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UniProtKB - Q8N8Q3 (ENDOV_HUMAN)

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Protein

Endonuclease V

Gene

ENDOV

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures.3 Publications

Miscellaneous

Was initially characterized as an endodeoxyribonuclease involved in DNA repair (PubMed:22664237). While it shows some weak endodeoxyribonuclease activity in vitro, such activity probably does not exist in vivo.1 Publication

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52MagnesiumCurated1
Metal bindingi126MagnesiumBy similarity1

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: UniProtKB
  • endoribonuclease activity, producing 5'-phosphomonoesters Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • single-stranded RNA binding Source: UniProtKB
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease, RNA-binding
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease V (EC:3.1.26.-)
Short name:
hEndoV
Alternative name(s):
Inosine-specific endoribonuclease
Gene namesi
Name:ENDOV
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000173818.16
HGNCiHGNC:26640 ENDOV
neXtProtiNX_Q8N8Q3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52D → A: Abolishes ribonuclease activity. 2 Publications1
Mutagenesisi90 – 93PYVS → GGGG: Abolishes ability to bind branched DNA and RNA. 1 Publication4
Mutagenesisi91Y → A: Abolishes ribonuclease activity without affecting ability to bind branched DNA. 2 Publications1
Mutagenesisi100E → A: Abolishes ribonuclease activity. 1 Publication1
Mutagenesisi248 – 249RK → AA: Abolishes ability to bind branched DNA and RNA. 1 Publication2

Organism-specific databases

DisGeNETi284131
OpenTargetsiENSG00000173818

Polymorphism and mutation databases

BioMutaiENDOV
DMDMi74729504

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003492231 – 282Endonuclease VAdd BLAST282

Proteomic databases

MaxQBiQ8N8Q3
PaxDbiQ8N8Q3
PeptideAtlasiQ8N8Q3
PRIDEiQ8N8Q3
ProteomicsDBi72445
72446 [Q8N8Q3-2]
72447 [Q8N8Q3-3]
72448 [Q8N8Q3-4]

PTM databases

iPTMnetiQ8N8Q3
PhosphoSitePlusiQ8N8Q3

Expressioni

Gene expression databases

BgeeiENSG00000173818
ExpressionAtlasiQ8N8Q3 baseline and differential
GenevisibleiQ8N8Q3 HS

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei91Interaction with target RNACurated1

Protein-protein interaction databases

BioGridi129772, 11 interactors
IntActiQ8N8Q3, 2 interactors
STRINGi9606.ENSP00000429190

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 25Combined sources11
Helixi35 – 38Combined sources4
Beta strandi47 – 55Combined sources9
Beta strandi61 – 71Combined sources11
Turni72 – 74Combined sources3
Beta strandi77 – 86Combined sources10
Helixi97 – 114Combined sources18
Helixi116 – 118Combined sources3
Beta strandi121 – 127Combined sources7
Beta strandi129 – 132Combined sources4
Helixi138 – 146Combined sources9
Beta strandi150 – 156Combined sources7
Helixi167 – 175Combined sources9
Beta strandi182 – 186Combined sources5
Beta strandi192 – 197Combined sources6
Beta strandi207 – 215Combined sources9
Helixi217 – 226Combined sources10
Beta strandi229 – 232Combined sources4
Helixi234 – 250Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NSPX-ray2.30A13-250[»]
ProteinModelPortaliQ8N8Q3
SMRiQ8N8Q3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi225 – 228Poly-Cys4

Sequence similaritiesi

Belongs to the endonuclease V family.Curated

Phylogenomic databases

eggNOGiKOG4417 Eukaryota
COG1515 LUCA
GeneTreeiENSGT00390000011880
HOVERGENiHBG062398
InParanoidiQ8N8Q3
KOiK21813
OMAiCKYRVPE
OrthoDBiEOG091G0LHO
PhylomeDBiQ8N8Q3
TreeFamiTF300065

Family and domain databases

CDDicd06559 Endonuclease_V, 1 hit
HAMAPiMF_00801 Endonuclease_5, 1 hit
InterProiView protein in InterPro
IPR007581 Endonuclease-V
PANTHERiPTHR28511 PTHR28511, 1 hit
PfamiView protein in Pfam
PF04493 Endonuclease_5, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N8Q3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALEAAGGPP EETLSLWKRE QARLKAHVVD RDTEAWQRDP AFSGLQRVGG 
        60         70         80         90        100
VDVSFVKGDS VRACASLVVL SFPELEVVYE ESRMVSLTAP YVSGFLAFRE 
       110        120        130        140        150
VPFLLELVQQ LREKEPGLMP QVLLVDGNGV LHHRGFGVAC HLGVLTDLPC 
       160        170        180        190        200
VGVAKKLLQV DGLENNALHK EKIRLLQTRG DSFPLLGDSG TVLGMALRSH 
       210        220        230        240        250
DRSTRPLYIS VGHRMSLEAA VRLTCCCCRF RIPEPVRQAD ICSREHIRKS 
       260        270        280 
LGLPGPPTPR SPKAQRPVAC PKGDSGESSA LC
Length:282
Mass (Da):30,792
Last modified:October 1, 2002 - v1
Checksum:iEDB10210FB508A51
GO
Isoform 2 (identifier: Q8N8Q3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-121: Missing.

