UniProtKB - Q8N5A5 (ZGPAT_HUMAN)
Protein
Zinc finger CCCH-type with G patch domain-containing protein
Gene
ZGPAT
Organism
Homo sapiens (Human)
Status
Functioni
Transcription repressor that specifically binds the 5'-GGAG[GA]A[GA]A-3' consensus sequence. Represses transcription by recruiting the chromatin multiprotein complex NuRD to target promoters. Negatively regulates expression of EGFR, a gene involved in cell proliferation, survival and migration. Its ability to repress genes of the EGFR pathway suggest it may act as a tumor suppressor. Able to suppress breast carcinogenesis.1 Publication
Antagonizes the transcription repression by isoform 1 by competing for the binding of the NuRD complex. Does not bind DNA.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 175 – 201 | C3H1-typePROSITE-ProRule annotationAdd BLAST | 27 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- negative regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q8N5A5 |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger CCCH-type with G patch domain-containing proteinAlternative name(s): G patch domain-containing protein 6 Zinc finger CCCH domain-containing protein 9 Zinc finger and G patch domain-containing protein |
Gene namesi | Name:ZGPAT Synonyms:GPATC6, GPATCH6, KIAA1847, ZC3H9, ZC3HDC9, ZIP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:15948, ZGPAT |
neXtProti | NX_Q8N5A5 |
VEuPathDBi | HostDB:ENSG00000197114.11 |
Subcellular locationi
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- chromatin Source: NTNU_SB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 84619 |
OpenTargetsi | ENSG00000197114 |
PharmGKBi | PA134881248 |
Miscellaneous databases
Pharosi | Q8N5A5, Tbio |
Genetic variation databases
BioMutai | ZGPAT |
DMDMi | 147744602 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213894 | 1 – 531 | Zinc finger CCCH-type with G patch domain-containing proteinAdd BLAST | 531 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 373 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Ubiquitinated in case of infection by HIV-1, leading to its degradation. Ubiquitination is mediated by the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex that is hijacked by HIV-1 via interaction between HIV-1 Vpr and DCAF1/VPRBP.1 Publication
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8N5A5 |
jPOSTi | Q8N5A5 |
MassIVEi | Q8N5A5 |
MaxQBi | Q8N5A5 |
PaxDbi | Q8N5A5 |
PeptideAtlasi | Q8N5A5 |
PRIDEi | Q8N5A5 |
ProteomicsDBi | 72025 [Q8N5A5-1] 72026 [Q8N5A5-2] 72027 [Q8N5A5-3] |
PTM databases
iPTMneti | Q8N5A5 |
PhosphoSitePlusi | Q8N5A5 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Inductioni
Down-regulated in breast carcinomas.
Gene expression databases
Bgeei | ENSG00000197114, Expressed in liver and 117 other tissues |
ExpressionAtlasi | Q8N5A5, baseline and differential |
Genevisiblei | Q8N5A5, HS |
Organism-specific databases
HPAi | ENSG00000197114, Tissue enhanced (liver) |
Interactioni
Subunit structurei
Interacts with CHD4/Mi-2; the interaction is direct.
2 PublicationsBinary interactionsi
Q8N5A5
Isoform 2 [Q8N5A5-2]
Protein-protein interaction databases
BioGRIDi | 124150, 136 interactors |
IntActi | Q8N5A5, 113 interactors |
MINTi | Q8N5A5 |
STRINGi | 9606.ENSP00000332013 |
Miscellaneous databases
RNActi | Q8N5A5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8N5A5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 333 – 379 | G-patchPROSITE-ProRule annotationAdd BLAST | 47 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 119 – 128 | Poly-Glu | 10 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 175 – 201 | C3H1-typePROSITE-ProRule annotationAdd BLAST | 27 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2185, Eukaryota |
GeneTreei | ENSGT00390000000732 |
HOGENOMi | CLU_040504_1_0_1 |
InParanoidi | Q8N5A5 |
OMAi | QMGYEFG |
OrthoDBi | 1238995at2759 |
PhylomeDBi | Q8N5A5 |
TreeFami | TF105970 |
Family and domain databases
CDDi | cd04508, TUDOR, 1 hit |
InterProi | View protein in InterPro IPR000467, G_patch_dom IPR002999, Tudor IPR043560, ZGPAT IPR041367, Znf-CCCH_4 IPR000571, Znf_CCCH IPR036855, Znf_CCCH_sf |
PANTHERi | PTHR46297, PTHR46297, 1 hit |
Pfami | View protein in Pfam PF01585, G-patch, 1 hit PF18044, zf-CCCH_4, 1 hit |
SMARTi | View protein in SMART SM00443, G_patch, 1 hit SM00356, ZnF_C3H1, 1 hit |
SUPFAMi | SSF90229, SSF90229, 1 hit |
PROSITEi | View protein in PROSITE PS50174, G_PATCH, 1 hit PS50103, ZF_C3H1, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8N5A5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDEESLESAL QTYRAQLQQV ELALGAGLDS SEQADLRQLQ GDLKELIELT
60 70 80 90 100
EASLVSVRKS SLLAALDEER PGRQEDAEYQ AFREAITEAV EAPAAARGSG
110 120 130 140 150
SETVPKAEAG PESAAGGQEE EEGEDEEELS GTKVSAPYYS SWGTLEYHNA
160 170 180 190 200
MVVGTEEAED GSAGVRVLYL YPTHKSLKPC PFFLEGKCRF KENCRFSHGQ
210 220 230 240 250
VVSLDELRPF QDPDLSSLQA GSACLAKHQD GLWHAARITD VDNGYYTVKF
260 270 280 290 300
DSLLLREAVV EGDGILPPLR TEATESDSDS DGTGDSSYAR VVGSDAVDSA
310 320 330 340 350
QSSALCPSLA VVGSDAVDSG TCSSAFAGWE VHTRGIGSRL LTKMGYEFGK
360 370 380 390 400
GLGRHAEGRV EPIHAVVLPR GKSLDQCVET LQKQTRVGKA GTNKPPRCRG
410 420 430 440 450
RGARPGGRPA PRNVFDFLNE KLQGQAPGAL EAGAAPAGRR SKDMYHASKS
460 470 480 490 500
AKRALSLRLF QTEEKIERTQ RDIRSIQEAL ARNAGRHSVA SAQLQEKLAG
510 520 530
AQRQLGQLRA QEAGLQQEQR KADTHKKMTE F
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketX6RGY1 | X6RGY1_HUMAN | Zinc finger CCCH-type with G patch ... | ZGPAT | 97 | Annotation score: |
Sequence cautioni
The sequence BAB47476 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAC11317 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 184 | L → Q in BAB55426 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 188 | C → R in BAC11317 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 344 | M → V in BAB55426 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 433 | G → R in AAH19338 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 519 | Q → R in BAB55426 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_025539 | 61 | S → R4 PublicationsCorresponds to variant dbSNP:rs1291212Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053599 | 1 – 343 | Missing in isoform 4. CuratedAdd BLAST | 343 | |
Alternative sequenceiVSP_038121 | 282 – 310 | Missing in isoform 3. 1 PublicationAdd BLAST | 29 | |
Alternative sequenceiVSP_007754 | 291 – 310 | Missing in isoform 2. 2 PublicationsAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB058750 mRNA Translation: BAB47476.3 Different initiation. AK027878 mRNA Translation: BAB55426.1 AK074961 mRNA Translation: BAC11317.1 Frameshift. AL121845 Genomic DNA No translation available. CH471077 Genomic DNA Translation: EAW75223.1 CH471077 Genomic DNA Translation: EAW75226.1 BC019338 mRNA Translation: AAH19338.1 BC032612 mRNA Translation: AAH32612.1 |
CCDSi | CCDS13534.1 [Q8N5A5-1] CCDS13535.1 [Q8N5A5-2] CCDS56203.1 [Q8N5A5-3] |
RefSeqi | NP_001076582.1, NM_001083113.1 [Q8N5A5-2] NP_001182582.1, NM_001195653.1 [Q8N5A5-2] NP_001182583.1, NM_001195654.1 [Q8N5A5-3] NP_115916.3, NM_032527.4 [Q8N5A5-1] NP_852150.2, NM_181485.2 [Q8N5A5-2] |
Genome annotation databases
Ensembli | ENST00000328969; ENSP00000332013; ENSG00000197114 [Q8N5A5-1] ENST00000355969; ENSP00000348242; ENSG00000197114 [Q8N5A5-2] ENST00000357119; ENSP00000349634; ENSG00000197114 [Q8N5A5-3] ENST00000369967; ENSP00000358984; ENSG00000197114 [Q8N5A5-2] ENST00000448100; ENSP00000391176; ENSG00000197114 [Q8N5A5-2] |
GeneIDi | 84619 |
KEGGi | hsa:84619 |
UCSCi | uc002ygi.3, human [Q8N5A5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB058750 mRNA Translation: BAB47476.3 Different initiation. AK027878 mRNA Translation: BAB55426.1 AK074961 mRNA Translation: BAC11317.1 Frameshift. AL121845 Genomic DNA No translation available. CH471077 Genomic DNA Translation: EAW75223.1 CH471077 Genomic DNA Translation: EAW75226.1 BC019338 mRNA Translation: AAH19338.1 BC032612 mRNA Translation: AAH32612.1 |
CCDSi | CCDS13534.1 [Q8N5A5-1] CCDS13535.1 [Q8N5A5-2] CCDS56203.1 [Q8N5A5-3] |
RefSeqi | NP_001076582.1, NM_001083113.1 [Q8N5A5-2] NP_001182582.1, NM_001195653.1 [Q8N5A5-2] NP_001182583.1, NM_001195654.1 [Q8N5A5-3] NP_115916.3, NM_032527.4 [Q8N5A5-1] NP_852150.2, NM_181485.2 [Q8N5A5-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4II1 | X-ray | 2.65 | A/B/C/D | 120-268 | [»] | |
SMRi | Q8N5A5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124150, 136 interactors |
IntActi | Q8N5A5, 113 interactors |
MINTi | Q8N5A5 |
STRINGi | 9606.ENSP00000332013 |
PTM databases
iPTMneti | Q8N5A5 |
PhosphoSitePlusi | Q8N5A5 |
Genetic variation databases
BioMutai | ZGPAT |
DMDMi | 147744602 |
Proteomic databases
EPDi | Q8N5A5 |
jPOSTi | Q8N5A5 |
MassIVEi | Q8N5A5 |
MaxQBi | Q8N5A5 |
PaxDbi | Q8N5A5 |
PeptideAtlasi | Q8N5A5 |
PRIDEi | Q8N5A5 |
ProteomicsDBi | 72025 [Q8N5A5-1] 72026 [Q8N5A5-2] 72027 [Q8N5A5-3] |
Protocols and materials databases
ABCDi | Q8N5A5, 5 sequenced antibodies |
Antibodypediai | 29857, 113 antibodies |
DNASUi | 84619 |
Genome annotation databases
Ensembli | ENST00000328969; ENSP00000332013; ENSG00000197114 [Q8N5A5-1] ENST00000355969; ENSP00000348242; ENSG00000197114 [Q8N5A5-2] ENST00000357119; ENSP00000349634; ENSG00000197114 [Q8N5A5-3] ENST00000369967; ENSP00000358984; ENSG00000197114 [Q8N5A5-2] ENST00000448100; ENSP00000391176; ENSG00000197114 [Q8N5A5-2] |
GeneIDi | 84619 |
KEGGi | hsa:84619 |
UCSCi | uc002ygi.3, human [Q8N5A5-1] |
Organism-specific databases
CTDi | 84619 |
DisGeNETi | 84619 |
GeneCardsi | ZGPAT |
HGNCi | HGNC:15948, ZGPAT |
HPAi | ENSG00000197114, Tissue enhanced (liver) |
neXtProti | NX_Q8N5A5 |
OpenTargetsi | ENSG00000197114 |
PharmGKBi | PA134881248 |
VEuPathDBi | HostDB:ENSG00000197114.11 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2185, Eukaryota |
GeneTreei | ENSGT00390000000732 |
HOGENOMi | CLU_040504_1_0_1 |
InParanoidi | Q8N5A5 |
OMAi | QMGYEFG |
OrthoDBi | 1238995at2759 |
PhylomeDBi | Q8N5A5 |
TreeFami | TF105970 |
Enzyme and pathway databases
PathwayCommonsi | Q8N5A5 |
Miscellaneous databases
BioGRID-ORCSi | 84619, 14 hits in 1001 CRISPR screens |
ChiTaRSi | ZGPAT, human |
GeneWikii | ZGPAT |
GenomeRNAii | 84619 |
Pharosi | Q8N5A5, Tbio |
PROi | PR:Q8N5A5 |
RNActi | Q8N5A5, protein |
Gene expression databases
Bgeei | ENSG00000197114, Expressed in liver and 117 other tissues |
ExpressionAtlasi | Q8N5A5, baseline and differential |
Genevisiblei | Q8N5A5, HS |
Family and domain databases
CDDi | cd04508, TUDOR, 1 hit |
InterProi | View protein in InterPro IPR000467, G_patch_dom IPR002999, Tudor IPR043560, ZGPAT IPR041367, Znf-CCCH_4 IPR000571, Znf_CCCH IPR036855, Znf_CCCH_sf |
PANTHERi | PTHR46297, PTHR46297, 1 hit |
Pfami | View protein in Pfam PF01585, G-patch, 1 hit PF18044, zf-CCCH_4, 1 hit |
SMARTi | View protein in SMART SM00443, G_patch, 1 hit SM00356, ZnF_C3H1, 1 hit |
SUPFAMi | SSF90229, SSF90229, 1 hit |
PROSITEi | View protein in PROSITE PS50174, G_PATCH, 1 hit PS50103, ZF_C3H1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZGPAT_HUMAN | |
Accessioni | Q8N5A5Primary (citable) accession number: Q8N5A5 Secondary accession number(s): E1P5K1 Q9H401 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2003 |
Last sequence update: | May 15, 2007 | |
Last modified: | April 7, 2021 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PDB cross-references
Index of Protein Data Bank (PDB) cross-references