UniProtKB - Q8N6Q3 (CD177_HUMAN)
CD177 antigen
CD177
Functioni
In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407).
In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980).
Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308).
However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681).
Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208).
By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).
7 PublicationsGO - Molecular functioni
- calcium-dependent protein binding Source: UniProtKB
- integrin binding Source: UniProtKB
- protease binding Source: UniProtKB
GO - Biological processi
- cell adhesion Source: UniProtKB
- cell-cell adhesion via plasma-membrane adhesion molecules Source: UniProtKB
- cell-cell junction maintenance Source: UniProtKB
- innate immune response Source: UniProtKB-KW
- leukocyte cell-cell adhesion Source: UniProtKB
- neutrophil extravasation Source: UniProtKB
- neutrophil migration Source: UniProtKB
- positive regulation of neutrophil degranulation Source: UniProtKB
- positive regulation of superoxide anion generation Source: UniProtKB
- protein localization to cell surface Source: UniProtKB
- regulation of endocytosis Source: UniProtKB
- regulation of integrin-mediated signaling pathway Source: UniProtKB
Keywordsi
Biological process | Cell adhesion, Immunity, Innate immunity |
Enzyme and pathway databases
PathwayCommonsi | Q8N6Q3 |
Reactomei | R-HSA-140875, Common Pathway of Fibrin Clot Formation R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-6798695, Neutrophil degranulation |
SignaLinki | Q8N6Q3 |
Names & Taxonomyi
Protein namesi | Recommended name: CD177 antigenImportedAlternative name(s): Human neutrophil alloantigen 2a1 Publication Short name: HNA-2a1 Publication NB1 glycoprotein1 Publication Short name: NB1 GP1 Publication Polycythemia rubra vera protein 11 Publication Short name: PRV-11 Publication CD_antigen: CD177 |
Gene namesi | ORF Names:UNQ595/PRO1181 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30072, CD177 |
MIMi | 162860, gene |
neXtProti | NX_Q8N6Q3 |
VEuPathDBi | HostDB:ENSG00000204936 |
Subcellular locationi
Plasma membrane
- Cell membrane 10 Publications; GPI-anchor 2 Publications
Extracellular region or secreted
- Secreted 2 Publications
Other locations
- Membrane raft 1 Publication; GPI-like-anchor 2 Publications
- Cytoplasmic granule membrane 1 Publication
- lamellipodium 1 Publication
Note: Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308). In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208). Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208). Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980).5 Publications
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- anchored component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
- plasma membrane raft Source: UniProtKB
Other locations
- lamellipodium Source: UniProtKB-SubCell
- secretory granule membrane Source: UniProtKB
- specific granule membrane Source: Reactome
- tertiary granule membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Membrane, SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 57126 |
OpenTargetsi | ENSG00000204936 |
PharmGKBi | PA142672147 |
Miscellaneous databases
Pharosi | Q8N6Q3, Tbio |
Genetic variation databases
BioMutai | CD177 |
DMDMi | 91208246 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | 1 PublicationAdd BLAST | 21 | |
ChainiPRO_0000231643 | 22 – 408 | CD177 antigenAdd BLAST | 387 | |
PropeptideiPRO_0000231644 | 409 – 437 | Removed in mature formSequence analysisAdd BLAST | 29 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 189 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 408 | GPI-anchor amidated glycineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, GPI-anchor, LipoproteinProteomic databases
jPOSTi | Q8N6Q3 |
MassIVEi | Q8N6Q3 |
PaxDbi | Q8N6Q3 |
PeptideAtlasi | Q8N6Q3 |
PRIDEi | Q8N6Q3 |
ProteomicsDBi | 72212 [Q8N6Q3-1] 72213 [Q8N6Q3-2] 72214 [Q8N6Q3-3] |
PTM databases
GlyGeni | Q8N6Q3, 1 site |
PhosphoSitePlusi | Q8N6Q3 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSG00000204936, Expressed in blood and 129 other tissues |
ExpressionAtlasi | Q8N6Q3, baseline and differential |
Genevisiblei | Q8N6Q3, HS |
Interactioni
Subunit structurei
Found in a complex with integrin ITGAM/CD11b and ITGB2/CD18 (PubMed:28807980, PubMed:21193407).
Interacts with PECAM1 (via Ig-like C2-type domain 6); the interaction is Ca2+-dependent; the interaction is direct (PubMed:17580308).
Interacts with serine protease PRTN3/myeloblastin; the interaction tethers PRTN3 to the cell surface; the interaction is direct (PubMed:17244676, PubMed:28240246).
5 PublicationsBinary interactionsi
Q8N6Q3
With | #Exp. | IntAct |
---|---|---|
KCNIP3 [Q9Y2W7] | 9 | EBI-747170,EBI-751501 |
GO - Molecular functioni
- calcium-dependent protein binding Source: UniProtKB
- integrin binding Source: UniProtKB
- protease binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 121389, 4 interactors |
IntActi | Q8N6Q3, 100 interactors |
STRINGi | 9606.ENSP00000479536 |
Miscellaneous databases
RNActi | Q8N6Q3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 133 – 210 | UPAR/Ly6 1Add BLAST | 78 | |
Domaini | 325 – 377 | UPAR/Ly6 2Add BLAST | 53 |
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502ST5V, Eukaryota |
GeneTreei | ENSGT00530000063351 |
InParanoidi | Q8N6Q3 |
OrthoDBi | 1103796at2759 |
PhylomeDBi | Q8N6Q3 |
Family and domain databases
InterProi | View protein in InterPro IPR016054, LY6_UPA_recep-like IPR045860, Snake_toxin-like_sf |
Pfami | View protein in Pfam PF00021, UPAR_LY6, 2 hits |
SUPFAMi | SSF57302, SSF57302, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSAVLLLALL GFILPLPGVQ ALLCQFGTVQ HVWKVSDLPR QWTPKNTSCD
60 70 80 90 100
SGLGCQDTLM LIESGPQVSL VLSKGCTEAK DQEPRVTEHR MGPGLSLISY
110 120 130 140 150
TFVCRQEDFC NNLVNSLPLW APQPPADPGS LRCPVCLSME GCLEGTTEEI
160 170 180 190 200
CPKGTTHCYD GLLRLRGGGI FSNLRVQGCM PQPGCNLLNG TQEIGPVGMT
210 220 230 240 250
ENCNRKDFLT CHRGTTIMTH GNLAQEPTDW TTSNTEMCEV GQVCQETLLL
260 270 280 290 300
LDVGLTSTLV GTKGCSTVGA QNSQKTTIHS APPGVLVASY THFCSSDLCN
310 320 330 340 350
SASSSSVLLN SLPPQAAPVP GDRQCPTCVQ PLGTCSSGSP RMTCPRGATH
360 370 380 390 400
CYDGYIHLSG GGLSTKMSIQ GCVAQPSSFL LNHTRQIGIF SAREKRDVQP
410 420 430
PASQHEGGGA EGLESLTWGV GLALAPALWW GVVCPSC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WVM2 | A0A087WVM2_HUMAN | CD177 antigen | CD177 | 437 | Annotation score: | ||
A0A087X113 | A0A087X113_HUMAN | CD177 antigen | CD177 | 139 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 89 | H → Y in CAC83724 (PubMed:12010833).Curated | 1 | |
Sequence conflicti | 184 | G → V in CAC83724 (PubMed:12010833).Curated | 1 | |
Sequence conflicti | 194 | I → F in CAC83724 (PubMed:12010833).Curated | 1 | |
Sequence conflicti | 204 – 205 | NR → DM in CAC83724 (PubMed:12010833).Curated | 2 |
Mass spectrometryi
Polymorphismi
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_025858 | 3 | A → P5 PublicationsCorresponds to variant dbSNP:rs45441892Ensembl. | 1 | |
Natural variantiVAR_079754 | 31 | H → L1 PublicationCorresponds to variant dbSNP:rs45553433Ensembl. | 1 | |
Natural variantiVAR_026156 | 119 | L → F2 Publications | 1 | |
Natural variantiVAR_025859 | 251 | L → I5 PublicationsCorresponds to variant dbSNP:rs10425835Ensembl. | 1 | |
Natural variantiVAR_079755 | 261 | G → A1 Publication | 1 | |
Natural variantiVAR_026157 | 323 | R → Q2 Publications | 1 | |
Natural variantiVAR_025860 | 348 | A → T3 PublicationsCorresponds to variant dbSNP:rs17856829Ensembl. | 1 | |
Natural variantiVAR_026158 | 379 | F → S2 Publications | 1 | |
Natural variantiVAR_079756 | 431 | G → R1 PublicationCorresponds to variant dbSNP:rs78718189Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017858 | 129 – 145 | GSLRC…GCLEG → MMGAAEGPFGKAEQVDS in isoform 3. 1 PublicationAdd BLAST | 17 | |
Alternative sequenceiVSP_017859 | 146 – 437 | Missing in isoform 3. 1 PublicationAdd BLAST | 292 | |
