UniProtKB - Q8N7E2 (CBLL2_HUMAN)
Protein
E3 ubiquitin-protein ligase CBLL2
Gene
CBLL2
Organism
Homo sapiens (Human)
Status
Functioni
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed:20657603). May operate on tyrosine-phosphorylated SRC substrates (PubMed:22252131).2 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 57 – 97 | RING-typePROSITE-ProRule annotationAdd BLAST | 41 | |
Zinc fingeri | 112 – 138 | C2H2-typePROSITE-ProRule annotationAdd BLAST | 27 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: GO_Central
GO - Biological processi
- protein ubiquitination Source: GO_Central
- regulation of cell adhesion Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8N7E2 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase CBLL2Curated (EC:2.3.2.271 Publication)Alternative name(s): Cbl proto-oncogene-like protein 2 RING-type E3 ubiquitin transferase ZNF645 Zinc finger protein 645 c-Cbl-like protein 2 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000175809.5 |
HGNCi | HGNC:26371, CBLL2 |
neXtProti | NX_Q8N7E2 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 158506 |
OpenTargetsi | ENSG00000175809 |
PharmGKBi | PA134934025 |
Miscellaneous databases
Pharosi | Q8N7E2, Tdark |
Polymorphism and mutation databases
BioMutai | ZNF645 |
DMDMi | 74759980 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056316 | 1 – 425 | E3 ubiquitin-protein ligase CBLL2Add BLAST | 425 |
Proteomic databases
PaxDbi | Q8N7E2 |
PRIDEi | Q8N7E2 |
ProteomicsDBi | 72291 |
PTM databases
iPTMneti | Q8N7E2 |
PhosphoSitePlusi | Q8N7E2 |
Expressioni
Tissue specificityi
Exclusively expressed in testis and sperm, including spermatocytes, round and elongated spermatids, and Leydig cells.1 Publication
Gene expression databases
Bgeei | ENSG00000175809, Expressed in sperm and 9 other tissues |
Genevisiblei | Q8N7E2, HS |
Organism-specific databases
HPAi | ENSG00000175809, Tissue enriched (testis) |
Interactioni
Subunit structurei
Homodimer.
By similarityBinary interactionsi
Hide detailsQ8N7E2
Protein-protein interaction databases
BioGRIDi | 127688, 13 interactors |
IntActi | Q8N7E2, 13 interactors |
STRINGi | 9606.ENSP00000323348 |
Miscellaneous databases
RNActi | Q8N7E2, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 96 – 154 | HYB domainAdd BLAST | 59 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 254 – 343 | Pro-richAdd BLAST | 90 |
Domaini
The HYB domain forms a phosphotyrosine-binding pocket upon dimerization, and mediates as well the recognition of its flanking acidic amino acids.By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 57 – 97 | RING-typePROSITE-ProRule annotationAdd BLAST | 41 | |
Zinc fingeri | 112 – 138 | C2H2-typePROSITE-ProRule annotationAdd BLAST | 27 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2932, Eukaryota |
GeneTreei | ENSGT00510000047522 |
HOGENOMi | CLU_031291_1_0_1 |
InParanoidi | Q8N7E2 |
OMAi | FPIQWET |
OrthoDBi | 765364at2759 |
PhylomeDBi | Q8N7E2 |
TreeFami | TF332910 |
Family and domain databases
CDDi | cd16508, RING-HC_HAKAI_like, 1 hit |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR040380, HAKAI-like_RING-HC IPR040383, HAKAI/CBLL2 IPR013087, Znf_C2H2_type IPR041042, Znf_Hakai IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR13480, PTHR13480, 1 hit |
Pfami | View protein in Pfam PF18408, zf_Hakai, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit PS50157, ZINC_FINGER_C2H2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8N7E2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKMPAGEQE CEYNKEGKYY SKGVKLVRKK KKIPGYRWGD IKINIIGEKD
60 70 80 90 100
DLPIHFCDKC DLPIKIYGRI IPCKHAFCYH CANLYDKVGY KVCPRCRYPV
110 120 130 140 150
LRIEAHKRGS VFMCSIVQQC KRTYLSQKSL QAHIKRRHKR ARKQVTSASL
160 170 180 190 200
EKVRPHIAPP QTEISDIPKR LQDRDHLSYI PPEQHTMVSL PSVQHMLQEQ
210 220 230 240 250
HNQPHKDIQA PPPELSLSLP FPIQWETVSI FTRKHGNLTV DHIQNNSDSG
