UniProtKB - Q8N4A0 (GALT4_HUMAN)
Protein
Polypeptide N-acetylgalactosaminyltransferase 4
Gene
GALNT4
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a highest activity toward Muc7, EA2 and Muc2, with a lowest activity than GALNT2. Glycosylates 'Thr-57' of SELPLG.3 Publications
Catalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDP3 PublicationsEC:2.4.1.413 Publications
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDP3 PublicationsEC:2.4.1.413 Publications
Cofactori
Mn2+1 Publication
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.3 PublicationsView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 175 | SubstrateBy similarity | 1 | |
Binding sitei | 204 | SubstrateBy similarity | 1 | |
Metal bindingi | 227 | ManganeseBy similarity | 1 | |
Metal bindingi | 229 | ManganeseBy similarity | 1 | |
Binding sitei | 334 | SubstrateBy similarity | 1 | |
Metal bindingi | 362 | ManganeseBy similarity | 1 | |
Binding sitei | 370 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- manganese ion binding Source: UniProtKB
- polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB
GO - Biological processi
- O-glycan processing Source: Reactome
- protein O-linked glycosylation via serine Source: UniProtKB
- protein O-linked glycosylation via threonine Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.4.1.41, 2681 |
PathwayCommonsi | Q8N4A0 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: Polypeptide N-acetylgalactosaminyltransferase 4 (EC:2.4.1.413 Publications)Alternative name(s): Polypeptide GalNAc transferase 4 Short name: GalNAc-T41 Publication Short name: pp-GaNTase 4 Protein-UDP acetylgalactosaminyltransferase 4 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4 |
Gene namesi | Name:GALNT4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4126, GALNT4 |
MIMi | 603565, gene |
neXtProti | NX_Q8N4A0 |
VEuPathDBi | HostDB:ENSG00000257594.3 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 12 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 13 – 35 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 36 – 578 | LumenalSequence analysisAdd BLAST | 543 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 459 | D → H: Affects the glycopeptide specificity and abolishes ability to glycosylate Muc1, Muc2 and Muc5AC. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 100528030 8693 |
OpenTargetsi | ENSG00000257594 |
PharmGKBi | PA28539 |
Miscellaneous databases
Pharosi | Q8N4A0, Tbio |
Genetic variation databases
BioMutai | GALNT4 |
DMDMi | 338817878 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059108 | 1 – 578 | Polypeptide N-acetylgalactosaminyltransferase 4Add BLAST | 578 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 124 ↔ 357 | PROSITE-ProRule annotationCombined sources1 Publication | ||
Disulfide bondi | 348 ↔ 421 | PROSITE-ProRule annotationCombined sources1 Publication | ||
Disulfide bondi | 457 ↔ 477 | PROSITE-ProRule annotationCombined sources1 Publication | ||
Glycosylationi | 471 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 503 ↔ 518 | PROSITE-ProRule annotationCombined sources1 Publication | ||
Disulfide bondi | 547 ↔ 565 | PROSITE-ProRule annotationCombined sources1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q8N4A0 |
jPOSTi | Q8N4A0 |
MassIVEi | Q8N4A0 |
MaxQBi | Q8N4A0 |
PaxDbi | Q8N4A0 |
PeptideAtlasi | Q8N4A0 |
PRIDEi | Q8N4A0 |
ProteomicsDBi | 4651 71900 [Q8N4A0-1] |
PTM databases
GlyGeni | Q8N4A0, 2 sites, 1 O-linked glycan (1 site) |
iPTMneti | Q8N4A0 |
PhosphoSitePlusi | Q8N4A0 |
SwissPalmi | Q8N4A0 |
Expressioni
Tissue specificityi
Ubiquitous. Highly expressed in mucous cells.1 Publication
Gene expression databases
Bgeei | ENSG00000257594, Expressed in endometrium and 119 other tissues |
Genevisiblei | Q8N4A0, HS |
Organism-specific databases
HPAi | ENSG00000257594, Low tissue specificity |
Interactioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 459 | Interaction with glycopeptide substrate1 Publication | 1 | |
Sitei | 478 | Interaction with glycopeptide substrate1 Publication | 1 | |
Sitei | 483 | Interaction with glycopeptide substrate1 Publication | 1 |
Binary interactionsi
Q8N4A0
With | #Exp. | IntAct |
---|---|---|
HTT [P42858] | 12 | EBI-21555925,EBI-466029 |
Protein-protein interaction databases
BioGRIDi | 114240, 9 interactors 1529357, 1 interactor |
IntActi | Q8N4A0, 9 interactors |
STRINGi | 9606.ENSP00000436604 |
Miscellaneous databases
RNActi | Q8N4A0, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8N4A0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 444 – 577 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 134 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 134 – 243 | Catalytic subdomain AAdd BLAST | 110 | |
Regioni | 303 – 365 | Catalytic subdomain BAdd BLAST | 63 |
Domaini
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.2 Publications
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000163607 |
HOGENOMi | CLU_013477_0_1_1 |
InParanoidi | Q8N4A0 |
OMAi | MQTGEPM |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q8N4A0 |
TreeFami | TF352660 |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8N4A0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAVRWTWAGK SCLLLAFLTV AYIFVELLVS TFHASAGAGR ARELGSRRLS
60 70 80 90 100
DLQKNTEDLS RPLYKKPPAD SRALGEWGKA SKLQLNEDEL KQQEELIERY
110 120 130 140 150
AINIYLSDRI SLHRHIEDKR MYECKSQKFN YRTLPTTSVI IAFYNEAWST
160 170 180 190 200
LLRTIHSVLE TSPAVLLKEI ILVDDLSDRV YLKTQLETYI SNLDRVRLIR
210 220 230 240 250
TNKREGLVRA RLIGATFATG DVLTFLDCHC ECNSGWLEPL LERIGRDETA
260 270 280 290 300
VVCPVIDTID WNTFEFYMQI GEPMIGGFDW RLTFQWHSVP KQERDRRISR
310 320 330 340 350
IDPIRSPTMA GGLFAVSKKY FQYLGTYDTG MEVWGGENLE LSFRVWQCGG
360 370 380 390 400
KLEIHPCSHV GHVFPKRAPY ARPNFLQNTA RAAEVWMDEY KEHFYNRNPP
410 420 430 440 450
ARKEAYGDIS ERKLLRERLR CKSFDWYLKN VFPNLHVPED RPGWHGAIRS
460 470 480 490 500
RGISSECLDY NSPDNNPTGA NLSLFGCHGQ GGNQFFEYTS NKEIRFNSVT
510 520 530 540 550
ELCAEVPEQK NYVGMQNCPK DGFPVPANII WHFKEDGTIF HPHSGLCLSA
560 570
YRTPEGRPDV QMRTCDALDK NQIWSFEK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 11 | S → T in CAA69875 (PubMed:9804815).Curated | 1 | |
Sequence conflicti | 227 | D → Y in CAA69875 (PubMed:9804815).Curated | 1 | |
Sequence conflicti | 247 | D → Y in CAA69875 (PubMed:9804815).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_065257 | 51 | D → G1 PublicationCorresponds to variant dbSNP:rs17853610Ensembl. | 1 | |
Natural variantiVAR_019576 | 270 | I → T1 PublicationCorresponds to variant dbSNP:rs2230281Ensembl. | 1 | |
Natural variantiVAR_019577 | 506 | V → I2 PublicationsCorresponds to variant dbSNP:rs2230283Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045009 | 1 – 120 | MAVRW…IEDKR → MAWCVATADPAHTSRPLFTG LAVSRGSAGHAWSAGFDWAA VVVVTGRRCRSGQTVPGAAR SPLLPHPLPSPLRVPPPTGA LGRPLPRWPQPRRTPFWSVI SKATKLRSPPWTSAPTASNL in isoform 2. 1 PublicationAdd BLAST | 120 | |