Show »
Length:237
Mass (Da):25,612
Checksum:iA89BC40B3E5E0D22
GO
Isoform 3 (identifier: Q8N8Q3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-121: Missing.
     280-282: ALC → GEGQPPQDHSPGPRTAPRPGSQEQAGKDWQ

Show »
Length:264
Mass (Da):28,503
Checksum:iEA8145ACC6AB8CF9
GO
Isoform 4 (identifier: Q8N8Q3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-194: Missing.
     239-282: ADICSREHIRKSLGLPGPPTPRSPKAQRPVACPKGDSGESSALC → HFVERGGESTRPRLIPDRTRW

Show »
Length:65
Mass (Da):7,665
Checksum:iE75AB2F40226FC4E
GO
Isoform 5 (identifier: Q8N8Q3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-194: Missing.

Show »
Length:88
Mass (Da):9,670
Checksum:i77BE0951D5518E2F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19R → W in BAC03912 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04628529V → I1 PublicationCorresponds to variant dbSNP:rs35549084Ensembl.1
Natural variantiVAR_046286112R → Q1 PublicationCorresponds to variant dbSNP:rs34933300Ensembl.1
Natural variantiVAR_046287114K → R1 PublicationCorresponds to variant dbSNP:rs41298706Ensembl.1
Natural variantiVAR_046288141H → Y1 PublicationCorresponds to variant dbSNP:rs41299812Ensembl.1
Natural variantiVAR_046289201D → N1 PublicationCorresponds to variant dbSNP:rs35929621Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0352281 – 194Missing in isoform 4 and isoform 5. 2 PublicationsAdd BLAST194
Alternative sequenceiVSP_03522977 – 121Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST45
Alternative sequenceiVSP_035230239 – 282ADICS…SSALC → HFVERGGESTRPRLIPDRTR W in isoform 4. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_035231280 – 282ALC → GEGQPPQDHSPGPRTAPRPG SQEQAGKDWQ in isoform 3. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK092539 mRNA Translation: BAC03912.1
AK096344 mRNA Translation: BAC04765.1
AK096802 mRNA No translation available.
DQ500957 Genomic DNA Translation: ABF47100.1
AC120024 Genomic DNA No translation available.
CH471099 Genomic DNA Translation: EAW89607.1
CH471099 Genomic DNA Translation: EAW89605.1
CH471099 Genomic DNA Translation: EAW89615.1
BC037889 mRNA Translation: AAH37889.1
BC045824 mRNA Translation: AAH45824.1
BC064545 mRNA Translation: AAH64545.1
CCDSiCCDS54172.1 [Q8N8Q3-1]
CCDS54173.1 [Q8N8Q3-2]
CCDS54174.1 [Q8N8Q3-3]
RefSeqiNP_001158109.1, NM_001164637.2 [Q8N8Q3-2]
NP_001158110.1, NM_001164638.1 [Q8N8Q3-3]
NP_775898.2, NM_173627.4 [Q8N8Q3-1]
UniGeneiHs.389678
Hs.728933

Genome annotation databases

EnsembliENST00000323854; ENSP00000317810; ENSG00000173818 [Q8N8Q3-3]
ENST00000517795; ENSP00000461577; ENSG00000173818 [Q8N8Q3-5]
ENST00000518137; ENSP00000429190; ENSG00000173818 [Q8N8Q3-1]
ENST00000518901; ENSP00000460685; ENSG00000173818 [Q8N8Q3-5]
ENST00000518907; ENSP00000458361; ENSG00000173818 [Q8N8Q3-4]
ENST00000520284; ENSP00000458391; ENSG00000173818 [Q8N8Q3-4]
ENST00000520367; ENSP00000431036; ENSG00000173818 [Q8N8Q3-2]
GeneIDi284131
KEGGihsa:284131
UCSCiuc002jyk.4 human [Q8N8Q3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiENDOV_HUMAN
AccessioniPrimary (citable) accession number: Q8N8Q3
Secondary accession number(s): I3L3S4
, Q6P2G2, Q86X99, Q8NAK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

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