UniProtKB - Q8N6G5 (CGAT2_HUMAN)
Protein
Chondroitin sulfate N-acetylgalactosaminyltransferase 2
Gene
CSGALNACT2
Organism
Homo sapiens (Human)
Status
Functioni
Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains.2 Publications
Catalytic activityi
- 3-O-(β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl)-L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-(β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl)-L-seryl-[protein] + H+ + UDPEC:2.4.1.174
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 369 | Divalent metal cationSequence analysis | 1 | |
Metal bindingi | 486 | Divalent metal cationSequence analysis | 1 |
GO - Molecular functioni
- acetylgalactosaminyltransferase activity Source: UniProtKB
- glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity Source: GO_Central
- glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- chondroitin sulfate biosynthetic process Source: GO_Central
- chondroitin sulfate proteoglycan biosynthetic process Source: UniProtKB
- chondroitin sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: UniProtKB
- dermatan sulfate proteoglycan biosynthetic process Source: UniProtKB
- dermatan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: UniProtKB
- proteoglycan biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Ligand | Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:HS10013-MONOMER |
BRENDAi | 2.4.1.174, 2681 2.4.1.175, 2681 |
PathwayCommonsi | Q8N6G5 |
Reactomei | R-HSA-2022870, Chondroitin sulfate biosynthesis |
Protein family/group databases
CAZyi | GT7, Glycosyltransferase Family 7 |
Names & Taxonomyi
Protein namesi | Recommended name: Chondroitin sulfate N-acetylgalactosaminyltransferase 2 (EC:2.4.1.174)Alternative name(s): Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2 Short name: Beta4GalNAcT-2 Short name: GalNAcT-2 |
Gene namesi | Name:CSGALNACT2 Synonyms:CHGN2, GALNACT2 ORF Names:PRO0082 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24292, CSGALNACT2 |
MIMi | 616616, gene |
neXtProti | NX_Q8N6G5 |
VEuPathDBi | HostDB:ENSG00000169826.7 |
Subcellular locationi
Golgi apparatus
- Golgi stack membrane Curated; Single-pass type II membrane protein Curated
Golgi apparatus
- Golgi cisterna membrane Source: UniProtKB-SubCell
- Golgi membrane Source: Reactome
- integral component of Golgi membrane Source: UniProtKB
Other locations
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 32 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 33 – 542 | LumenalSequence analysisAdd BLAST | 510 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 55454 |
OpenTargetsi | ENSG00000169826 |
PharmGKBi | PA162382854 |
Miscellaneous databases
Pharosi | Q8N6G5, Tbio |
Genetic variation databases
BioMutai | CSGALNACT2 |
DMDMi | 60391915 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000189566 | 1 – 542 | Chondroitin sulfate N-acetylgalactosaminyltransferase 2Add BLAST | 542 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 41 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 333 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | Q8N6G5 |
jPOSTi | Q8N6G5 |
MassIVEi | Q8N6G5 |
MaxQBi | Q8N6G5 |
PaxDbi | Q8N6G5 |
PeptideAtlasi | Q8N6G5 |
PRIDEi | Q8N6G5 |
ProteomicsDBi | 72168 [Q8N6G5-1] 72169 [Q8N6G5-2] |
PTM databases
GlyGeni | Q8N6G5, 2 sites |
iPTMneti | Q8N6G5 |
PhosphoSitePlusi | Q8N6G5 |
Expressioni
Tissue specificityi
Ubiquitous.2 Publications
Gene expression databases
Bgeei | ENSG00000169826, Expressed in bone marrow and 238 other tissues |
ExpressionAtlasi | Q8N6G5, baseline and differential |
Genevisiblei | Q8N6G5, HS |
Organism-specific databases
HPAi | ENSG00000169826, Tissue enhanced (bone) |
Interactioni
Binary interactionsi
Hide detailsQ8N6G5
Protein-protein interaction databases
BioGRIDi | 120672, 91 interactors |
IntActi | Q8N6G5, 78 interactors |
STRINGi | 9606.ENSP00000363590 |
Miscellaneous databases
RNActi | Q8N6G5, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 59 – 105 | Sequence analysisAdd BLAST | 47 |
Sequence similaritiesi
Belongs to the chondroitin N-acetylgalactosaminyltransferase family.Curated
Keywords - Domaini
Coiled coil, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3588, Eukaryota |
GeneTreei | ENSGT01010000222280 |
HOGENOMi | CLU_025958_0_1_1 |
InParanoidi | Q8N6G5 |
OMAi | GMMVFRE |
PhylomeDBi | Q8N6G5 |
TreeFami | TF318303 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR008428, Chond_GalNAc IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF05679, CHGN, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8N6G5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPRRGLILHT RTHWLLLGLA LLCSLVLFMY LLECAPQTDG NASLPGVVGE
60 70 80 90 100
NYGKEYYQAL LQEQEEHYQT RATSLKRQIA QLKQELQEMS EKMRSLQERR
110 120 130 140 150
NVGANGIGYQ SNKEQAPSDL LEFLHSQIDK AEVSIGAKLP SEYGVIPFES
160 170 180 190 200
FTLMKVFQLE MGLTRHPEEK PVRKDKRDEL VEVIEAGLEV INNPDEDDEQ
210 220 230 240 250
EDEEGPLGEK LIFNENDFVE GYYRTERDKG TQYELFFKKA DLTEYRHVTL
260 270 280 290 300
FRPFGPLMKV KSEMIDITRS IINIIVPLAE RTEAFVQFMQ NFRDVCIHQD
310 320 330 340 350
KKIHLTVVYF GKEGLSKVKS ILESVTSESN FHNYTLVSLN EEFNRGRGLN
360 370 380 390 400
VGARAWDKGE VLMFFCDVDI YFSAEFLNSC RLNAEPGKKV FYPVVFSLYN
410 420 430 440 450
PAIVYANQEV PPPVEQQLVH KKDSGFWRDF GFGMTCQYRS DFLTIGGFDM
460 470 480 490 500
EVKGWGGEDV HLYRKYLHGD LIVIRTPVPG LFHLWHEKRC ADELTPEQYR
510 520 530 540
MCIQSKAMNE ASHSHLGMLV FREEIETHLH KQAYRTNSEA VG
Sequence cautioni
The sequence AAF71068 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 438 | Y → H in BAB85092 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048715 | 215 | E → K. Corresponds to variant dbSNP:rs11238456Ensembl. | 1 | |
Natural variantiVAR_048716 | 479 | P → S1 PublicationCorresponds to variant dbSNP:rs2435381Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012729 | 327 – 333 | SESNFHN → RLASSTW in isoform 2. 1 Publication | 7 | |
Alternative sequenceiVSP_012730 | 334 – 542 | Missing in isoform 2. 1 PublicationAdd BLAST | 209 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB079252 mRNA Translation: BAC55935.1 AB090811 mRNA Translation: BAC55936.1 AK074474 mRNA Translation: BAB85092.1 AK125300 mRNA Translation: BAG54179.1 BX640967 mRNA Translation: CAE45982.1 BX641073 mRNA Translation: CAE46036.1 CH471160 Genomic DNA Translation: EAW86587.1 BC030268 mRNA Translation: AAH30268.1 AF116646 mRNA Translation: AAF71068.1 Different initiation. |
CCDSi | CCDS7201.1 [Q8N6G5-1] |
RefSeqi | NP_001306583.1, NM_001319654.1 NP_001306585.1, NM_001319656.1 NP_061060.3, NM_018590.4 [Q8N6G5-1] |
Genome annotation databases
Ensembli | ENST00000374466; ENSP00000363590; ENSG00000169826 [Q8N6G5-1] |
GeneIDi | 55454 |
KEGGi | hsa:55454 |
UCSCi | uc001jan.5, human [Q8N6G5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB079252 mRNA Translation: BAC55935.1 AB090811 mRNA Translation: BAC55936.1 AK074474 mRNA Translation: BAB85092.1 AK125300 mRNA Translation: BAG54179.1 BX640967 mRNA Translation: CAE45982.1 BX641073 mRNA Translation: CAE46036.1 CH471160 Genomic DNA Translation: EAW86587.1 BC030268 mRNA Translation: AAH30268.1 AF116646 mRNA Translation: AAF71068.1 Different initiation. |
CCDSi | CCDS7201.1 [Q8N6G5-1] |
RefSeqi | NP_001306583.1, NM_001319654.1 NP_001306585.1, NM_001319656.1 NP_061060.3, NM_018590.4 [Q8N6G5-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 120672, 91 interactors |
IntActi | Q8N6G5, 78 interactors |
STRINGi | 9606.ENSP00000363590 |
Protein family/group databases
CAZyi | GT7, Glycosyltransferase Family 7 |
PTM databases
GlyGeni | Q8N6G5, 2 sites |
iPTMneti | Q8N6G5 |
PhosphoSitePlusi | Q8N6G5 |
Genetic variation databases
BioMutai | CSGALNACT2 |
DMDMi | 60391915 |
Proteomic databases
EPDi | Q8N6G5 |
jPOSTi | Q8N6G5 |
MassIVEi | Q8N6G5 |
MaxQBi | Q8N6G5 |
PaxDbi | Q8N6G5 |
PeptideAtlasi | Q8N6G5 |
PRIDEi | Q8N6G5 |
ProteomicsDBi | 72168 [Q8N6G5-1] 72169 [Q8N6G5-2] |
Protocols and materials databases
Antibodypediai | 26871, 199 antibodies |
DNASUi | 55454 |
Genome annotation databases
Ensembli | ENST00000374466; ENSP00000363590; ENSG00000169826 [Q8N6G5-1] |
GeneIDi | 55454 |
KEGGi | hsa:55454 |
UCSCi | uc001jan.5, human [Q8N6G5-1] |
Organism-specific databases
CTDi | 55454 |
DisGeNETi | 55454 |
GeneCardsi | CSGALNACT2 |
HGNCi | HGNC:24292, CSGALNACT2 |
HPAi | ENSG00000169826, Tissue enhanced (bone) |
MIMi | 616616, gene |
neXtProti | NX_Q8N6G5 |
OpenTargetsi | ENSG00000169826 |
PharmGKBi | PA162382854 |
VEuPathDBi | HostDB:ENSG00000169826.7 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3588, Eukaryota |
GeneTreei | ENSGT01010000222280 |
HOGENOMi | CLU_025958_0_1_1 |
InParanoidi | Q8N6G5 |
OMAi | GMMVFRE |
PhylomeDBi | Q8N6G5 |
TreeFami | TF318303 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS10013-MONOMER |
BRENDAi | 2.4.1.174, 2681 2.4.1.175, 2681 |
PathwayCommonsi | Q8N6G5 |
Reactomei | R-HSA-2022870, Chondroitin sulfate biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 55454, 5 hits in 878 CRISPR screens |
ChiTaRSi | CSGALNACT2, human |
GenomeRNAii | 55454 |
Pharosi | Q8N6G5, Tbio |
PROi | PR:Q8N6G5 |
RNActi | Q8N6G5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000169826, Expressed in bone marrow and 238 other tissues |
ExpressionAtlasi | Q8N6G5, baseline and differential |
Genevisiblei | Q8N6G5, HS |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR008428, Chond_GalNAc IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF05679, CHGN, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CGAT2_HUMAN | |
Accessioni | Q8N6G5Primary (citable) accession number: Q8N6G5 Secondary accession number(s): B3KWL7 Q9P1I6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human entries with genetic variants
List of human entries with genetic variants