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Protein

Zinc finger CCCH-type with G patch domain-containing protein

Gene

ZGPAT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription repressor that specifically binds the 5'-GGAG[GA]A[GA]A-3' consensus sequence. Represses transcription by recruiting the chromatin multiprotein complex NuRD to target promoters. Negatively regulates expression of EGFR, a gene involved in cell proliferation, survival and migration. Its ability to repress genes of the EGFR pathway suggest it may act as a tumor suppressor. Able to suppress breast carcinogenesis.1 Publication
Isoform 4: Antagonizes the transcription repression by isoform 1 by competing for the binding of the NuRD complex. Does not bind DNA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri175 – 201C3H1-typePROSITE-ProRule annotationAdd BLAST27

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger CCCH-type with G patch domain-containing protein
Alternative name(s):
G patch domain-containing protein 6
Zinc finger CCCH domain-containing protein 9
Zinc finger and G patch domain-containing protein
Gene namesi
Name:ZGPAT
Synonyms:GPATC6, GPATCH6, KIAA1847, ZC3H9, ZC3HDC9, ZIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000197114.11
HGNCiHGNC:15948 ZGPAT
neXtProtiNX_Q8N5A5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi84619
OpenTargetsiENSG00000197114
PharmGKBiPA134881248

Polymorphism and mutation databases

BioMutaiZGPAT
DMDMi147744602

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002138941 – 531Zinc finger CCCH-type with G patch domain-containing proteinAdd BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei373PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated in case of infection by HIV-1, leading to its degradation. Ubiquitination is mediated by the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex that is hijacked by HIV-1 via interaction between HIV-1 Vpr and DCAF1/VPRBP.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8N5A5
MaxQBiQ8N5A5
PaxDbiQ8N5A5
PeptideAtlasiQ8N5A5
PRIDEiQ8N5A5
ProteomicsDBi72025
72026 [Q8N5A5-2]
72027 [Q8N5A5-3]

PTM databases

iPTMnetiQ8N5A5
PhosphoSitePlusiQ8N5A5

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

Down-regulated in breast carcinomas.

Gene expression databases

BgeeiENSG00000197114 Expressed in 93 organ(s), highest expression level in liver
CleanExiHS_ZGPAT
ExpressionAtlasiQ8N5A5 baseline and differential
GenevisibleiQ8N5A5 HS

Organism-specific databases

HPAiHPA056705

Interactioni

Subunit structurei

Interacts with CHD4/Mi-2; the interaction is direct.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi124150, 61 interactors
IntActiQ8N5A5, 89 interactors
MINTiQ8N5A5
STRINGi9606.ENSP00000332013

Structurei

Secondary structure

1531
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8N5A5
SMRiQ8N5A5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini333 – 379G-patchPROSITE-ProRule annotationAdd BLAST47

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi119 – 128Poly-Glu10

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri175 – 201C3H1-typePROSITE-ProRule annotationAdd BLAST27

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2185 Eukaryota
ENOG410Z4ZI LUCA
GeneTreeiENSGT00390000000732
HOVERGENiHBG056371
InParanoidiQ8N5A5
OMAiRFKENCR
OrthoDBiEOG091G0FZN
PhylomeDBiQ8N5A5
TreeFamiTF105970

Family and domain databases

InterProiView protein in InterPro
IPR000467 G_patch_dom
IPR000571 Znf_CCCH
IPR036855 Znf_CCCH_sf
PfamiView protein in Pfam
PF01585 G-patch, 1 hit
SMARTiView protein in SMART
SM00443 G_patch, 1 hit
SM00356 ZnF_C3H1, 1 hit
SUPFAMiSSF90229 SSF90229, 1 hit
PROSITEiView protein in PROSITE
PS50174 G_PATCH, 1 hit
PS50103 ZF_C3H1, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: Q8N5A5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDEESLESAL QTYRAQLQQV ELALGAGLDS SEQADLRQLQ GDLKELIELT
60 70 80 90 100
EASLVSVRKS SLLAALDEER PGRQEDAEYQ AFREAITEAV EAPAAARGSG
110 120 130 140 150
SETVPKAEAG PESAAGGQEE EEGEDEEELS GTKVSAPYYS SWGTLEYHNA
160 170 180 190 200
MVVGTEEAED GSAGVRVLYL YPTHKSLKPC PFFLEGKCRF KENCRFSHGQ
210 220 230 240 250
VVSLDELRPF QDPDLSSLQA GSACLAKHQD GLWHAARITD VDNGYYTVKF
260 270 280 290 300
DSLLLREAVV EGDGILPPLR TEATESDSDS DGTGDSSYAR VVGSDAVDSA
310 320 330 340 350
QSSALCPSLA VVGSDAVDSG TCSSAFAGWE VHTRGIGSRL LTKMGYEFGK
360 370 380 390 400
GLGRHAEGRV EPIHAVVLPR GKSLDQCVET LQKQTRVGKA GTNKPPRCRG
410 420 430 440 450
RGARPGGRPA PRNVFDFLNE KLQGQAPGAL EAGAAPAGRR SKDMYHASKS
460 470 480 490 500
AKRALSLRLF QTEEKIERTQ RDIRSIQEAL ARNAGRHSVA SAQLQEKLAG
510 520 530
AQRQLGQLRA QEAGLQQEQR KADTHKKMTE F
Note: No experimental confirmation available.
Length:531
Mass (Da):57,359
Last modified:May 15, 2007 - v3
Checksum:i36572156BABDA876
GO
Isoform 2 (identifier: Q8N5A5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     291-310: Missing.

Show »
Length:511
Mass (Da):55,500
Checksum:i5007C362ECC2CD79
GO
Isoform 3 (identifier: Q8N5A5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     282-310: Missing.

Note: No experimental confirmation available.
Show »
Length:502
Mass (Da):54,605
Checksum:i8DD60FEEDB37D2F9
GO
Isoform 4 (identifier: Q8N5A5-4) [UniParc]FASTAAdd to basket
Also known as: sZIP

The sequence of this isoform differs from the canonical sequence as follows:
     1-343: Missing.

Show »
Length:188
Mass (Da):20,697
Checksum:i00ABC2D9BC65E7C9
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
X6RGY1X6RGY1_HUMAN
Zinc finger CCCH-type with G patch ...
ZGPAT
97Annotation score:

Sequence cautioni

The sequence BAB47476 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC11317 differs from that shown. Reason: Frameshift at position 290.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184L → Q in BAB55426 (PubMed:14702039).Curated1
Sequence conflicti188C → R in BAC11317 (PubMed:14702039).Curated1
Sequence conflicti344M → V in BAB55426 (PubMed:14702039).Curated1
Sequence conflicti433G → R in AAH19338 (PubMed:15489334).Curated1
Sequence conflicti519Q → R in BAB55426 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02553961S → R4 PublicationsCorresponds to variant dbSNP:rs1291212Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0535991 – 343Missing in isoform 4. CuratedAdd BLAST343
Alternative sequenceiVSP_038121282 – 310Missing in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_007754291 – 310Missing in isoform 2. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB058750 mRNA Translation: BAB47476.3 Different initiation.
AK027878 mRNA Translation: BAB55426.1
AK074961 mRNA Translation: BAC11317.1 Frameshift.
AL121845 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW75223.1
CH471077 Genomic DNA Translation: EAW75226.1
BC019338 mRNA Translation: AAH19338.1
BC032612 mRNA Translation: AAH32612.1
CCDSiCCDS13534.1 [Q8N5A5-1]
CCDS13535.1 [Q8N5A5-2]
CCDS56203.1 [Q8N5A5-3]
RefSeqiNP_001076582.1, NM_001083113.1 [Q8N5A5-2]
NP_001182582.1, NM_001195653.1 [Q8N5A5-2]
NP_001182583.1, NM_001195654.1 [Q8N5A5-3]
NP_115916.3, NM_032527.4 [Q8N5A5-1]
NP_852150.2, NM_181485.2 [Q8N5A5-2]
UniGeneiHs.590868

Genome annotation databases

EnsembliENST00000328969; ENSP00000332013; ENSG00000197114 [Q8N5A5-1]
ENST00000355969; ENSP00000348242; ENSG00000197114 [Q8N5A5-2]
ENST00000357119; ENSP00000349634; ENSG00000197114 [Q8N5A5-3]
ENST00000369967; ENSP00000358984; ENSG00000197114 [Q8N5A5-2]
ENST00000448100; ENSP00000391176; ENSG00000197114 [Q8N5A5-2]
GeneIDi84619
KEGGihsa:84619
UCSCiuc002ygi.3 human [Q8N5A5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiZGPAT_HUMAN
AccessioniPrimary (citable) accession number: Q8N5A5
Secondary accession number(s): E1P5K1
, Q4VXN9, Q5JWI9, Q5JWJ0, Q8NC55, Q8WUV4, Q96JI0, Q96JU4, Q9H401
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: May 15, 2007
Last modified: September 12, 2018
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health

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