi | HostDB:ENSG00000173818.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4417, Eukaryota |
GeneTreei | ENSGT00390000011880 |
HOGENOMi | CLU_047631_0_1_1 |
InParanoidi | Q8N8Q3 |
OMAi | GIATHIG |
OrthoDBi | 1416056at2759 |
PhylomeDBi | Q8N8Q3 |
TreeFami | TF300065 |
Enzyme and pathway databases
PathwayCommonsi | Q8N8Q3 |
Miscellaneous databases
BioGRID-ORCSi | 284131, 6 hits in 987 CRISPR screens |
GenomeRNAii | 284131 |
Pharosi | Q8N8Q3, Tbio |
PROi | PR:Q8N8Q3 |
RNActi | Q8N8Q3, protein |
Gene expression databases
Bgeei | ENSG00000173818, Expressed in quadriceps femoris and 192 other tissues |
ExpressionAtlasi | Q8N8Q3, baseline and differential |
Genevisiblei | Q8N8Q3, HS |
Family and domain databases
CDDi | cd06559, Endonuclease_V, 1 hit |
HAMAPi | MF_00801, Endonuclease_5, 1 hit |
InterProi | View protein in InterPro IPR007581, Endonuclease-V |
PANTHERi | PTHR28511, PTHR28511, 1 hit |
Pfami | View protein in Pfam PF04493, Endonuclease_5, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ENDOV_HUMAN | |
Accessioni | Q8N8Q3Primary (citable) accession number: Q8N8Q3 Secondary accession number(s): I3L3S4 Q8NAK0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 2, 2008 |
Last sequence update: | October 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families