Alternative sequenceiVSP_045010 | 121 – 292 | Missing in isoform 2. 1 PublicationAdd BLAST | 172 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y08564 Genomic DNA Translation: CAA69875.1 AK297677 mRNA Translation: BAG60038.1 AK312870 mRNA Translation: BAG35722.1 AC010201 Genomic DNA No translation available. AC025034 Genomic DNA No translation available. BC036390 mRNA Translation: AAH36390.1 |
CCDSi | CCDS53817.1 [Q8N4A0-1] |
RefSeqi | NP_001186711.1, NM_001199782.1 [Q8N4A0-2] NP_003765.2, NM_003774.4 [Q8N4A0-1] |
Genome annotation databases
Ensembli | ENST00000529983; ENSP00000436604; ENSG00000257594 [Q8N4A0-1] |
GeneIDi | 100528030 8693 |
KEGGi | hsa:100528030 hsa:8693 |
UCSCi | uc001tbd.4, human [Q8N4A0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Polypeptide N-acetylgalactosaminyltransferase 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y08564 Genomic DNA Translation: CAA69875.1 AK297677 mRNA Translation: BAG60038.1 AK312870 mRNA Translation: BAG35722.1 AC010201 Genomic DNA No translation available. AC025034 Genomic DNA No translation available. BC036390 mRNA Translation: AAH36390.1 |
CCDSi | CCDS53817.1 [Q8N4A0-1] |
RefSeqi | NP_001186711.1, NM_001199782.1 [Q8N4A0-2] NP_003765.2, NM_003774.4 [Q8N4A0-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5NQA | X-ray | 1.90 | A/B | 1-578 | [»] | |
6H0B | X-ray | 1.80 | A/B | 1-578 | [»] | |
SMRi | Q8N4A0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114240, 9 interactors 1529357, 1 interactor |
IntActi | Q8N4A0, 9 interactors |
STRINGi | 9606.ENSP00000436604 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
PTM databases
GlyGeni | Q8N4A0, 2 sites, 1 O-linked glycan (1 site) |
iPTMneti | Q8N4A0 |
PhosphoSitePlusi | Q8N4A0 |
SwissPalmi | Q8N4A0 |
Genetic variation databases
BioMutai | GALNT4 |
DMDMi | 338817878 |
Proteomic databases
EPDi | Q8N4A0 |
jPOSTi | Q8N4A0 |
MassIVEi | Q8N4A0 |
MaxQBi | Q8N4A0 |
PaxDbi | Q8N4A0 |
PeptideAtlasi | Q8N4A0 |
PRIDEi | Q8N4A0 |
ProteomicsDBi | 4651 71900 [Q8N4A0-1] |
Protocols and materials databases
Antibodypediai | 53055, 151 antibodies |
DNASUi | 8693 |
Genome annotation databases
Ensembli | ENST00000529983; ENSP00000436604; ENSG00000257594 [Q8N4A0-1] |
GeneIDi | 100528030 8693 |
KEGGi | hsa:100528030 hsa:8693 |
UCSCi | uc001tbd.4, human [Q8N4A0-1] |
Organism-specific databases
CTDi | 100528030 8693 |
DisGeNETi | 100528030 8693 |
GeneCardsi | GALNT4 |
HGNCi | HGNC:4126, GALNT4 |
HPAi | ENSG00000257594, Low tissue specificity |
MIMi | 603565, gene |
neXtProti | NX_Q8N4A0 |
OpenTargetsi | ENSG00000257594 |
PharmGKBi | PA28539 |
VEuPathDBi | HostDB:ENSG00000257594.3 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000163607 |
HOGENOMi | CLU_013477_0_1_1 |
InParanoidi | Q8N4A0 |
OMAi | MQTGEPM |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q8N4A0 |
TreeFami | TF352660 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.41, 2681 |
PathwayCommonsi | Q8N4A0 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
Miscellaneous databases
BioGRID-ORCSi | 100528030, 31 hits in 907 CRISPR screens 8693, 5 hits in 984 CRISPR screens |
Pharosi | Q8N4A0, Tbio |
PROi | PR:Q8N4A0 |
RNActi | Q8N4A0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000257594, Expressed in endometrium and 119 other tissues |
Genevisiblei | Q8N4A0, HS |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GALT4_HUMAN | |
Accessioni | Q8N4A0Primary (citable) accession number: Q8N4A0 Secondary accession number(s): B2R775, B4DMX6, O00208 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | June 28, 2011 | |
Last modified: | April 7, 2021 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families