UniProtKB - Q8N6D2 (RN182_HUMAN)
Protein
E3 ubiquitin-protein ligase RNF182
Gene
RNF182
Organism
Homo sapiens (Human)
Status
Functioni
E3 ubiquitin-protein ligase that mediates the ubiquitination of ATP6V0C and targets it to degradation via the ubiquitin-proteasome pathway (PubMed:18298843). Plays also a role in the inhibition of TLR-triggered innate immune response by mediating 'Lys'-48-linked ubiquitination and subsequent degradation of NF-kappa-B component RELA (PubMed:31432514).2 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 20 – 68 | RING-typePROSITE-ProRule annotationAdd BLAST | 49 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- protein ubiquitination Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8N6D2 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RNF1821 Publication (EC:2.3.2.271 Publication)Alternative name(s): RING finger protein 182 RING-type E3 ubiquitin transferase RNF182Curated |
Gene namesi | Name:RNF182 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28522, RNF182 |
neXtProti | NX_Q8N6D2 |
VEuPathDBi | HostDB:ENSG00000180537.12 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
- Cytoplasm 2 Publications
Other locations
- cytoplasm Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 184 – 204 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 211 – 231 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cytoplasm, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 221687 |
OpenTargetsi | ENSG00000180537 |
PharmGKBi | PA134975171 |
Miscellaneous databases
Pharosi | Q8N6D2, Tdark |
Genetic variation databases
BioMutai | RNF182 |
DMDMi | 74751050 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000261620 | 1 – 247 | E3 ubiquitin-protein ligase RNF182Add BLAST | 247 |
Proteomic databases
EPDi | Q8N6D2 |
jPOSTi | Q8N6D2 |
PaxDbi | Q8N6D2 |
PeptideAtlasi | Q8N6D2 |
PRIDEi | Q8N6D2 |
ProteomicsDBi | 72159 |
PTM databases
iPTMneti | Q8N6D2 |
PhosphoSitePlusi | Q8N6D2 |
Expressioni
Tissue specificityi
Up-regulated in neuronal cells subjected to cell death-inducing injuries, such as oxygen and glucose deprivation (at protein level). Could be up-regulated in Alzheimer disease brains (PubMed:18298843). Highly expressed in innate immune organs such as lymph nodes and spleen and in immune cells such as macrophages and dendritic cells (PubMed:31432514).2 Publications
Gene expression databases
Bgeei | ENSG00000180537, Expressed in neocortex and 155 other tissues |
ExpressionAtlasi | Q8N6D2, baseline and differential |
Genevisiblei | Q8N6D2, HS |
Organism-specific databases
HPAi | ENSG00000180537, Tissue enhanced (bone marrow, brain) |
Interactioni
Subunit structurei
Interacts with ATP6V0C.
1 PublicationBinary interactionsi
Hide detailsQ8N6D2
With | #Exp. | IntAct |
---|---|---|
UBE2D1 [P51668] | 3 | EBI-2130099,EBI-743540 |
UBE2K [P61086] | 3 | EBI-2130099,EBI-473850 |
UBE2L3 [P68036] | 3 | EBI-2130099,EBI-711173 |
UBE2N [P61088] | 2 | EBI-2130099,EBI-1052908 |
Protein-protein interaction databases
BioGRIDi | 128745, 7 interactors |
IntActi | Q8N6D2, 6 interactors |
STRINGi | 9606.ENSP00000420465 |
Miscellaneous databases
RNActi | Q8N6D2, protein |
Family & Domainsi
Domaini
The RING-type zinc finger domain is required for E3 ligase activity.By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 20 – 68 | RING-typePROSITE-ProRule annotationAdd BLAST | 49 |
Keywords - Domaini
Transmembrane, Transmembrane helix, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00730000111020 |
HOGENOMi | CLU_100624_0_0_1 |
InParanoidi | Q8N6D2 |
OMAi | FLDCMAL |
OrthoDBi | 1216224at2759 |
PhylomeDBi | Q8N6D2 |
TreeFami | TF331690 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR042285, RNF182 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR46675, PTHR46675, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q8N6D2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASQPPEDTA ESQASDELEC KICYNRYNLK QRKPKVLECC HRVCAKCLYK
60 70 80 90 100
IIDFGDSPQG VIVCPFCRFE TCLPDDEVSS LPDDNNILVN LTCGGKGKKC
110 120 130 140 150
LPENPTELLL TPKRLASLVS PSHTSSNCLV ITIMEVQRES SPSLSSTPVV
160 170 180 190 200
EFYRPASFDS VTTVSHNWTV WNCTSLLFQT SIRVLVWLLG LLYFSSLPLG
210 220 230 240
IYLLVSKKVT LGVVFVSLVP SSLVILMVYG FCQCVCHEFL DCMAPPS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JVS8 | C9JVS8_HUMAN | E3 ubiquitin-protein ligase RNF182 | RNF182 | 162 | Annotation score: | ||
C9IZ30 | C9IZ30_HUMAN | E3 ubiquitin-protein ligase RNF182 | RNF182 | 82 | Annotation score: | ||
C9JAK6 | C9JAK6_HUMAN | RING-type E3 ubiquitin transferase | RNF182 | 51 | Annotation score: | ||
C9JAJ3 | C9JAJ3_HUMAN | E3 ubiquitin-protein ligase RNF182 | RNF182 | 13 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 108 | L → P in BAC03481 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 139 | E → K in BAC03481 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035959 | 43 | V → A in a colorectal cancer sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_035960 | 58 | P → L in a colorectal cancer sample; somatic mutation. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK090576 mRNA Translation: BAC03481.1 AK315528 mRNA Translation: BAG37909.1 AL138718 Genomic DNA No translation available. CH471087 Genomic DNA Translation: EAW55349.1 BC030666 mRNA Translation: AAH30666.1 BC050030 mRNA Translation: AAH50030.1 |
CCDSi | CCDS4531.1 |
RefSeqi | NP_001158504.1, NM_001165032.1 NP_001158505.1, NM_001165033.1 NP_001158506.1, NM_001165034.1 NP_689950.1, NM_152737.3 XP_016865937.1, XM_017010448.1 XP_016865938.1, XM_017010449.1 XP_016865939.1, XM_017010450.1 |
Genome annotation databases
Ensembli | ENST00000488300; ENSP00000420465; ENSG00000180537 ENST00000537388; ENSP00000441271; ENSG00000180537 ENST00000537663; ENSP00000443228; ENSG00000180537 ENST00000544682; ENSP00000442021; ENSG00000180537 |
GeneIDi | 221687 |
KEGGi | hsa:221687 |
UCSCi | uc003nbe.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK090576 mRNA Translation: BAC03481.1 AK315528 mRNA Translation: BAG37909.1 AL138718 Genomic DNA No translation available. CH471087 Genomic DNA Translation: EAW55349.1 BC030666 mRNA Translation: AAH30666.1 BC050030 mRNA Translation: AAH50030.1 |
CCDSi | CCDS4531.1 |
RefSeqi | NP_001158504.1, NM_001165032.1 NP_001158505.1, NM_001165033.1 NP_001158506.1, NM_001165034.1 NP_689950.1, NM_152737.3 XP_016865937.1, XM_017010448.1 XP_016865938.1, XM_017010449.1 XP_016865939.1, XM_017010450.1 |
3D structure databases
SMRi | Q8N6D2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 128745, 7 interactors |
IntActi | Q8N6D2, 6 interactors |
STRINGi | 9606.ENSP00000420465 |
PTM databases
iPTMneti | Q8N6D2 |
PhosphoSitePlusi | Q8N6D2 |
Genetic variation databases
BioMutai | RNF182 |
DMDMi | 74751050 |
Proteomic databases
EPDi | Q8N6D2 |
jPOSTi | Q8N6D2 |
PaxDbi | Q8N6D2 |
PeptideAtlasi | Q8N6D2 |
PRIDEi | Q8N6D2 |
ProteomicsDBi | 72159 |
Protocols and materials databases
Antibodypediai | 1547, 128 antibodies |
DNASUi | 221687 |
Genome annotation databases
Ensembli | ENST00000488300; ENSP00000420465; ENSG00000180537 ENST00000537388; ENSP00000441271; ENSG00000180537 ENST00000537663; ENSP00000443228; ENSG00000180537 ENST00000544682; ENSP00000442021; ENSG00000180537 |
GeneIDi | 221687 |
KEGGi | hsa:221687 |
UCSCi | uc003nbe.4, human |
Organism-specific databases
CTDi | 221687 |
DisGeNETi | 221687 |
GeneCardsi | RNF182 |
HGNCi | HGNC:28522, RNF182 |
HPAi | ENSG00000180537, Tissue enhanced (bone marrow, brain) |
neXtProti | NX_Q8N6D2 |
OpenTargetsi | ENSG00000180537 |
PharmGKBi | PA134975171 |
VEuPathDBi | HostDB:ENSG00000180537.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00730000111020 |
HOGENOMi | CLU_100624_0_0_1 |
InParanoidi | Q8N6D2 |
OMAi | FLDCMAL |
OrthoDBi | 1216224at2759 |
PhylomeDBi | Q8N6D2 |
TreeFami | TF331690 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q8N6D2 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 221687, 8 hits in 873 CRISPR screens |
ChiTaRSi | RNF182, human |
GenomeRNAii | 221687 |
Pharosi | Q8N6D2, Tdark |
PROi | PR:Q8N6D2 |
RNActi | Q8N6D2, protein |
Gene expression databases
Bgeei | ENSG00000180537, Expressed in neocortex and 155 other tissues |
ExpressionAtlasi | Q8N6D2, baseline and differential |
Genevisiblei | Q8N6D2, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR042285, RNF182 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR46675, PTHR46675, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RN182_HUMAN | |
Accessioni | Q8N6D2Primary (citable) accession number: Q8N6D2 Secondary accession number(s): B2RDG2, Q8NBG3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - PATHWAY comments
Index of metabolic and biosynthesis pathways - Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants