UniProtKB - Q8N3T1 (GLT15_HUMAN)
Protein
Polypeptide N-acetylgalactosaminyltransferase 15
Gene
GALNT15
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Although it displays a much weaker activity toward all substrates tested compared to GALNT2, it is able to transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting that it can fill vicinal Thr/Ser residues in cooperation with other GALNT proteins. Prefers Muc1a as substrate.1 Publication
Caution
Was originally termed Galnt15/pp-GaNTase 15.1 Publication
Catalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDP1 PublicationEC:2.4.1.411 Publication
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDP1 PublicationEC:2.4.1.411 Publication
Cofactori
Mn2+By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 231 | SubstrateBy similarity | 1 | |
Binding sitei | 260 | SubstrateBy similarity | 1 | |
Metal bindingi | 283 | ManganeseBy similarity | 1 | |
Metal bindingi | 285 | ManganeseBy similarity | 1 | |
Metal bindingi | 417 | ManganeseBy similarity | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- O-glycan processing Source: Reactome
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q8N3T1 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: Polypeptide N-acetylgalactosaminyltransferase 15 (EC:2.4.1.411 Publication)Alternative name(s): Polypeptide GalNAc transferase-like protein 2 Short name: GalNAc-T-like protein 2 Short name: pp-GaNTase-like protein 2 Polypeptide N-acetylgalactosaminyltransferase-like protein 2 Protein-UDP acetylgalactosaminyltransferase-like protein 2 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 2 |
Gene namesi | Name:GALNT15 Synonyms:GALNTL2 ORF Names:UNQ770/PRO1564 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000131386.17 |
HGNCi | HGNC:21531, GALNT15 |
MIMi | 615131, gene |
neXtProti | NX_Q8N3T1 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
- transport vesicle Source: LIFEdb
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 34 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 35 – 639 | LumenalSequence analysisAdd BLAST | 605 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 117248 |
OpenTargetsi | ENSG00000131386 |
PharmGKBi | PA134936170 |
Miscellaneous databases
Pharosi | Q8N3T1, Tdark |
Polymorphism and mutation databases
BioMutai | GALNT15 |
DMDMi | 51316023 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059137 | 1 – 639 | Polypeptide N-acetylgalactosaminyltransferase 15Add BLAST | 639 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 107 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 181 ↔ 412 | PROSITE-ProRule annotation | ||
Disulfide bondi | 403 ↔ 482 | PROSITE-ProRule annotation | ||
Disulfide bondi | 517 ↔ 536 | PROSITE-ProRule annotation | ||
Disulfide bondi | 562 ↔ 575 | PROSITE-ProRule annotation | ||
Glycosylationi | 574 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 603 ↔ 620 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | Q8N3T1 |
MassIVEi | Q8N3T1 |
PaxDbi | Q8N3T1 |
PeptideAtlasi | Q8N3T1 |
PRIDEi | Q8N3T1 |
ProteomicsDBi | 71833 |
PTM databases
GlyGeni | Q8N3T1, 2 sites |
PhosphoSitePlusi | Q8N3T1 |
Expressioni
Tissue specificityi
Widely expressed. Highly expressed in small intestine, placenta, spleen, cerebral cortex and ovary. Expressed at intermediate level in uterus, mammary gland, stomach, cerebellum and whole brain. Weakly expressed in fetal brain, bone marrow, thyroid gland, thymus, heart, skeletal muscle, lung, liver, colon, pancreas, kidney and testis. Not expressed in leukocyte. Expressed in both normal and osteoarthritic cartilage. Expressed at low level in chondrocytes in all zones of both normal and osteoarthritic cartilage.2 Publications
Gene expression databases
Bgeei | ENSG00000131386, Expressed in spinal cord and 195 other tissues |
ExpressionAtlasi | Q8N3T1, baseline and differential |
Genevisiblei | Q8N3T1, HS |
Organism-specific databases
HPAi | ENSG00000131386, Tissue enhanced (adipose tissue, brain) |
Interactioni
Binary interactionsi
Hide detailsQ8N3T1
Protein-protein interaction databases
BioGRIDi | 125583, 17 interactors |
IntActi | Q8N3T1, 17 interactors |
STRINGi | 9606.ENSP00000344260 |
Miscellaneous databases
RNActi | Q8N3T1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 504 – 631 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 128 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 190 – 299 | Catalytic subdomain AAdd BLAST | 110 | |
Regioni | 358 – 420 | Catalytic subdomain BAdd BLAST | 63 |
Domaini
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000160808 |
HOGENOMi | CLU_013477_0_3_1 |
InParanoidi | Q8N3T1 |
OMAi | IILCFHD |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q8N3T1 |
TreeFami | TF313267 |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q8N3T1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLRKRYRHR PCRLQFLLLL LMLGCVLMMV AMLHPPHHTL HQTVTAQASK
60 70 80 90 100
HSPEARYRLD FGESQDWVLE AEDEGEEYSP LEGLPPFISL REDQLLVAVA
110 120 130 140 150
LPQARRNQSQ GRRGGSYRLI KQPRRQDKEA PKRDWGADED GEVSEEEELT
160 170 180 190 200
PFSLDPRGLQ EALSARIPLQ RALPEVRHPL CLQQHPQDSL PTASVILCFH
210 220 230 240 250
DEAWSTLLRT VHSILDTVPR AFLKEIILVD DLSQQGQLKS ALSEYVARLE
260 270 280 290 300
GVKLLRSNKR LGAIRARMLG ATRATGDVLV FMDAHCECHP GWLEPLLSRI
310 320 330 340 350
AGDRSRVVSP VIDVIDWKTF QYYPSKDLQR GVLDWKLDFH WEPLPEHVRK
360 370 380 390 400
ALQSPISPIR SPVVPGEVVA MDRHYFQNTG AYDSLMSLRG GENLELSFKA
410 420 430 440 450
WLCGGSVEIL PCSRVGHIYQ NQDSHSPLDQ EATLRNRVRI AETWLGSFKE
460 470 480 490 500
TFYKHSPEAF SLSKAEKPDC MERLQLQRRL GCRTFHWFLA NVYPELYPSE
510 520 530 540 550
PRPSFSGKLH NTGLGLCADC QAEGDILGCP MVLAPCSDSR QQQYLQHTSR
560 570 580 590 600
KEIHFGSPQH LCFAVRQEQV ILQNCTEEGL AIHQQHWDFQ ENGMIVHILS
610 620 630
GKCMEAVVQE NNKDLYLRPC DGKARQQWRF DQINAVDER
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JGI4 | C9JGI4_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT15 | 617 | Annotation score: | ||
Q4G146 | Q4G146_HUMAN | GALNTL2 protein | GALNT15 GALNTL2 | 196 | Annotation score: | ||
A0A3B3IRY6 | A0A3B3IRY6_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT15 | 83 | Annotation score: | ||
H7BZD6 | H7BZD6_HUMAN | Polypeptide N-acetylgalactosaminylt... | GALNT15 | 141 | Annotation score: |
Sequence cautioni
The sequence CAD89983 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 44 | V → D in CAD38585 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 326 | K → R in CAD89983 (PubMed:17974005).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049243 | 68 | V → G. Corresponds to variant dbSNP:rs36026882Ensembl. | 1 | |
Natural variantiVAR_049244 | 151 | P → L. Corresponds to variant dbSNP:rs11715981Ensembl. | 1 | |
Natural variantiVAR_049245 | 324 | P → A. Corresponds to variant dbSNP:rs12634179Ensembl. | 1 | |
Natural variantiVAR_049246 | 432 | A → T2 PublicationsCorresponds to variant dbSNP:rs17851238Ensembl. | 1 | |
