UniProtKB - Q8N4V1 (EMC5_HUMAN)
Protein
ER membrane protein complex subunit 5
Gene
MMGT1
Organism
Homo sapiens (Human)
Status
Functioni
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (By similarity). May be involved in Mg2+ transport (By similarity).By similarity5 Publications
GO - Molecular functioni
- cobalt ion transmembrane transporter activity Source: Ensembl
- ferrous iron transmembrane transporter activity Source: Ensembl
- inorganic cation transmembrane transporter activity Source: GO_Central
- magnesium ion transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- copper ion transport Source: Ensembl
- magnesium ion transport Source: UniProtKB
Keywordsi
Biological process | Transport |
Ligand | Magnesium |
Enzyme and pathway databases
PathwayCommonsi | Q8N4V1 |
Reactomei | R-HSA-5223345, Miscellaneous transport and binding events |
Protein family/group databases
TCDBi | 3.A.27.1.1, the endoplasmic reticulum membrane protein insertion complex (emc) family |
Names & Taxonomyi
Protein namesi | Recommended name: ER membrane protein complex subunit 51 PublicationAlternative name(s): Membrane magnesium transporter 1Imported Transmembrane protein 32Imported |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28100, MMGT1 |
neXtProti | NX_Q8N4V1 |
VEuPathDBi | HostDB:ENSG00000169446.5 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein 2 Publications
Endosome
- Early endosome membrane By similarity; Multi-pass membrane protein 2 Publications
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 2 Publications
Endoplasmic reticulum
- ER membrane protein complex Source: UniProtKB
Endosome
- early endosome Source: UniProtKB
- early endosome membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 3 | Cytoplasmic1 Publication | 3 | |
Transmembranei | 4 – 22 | Helical1 PublicationAdd BLAST | 19 | |
Topological domaini | 23 – 43 | Lumenal1 PublicationAdd BLAST | 21 | |
Transmembranei | 44 – 63 | Helical1 PublicationAdd BLAST | 20 | |
Topological domaini | 64 – 131 | Cytoplasmic1 PublicationAdd BLAST | 68 |
Keywords - Cellular componenti
Endoplasmic reticulum, Endosome, Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000169446 |
PharmGKBi | PA164723074 |
Miscellaneous databases
Pharosi | Q8N4V1, Tdark |
Genetic variation databases
BioMutai | MMGT1 |
DMDMi | 74751006 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000286435 | 1 – 131 | ER membrane protein complex subunit 5Add BLAST | 131 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 120 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8N4V1 |
jPOSTi | Q8N4V1 |
MassIVEi | Q8N4V1 |
MaxQBi | Q8N4V1 |
PaxDbi | Q8N4V1 |
PeptideAtlasi | Q8N4V1 |
PRIDEi | Q8N4V1 |
ProteomicsDBi | 71978 [Q8N4V1-1] 71979 [Q8N4V1-2] |
TopDownProteomicsi | Q8N4V1-1 [Q8N4V1-1] |
PTM databases
iPTMneti | Q8N4V1 |
PhosphoSitePlusi | Q8N4V1 |
SwissPalmi | Q8N4V1 |
Expressioni
Gene expression databases
Bgeei | ENSG00000169446, Expressed in forebrain and 221 other tissues |
Genevisiblei | Q8N4V1, HS |
Organism-specific databases
HPAi | ENSG00000169446, Low tissue specificity |
Interactioni
Subunit structurei
Component of the ER membrane protein complex (EMC).
4 PublicationsBinary interactionsi
Q8N4V1
Protein-protein interaction databases
BioGRIDi | 125021, 325 interactors |
ComplexPortali | CPX-5848, Endoplasmic reticulum membrane complex, ECM8 variant CPX-5881, Endoplasmic reticulum membrane complex, ECM9 variant |
IntActi | Q8N4V1, 162 interactors |
STRINGi | 9606.ENSP00000306220 |
Miscellaneous databases
RNActi | Q8N4V1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8N4V1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3918, Eukaryota |
GeneTreei | ENSGT00510000047104 |
HOGENOMi | CLU_122437_1_0_1 |
InParanoidi | Q8N4V1 |
OMAi | WRSEMKR |
OrthoDBi | 1558871at2759 |
PhylomeDBi | Q8N4V1 |
TreeFami | TF323267 |
Family and domain databases
InterProi | View protein in InterPro IPR018937, Magnesium_transport |
Pfami | View protein in Pfam PF10270, MMgT, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8N4V1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPSLWKGLV GIGLFALAHA AFSAAQHRSY MRLTEKEDES LPIDIVLQTL
60 70 80 90 100
LAFAVTCYGI VHIAGEFKDM DATSELKNKT FDTLRNHPSF YVFNHRGRVL
110 120 130
FRPSDTANSS NQDALSSNTS LKLRKLESLR R
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036488 | 1 | M → MTPLGSGPPREASIAQPSGF STTETLCAQDFSDVIFLRRA DTRRWKKKQLRRPSLLLLGC CSFGIM in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK295829 mRNA Translation: BAG58645.1 AK312409 mRNA Translation: BAG35322.1 AL732579 Genomic DNA No translation available. AL953870 Genomic DNA No translation available. CH471150 Genomic DNA Translation: EAW88483.1 CH471150 Genomic DNA Translation: EAW88485.1 BC033588 mRNA Translation: AAH33588.1 |
CCDSi | CCDS14653.1 [Q8N4V1-1] |
RefSeqi | NP_001316929.1, NM_001330000.1 [Q8N4V1-1] NP_775741.1, NM_173470.2 [Q8N4V1-1] |
Genome annotation databases
Ensembli | ENST00000305963; ENSP00000306220; ENSG00000169446 [Q8N4V1-1] |
GeneIDi | 93380 |
KEGGi | hsa:93380 |
UCSCi | uc004ezi.2, human [Q8N4V1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK295829 mRNA Translation: BAG58645.1 AK312409 mRNA Translation: BAG35322.1 AL732579 Genomic DNA No translation available. AL953870 Genomic DNA No translation available. CH471150 Genomic DNA Translation: EAW88483.1 CH471150 Genomic DNA Translation: EAW88485.1 BC033588 mRNA Translation: AAH33588.1 |
CCDSi | CCDS14653.1 [Q8N4V1-1] |
RefSeqi | NP_001316929.1, NM_001330000.1 [Q8N4V1-1] NP_775741.1, NM_173470.2 [Q8N4V1-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6WW7 | electron microscopy | 3.40 | E | 1-131 | [»] | |
6Z3W | electron microscopy | 6.40 | E | 1-131 | [»] | |
SMRi | Q8N4V1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 125021, 325 interactors |
ComplexPortali | CPX-5848, Endoplasmic reticulum membrane complex, ECM8 variant CPX-5881, Endoplasmic reticulum membrane complex, ECM9 variant |
IntActi | Q8N4V1, 162 interactors |
STRINGi | 9606.ENSP00000306220 |
Protein family/group databases
TCDBi | 3.A.27.1.1, the endoplasmic reticulum membrane protein insertion complex (emc) family |
PTM databases
iPTMneti | Q8N4V1 |
PhosphoSitePlusi | Q8N4V1 |
SwissPalmi | Q8N4V1 |
Genetic variation databases
BioMutai | MMGT1 |
DMDMi | 74751006 |
Proteomic databases
EPDi | Q8N4V1 |
jPOSTi | Q8N4V1 |
MassIVEi | Q8N4V1 |
MaxQBi | Q8N4V1 |
PaxDbi | Q8N4V1 |
PeptideAtlasi | Q8N4V1 |
PRIDEi | Q8N4V1 |
ProteomicsDBi | 71978 [Q8N4V1-1] 71979 [Q8N4V1-2] |
TopDownProteomicsi | Q8N4V1-1 [Q8N4V1-1] |
Protocols and materials databases
Antibodypediai | 51536, 109 antibodies |
Genome annotation databases
Ensembli | ENST00000305963; ENSP00000306220; ENSG00000169446 [Q8N4V1-1] |
GeneIDi | 93380 |
KEGGi | hsa:93380 |
UCSCi | uc004ezi.2, human [Q8N4V1-1] |
Organism-specific databases
CTDi | 93380 |
GeneCardsi | MMGT1 |
HGNCi | HGNC:28100, MMGT1 |
HPAi | ENSG00000169446, Low tissue specificity |
neXtProti | NX_Q8N4V1 |
OpenTargetsi | ENSG00000169446 |
PharmGKBi | PA164723074 |
VEuPathDBi | HostDB:ENSG00000169446.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3918, Eukaryota |
GeneTreei | ENSGT00510000047104 |
HOGENOMi | CLU_122437_1_0_1 |
InParanoidi | Q8N4V1 |
OMAi | WRSEMKR |
OrthoDBi | 1558871at2759 |
PhylomeDBi | Q8N4V1 |
TreeFami | TF323267 |
Enzyme and pathway databases
PathwayCommonsi | Q8N4V1 |
Reactomei | R-HSA-5223345, Miscellaneous transport and binding events |
Miscellaneous databases
BioGRID-ORCSi | 93380, 127 hits in 512 CRISPR screens |
ChiTaRSi | MMGT1, human |
GenomeRNAii | 93380 |
Pharosi | Q8N4V1, Tdark |
PROi | PR:Q8N4V1 |
RNActi | Q8N4V1, protein |
Gene expression databases
Bgeei | ENSG00000169446, Expressed in forebrain and 221 other tissues |
Genevisiblei | Q8N4V1, HS |
Family and domain databases
InterProi | View protein in InterPro IPR018937, Magnesium_transport |
Pfami | View protein in Pfam PF10270, MMgT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EMC5_HUMAN | |
Accessioni | Q8N4V1Primary (citable) accession number: Q8N4V1 Secondary accession number(s): B2R625 Q5JPP7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families