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Protein

Carnosine N-methyltransferase

Gene

CARNMT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

N-methyltransferase that mediates the formation of anserine (beta-alanyl-N(Pi)-methyl-L-histidine) from carnosine. Anserine, a methylated derivative of carnosine (beta-alanyl-L-histidine), is an abundant constituent of vertebrate skeletal muscles. Also methylates other L-histidine-containing di- and tripeptides such as Gly-Gly-His, Gly-His and homocarnosine (GABA-His).1 Publication

Catalytic activityi

S-adenosyl-L-methionine + carnosine = S-adenosyl-L-homocysteine + anserine.1 Publication

Kineticsi

kcat is 0.09 min(-1) for carnosine. kcat is 3.57 min(-1) for S-adenosyl-L-methionine.1 Publication
  1. KM=4.959 mM for carnosine1 Publication
  2. KM=0.053 mM for S-adenosyl-L-methionine1 Publication
  1. Vmax=1.77 nmol/min/mg enzyme with carnosine as substrate1 Publication

pH dependencei

Optimum pH is 7.0-7.5.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius.1 Publication

GO - Molecular functioni

  • carnosine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • carnosine metabolic process Source: UniProtKB
  • histidine catabolic process Source: Reactome

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-HSA-70921 Histidine catabolism
SABIO-RKiQ8N4J0

Names & Taxonomyi

Protein namesi
Recommended name:
Carnosine N-methyltransferase1 PublicationImported (EC:2.1.1.221 Publication)
Gene namesi
Name:CARNMT1Imported
Synonyms:C9orf41Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000156017.12
HGNCiHGNC:23435 CARNMT1
MIMi616552 gene
neXtProtiNX_Q8N4J0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000156017
PharmGKBiPA134929393

Polymorphism and mutation databases

BioMutaiC9orf41
DMDMi68565210

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000896851 – 409Carnosine N-methyltransferaseAdd BLAST409

Proteomic databases

EPDiQ8N4J0
MaxQBiQ8N4J0
PaxDbiQ8N4J0
PeptideAtlasiQ8N4J0
PRIDEiQ8N4J0
ProteomicsDBi71936

PTM databases

iPTMnetiQ8N4J0
PhosphoSitePlusiQ8N4J0

Expressioni

Tissue specificityi

Expressed at higher level in kidney. Expressed at lower level in brain and skeletal musclea.1 Publication

Gene expression databases

BgeeiENSG00000156017
CleanExiHS_C9orf41
ExpressionAtlasiQ8N4J0 baseline and differential
GenevisibleiQ8N4J0 HS

Organism-specific databases

HPAiHPA026756

Interactioni

Protein-protein interaction databases

BioGridi126504, 44 interactors
IntActiQ8N4J0, 3 interactors
STRINGi9606.ENSP00000366030

Structurei

3D structure databases

ProteinModelPortaliQ8N4J0
SMRiQ8N4J0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2798 Eukaryota
ENOG410Z0B7 LUCA
GeneTreeiENSGT00390000005323
HOGENOMiHOG000204444
HOVERGENiHBG055166
InParanoidiQ8N4J0
KOiK19787
OMAiADFLCLY
OrthoDBiEOG091G0CID
PhylomeDBiQ8N4J0
TreeFamiTF313564

Family and domain databases

InterProiView protein in InterPro
IPR012901 N2227
IPR029063 SAM-dependent_MTases
PANTHERiPTHR12303 PTHR12303, 1 hit
PfamiView protein in Pfam
PF07942 N2227, 1 hit
SMARTiView protein in SMART
SM01296 N2227, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Q8N4J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRRRRPPPP TSRLPEGCGG GGGGSEEVEV QFSAGRWGSA AAVSAAAAAA
60 70 80 90 100
TRSTEEEEER LEREHFWKII NAFRYYGTSM HERVNRTERQ FRSLPANQQK
110 120 130 140 150
LLPQFLLHLD KIRKCIDHNQ EILLTIVNDC IHMFENKEYG EDGNGKIMPA
160 170 180 190 200
STFDMDKLKS TLKQFVRDWS ETGKAERDAC YQPIIKEILK NFPKERWDPS
210 220 230 240 250
KVNILVPGAG LGRLAWEIAM LGYACQGNEW SFFMLFSSNF VLNRCSEINK
260 270 280 290 300
YKLYPWIHQF SNNRRSADQI RPIFFPDVDP HSLPPGSNFS MTAGDFQEIY
310 320 330 340 350
SECNTWDCIA TCFFIDTAHN VIDYIDTIWK ILKPGGIWIN LGPLLYHFEN
360 370 380 390 400
LANELSIELS YEDIKNVVLQ YGFKVEVEKE SVLSTYTVND LSMMKYYYEC

VLFVVRKPQ
Length:409
Mass (Da):47,186
Last modified:October 1, 2002 - v1
Checksum:i458584B41E1794EF
GO

Sequence cautioni

The sequence BAC05369 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti333K → R in CAD97992 (PubMed:17974005).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL158825 Genomic DNA No translation available.
BC034033 mRNA Translation: AAH34033.1
BX538061 mRNA Translation: CAD97992.1
AK098661 mRNA Translation: BAC05369.1 Different initiation.
CCDSiCCDS6649.1
RefSeqiNP_001307426.1, NM_001320497.1
NP_689633.1, NM_152420.2
UniGeneiHs.567688

Genome annotation databases

EnsembliENST00000376834; ENSP00000366030; ENSG00000156017
GeneIDi138199
KEGGihsa:138199
UCSCiuc004ajq.4 human

Similar proteinsi

Entry informationi

Entry nameiCARME_HUMAN
AccessioniPrimary (citable) accession number: Q8N4J0
Secondary accession number(s): Q7Z383, Q8N7C5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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