Natural variantiVAR_019593 | 510 | H → Y. Corresponds to variant dbSNP:rs2271077Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB078149 mRNA Translation: BAD29961.1 AY035399 mRNA Translation: AAK63127.1 AY358443 mRNA Translation: AAQ88808.1 AK312425 mRNA Translation: BAG35335.1 AL831925 mRNA Translation: CAD38585.1 AL832575 mRNA Translation: CAD89983.1 Frameshift. AC087858 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW64266.1 BC014789 mRNA Translation: AAH14789.1 |
CCDSi | CCDS33711.1 |
RefSeqi | NP_473451.3, NM_054110.4 |
Genome annotation databases
Ensembli | ENST00000339732; ENSP00000344260; ENSG00000131386 |
GeneIDi | 117248 |
KEGGi | hsa:117248 |
UCSCi | uc003car.5, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Polypeptide N-acetylgalactosaminyltransferase-like protein 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB078149 mRNA Translation: BAD29961.1 AY035399 mRNA Translation: AAK63127.1 AY358443 mRNA Translation: AAQ88808.1 AK312425 mRNA Translation: BAG35335.1 AL831925 mRNA Translation: CAD38585.1 AL832575 mRNA Translation: CAD89983.1 Frameshift. AC087858 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW64266.1 BC014789 mRNA Translation: AAH14789.1 |
CCDSi | CCDS33711.1 |
RefSeqi | NP_473451.3, NM_054110.4 |
3D structure databases
SMRi | Q8N3T1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 125583, 17 interactors |
IntActi | Q8N3T1, 17 interactors |
STRINGi | 9606.ENSP00000344260 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
PTM databases
GlyGeni | Q8N3T1, 2 sites |
PhosphoSitePlusi | Q8N3T1 |
Polymorphism and mutation databases
BioMutai | GALNT15 |
DMDMi | 51316023 |
Proteomic databases
EPDi | Q8N3T1 |
MassIVEi | Q8N3T1 |
PaxDbi | Q8N3T1 |
PeptideAtlasi | Q8N3T1 |
PRIDEi | Q8N3T1 |
ProteomicsDBi | 71833 |
Protocols and materials databases
Antibodypediai | 2430, 61 antibodies |
DNASUi | 117248 |
Genome annotation databases
Ensembli | ENST00000339732; ENSP00000344260; ENSG00000131386 |
GeneIDi | 117248 |
KEGGi | hsa:117248 |
UCSCi | uc003car.5, human |
Organism-specific databases
CTDi | 117248 |
DisGeNETi | 117248 |
EuPathDBi | HostDB:ENSG00000131386.17 |
GeneCardsi | GALNT15 |
HGNCi | HGNC:21531, GALNT15 |
HPAi | ENSG00000131386, Tissue enhanced (adipose tissue, brain) |
MIMi | 615131, gene |
neXtProti | NX_Q8N3T1 |
OpenTargetsi | ENSG00000131386 |
PharmGKBi | PA134936170 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3736, Eukaryota |
GeneTreei | ENSGT00940000160808 |
HOGENOMi | CLU_013477_0_3_1 |
InParanoidi | Q8N3T1 |
OMAi | IILCFHD |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q8N3T1 |
TreeFami | TF313267 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
PathwayCommonsi | Q8N3T1 |
Reactomei | R-HSA-913709, O-linked glycosylation of mucins |
Miscellaneous databases
BioGRID-ORCSi | 117248, 1 hit in 838 CRISPR screens |
ChiTaRSi | GALNT15, human |
GeneWikii | GALNTL2 |
GenomeRNAii | 117248 |
Pharosi | Q8N3T1, Tdark |
PROi | PR:Q8N3T1 |
RNActi | Q8N3T1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000131386, Expressed in spinal cord and 195 other tissues |
ExpressionAtlasi | Q8N3T1, baseline and differential |
Genevisiblei | Q8N3T1, HS |
Family and domain databases
CDDi | cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLT15_HUMAN | |
Accessioni | Q8N3T1Primary (citable) accession number: Q8N3T1 Secondary accession number(s): A6NMN1 Q96DJ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | August 16, 2004 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations