Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc finger CCCH domain-containing protein 8

Gene

ZC3H8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:23932780). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri191 – 218C3H1-type 1PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri220 – 247C3H1-type 2PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri248 – 271C3H1-type 3PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • negative regulation of T cell differentiation in thymus Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of thymocyte apoptotic process Source: UniProtKB
  • positive regulation of transcription by RNA polymerase III Source: UniProtKB
  • response to antibiotic Source: UniProtKB
  • snRNA transcription by RNA polymerase II Source: UniProtKB
  • snRNA transcription by RNA polymerase III Source: UniProtKB
  • T cell homeostasis Source: UniProtKB

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processApoptosis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-6807505 RNA polymerase II transcribes snRNA genes

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger CCCH domain-containing protein 8
Gene namesi
Name:ZC3H8
Synonyms:ZC3HDC8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000144161.12
HGNCiHGNC:30941 ZC3H8
neXtProtiNX_Q8N5P1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000144161
PharmGKBiPA134956342

Polymorphism and mutation databases

BioMutaiZC3H8
DMDMi47117585

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002139031 – 291Zinc finger CCCH domain-containing protein 8Add BLAST291

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22PhosphoserineCombined sources1
Modified residuei32PhosphothreonineCombined sources1
Cross-linki43Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei59PhosphoserineCombined sources1
Modified residuei63PhosphoserineCombined sources1
Modified residuei77PhosphoserineCombined sources1
Modified residuei83PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8N5P1
MaxQBiQ8N5P1
PaxDbiQ8N5P1
PeptideAtlasiQ8N5P1
PRIDEiQ8N5P1
ProteomicsDBi72080

PTM databases

iPTMnetiQ8N5P1
PhosphoSitePlusiQ8N5P1

Expressioni

Gene expression databases

BgeeiENSG00000144161
CleanExiHS_ZC3H8
ExpressionAtlasiQ8N5P1 baseline and differential
GenevisibleiQ8N5P1 HS

Organism-specific databases

HPAiHPA034803
HPA034804

Interactioni

Subunit structurei

Component of the little elongation complex (LEC), at least composed of ELL (ELL, ELL2 or ELL3), ZC3H8, ICE1 and ICE2. Interacts with ICE1 (via C-terminus domain).1 Publication

Protein-protein interaction databases

BioGridi124116, 24 interactors
IntActiQ8N5P1, 5 interactors
STRINGi9606.ENSP00000386488

Structurei

3D structure databases

ProteinModelPortaliQ8N5P1
SMRiQ8N5P1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region and all three C3H1-type zinc fingers are necessary to induce transcriptional repression.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri191 – 218C3H1-type 1PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri220 – 247C3H1-type 2PROSITE-ProRule annotationAdd BLAST28
Zinc fingeri248 – 271C3H1-type 3PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1040 Eukaryota
COG5084 LUCA
GeneTreeiENSGT00530000063288
HOGENOMiHOG000231732
HOVERGENiHBG057407
InParanoidiQ8N5P1
PhylomeDBiQ8N5P1
TreeFamiTF318143

Family and domain databases

InterProiView protein in InterPro
IPR000571 Znf_CCCH
IPR036855 Znf_CCCH_sf
PfamiView protein in Pfam
PF00642 zf-CCCH, 2 hits
SMARTiView protein in SMART
SM00356 ZnF_C3H1, 3 hits
SUPFAMiSSF90229 SSF90229, 3 hits
PROSITEiView protein in PROSITE
PS50103 ZF_C3H1, 3 hits

Sequencei

Sequence statusi: Complete.

Q8N5P1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFENLFSKP PNPALGKTAT DSDERIDDEI DTEVEETQEE KIKLECEQIP
60 70 80 90 100
KKFRHSAISP KSSLHRKSRS KDYDVYSDND ICSQESEDNF AKELQQYIQA
110 120 130 140 150
REMANAAQPE ESTKKEGVKD TPQAAKQKNK NLKAGHKNGK QKKMKRKWPG
160 170 180 190 200
PGNKGSNALL RNSGSQEEDG KPKEKQQHLS QAFINQHTVE RKGKQICKYF
210 220 230 240 250
LERKCIKGDQ CKFDHDAEIE KKKEMCKFYV QGYCTRGENC LYLHNEYPCK
260 270 280 290
FYHTGTKCYQ GEYCKFSHAP LTPETQELLA KVLDTEKKSC K
Length:291
Mass (Da):33,576
Last modified:May 10, 2004 - v2
Checksum:i5DFDA42280916034
GO

Sequence cautioni

The sequence AAH32001 differs from that shown. Reason: Frameshift at position 291.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti233 – 235YCT → FCS in AAK13496 (Ref. 1) Curated3
Sequence conflicti265K → Q in AAK13496 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057489240C → W. Corresponds to variant dbSNP:rs35782954Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334161 mRNA Translation: AAK13496.1
BC032001 mRNA Translation: AAH32001.1 Frameshift.
CCDSiCCDS46392.1
RefSeqiNP_115883.2, NM_032494.2
UniGeneiHs.418416

Genome annotation databases

EnsembliENST00000272570; ENSP00000272570; ENSG00000144161
ENST00000409573; ENSP00000386488; ENSG00000144161
GeneIDi84524
KEGGihsa:84524
UCSCiuc032nyp.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiZC3H8_HUMAN
AccessioniPrimary (citable) accession number: Q8N5P1
Secondary accession number(s): Q9BZ75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: July 18, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health