UniProtKB - Q8N6F7 (GCSAM_HUMAN)
Germinal center-associated signaling and motility protein
GCSAM
Functioni
GO - Molecular functioni
- actin binding Source: UniProtKB
- myosin II binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
GO - Biological processi
- negative regulation of lymphocyte migration Source: UniProtKB
- regulation of B cell receptor signaling pathway Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | Q8N6F7 |
Names & Taxonomyi
Protein namesi | Recommended name: Germinal center-associated signaling and motility proteinAlternative name(s): Germinal center B-cell-expressed transcript 2 protein Germinal center-associated lymphoma protein Short name: hGAL |
Gene namesi | Name:GCSAM Synonyms:GAL, GCET2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000174500.12 |
HGNCi | HGNC:20253, GCSAM |
MIMi | 607792, gene |
neXtProti | NX_Q8N6F7 |
Subcellular locationi
Plasma membrane
Other locations
Note: It relocalizes from the cytoplasm to podosome-like structures upon cell treatment with IL6.
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 106 – 107 | YY → AA: Does not affect the interaction with SYK. 1 Publication | 2 | |
Mutagenesisi | 128 | Y → F: Does not affect IL6 induced phosphorylation. Does not affect the interaction with SYK. 2 Publications | 1 | |
Mutagenesisi | 148 | Y → F: Prevents IL6 induced phosphorylation. Does not affect the interaction with SYK. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 257144 |
OpenTargetsi | ENSG00000174500 |
PharmGKBi | PA134980592 |
Miscellaneous databases
Pharosi | Q8N6F7, Tbio |
Polymorphism and mutation databases
BioMutai | GCSAM |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000256228 | 1 – 178 | Germinal center-associated signaling and motility proteinAdd BLAST | 178 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 99 | PhosphoserineBy similarity | 1 | |
Modified residuei | 148 | PhosphotyrosineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MassIVEi | Q8N6F7 |
MaxQBi | Q8N6F7 |
PaxDbi | Q8N6F7 |
PeptideAtlasi | Q8N6F7 |
PRIDEi | Q8N6F7 |
ProteomicsDBi | 11728 72164 [Q8N6F7-1] 9668 |
PTM databases
iPTMneti | Q8N6F7 |
PhosphoSitePlusi | Q8N6F7 |
SwissPalmi | Q8N6F7 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000174500, Expressed in tonsil and 110 other tissues |
ExpressionAtlasi | Q8N6F7, baseline and differential |
Genevisiblei | Q8N6F7, HS |
Organism-specific databases
HPAi | ENSG00000174500, Tissue enhanced (lymphoid tissue, skin) |
Interactioni
Subunit structurei
Interacts with ACTB and MYH2; the interaction with MYH2 is increased by IL6-induced phosphorylation.
Interacts (via C-terminus) with ARHGEF11 (via DH domain).
Interacts with ARHGEF12.
Interacts with SYK; the interaction increases after B-cell receptor stimulation, resulting in enhanced SYK autophosphorylation and activity.
3 PublicationsBinary interactionsi
Hide detailsQ8N6F7
With | #Exp. | IntAct |
---|---|---|
AP1M1 [Q9BXS5] | 3 | EBI-10267082,EBI-541426 |
APPBP2 [Q92624] | 6 | EBI-10267082,EBI-743771 |
CNOT2 [Q9NZN8] | 3 | EBI-10267082,EBI-743033 |
CYSRT1 [A8MQ03] | 3 | EBI-10267082,EBI-3867333 |
GO - Molecular functioni
- actin binding Source: UniProtKB
- myosin II binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 129200, 4 interactors |
IntActi | Q8N6F7, 4 interactors |
STRINGi | 9606.ENSP00000419485 |
Miscellaneous databases
RNActi | Q8N6F7, protein |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502TDUK, Eukaryota |
GeneTreei | ENSGT00940000158134 |
HOGENOMi | CLU_126867_0_0_1 |
InParanoidi | Q8N6F7 |
OMAi | CLPWKKM |
OrthoDBi | 1401343at2759 |
PhylomeDBi | Q8N6F7 |
TreeFami | TF338596 |
Family and domain databases
InterProi | View protein in InterPro IPR031364, GC_assoc_lym |
PANTHERi | PTHR35351, PTHR35351, 1 hit |
Pfami | View protein in Pfam PF15666, HGAL, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGNSLLRENR RQQNTQEMPW NVRMQSPKQR TSRCWDHHIA EGCFCLPWKK
60 70 80 90 100
ILIFEKRQDS QNENERMSST PIQDNVDQTY SEELCYTLIN HRVLCTRPSG
110 120 130 140 150
NSAEEYYENV PCKAERPRES LGGTETEYSL LHMPSTDPRH ARSPEDEYEL
160 170
LMPHRISSHF LQQPRPLMAP SETQFSHL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF2Z3F7 | F2Z3F7_HUMAN | Germinal center-associated-signalin... | GCSAM | 47 | Annotation score: | ||
C9JY41 | C9JY41_HUMAN | Germinal center-associated-signalin... | GCSAM | 126 | Annotation score: | ||
C9IY73 | C9IY73_HUMAN | Germinal center-associated-signalin... | GCSAM | 111 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046085 | 9 | N → NSF in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_046984 | 34 – 48 | Missing in isoform 3. CuratedAdd BLAST | 15 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY212246 mRNA Translation: AAO22147.1 AF521911 mRNA Translation: AAO21701.1 AC128688 Genomic DNA No translation available. BC030506 mRNA Translation: AAH30506.1 BM456595 mRNA No translation available. |
CCDSi | CCDS2964.1 [Q8N6F7-1] CCDS54621.1 [Q8N6F7-3] CCDS54622.1 [Q8N6F7-2] |
RefSeqi | NP_001177188.1, NM_001190259.1 [Q8N6F7-2] NP_001177189.1, NM_001190260.1 [Q8N6F7-3] NP_689998.1, NM_152785.4 [Q8N6F7-1] |
Genome annotation databases
Ensembli | ENST00000308910; ENSP00000309487; ENSG00000174500 [Q8N6F7-1] ENST00000460387; ENSP00000420603; ENSG00000174500 [Q8N6F7-3] ENST00000484193; ENSP00000419485; ENSG00000174500 [Q8N6F7-2] ENST00000643065; ENSP00000493535; ENSG00000284925 [Q8N6F7-2] ENST00000644247; ENSP00000494547; ENSG00000284925 [Q8N6F7-3] ENST00000646639; ENSP00000496265; ENSG00000284925 [Q8N6F7-1] |
GeneIDi | 257144 |
KEGGi | hsa:257144 |
UCSCi | uc003dys.2, human [Q8N6F7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY212246 mRNA Translation: AAO22147.1 AF521911 mRNA Translation: AAO21701.1 AC128688 Genomic DNA No translation available. BC030506 mRNA Translation: AAH30506.1 BM456595 mRNA No translation available. |
CCDSi | CCDS2964.1 [Q8N6F7-1] CCDS54621.1 [Q8N6F7-3] CCDS54622.1 [Q8N6F7-2] |
RefSeqi | NP_001177188.1, NM_001190259.1 [Q8N6F7-2] NP_001177189.1, NM_001190260.1 [Q8N6F7-3] NP_689998.1, NM_152785.4 [Q8N6F7-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 129200, 4 interactors |
IntActi | Q8N6F7, 4 interactors |
STRINGi | 9606.ENSP00000419485 |
PTM databases
iPTMneti | Q8N6F7 |
PhosphoSitePlusi | Q8N6F7 |
SwissPalmi | Q8N6F7 |
Polymorphism and mutation databases
BioMutai | GCSAM |
Proteomic databases
MassIVEi | Q8N6F7 |
MaxQBi | Q8N6F7 |
PaxDbi | Q8N6F7 |
PeptideAtlasi | Q8N6F7 |
PRIDEi | Q8N6F7 |
ProteomicsDBi | 11728 72164 [Q8N6F7-1] 9668 |
Protocols and materials databases
Antibodypediai | 1196, 164 antibodies |
DNASUi | 257144 |
Genome annotation databases
Ensembli | ENST00000308910; ENSP00000309487; ENSG00000174500 [Q8N6F7-1] ENST00000460387; ENSP00000420603; ENSG00000174500 [Q8N6F7-3] ENST00000484193; ENSP00000419485; ENSG00000174500 [Q8N6F7-2] ENST00000643065; ENSP00000493535; ENSG00000284925 [Q8N6F7-2] ENST00000644247; ENSP00000494547; ENSG00000284925 [Q8N6F7-3] ENST00000646639; ENSP00000496265; ENSG00000284925 [Q8N6F7-1] |
GeneIDi | 257144 |
KEGGi | hsa:257144 |
UCSCi | uc003dys.2, human [Q8N6F7-1] |
Organism-specific databases
CTDi | 257144 |
DisGeNETi | 257144 |
EuPathDBi | HostDB:ENSG00000174500.12 |
GeneCardsi | GCSAM |
HGNCi | HGNC:20253, GCSAM |
HPAi | ENSG00000174500, Tissue enhanced (lymphoid tissue, skin) |
MIMi | 607792, gene |
neXtProti | NX_Q8N6F7 |
OpenTargetsi | ENSG00000174500 |
PharmGKBi | PA134980592 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502TDUK, Eukaryota |
GeneTreei | ENSGT00940000158134 |
HOGENOMi | CLU_126867_0_0_1 |
InParanoidi | Q8N6F7 |
OMAi | CLPWKKM |
OrthoDBi | 1401343at2759 |
PhylomeDBi | Q8N6F7 |
TreeFami | TF338596 |
Enzyme and pathway databases
PathwayCommonsi | Q8N6F7 |
Miscellaneous databases
BioGRID-ORCSi | 257144, 4 hits in 822 CRISPR screens |
GenomeRNAii | 257144 |
Pharosi | Q8N6F7, Tbio |
PROi | PR:Q8N6F7 |
RNActi | Q8N6F7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000174500, Expressed in tonsil and 110 other tissues |
ExpressionAtlasi | Q8N6F7, baseline and differential |
Genevisiblei | Q8N6F7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR031364, GC_assoc_lym |
PANTHERi | PTHR35351, PTHR35351, 1 hit |
Pfami | View protein in Pfam PF15666, HGAL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GCSAM_HUMAN | |
Accessioni | Q8N6F7Primary (citable) accession number: Q8N6F7 Secondary accession number(s): C9JD17, C9JUG6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot