UniProtKB - Q8N6M6 (AMPO_HUMAN)
Protein
Aminopeptidase O
Gene
AOPEP
Organism
Homo sapiens (Human)
Status
Functioni
Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart.1 Publication
Caution
The article describing the function, tissue specificity, activity regulation and biophysicochemical properties has been retracted due to concerns of image manipulation but the authors stand by the validity of the main results and conclusions.1 Publication
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Activity regulationi
Strongly inhibited by ophenanthroline, a metalloprotease inhibitor. Inhibited by arphamenine A, a potent inhibitor of aminopeptidases. Not affected by AEBSF and E-64, which are common inhibitors of serine- and cysteine-proteases, respectively.1 Publication
Kineticsi
- KM=6 µM for Arg-AMC1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 479 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 480 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 483 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 502 | Zinc; catalyticBy similarity | 1 | |
Sitei | 586 | Transition state stabilizerBy similarity | 1 |
GO - Molecular functioni
- metalloaminopeptidase activity Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- angiotensin maturation Source: Reactome
Keywordsi
Molecular function | Aminopeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8N6M6 |
Reactomei | R-HSA-2022377, Metabolism of Angiotensinogen to Angiotensins |
SABIO-RKi | Q8N6M6 |
Protein family/group databases
MEROPSi | M01.028 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000148120.14 |
HGNCi | HGNC:1361, AOPEP |
neXtProti | NX_Q8N6M6 |
Subcellular locationi
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 84909 |
OpenTargetsi | ENSG00000148120 |
PharmGKBi | PA25978 |
Miscellaneous databases
Pharosi | Q8N6M6, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3831223 |
Polymorphism and mutation databases
BioMutai | C9orf3 |
DMDMi | 61211648 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095091 | 1 – 819 | Aminopeptidase OAdd BLAST | 819 |
Proteomic databases
MassIVEi | Q8N6M6 |
PaxDbi | Q8N6M6 |
PeptideAtlasi | Q8N6M6 |
PRIDEi | Q8N6M6 |
ProteomicsDBi | 72195 [Q8N6M6-1] 72196 [Q8N6M6-2] 72197 [Q8N6M6-3] 72198 [Q8N6M6-4] |
PTM databases
iPTMneti | Q8N6M6 |
PhosphoSitePlusi | Q8N6M6 |
Expressioni
Tissue specificityi
Predominantly expressed in pancreas, placenta, liver, testis and heart. Expressed at lower level in brain, lung and kidney.1 Publication
Gene expression databases
Bgeei | ENSG00000148120, Expressed in thoracic aorta and 236 other tissues |
ExpressionAtlasi | Q8N6M6, baseline and differential |
Genevisiblei | Q8N6M6, HS |
Organism-specific databases
HPAi | ENSG00000148120, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 124346, 9 interactors |
IntActi | Q8N6M6, 16 interactors |
MINTi | Q8N6M6 |
STRINGi | 9606.ENSP00000364464 |
Miscellaneous databases
RNActi | Q8N6M6, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 689 – 699 | Nucleolar localization signalBy similarityAdd BLAST | 11 |
Sequence similaritiesi
Belongs to the peptidase M1 family.Curated
Phylogenomic databases
eggNOGi | KOG1047, Eukaryota |
GeneTreei | ENSGT00940000155211 |
InParanoidi | Q8N6M6 |
OMAi | PFMQVHY |
OrthoDBi | 775595at2759 |
PhylomeDBi | Q8N6M6 |
TreeFami | TF332004 |
Family and domain databases
CDDi | cd09599, M1_LTA4H, 1 hit |
Gene3Di | 1.25.40.320, 1 hit 2.60.40.1730, 1 hit |
InterProi | View protein in InterPro IPR042097, Aminopeptidase_N-like_N IPR033577, AOPep IPR016024, ARM-type_fold IPR038502, M1_LTA-4_hydro/amino_C_sf IPR034015, M1_LTA4H IPR015211, Peptidase_M1_C IPR014782, Peptidase_M1_dom |
PANTHERi | PTHR46627, PTHR46627, 1 hit |
Pfami | View protein in Pfam PF09127, Leuk-A4-hydro_C, 1 hit PF01433, Peptidase_M1, 1 hit |
SMARTi | View protein in SMART SM01263, Leuk-A4-hydro_C, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF63737, SSF63737, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8N6M6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDIQLDPARD DLPLMANTSH ILVKHYVLDL DVDFESQVIE GTIVLFLEDG
60 70 80 90 100
NRFKKQNSSI EEACQSESNK ACKFGMPEPC HIPVTNARTF SSEMEYNDFA
110 120 130 140 150
ICSKGEKDTS DKDGNHDNQE HASGISSSKY CCDTGNHGSE DFLLVLDCCD
160 170 180 190 200
LSVLKVEEVD VAAVPGLEKF TRSPELTVVS EEFRNQIVRE LVTLPANRWR
210 220 230 240 250
EQLDYYARCS QAPGCGELLF DTDTWSLQIR KTGAQTATDF PHAIRIWYKT
260 270 280 290 300
KPEGRSVTWT SDQSGRPCVY TVGSPINNRA LFPCQEPPVA MSTWQATVRA
310 320 330 340 350
AASFVVLMSG ENSAKPTQLW EECSSWYYYV TMPMPASTFT IAVGCWTEMK
360 370 380 390 400
METWSSNDLA TERPFSPSEA NFRHVGVCSH MEYPCRFQNA SATTQEIIPH
410 420 430 440 450
RVFAPVCLTG ACQETLLRLI PPCLSAAHSV LGAHPFSRLD VLIVPANFPS
460 470 480 490 500
LGMASPHIMF LSQSILTGGN HLCGTRLCHE IAHAWFGLAI GARDWTEEWL
510 520 530 540 550
SEGFATHLED VFWATAQQLA PYEAREQQEL RACLRWRRLQ DEMQCSPEEM
560 570 580 590 600
QVLRPSKDKT GHTSDSGASV IKHGLNPEKI FMQVHYLKGY FLLRFLAKRL
610 620 630 640 650
GDETYFSFLR KFVHTFHGQL ILSQDFLQML LENIPEEKRL ELSVENIYQD
660 670 680 690 700
WLESSGIPKP LQRERRAGAE CGLARQVRAE VTKWIGVNRR PRKRKRREKE
710 720 730 740 750
EVFEKLLPDQ LVLLLEHLLE QKTLSPRTLQ SLQRTYHLQD QDAEVRHRWC
760 770 780 790 800
ELIVKHKFTK AYKSVERFLQ EDQAMGVYLY GELMVSEDAR QQQLARRCFE
810
RTKEQMDRSS AQVVAEMLF
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MST7 | A0A0A0MST7_HUMAN | Aminopeptidase O | AOPEP | 601 | Annotation score: | ||
A0A0A0MSU0 | A0A0A0MSU0_HUMAN | Aminopeptidase O | AOPEP | 508 | Annotation score: | ||
H0Y7M4 | H0Y7M4_HUMAN | Aminopeptidase O | AOPEP | 184 | Annotation score: | ||
H0YCM2 | H0YCM2_HUMAN | Aminopeptidase O | AOPEP | 180 | Annotation score: | ||
H0Y7A8 | H0Y7A8_HUMAN | Aminopeptidase O | AOPEP | 145 | Annotation score: | ||
X6RBX4 | X6RBX4_HUMAN | Aminopeptidase O | AOPEP | 140 | Annotation score: |
Sequence cautioni
The sequence AAH20194 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB55210 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021511 | 179 | V → A. Corresponds to variant dbSNP:rs16911679Ensembl. | 1 | |
Natural variantiVAR_057053 | 179 | V → I. Corresponds to variant dbSNP:rs16911679Ensembl. | 1 | |
Natural variantiVAR_057054 | 255 | R → Q. Corresponds to variant dbSNP:rs16911681Ensembl. | 1 | |
Natural variantiVAR_057055 | 386 | R → C. Corresponds to variant dbSNP:rs34557833Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013161 | 455 – 553 | Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST | 99 | |
Alternative sequenceiVSP_013162 | 555 – 565 | PSKDKTGHTSD → FPHVGGCSGSFS in isoform 3. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_013163 | 566 – 819 | Missing in isoform 3. 1 PublicationAdd BLAST | 254 | |
Alternative sequenceiVSP_013164 | 774 – 784 | AMGVYLYGELM → VGVIFQQMGIL in isoform 4. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_013165 | 785 – 819 | Missing in isoform 4. 1 PublicationAdd BLAST | 35 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ560639 mRNA Translation: CAD90259.1 AK027581 mRNA Translation: BAB55210.1 Different initiation. AK057450 mRNA Translation: BAB71491.1 AL157936 Genomic DNA No translation available. AL353768 Genomic DNA No translation available. AL354893 Genomic DNA No translation available. BC020194 mRNA Translation: AAH20194.1 Different initiation. BC029616 mRNA Translation: AAH29616.1 |
CCDSi | CCDS55327.1 [Q8N6M6-3] CCDS55328.1 [Q8N6M6-1] CCDS6713.1 [Q8N6M6-2] |
RefSeqi | NP_001180258.1, NM_001193329.1 [Q8N6M6-1] NP_001180260.1, NM_001193331.2 [Q8N6M6-3] NP_116212.3, NM_032823.5 [Q8N6M6-2] XP_006717369.1, XM_006717306.1 [Q8N6M6-1] XP_016870719.1, XM_017015230.1 [Q8N6M6-1] XP_016870720.1, XM_017015231.1 [Q8N6M6-2] |
Genome annotation databases
Ensembli | ENST00000277198; ENSP00000277198; ENSG00000148120 [Q8N6M6-3] ENST00000297979; ENSP00000297979; ENSG00000148120 [Q8N6M6-2] ENST00000375315; ENSP00000364464; ENSG00000148120 [Q8N6M6-1] |
GeneIDi | 84909 |
KEGGi | hsa:84909 |
UCSCi | uc004aux.3, human [Q8N6M6-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ560639 mRNA Translation: CAD90259.1 AK027581 mRNA Translation: BAB55210.1 Different initiation. AK057450 mRNA Translation: BAB71491.1 AL157936 Genomic DNA No translation available. AL353768 Genomic DNA No translation available. AL354893 Genomic DNA No translation available. BC020194 mRNA Translation: AAH20194.1 Different initiation. BC029616 mRNA Translation: AAH29616.1 |
CCDSi | CCDS55327.1 [Q8N6M6-3] CCDS55328.1 [Q8N6M6-1] CCDS6713.1 [Q8N6M6-2] |
RefSeqi | NP_001180258.1, NM_001193329.1 [Q8N6M6-1] NP_001180260.1, NM_001193331.2 [Q8N6M6-3] NP_116212.3, NM_032823.5 [Q8N6M6-2] XP_006717369.1, XM_006717306.1 [Q8N6M6-1] XP_016870719.1, XM_017015230.1 [Q8N6M6-1] XP_016870720.1, XM_017015231.1 [Q8N6M6-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 124346, 9 interactors |
IntActi | Q8N6M6, 16 interactors |
MINTi | Q8N6M6 |
STRINGi | 9606.ENSP00000364464 |
Chemistry databases
ChEMBLi | CHEMBL3831223 |
Protein family/group databases
MEROPSi | M01.028 |
PTM databases
iPTMneti | Q8N6M6 |
PhosphoSitePlusi | Q8N6M6 |
Polymorphism and mutation databases
BioMutai | C9orf3 |
DMDMi | 61211648 |
Proteomic databases
MassIVEi | Q8N6M6 |
PaxDbi | Q8N6M6 |
PeptideAtlasi | Q8N6M6 |
PRIDEi | Q8N6M6 |
ProteomicsDBi | 72195 [Q8N6M6-1] 72196 [Q8N6M6-2] 72197 [Q8N6M6-3] 72198 [Q8N6M6-4] |
Protocols and materials databases
Antibodypediai | 985, 86 antibodies |
Genome annotation databases
Ensembli | ENST00000277198; ENSP00000277198; ENSG00000148120 [Q8N6M6-3] ENST00000297979; ENSP00000297979; ENSG00000148120 [Q8N6M6-2] ENST00000375315; ENSP00000364464; ENSG00000148120 [Q8N6M6-1] |
GeneIDi | 84909 |
KEGGi | hsa:84909 |
UCSCi | uc004aux.3, human [Q8N6M6-1] |
Organism-specific databases
CTDi | 84909 |
DisGeNETi | 84909 |
EuPathDBi | HostDB:ENSG00000148120.14 |
GeneCardsi | AOPEP |
HGNCi | HGNC:1361, AOPEP |
HPAi | ENSG00000148120, Low tissue specificity |
neXtProti | NX_Q8N6M6 |
OpenTargetsi | ENSG00000148120 |
PharmGKBi | PA25978 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1047, Eukaryota |
GeneTreei | ENSGT00940000155211 |
InParanoidi | Q8N6M6 |
OMAi | PFMQVHY |
OrthoDBi | 775595at2759 |
PhylomeDBi | Q8N6M6 |
TreeFami | TF332004 |
Enzyme and pathway databases
PathwayCommonsi | Q8N6M6 |
Reactomei | R-HSA-2022377, Metabolism of Angiotensinogen to Angiotensins |
SABIO-RKi | Q8N6M6 |
Miscellaneous databases
BioGRID-ORCSi | 84909, 2 hits in 843 CRISPR screens |
ChiTaRSi | C9orf3, human |
GenomeRNAii | 84909 |
Pharosi | Q8N6M6, Tbio |
PROi | PR:Q8N6M6 |
RNActi | Q8N6M6, protein |
Gene expression databases
Bgeei | ENSG00000148120, Expressed in thoracic aorta and 236 other tissues |
ExpressionAtlasi | Q8N6M6, baseline and differential |
Genevisiblei | Q8N6M6, HS |
Family and domain databases
CDDi | cd09599, M1_LTA4H, 1 hit |
Gene3Di | 1.25.40.320, 1 hit 2.60.40.1730, 1 hit |
InterProi | View protein in InterPro IPR042097, Aminopeptidase_N-like_N IPR033577, AOPep IPR016024, ARM-type_fold IPR038502, M1_LTA-4_hydro/amino_C_sf IPR034015, M1_LTA4H IPR015211, Peptidase_M1_C IPR014782, Peptidase_M1_dom |
PANTHERi | PTHR46627, PTHR46627, 1 hit |
Pfami | View protein in Pfam PF09127, Leuk-A4-hydro_C, 1 hit PF01433, Peptidase_M1, 1 hit |
SMARTi | View protein in SMART SM01263, Leuk-A4-hydro_C, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF63737, SSF63737, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMPO_HUMAN | |
Accessioni | Q8N6M6Primary (citable) accession number: Q8N6M6 Secondary accession number(s): Q5T9B1 Q96SS1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | March 15, 2005 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Peptidase families
Classification of peptidase families and list of entries