UniProtKB - Q8N6H7 (ARFG2_HUMAN)
Protein
ADP-ribosylation factor GTPase-activating protein 2
Gene
ARFGAP2
Organism
Homo sapiens (Human)
Status
Functioni
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.1 Publication
Miscellaneous
Vero cells overexpressing truncated ARFGAP2 show accumulation of cholera toxin A subunit in the Golgi complex rather than the endoplasmic reticulum.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 26 – 49 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- GTPase activator activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- COPI coating of Golgi vesicle Source: GO_Central
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
- protein transport Source: UniProtKB-KW
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: Reactome
Keywordsi
Molecular function | GTPase activation |
Biological process | ER-Golgi transport, Protein transport, Transport |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8N6H7 |
Reactomei | R-HSA-6807878, COPI-mediated anterograde transport R-HSA-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Names & Taxonomyi
Protein namesi | Recommended name: ADP-ribosylation factor GTPase-activating protein 2Short name: ARF GAP 2 Alternative name(s): GTPase-activating protein ZNF289 Zinc finger protein 289 |
Gene namesi | Name:ARFGAP2 Synonyms:ZNF289 ORF Names:Nbla10535 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:13504, ARFGAP2 |
MIMi | 606908, gene |
neXtProti | NX_Q8N6H7 |
VEuPathDBi | HostDB:ENSG00000149182.14 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Note: Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
Cytosol
- cytosol Source: HPA
Golgi apparatus
- Golgi apparatus Source: HPA
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: HPA
Keywords - Cellular componenti
Cytoplasm, Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 84364 |
OpenTargetsi | ENSG00000149182 |
PharmGKBi | PA162376868 |
Miscellaneous databases
Pharosi | Q8N6H7, Tbio |
Genetic variation databases
BioMutai | ARFGAP2 |
DMDMi | 74729129 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000278468 | 2 – 521 | ADP-ribosylation factor GTPase-activating protein 2Add BLAST | 520 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 140 | PhosphoserineCombined sources | 1 | |
Modified residuei | 146 | PhosphoserineCombined sources | 1 | |
Modified residuei | 201 | PhosphoserineCombined sources | 1 | |
Modified residuei | 237 | PhosphoserineCombined sources | 1 | |
Modified residuei | 240 | PhosphoserineCombined sources | 1 | |
Modified residuei | 312 | PhosphoserineCombined sources | 1 | |
Modified residuei | 334 | PhosphoserineCombined sources | 1 | |
Modified residuei | 340 | PhosphoserineCombined sources | 1 | |
Modified residuei | 364 | PhosphoserineCombined sources | 1 | |
Modified residuei | 368 | PhosphoserineCombined sources | 1 | |
Modified residuei | 432 | PhosphoserineCombined sources | 1 | |
Modified residuei | 433 | PhosphoserineCombined sources | 1 | |
Modified residuei | 513 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8N6H7 |
jPOSTi | Q8N6H7 |
MassIVEi | Q8N6H7 |
MaxQBi | Q8N6H7 |
PaxDbi | Q8N6H7 |
PeptideAtlasi | Q8N6H7 |
PRIDEi | Q8N6H7 |
ProteomicsDBi | 5401 72177 [Q8N6H7-1] |
PTM databases
iPTMneti | Q8N6H7 |
MetOSitei | Q8N6H7 |
PhosphoSitePlusi | Q8N6H7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000149182, Expressed in stomach and 238 other tissues |
ExpressionAtlasi | Q8N6H7, baseline and differential |
Genevisiblei | Q8N6H7, HS |
Organism-specific databases
HPAi | ENSG00000149182, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with the coatomer complex.
Interacts with the C-terminal appendage domain of COPG1.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 124065, 75 interactors |
IntActi | Q8N6H7, 14 interactors |
MINTi | Q8N6H7 |
STRINGi | 9606.ENSP00000434442 |
Miscellaneous databases
RNActi | Q8N6H7, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8N6H7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8N6H7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 127 | Arf-GAPPROSITE-ProRule annotationAdd BLAST | 117 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 97 – 521 | Required for interaction with coatomerAdd BLAST | 425 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 242 – 308 | Sequence analysisAdd BLAST | 67 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 26 – 49 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0706, Eukaryota |
GeneTreei | ENSGT00940000155568 |
InParanoidi | Q8N6H7 |
OMAi | FDCQARN |
OrthoDBi | 1155557at2759 |
PhylomeDBi | Q8N6H7 |
TreeFami | TF313985 |
Family and domain databases
Gene3Di | 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf |
Pfami | View protein in Pfam PF01412, ArfGap, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00105, ArfGap, 1 hit |
SUPFAMi | SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115, ARFGAP, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 17 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8N6H7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAEPNKTEI QTLFKRLRAV PTNKACFDCG AKNPSWASIT YGVFLCIDCS
60 70 80 90 100
GVHRSLGVHL SFIRSTELDS NWNWFQLRCM QVGGNANATA FFRQHGCTAN
110 120 130 140 150
DANTKYNSRA AQMYREKIRQ LGSAALARHG TDLWIDNMSS AVPNHSPEKK
160 170 180 190 200
DSDFFTEHTQ PPAWDAPATE PSGTQQPAPS TESSGLAQPE HGPNTDLLGT
210 220 230 240 250
SPKASLELKS SIIGKKKPAA AKKGLGAKKG LGAQKVSSQS FSEIERQAQV
260 270 280 290 300
AEKLREQQAA DAKKQAEESM VASMRLAYQE LQIDRKKEEK KLQNLEGKKR
310 320 330 340 350
EQAERLGMGL VSRSSVSHSV LSEMQVIEQE TPVSAKSSRS QLDLFDDVGT
360 370 380 390 400
FASGPPKYKD NPFSLGESFG SRWDTDAAWG MDRVEEKEPE VTISSIRPIS
410 420 430 440 450
ERATNRREVE SRSSGLESSE ARQKFAGAKA ISSDMFFGRE VDAEYEARSR
460 470 480 490 500
LQQLSGSSAI SSSDLFGDMD GAHGAGSVSL GNVLPTADIA QFKQGVKSVA
510 520
GKMAVLANGV MNSLQDRYGS Y
Computationally mapped potential isoform sequencesi
There are 17 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PN48 | E9PN48_HUMAN | ADP-ribosylation factor GTPase-acti... | ARFGAP2 | 252 | Annotation score: | ||
E9PIY6 | E9PIY6_HUMAN | ADP-ribosylation factor GTPase-acti... | ARFGAP2 | 327 | Annotation score: | ||
A0A0D9SF70 | A0A0D9SF70_HUMAN | ADP-ribosylation factor GTPase-acti... | ARFGAP2 ZNF289, hCG_1811553 | 385 | Annotation score: | ||
G5E9L0 | G5E9L0_HUMAN | ADP-ribosylation factor GTPase-acti... | ARFGAP2 | 493 | Annotation score: | ||
E9PMU9 | E9PMU9_HUMAN | ADP-ribosylation factor GTPase-acti... | ARFGAP2 | 156 | Annotation score: | ||
E9PQP3 | E9PQP3_HUMAN | ADP-ribosylation factor GTPase-acti... | ARFGAP2 | 274 | Annotation score: | ||
E9PK28 | E9PK28_HUMAN | ADP-ribosylation factor GTPase-acti... | ARFGAP2 | 238 | Annotation score: | ||
H0YF45 | H0YF45_HUMAN | ADP-ribosylation factor GTPase-acti... | ARFGAP2 | 119 | Annotation score: | ||
H0YDN9 | H0YDN9_HUMAN | ADP-ribosylation factor GTPase-acti... | ARFGAP2 | 237 | Annotation score: | ||
E9PJT7 | E9PJT7_HUMAN | ADP-ribosylation factor GTPase-acti... | ARFGAP2 | 173 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 40 | T → M in BAB55144 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 107 | N → S in BAB55144 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048321 | 143 | P → R. Corresponds to variant dbSNP:rs11542793Ensembl. | 1 | |
Natural variantiVAR_048322 | 339 | R → H. Corresponds to variant dbSNP:rs34662994Ensembl. | 1 | |
Natural variantiVAR_048323 | 406 | R → W. Corresponds to variant dbSNP:rs35950498Ensembl. | 1 | |
Natural variantiVAR_030780 | 411 | S → N1 PublicationCorresponds to variant dbSNP:rs3740691Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056054 | 1 – 269 | Missing in isoform 3. 1 PublicationAdd BLAST | 269 | |
Alternative sequenceiVSP_056055 | 1 – 79 | Missing in isoform 2. 1 PublicationAdd BLAST | 79 | |
Alternative sequenceiVSP_056056 | 133 – 160 | Missing in isoform 2. 1 PublicationAdd BLAST | 28 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK027482 mRNA Translation: BAB55144.1 AK301785 mRNA Translation: BAG63241.1 AK315992 mRNA Translation: BAH14363.1 AC090589 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW67963.1 CH471064 Genomic DNA Translation: EAW67968.1 BC030148 mRNA Translation: AAH30148.1 AL834337 mRNA Translation: CAD39004.1 AB073358 mRNA Translation: BAE45716.1 |
CCDSi | CCDS7926.1 [Q8N6H7-1] |
RefSeqi | NP_001229761.1, NM_001242832.1 NP_115765.2, NM_032389.4 [Q8N6H7-1] |
Genome annotation databases
Ensembli | ENST00000524782; ENSP00000434442; ENSG00000149182 [Q8N6H7-1] |
GeneIDi | 84364 |
KEGGi | hsa:84364 |
UCSCi | uc001ndt.4, human [Q8N6H7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK027482 mRNA Translation: BAB55144.1 AK301785 mRNA Translation: BAG63241.1 AK315992 mRNA Translation: BAH14363.1 AC090589 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW67963.1 CH471064 Genomic DNA Translation: EAW67968.1 BC030148 mRNA Translation: AAH30148.1 AL834337 mRNA Translation: CAD39004.1 AB073358 mRNA Translation: BAE45716.1 |
CCDSi | CCDS7926.1 [Q8N6H7-1] |
RefSeqi | NP_001229761.1, NM_001242832.1 NP_115765.2, NM_032389.4 [Q8N6H7-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2P57 | X-ray | 1.80 | A | 3-130 | [»] | |
SMRi | Q8N6H7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124065, 75 interactors |
IntActi | Q8N6H7, 14 interactors |
MINTi | Q8N6H7 |
STRINGi | 9606.ENSP00000434442 |
PTM databases
iPTMneti | Q8N6H7 |
MetOSitei | Q8N6H7 |
PhosphoSitePlusi | Q8N6H7 |
Genetic variation databases
BioMutai | ARFGAP2 |
DMDMi | 74729129 |
Proteomic databases
EPDi | Q8N6H7 |
jPOSTi | Q8N6H7 |
MassIVEi | Q8N6H7 |
MaxQBi | Q8N6H7 |
PaxDbi | Q8N6H7 |
PeptideAtlasi | Q8N6H7 |
PRIDEi | Q8N6H7 |
ProteomicsDBi | 5401 72177 [Q8N6H7-1] |
Protocols and materials databases
Antibodypediai | 13559, 177 antibodies |
DNASUi | 84364 |
Genome annotation databases
Ensembli | ENST00000524782; ENSP00000434442; ENSG00000149182 [Q8N6H7-1] |
GeneIDi | 84364 |
KEGGi | hsa:84364 |
UCSCi | uc001ndt.4, human [Q8N6H7-1] |
Organism-specific databases
CTDi | 84364 |
DisGeNETi | 84364 |
GeneCardsi | ARFGAP2 |
HGNCi | HGNC:13504, ARFGAP2 |
HPAi | ENSG00000149182, Low tissue specificity |
MIMi | 606908, gene |
neXtProti | NX_Q8N6H7 |
OpenTargetsi | ENSG00000149182 |
PharmGKBi | PA162376868 |
VEuPathDBi | HostDB:ENSG00000149182.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0706, Eukaryota |
GeneTreei | ENSGT00940000155568 |
InParanoidi | Q8N6H7 |
OMAi | FDCQARN |
OrthoDBi | 1155557at2759 |
PhylomeDBi | Q8N6H7 |
TreeFami | TF313985 |
Enzyme and pathway databases
PathwayCommonsi | Q8N6H7 |
Reactomei | R-HSA-6807878, COPI-mediated anterograde transport R-HSA-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Miscellaneous databases
BioGRID-ORCSi | 84364, 11 hits in 1000 CRISPR screens |
ChiTaRSi | ARFGAP2, human |
EvolutionaryTracei | Q8N6H7 |
GenomeRNAii | 84364 |
Pharosi | Q8N6H7, Tbio |
PROi | PR:Q8N6H7 |
RNActi | Q8N6H7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000149182, Expressed in stomach and 238 other tissues |
ExpressionAtlasi | Q8N6H7, baseline and differential |
Genevisiblei | Q8N6H7, HS |
Family and domain databases
Gene3Di | 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf |
Pfami | View protein in Pfam PF01412, ArfGap, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00105, ArfGap, 1 hit |
SUPFAMi | SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115, ARFGAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARFG2_HUMAN | |
Accessioni | Q8N6H7Primary (citable) accession number: Q8N6H7 Secondary accession number(s): B4DX29 Q96SX7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | October 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references