Alternative sequenceiVSP_017860 | 210 – 248 | TCHRG…CQETL → QLKPVGLLEEHNVGEKERGQ GQHQCVQLDWQKRAATGIF in isoform 2. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_017861 | 249 – 437 | Missing in isoform 2. 1 PublicationAdd BLAST | 189 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF146747 mRNA Translation: AAG00895.1 AJ290452 mRNA Translation: CAC44459.1 AJ305326 mRNA Translation: CAC83758.1 AJ310433 mRNA Translation: CAC83724.1 AY358932 mRNA Translation: AAQ89291.1 BT020111 mRNA Translation: AAV38914.1 BC029167 mRNA Translation: AAH29167.1 |
CCDSi | CCDS62700.1 [Q8N6Q3-1] |
RefSeqi | NP_065139.2, NM_020406.3 |
Genome annotation databases
Ensembli | ENST00000378012; ENSP00000367251; ENSG00000204936 [Q8N6Q3-3] |
GeneIDi | 57126 |
KEGGi | hsa:57126 |
UCSCi | uc060zla.1, human [Q8N6Q3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF146747 mRNA Translation: AAG00895.1 AJ290452 mRNA Translation: CAC44459.1 AJ305326 mRNA Translation: CAC83758.1 AJ310433 mRNA Translation: CAC83724.1 AY358932 mRNA Translation: AAQ89291.1 BT020111 mRNA Translation: AAV38914.1 BC029167 mRNA Translation: AAH29167.1 |
CCDSi | CCDS62700.1 [Q8N6Q3-1] |
RefSeqi | NP_065139.2, NM_020406.3 |
3D structure databases
SMRi | Q8N6Q3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 121389, 4 interactors |
IntActi | Q8N6Q3, 100 interactors |
STRINGi | 9606.ENSP00000479536 |
PTM databases
GlyGeni | Q8N6Q3, 1 site |
PhosphoSitePlusi | Q8N6Q3 |
Genetic variation databases
BioMutai | CD177 |
DMDMi | 91208246 |
Proteomic databases
jPOSTi | Q8N6Q3 |
MassIVEi | Q8N6Q3 |
PaxDbi | Q8N6Q3 |
PeptideAtlasi | Q8N6Q3 |
PRIDEi | Q8N6Q3 |
ProteomicsDBi | 72212 [Q8N6Q3-1] 72213 [Q8N6Q3-2] 72214 [Q8N6Q3-3] |
Protocols and materials databases
DNASUi | 57126 |
Genome annotation databases
Ensembli | ENST00000378012; ENSP00000367251; ENSG00000204936 [Q8N6Q3-3] |
GeneIDi | 57126 |
KEGGi | hsa:57126 |
UCSCi | uc060zla.1, human [Q8N6Q3-1] |
Organism-specific databases
CTDi | 57126 |
DisGeNETi | 57126 |
GeneCardsi | CD177 |
HGNCi | HGNC:30072, CD177 |
MIMi | 162860, gene |
neXtProti | NX_Q8N6Q3 |
OpenTargetsi | ENSG00000204936 |
PharmGKBi | PA142672147 |
VEuPathDBi | HostDB:ENSG00000204936 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502ST5V, Eukaryota |
GeneTreei | ENSGT00530000063351 |
InParanoidi | Q8N6Q3 |
OrthoDBi | 1103796at2759 |
PhylomeDBi | Q8N6Q3 |
Enzyme and pathway databases
PathwayCommonsi | Q8N6Q3 |
Reactomei | R-HSA-140875, Common Pathway of Fibrin Clot Formation R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-6798695, Neutrophil degranulation |
SignaLinki | Q8N6Q3 |
Miscellaneous databases
BioGRID-ORCSi | 57126, 11 hits in 613 CRISPR screens |
GeneWikii | CD177 |
GenomeRNAii | 57126 |
Pharosi | Q8N6Q3, Tbio |
PROi | PR:Q8N6Q3 |
RNActi | Q8N6Q3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204936, Expressed in blood and 129 other tissues |
ExpressionAtlasi | Q8N6Q3, baseline and differential |
Genevisiblei | Q8N6Q3, HS |
Family and domain databases
InterProi | View protein in InterPro IPR016054, LY6_UPA_recep-like IPR045860, Snake_toxin-like_sf |
Pfami | View protein in Pfam PF00021, UPAR_LY6, 2 hits |
SUPFAMi | SSF57302, SSF57302, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CD177_HUMAN | |
Accessioni | Q8N6Q3Primary (citable) accession number: Q8N6Q3 Secondary accession number(s): Q711Q2 Q9HDA5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 4, 2006 |
Last sequence update: | April 4, 2006 | |
Last modified: | February 23, 2022 | |
This is version 132 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot