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Protein

Serine/threonine-protein kinase DCLK2

Gene

DCLK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei423ATPPROSITE-ProRule annotation1
Active sitei515Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi400 – 408ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ8N568

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase DCLK2 (EC:2.7.11.1)
Alternative name(s):
CaMK-like CREB regulatory kinase 2
Short name:
CL2
Short name:
CLICK-II
Short name:
CLICK2
Doublecortin domain-containing protein 3B
Doublecortin-like and CAM kinase-like 2
Doublecortin-like kinase 2
Gene namesi
Name:DCLK2
Synonyms:DCAMKL2, DCDC3B, DCK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000170390.15
HGNCiHGNC:19002 DCLK2
MIMi613166 gene
neXtProtiNX_Q8N568

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000170390
PharmGKBiPA162383366

Chemistry databases

ChEMBLiCHEMBL5519
GuidetoPHARMACOLOGYi2006

Polymorphism and mutation databases

BioMutaiDCLK2
DMDMi296439470

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859221 – 766Serine/threonine-protein kinase DCLK2Add BLAST766

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61PhosphothreonineCombined sources1
Modified residuei362PhosphoserineCombined sources1
Modified residuei647PhosphoserineBy similarity1
Modified residuei666PhosphothreonineBy similarity1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8N568
PaxDbiQ8N568
PeptideAtlasiQ8N568
PRIDEiQ8N568
ProteomicsDBi72008
72009 [Q8N568-2]
72010 [Q8N568-3]

PTM databases

iPTMnetiQ8N568
PhosphoSitePlusiQ8N568

Expressioni

Tissue specificityi

Expressed in the brain, heart and eyes.1 Publication

Developmental stagei

Expressed in fetal brain, and, to a lower extent, in fetal kidney.1 Publication

Gene expression databases

BgeeiENSG00000170390 Expressed in 115 organ(s), highest expression level in oocyte
CleanExiHS_DCLK2
ExpressionAtlasiQ8N568 baseline and differential
GenevisibleiQ8N568 HS

Organism-specific databases

HPAiHPA015770

Interactioni

Subunit structurei

Binds to and stabilizes microtubules. Interacts with MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK9/JNK2, PPP1R9B/NEURABIN-2 and actin.By similarity

Protein-protein interaction databases

BioGridi127929, 7 interactors
IntActiQ8N568, 4 interactors
STRINGi9606.ENSP00000303887

Chemistry databases

BindingDBiQ8N568

Structurei

3D structure databases

ProteinModelPortaliQ8N568
SMRiQ8N568
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 158Doublecortin 1PROSITE-ProRule annotationAdd BLAST87
Domaini197 – 280Doublecortin 2PROSITE-ProRule annotationAdd BLAST84
Domaini394 – 651Protein kinasePROSITE-ProRule annotationAdd BLAST258

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi293 – 362Ser-richAdd BLAST70
Compositional biasi713 – 753Pro-richAdd BLAST41

Domaini

The doublecortin domains are involved in the colocalization with microtubules.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0615 Eukaryota
ENOG410YA63 LUCA
GeneTreeiENSGT00840000129744
HOGENOMiHOG000230855
HOVERGENiHBG003790
InParanoidiQ8N568
KOiK08805
OMAiERAGTWR
OrthoDBiEOG091G02RF
PhylomeDBiQ8N568
TreeFamiTF318770

Family and domain databases

CDDicd01617 DCX, 2 hits
Gene3Di3.10.20.230, 2 hits
InterProiView protein in InterPro
IPR003533 Doublecortin_dom
IPR036572 Doublecortin_dom_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF03607 DCX, 2 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00537 DCX, 2 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
SSF89837 SSF89837, 2 hits
PROSITEiView protein in PROSITE
PS50309 DC, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8N568-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASTRSIELE HFEERDKRPR PGSRRGAPSS SGGSSSSGPK GNGLIPSPAH
60 70 80 90 100
SAHCSFYRTR TLQALSSEKK AKKARFYRNG DRYFKGLVFA ISSDRFRSFD
110 120 130 140 150
ALLIELTRSL SDNVNLPQGV RTIYTIDGSR KVTSLDELLE GESYVCASNE
160 170 180 190 200
PFRKVDYTKN INPNWSVNIK GGTSRALAAA SSVKSEVKES KDFIKPKLVT
210 220 230 240 250
VIRSGVKPRK AVRILLNKKT AHSFEQVLTD ITEAIKLDSG VVKRLCTLDG
260 270 280 290 300
KQVTCLQDFF GDDDVFIACG PEKFRYAQDD FVLDHSECRV LKSSYSRSSA
310 320 330 340 350
VKYSGSKSPG PSRRSKSPAS VNGTPSSQLS TPKSTKSSSS SPTSPGSFRG
360 370 380 390 400
LKQISAHGRS SSNVNGGPEL DRCISPEGVN GNRCSESSTL LEKYKIGKVI
410 420 430 440 450
GDGNFAVVKE CIDRSTGKEF ALKIIDKAKC CGKEHLIENE VSILRRVKHP
460 470 480 490 500
NIIMLVEEME TATELFLVME LVKGGDLFDA ITSSTKYTER DGSAMVYNLA
510 520 530 540 550
NALRYLHGLS IVHRDIKPEN LLVCEYPDGT KSLKLGDFGL ATVVEGPLYT
560 570 580 590 600
VCGTPTYVAP EIIAETGYGL KVDIWAAGVI TYILLCGFPP FRSENNLQED
610 620 630 640 650
LFDQILAGKL EFPAPYWDNI TDSAKELISQ MLQVNVEARC TAGQILSHPW
660 670 680 690 700
VSDDASQENN MQAEVTGKLK QHFNNALPKQ NSTTTGVSVI MNTALDKEGQ
710 720 730 740 750
IFCSKHCQDS GRPGMEPISP VPPSVEEIPV PGEAVPAPTP PESPTPHPPP
760
AAPGGERAGT WRRHRD
Length:766
Mass (Da):83,606
Last modified:May 18, 2010 - v4
Checksum:i024F3223874AE83C
GO
Isoform 2 (identifier: Q8N568-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     353-353: Missing.

Note: By homology to mouse isoform 2.
Show »
Length:765
Mass (Da):83,478
Checksum:i48EE55037EFF8AD8
GO
Isoform 3 (identifier: Q8N568-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     321-321: V → VKRGGHYSSAYSTAKSPV

Show »
Length:783
Mass (Da):85,384
Checksum:iD69154961F0CFF77
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G5E9L9G5E9L9_HUMAN
Doublecortin and CaM kinase-like 2,...
DCLK2 DCAMKL2, hCG_18043
695Annotation score:
A0A0U1RR70A0A0U1RR70_HUMAN
Serine/threonine-protein kinase DCL...
DCLK2
716Annotation score:
A0A0U1RRD0A0A0U1RRD0_HUMAN
Serine/threonine-protein kinase DCL...
DCLK2
78Annotation score:

Sequence cautioni

The sequence BAD92418 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti694A → V in CAD39156 (PubMed:17974005).Curated1
Sequence conflicti748P → C in BAD92418 (Ref. 1) Curated1
Sequence conflicti748P → C in CAD39156 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_040441119G → C1 PublicationCorresponds to variant dbSNP:rs56327537Ensembl.1
Natural variantiVAR_073158212V → M Found in a patient with hereditary motor and sensory neuropathy; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs759398144Ensembl.1
Natural variantiVAR_040442372R → H1 PublicationCorresponds to variant dbSNP:rs34386880Ensembl.1
Natural variantiVAR_040443583I → V1 PublicationCorresponds to variant dbSNP:rs35745104Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012793321V → VKRGGHYSSAYSTAKSPV in isoform 3. 1 Publication1
Alternative sequenceiVSP_012794353Missing in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB209181 mRNA Translation: BAD92418.1 Different initiation.
AC093748 Genomic DNA No translation available.
AC105343 Genomic DNA No translation available.
AC108934 Genomic DNA No translation available.
AC118064 Genomic DNA No translation available.
AL834498 mRNA Translation: CAD39156.1
CCDSiCCDS34076.1 [Q8N568-1]
CCDS47142.2 [Q8N568-3]
RefSeqiNP_001035350.2, NM_001040260.3 [Q8N568-1]
NP_001035351.4, NM_001040261.4 [Q8N568-3]
XP_005262843.1, XM_005262786.2 [Q8N568-2]
UniGeneiHs.591683

Genome annotation databases

EnsembliENST00000296550; ENSP00000296550; ENSG00000170390 [Q8N568-1]
ENST00000302176; ENSP00000303887; ENSG00000170390 [Q8N568-3]
ENST00000506325; ENSP00000427235; ENSG00000170390 [Q8N568-2]
GeneIDi166614
KEGGihsa:166614
UCSCiuc003ilm.5 human [Q8N568-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB209181 mRNA Translation: BAD92418.1 Different initiation.
AC093748 Genomic DNA No translation available.
AC105343 Genomic DNA No translation available.
AC108934 Genomic DNA No translation available.
AC118064 Genomic DNA No translation available.
AL834498 mRNA Translation: CAD39156.1
CCDSiCCDS34076.1 [Q8N568-1]
CCDS47142.2 [Q8N568-3]
RefSeqiNP_001035350.2, NM_001040260.3 [Q8N568-1]
NP_001035351.4, NM_001040261.4 [Q8N568-3]
XP_005262843.1, XM_005262786.2 [Q8N568-2]
UniGeneiHs.591683

3D structure databases

ProteinModelPortaliQ8N568
SMRiQ8N568
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127929, 7 interactors
IntActiQ8N568, 4 interactors
STRINGi9606.ENSP00000303887

Chemistry databases

BindingDBiQ8N568
ChEMBLiCHEMBL5519
GuidetoPHARMACOLOGYi2006

PTM databases

iPTMnetiQ8N568
PhosphoSitePlusiQ8N568

Polymorphism and mutation databases

BioMutaiDCLK2
DMDMi296439470

Proteomic databases

MaxQBiQ8N568
PaxDbiQ8N568
PeptideAtlasiQ8N568
PRIDEiQ8N568
ProteomicsDBi72008
72009 [Q8N568-2]
72010 [Q8N568-3]

Protocols and materials databases

DNASUi166614
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296550; ENSP00000296550; ENSG00000170390 [Q8N568-1]
ENST00000302176; ENSP00000303887; ENSG00000170390 [Q8N568-3]
ENST00000506325; ENSP00000427235; ENSG00000170390 [Q8N568-2]
GeneIDi166614
KEGGihsa:166614
UCSCiuc003ilm.5 human [Q8N568-1]

Organism-specific databases

CTDi166614
EuPathDBiHostDB:ENSG00000170390.15
GeneCardsiDCLK2
HGNCiHGNC:19002 DCLK2
HPAiHPA015770
MIMi613166 gene
neXtProtiNX_Q8N568
OpenTargetsiENSG00000170390
PharmGKBiPA162383366
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0615 Eukaryota
ENOG410YA63 LUCA
GeneTreeiENSGT00840000129744
HOGENOMiHOG000230855
HOVERGENiHBG003790
InParanoidiQ8N568
KOiK08805
OMAiERAGTWR
OrthoDBiEOG091G02RF
PhylomeDBiQ8N568
TreeFamiTF318770

Enzyme and pathway databases

SignaLinkiQ8N568

Miscellaneous databases

ChiTaRSiDCLK2 human
GenomeRNAii166614
PROiPR:Q8N568
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170390 Expressed in 115 organ(s), highest expression level in oocyte
CleanExiHS_DCLK2
ExpressionAtlasiQ8N568 baseline and differential
GenevisibleiQ8N568 HS

Family and domain databases

CDDicd01617 DCX, 2 hits
Gene3Di3.10.20.230, 2 hits
InterProiView protein in InterPro
IPR003533 Doublecortin_dom
IPR036572 Doublecortin_dom_sf
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF03607 DCX, 2 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00537 DCX, 2 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
SSF89837 SSF89837, 2 hits
PROSITEiView protein in PROSITE
PS50309 DC, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDCLK2_HUMAN
AccessioniPrimary (citable) accession number: Q8N568
Secondary accession number(s): C9J5Q9, Q59GC8, Q8N399
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 18, 2010
Last modified: September 12, 2018
This is version 145 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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