260 270 280 290 300
AKKPTPPDYY PECQSQPAVS SPHHIIPQKQ HYAPPPSPSS PVNHQMPYPP
310 320 330 340 350
QDVVTPNSVR SQVPALTTTY DPSSGYIIVK VPPDMNSPPL RAPQSQNGNP
360 370 380 390 400
SASEFASHHY NLNILPQFTE NQETLSPQFT QTDAMDHRRW PAWKRLSPCP
410 420
PTRSPPPSTL HGRSHHSHQR RHRRY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 267 | P → L in ADI56589 (PubMed:20657603).Curated | 1 | |
Sequence conflicti | 411 | H → Q in ADI56589 (PubMed:20657603).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_030340 | 166 | D → E2 PublicationsCorresponds to variant dbSNP:rs5951426Ensembl. | 1 | |
Natural variantiVAR_030341 | 287 | S → F. Corresponds to variant dbSNP:rs12860105Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GQ355336 mRNA Translation: ADI56589.1 AK098601 mRNA Translation: BAC05348.1 BX293560 Genomic DNA No translation available. BC074910 mRNA Translation: AAH74910.1 BC126190 mRNA Translation: AAI26191.1 BC126192 mRNA Translation: AAI26193.1 |
CCDSi | CCDS14205.1 |
RefSeqi | NP_689790.1, NM_152577.3 |
Genome annotation databases
Ensembli | ENST00000323684; ENSP00000323348; ENSG00000175809 |
GeneIDi | 158506 |
KEGGi | hsa:158506 |
UCSCi | uc004dai.3, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GQ355336 mRNA Translation: ADI56589.1 AK098601 mRNA Translation: BAC05348.1 BX293560 Genomic DNA No translation available. BC074910 mRNA Translation: AAH74910.1 BC126190 mRNA Translation: AAI26191.1 BC126192 mRNA Translation: AAI26193.1 |
CCDSi | CCDS14205.1 |
RefSeqi | NP_689790.1, NM_152577.3 |
3D structure databases
SMRi | Q8N7E2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 127688, 13 interactors |
IntActi | Q8N7E2, 13 interactors |
STRINGi | 9606.ENSP00000323348 |
PTM databases
iPTMneti | Q8N7E2 |
PhosphoSitePlusi | Q8N7E2 |
Polymorphism and mutation databases
BioMutai | ZNF645 |
DMDMi | 74759980 |
Proteomic databases
PaxDbi | Q8N7E2 |
PRIDEi | Q8N7E2 |
ProteomicsDBi | 72291 |
Protocols and materials databases
Antibodypediai | 399, 79 antibodies |
DNASUi | 158506 |
Genome annotation databases
Ensembli | ENST00000323684; ENSP00000323348; ENSG00000175809 |
GeneIDi | 158506 |
KEGGi | hsa:158506 |
UCSCi | uc004dai.3, human |
Organism-specific databases
CTDi | 158506 |
DisGeNETi | 158506 |
EuPathDBi | HostDB:ENSG00000175809.5 |
GeneCardsi | CBLL2 |
HGNCi | HGNC:26371, CBLL2 |
HPAi | ENSG00000175809, Tissue enriched (testis) |
neXtProti | NX_Q8N7E2 |
OpenTargetsi | ENSG00000175809 |
PharmGKBi | PA134934025 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2932, Eukaryota |
GeneTreei | ENSGT00510000047522 |
HOGENOMi | CLU_031291_1_0_1 |
InParanoidi | Q8N7E2 |
OMAi | FPIQWET |
OrthoDBi | 765364at2759 |
PhylomeDBi | Q8N7E2 |
TreeFami | TF332910 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q8N7E2 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 158506, 3 hits in 474 CRISPR screens |
GenomeRNAii | 158506 |
Pharosi | Q8N7E2, Tdark |
PROi | PR:Q8N7E2 |
RNActi | Q8N7E2, protein |
Gene expression databases
Bgeei | ENSG00000175809, Expressed in sperm and 9 other tissues |
Genevisiblei | Q8N7E2, HS |
Family and domain databases
CDDi | cd16508, RING-HC_HAKAI_like, 1 hit |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR040380, HAKAI-like_RING-HC IPR040383, HAKAI/CBLL2 IPR013087, Znf_C2H2_type IPR041042, Znf_Hakai IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR13480, PTHR13480, 1 hit |
Pfami | View protein in Pfam PF18408, zf_Hakai, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit PS50157, ZINC_FINGER_C2H2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CBLL2_HUMAN | |
Accessioni | Q8N7E2Primary (citable) accession number: Q8N7E2 Secondary accession number(s): A0AV29 Q6DJY9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - PATHWAY comments
Index of metabolic and biosynthesis pathways - Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations