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Protein

Germinal center-associated signaling and motility protein

Gene

GCSAM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.3 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • myosin II binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

  • negative regulation of lymphocyte migration Source: UniProtKB
  • regulation of B cell receptor signaling pathway Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Germinal center-associated signaling and motility protein
Alternative name(s):
Germinal center B-cell-expressed transcript 2 protein
Germinal center-associated lymphoma protein
Short name:
hGAL
Gene namesi
Name:GCSAM
Synonyms:GAL, GCET2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000174500.12
HGNCiHGNC:20253 GCSAM
MIMi607792 gene
neXtProtiNX_Q8N6F7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi106 – 107YY → AA: Does not affect the interaction with SYK. 1 Publication2
Mutagenesisi128Y → F: Does not affect IL6 induced phosphorylation. Does not affect the interaction with SYK. 2 Publications1
Mutagenesisi148Y → F: Prevents IL6 induced phosphorylation. Does not affect the interaction with SYK. 2 Publications1

Organism-specific databases

DisGeNETi257144
OpenTargetsiENSG00000174500
PharmGKBiPA134980592

Polymorphism and mutation databases

BioMutaiGCSAM

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002562281 – 178Germinal center-associated signaling and motility proteinAdd BLAST178

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei99PhosphoserineBy similarity1
Modified residuei148PhosphotyrosineCombined sources1

Post-translational modificationi

Phosphorylation on tyrosine residues can be induced by IL6. Phosphorylation is mediated by LYN.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8N6F7
PaxDbiQ8N6F7
PeptideAtlasiQ8N6F7
PRIDEiQ8N6F7
ProteomicsDBi72164

PTM databases

iPTMnetiQ8N6F7
PhosphoSitePlusiQ8N6F7
SwissPalmiQ8N6F7

Expressioni

Tissue specificityi

Expressed in diffuse large B-cell lymphoma (DLBCL) and several germinal center (GC)-like lymphoma cell lines (at protein level). Highly expressed in normal GC lymphocytes and GC-derived malignancies. Expressed in thymus and spleen.3 Publications

Inductioni

Up-regulated by IL4/interleukin-4.1 Publication

Gene expression databases

BgeeiENSG00000174500
CleanExiHS_GAL
HS_GCET2
ExpressionAtlasiQ8N6F7 baseline and differential
GenevisibleiQ8N6F7 HS

Organism-specific databases

HPAiHPA002473

Interactioni

Subunit structurei

Interacts with ACTB and MYH2; the interaction with MYH2 is increased by IL6-induced phosphorylation. Interacts (via C-terminus) with ARHGEF11 (via DH domain). Interacts with ARHGEF12. Interacts with SYK; the interaction increases after B-cell receptor stimulation, resulting in enhanced SYK autophosphorylation and activity.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APPBP2Q926245EBI-10267082,EBI-743771

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • myosin II binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi129200, 1 interactor
IntActiQ8N6F7, 4 interactors
STRINGi9606.ENSP00000419485

Structurei

3D structure databases

ProteinModelPortaliQ8N6F7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG410JBYS Eukaryota
ENOG41115IK LUCA
GeneTreeiENSGT00730000111441
HOGENOMiHOG000293224
HOVERGENiHBG081549
InParanoidiQ8N6F7
OMAiEGCFCLP
OrthoDBiEOG091G0O8J
PhylomeDBiQ8N6F7
TreeFamiTF338596

Family and domain databases

InterProiView protein in InterPro
IPR031364 GC_assoc_lym
PANTHERiPTHR35351 PTHR35351, 1 hit
PfamiView protein in Pfam
PF15666 HGAL, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N6F7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNSLLRENR RQQNTQEMPW NVRMQSPKQR TSRCWDHHIA EGCFCLPWKK
60 70 80 90 100
ILIFEKRQDS QNENERMSST PIQDNVDQTY SEELCYTLIN HRVLCTRPSG
110 120 130 140 150
NSAEEYYENV PCKAERPRES LGGTETEYSL LHMPSTDPRH ARSPEDEYEL
160 170
LMPHRISSHF LQQPRPLMAP SETQFSHL
Length:178
Mass (Da):21,005
Last modified:October 1, 2002 - v1
Checksum:iB7C91F3D4B78CD03
GO
Isoform 2 (identifier: Q8N6F7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     9-9: N → NSF

Note: No experimental confirmation available.
Show »
Length:180
Mass (Da):21,240
Checksum:iF28DC1F48452F889
GO
Isoform 3 (identifier: Q8N6F7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     34-48: Missing.

Note: Gene prediction based on EST data.
Show »
Length:163
Mass (Da):19,206
Checksum:i482C190FAF5BFCA5
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0460859N → NSF in isoform 2. 1 Publication1
Alternative sequenceiVSP_04698434 – 48Missing in isoform 3. CuratedAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY212246 mRNA Translation: AAO22147.1
AF521911 mRNA Translation: AAO21701.1
AC128688 Genomic DNA No translation available.
BC030506 mRNA Translation: AAH30506.1
BM456595 mRNA No translation available.
CCDSiCCDS2964.1 [Q8N6F7-1]
CCDS54621.1 [Q8N6F7-3]
CCDS54622.1 [Q8N6F7-2]
RefSeqiNP_001177188.1, NM_001190259.1 [Q8N6F7-2]
NP_001177189.1, NM_001190260.1 [Q8N6F7-3]
NP_689998.1, NM_152785.4 [Q8N6F7-1]
UniGeneiHs.49614

Genome annotation databases

EnsembliENST00000308910; ENSP00000309487; ENSG00000174500 [Q8N6F7-1]
ENST00000460387; ENSP00000420603; ENSG00000174500 [Q8N6F7-3]
ENST00000484193; ENSP00000419485; ENSG00000174500 [Q8N6F7-2]
ENST00000643065; ENSP00000493535; ENSG00000284925 [Q8N6F7-2]
ENST00000644247; ENSP00000494547; ENSG00000284925 [Q8N6F7-3]
ENST00000646639; ENSP00000496265; ENSG00000284925 [Q8N6F7-1]
GeneIDi257144
KEGGihsa:257144
UCSCiuc003dys.2 human [Q8N6F7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGCSAM_HUMAN
AccessioniPrimary (citable) accession number: Q8N6F7
Secondary accession number(s): C9JD17, C9JUG6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 1, 2002
Last modified: July 18, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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