Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Prolyl 3-hydroxylase OGFOD1

Gene

OGFOD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Prolyl 3-hydroxylase that catalyzes 3-hydroxylation of 'Pro-62' of small ribosomal subunit uS12 (RPS23), thereby regulating protein translation termination efficiency. Involved in stress granule formation.3 Publications

Caution

According to a report, it is incorporated into stress granules upon arsenite-induced stress and associates with heme-regulated kinase and eIF-2-alpha (EIF2S1) in regulating eIF-2-alpha phosphorylation (PubMed:20154146). However, no effect in eIF-2-alpha phosphorylation have been observed by another study (PubMed:24550447).2 Publications

Catalytic activityi

[ribosomal protein uS12]-L-proline + 2-oxoglutarate + O2 = [ribosomal protein uS12]-(3S)-3-hydroxy-L-proline + succinate + CO2.1 Publication1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Fe2+PROSITE-ProRule annotationNote: Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi155Iron; via tele nitrogenPROSITE-ProRule annotation1 Publication1
Metal bindingi157IronPROSITE-ProRule annotation1 Publication1
Binding sitei1692-oxoglutarate1 Publication1
Metal bindingi218Iron; via tele nitrogenPROSITE-ProRule annotation1 Publication1
Binding sitei2302-oxoglutaratePROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
LigandIron, Metal-binding, Vitamin C

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase OGFOD1 (EC:1.14.11.-)
Alternative name(s):
2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1
Termination and polyadenylation 1 homolog
uS12 prolyl 3-hydroxylase1 Publication
Gene namesi
Name:OGFOD1
Synonyms:KIAA1612, TPA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000087263.16
HGNCiHGNC:25585 OGFOD1
MIMi615857 gene
neXtProtiNX_Q8N543

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi155H → A: Loss of function. 1 Publication1
Mutagenesisi157D → A: Loss of function. 1 Publication1

Organism-specific databases

DisGeNETi55239
OpenTargetsiENSG00000087263
PharmGKBiPA143485568

Chemistry databases

DrugBankiDB00126 Vitamin C

Polymorphism and mutation databases

BioMutaiOGFOD1
DMDMi74728942

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002889741 – 542Prolyl 3-hydroxylase OGFOD1Add BLAST542

Proteomic databases

EPDiQ8N543
MaxQBiQ8N543
PaxDbiQ8N543
PeptideAtlasiQ8N543
PRIDEiQ8N543
ProteomicsDBi72000
72001 [Q8N543-2]

PTM databases

iPTMnetiQ8N543
PhosphoSitePlusiQ8N543

Expressioni

Gene expression databases

BgeeiENSG00000087263 Expressed in 219 organ(s), highest expression level in adrenal tissue
CleanExiHS_OGFOD1
ExpressionAtlasiQ8N543 baseline and differential
GenevisibleiQ8N543 HS

Organism-specific databases

HPAiHPA003215

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi120532, 34 interactors
IntActiQ8N543, 1 interactor
STRINGi9606.ENSP00000457258

Structurei

Secondary structure

1542
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8N543
SMRiQ8N543
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini134 – 239Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST106

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi4 – 20Nuclear localization signalCuratedAdd BLAST17

Sequence similaritiesi

Belongs to the TPA1 family.Curated

Phylogenomic databases

eggNOGiKOG3844 Eukaryota
COG3751 LUCA
GeneTreeiENSGT00390000002349
HOGENOMiHOG000007015
HOVERGENiHBG056995
InParanoidiQ8N543
OMAiFSFVYYE
OrthoDBiEOG091G08PF
PhylomeDBiQ8N543
TreeFamiTF105920

Family and domain databases

InterProiView protein in InterPro
IPR005123 Oxoglu/Fe-dep_dioxygenase
IPR019601 Oxoglutarate/Fe-dep_Oase_C
IPR006620 Pro_4_hyd_alph
PfamiView protein in Pfam
PF10637 Ofd1_CTDD, 1 hit
SMARTiView protein in SMART
SM00702 P4Hc, 1 hit
PROSITEiView protein in PROSITE
PS51471 FE2OG_OXY, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q8N543-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNGKRPAEPG PARVGKKGKK EVMAEFSDAV TEETLKKQVA EAWSRRTPFS
60 70 80 90 100
HEVIVMDMDP FLHCVIPNFI QSQDFLEGLQ KELMNLDFHE KYNDLYKFQQ
110 120 130 140 150
SDDLKKRREP HISTLRKILF EDFRSWLSDI SKIDLESTID MSCAKYEFTD
160 170 180 190 200
ALLCHDDELE GRRIAFILYL VPPWDRSMGG TLDLYSIDEH FQPKQIVKSL
210 220 230 240 250
IPSWNKLVFF EVSPVSFHQV SEVLSEEKSR LSISGWFHGP SLTRPPNYFE
260 270 280 290 300
PPIPRSPHIP QDHEILYDWI NPTYLDMDYQ VQIQEEFEES SEILLKEFLK
310 320 330 340 350
PEKFTKVCEA LEHGHVEWSS RGPPNKRFYE KAEESKLPEI LKECMKLFRS
360 370 380 390 400
EALFLLLSNF TGLKLHFLAP SEEDEMNDKK EAETTDITEE GTSHSPPEPE
410 420 430 440 450
NNQMAISNNS QQSNEQTDPE PEENETKKES SVPMCQGELR HWKTGHYTLI
460 470 480 490 500
HDHSKAEFAL DLILYCGCEG WEPEYGGFTS YIAKGEDEEL LTVNPESNSL
510 520 530 540
ALVYRDRETL KFVKHINHRS LEQKKTFPNR TGFWDFSFIY YE
Length:542
Mass (Da):63,246
Last modified:October 1, 2002 - v1
Checksum:i8834BB200D5D8D85
GO
Isoform 2 (identifier: Q8N543-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-140: Missing.

Note: No experimental confirmation available.
Show »
Length:402
Mass (Da):46,861
Checksum:i8309970C9ED3AEB8
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MTR2A0A0A0MTR2_HUMAN
Prolyl 3-hydroxylase OGFOD1
OGFOD1
499Annotation score:
H3BUA6H3BUA6_HUMAN
Prolyl 3-hydroxylase OGFOD1
OGFOD1
175Annotation score:
H3BP48H3BP48_HUMAN
Prolyl 3-hydroxylase OGFOD1
OGFOD1
46Annotation score:
H3BR02H3BR02_HUMAN
Prolyl 3-hydroxylase OGFOD1
OGFOD1
109Annotation score:
H3BR79H3BR79_HUMAN
Prolyl 3-hydroxylase OGFOD1
OGFOD1
164Annotation score:
J3KNR5J3KNR5_HUMAN
Prolyl 3-hydroxylase OGFOD1
OGFOD1
39Annotation score:

Sequence cautioni

The sequence BAB13438 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB14880 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50S → G in BAB13967 (PubMed:14702039).Curated1
Sequence conflicti112I → V in BAB14226 (PubMed:14702039).Curated1
Sequence conflicti343E → G in BAB14880 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_032545173P → S1 PublicationCorresponds to variant dbSNP:rs34883368Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0258521 – 140Missing in isoform 2. 1 PublicationAdd BLAST140

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046832 mRNA Translation: BAB13438.1 Different initiation.
AK001688 mRNA Translation: BAA91838.1
AK022130 mRNA Translation: BAB13967.1
AK022752 mRNA Translation: BAB14226.1
AK024314 mRNA Translation: BAB14880.1 Different initiation.
AC092140 Genomic DNA No translation available.
BC032919 mRNA Translation: AAH32919.1
CCDSiCCDS10761.2 [Q8N543-1]
RefSeqiNP_001311291.1, NM_001324362.1 [Q8N543-2]
NP_060703.3, NM_018233.3
UniGeneiHs.231883

Genome annotation databases

EnsembliENST00000566157; ENSP00000457258; ENSG00000087263 [Q8N543-1]
GeneIDi55239
KEGGihsa:55239
UCSCiuc002ejb.4 human [Q8N543-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Entry informationi

Entry nameiOGFD1_HUMAN
AccessioniPrimary (citable) accession number: Q8N543
Secondary accession number(s): H3BUQ2
, Q9H7U5, Q9H9J9, Q9HA87, Q9HCG0, Q9NVB6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2002
Last modified: September 12, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again