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Protein

ADP-ribosylation factor GTPase-activating protein 2

Gene

ARFGAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.1 Publication

Miscellaneous

Vero cells overexpressing truncated ARFGAP2 show accumulation of cholera toxin A subunit in the Golgi complex rather than the endoplasmic reticulum.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri26 – 49C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
Biological processER-Golgi transport, Protein transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor GTPase-activating protein 2
Short name:
ARF GAP 2
Alternative name(s):
GTPase-activating protein ZNF289
Zinc finger protein 289
Gene namesi
Name:ARFGAP2
Synonyms:ZNF289
ORF Names:Nbla10535
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000149182.14
HGNCiHGNC:13504 ARFGAP2
MIMi606908 gene
neXtProtiNX_Q8N6H7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi84364
OpenTargetsiENSG00000149182
PharmGKBiPA162376868

Polymorphism and mutation databases

BioMutaiARFGAP2
DMDMi74729129

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002784682 – 521ADP-ribosylation factor GTPase-activating protein 2Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei140PhosphoserineCombined sources1
Modified residuei146PhosphoserineCombined sources1
Modified residuei201PhosphoserineCombined sources1
Modified residuei237PhosphoserineCombined sources1
Modified residuei240PhosphoserineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei334PhosphoserineCombined sources1
Modified residuei340PhosphoserineCombined sources1
Modified residuei364PhosphoserineCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei432PhosphoserineCombined sources1
Modified residuei433PhosphoserineCombined sources1
Modified residuei513PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8N6H7
MaxQBiQ8N6H7
PaxDbiQ8N6H7
PeptideAtlasiQ8N6H7
PRIDEiQ8N6H7
ProteomicsDBi72177

PTM databases

iPTMnetiQ8N6H7
PhosphoSitePlusiQ8N6H7

Expressioni

Gene expression databases

BgeeiENSG00000149182 Expressed in 224 organ(s), highest expression level in skin of leg
CleanExiHS_ARFGAP2
ExpressionAtlasiQ8N6H7 baseline and differential
GenevisibleiQ8N6H7 HS

Organism-specific databases

HPAiHPA016649
HPA018152

Interactioni

Subunit structurei

Interacts with the coatomer complex. Interacts with the C-terminal appendage domain of COPG1.2 Publications

Protein-protein interaction databases

BioGridi124065, 20 interactors
IntActiQ8N6H7, 7 interactors
STRINGi9606.ENSP00000434442

Structurei

Secondary structure

1521
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8N6H7
SMRiQ8N6H7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N6H7

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 127Arf-GAPPROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 521Required for interaction with coatomerAdd BLAST425

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili242 – 308Sequence analysisAdd BLAST67

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri26 – 49C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0706 Eukaryota
COG5347 LUCA
GeneTreeiENSGT00390000005436
HOGENOMiHOG000162970
HOVERGENiHBG050563
InParanoidiQ8N6H7
KOiK12493
OMAiDQWQWDQ
OrthoDBiEOG091G0AS9
PhylomeDBiQ8N6H7
TreeFamiTF313985

Family and domain databases

Gene3Di3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
PfamiView protein in Pfam
PF01412 ArfGap, 1 hit
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00105 ArfGap, 1 hit
SUPFAMiSSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50115 ARFGAP, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 17 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q8N6H7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAEPNKTEI QTLFKRLRAV PTNKACFDCG AKNPSWASIT YGVFLCIDCS
60 70 80 90 100
GVHRSLGVHL SFIRSTELDS NWNWFQLRCM QVGGNANATA FFRQHGCTAN
110 120 130 140 150
DANTKYNSRA AQMYREKIRQ LGSAALARHG TDLWIDNMSS AVPNHSPEKK
160 170 180 190 200
DSDFFTEHTQ PPAWDAPATE PSGTQQPAPS TESSGLAQPE HGPNTDLLGT
210 220 230 240 250
SPKASLELKS SIIGKKKPAA AKKGLGAKKG LGAQKVSSQS FSEIERQAQV
260 270 280 290 300
AEKLREQQAA DAKKQAEESM VASMRLAYQE LQIDRKKEEK KLQNLEGKKR
310 320 330 340 350
EQAERLGMGL VSRSSVSHSV LSEMQVIEQE TPVSAKSSRS QLDLFDDVGT
360 370 380 390 400
FASGPPKYKD NPFSLGESFG SRWDTDAAWG MDRVEEKEPE VTISSIRPIS
410 420 430 440 450
ERATNRREVE SRSSGLESSE ARQKFAGAKA ISSDMFFGRE VDAEYEARSR
460 470 480 490 500
LQQLSGSSAI SSSDLFGDMD GAHGAGSVSL GNVLPTADIA QFKQGVKSVA
510 520
GKMAVLANGV MNSLQDRYGS Y
Length:521
Mass (Da):56,720
Last modified:October 1, 2002 - v1
Checksum:i16655EBA94D29E81
GO
Isoform 2 (identifier: Q8N6H7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.
     133-160: Missing.

Note: No experimental confirmation available.
Show »
Length:414
Mass (Da):44,543
Checksum:iCB86DEEF636E8D91
GO
Isoform 3 (identifier: Q8N6H7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-269: Missing.

Note: No experimental confirmation available.
Show »
Length:252
Mass (Da):27,535
Checksum:i8DFD0EE52D062600
GO

Computationally mapped potential isoform sequencesi

There are 17 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PIY6E9PIY6_HUMAN
ADP-ribosylation factor GTPase-acti...
ARFGAP2
327Annotation score:
E9PN48E9PN48_HUMAN
ADP-ribosylation factor GTPase-acti...
ARFGAP2
252Annotation score:
A0A0D9SF70A0A0D9SF70_HUMAN
ADP-ribosylation factor GTPase-acti...
ARFGAP2 ZNF289, hCG_1811553
385Annotation score:
G5E9L0G5E9L0_HUMAN
ADP-ribosylation factor GTPase-acti...
ARFGAP2
493Annotation score:
E9PQP3E9PQP3_HUMAN
ADP-ribosylation factor GTPase-acti...
ARFGAP2
274Annotation score:
E9PNY8E9PNY8_HUMAN
ADP-ribosylation factor GTPase-acti...
ARFGAP2
110Annotation score:
E9PM18E9PM18_HUMAN
ADP-ribosylation factor GTPase-acti...
ARFGAP2
70Annotation score:
E9PM63E9PM63_HUMAN
ADP-ribosylation factor GTPase-acti...
ARFGAP2
94Annotation score:
E9PMR9E9PMR9_HUMAN
ADP-ribosylation factor GTPase-acti...
ARFGAP2
89Annotation score:
H0YDN9H0YDN9_HUMAN
ADP-ribosylation factor GTPase-acti...
ARFGAP2
237Annotation score:
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40T → M in BAB55144 (PubMed:14702039).Curated1
Sequence conflicti107N → S in BAB55144 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048321143P → R. Corresponds to variant dbSNP:rs11542793Ensembl.1
Natural variantiVAR_048322339R → H. Corresponds to variant dbSNP:rs34662994Ensembl.1
Natural variantiVAR_048323406R → W. Corresponds to variant dbSNP:rs35950498Ensembl.1
Natural variantiVAR_030780411S → N1 PublicationCorresponds to variant dbSNP:rs3740691Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0560541 – 269Missing in isoform 3. 1 PublicationAdd BLAST269
Alternative sequenceiVSP_0560551 – 79Missing in isoform 2. 1 PublicationAdd BLAST79
Alternative sequenceiVSP_056056133 – 160Missing in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027482 mRNA Translation: BAB55144.1
AK301785 mRNA Translation: BAG63241.1
AK315992 mRNA Translation: BAH14363.1
AC090589 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW67963.1
CH471064 Genomic DNA Translation: EAW67968.1
BC030148 mRNA Translation: AAH30148.1
AL834337 mRNA Translation: CAD39004.1
AB073358 mRNA Translation: BAE45716.1
CCDSiCCDS7926.1 [Q8N6H7-1]
RefSeqiNP_001229761.1, NM_001242832.1
NP_115765.2, NM_032389.4 [Q8N6H7-1]
UniGeneiHs.436204

Genome annotation databases

EnsembliENST00000524782; ENSP00000434442; ENSG00000149182 [Q8N6H7-1]
GeneIDi84364
KEGGihsa:84364
UCSCiuc001ndt.4 human [Q8N6H7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiARFG2_HUMAN
AccessioniPrimary (citable) accession number: Q8N6H7
Secondary accession number(s): B4DX29
, B7Z9M7, D3DQQ9, Q3LIF2, Q8N3I1, Q96SX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 1, 2002
Last modified: September 12, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health

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