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Protein

Vacuolar protein sorting-associated protein 8 homolog

Gene

VPS8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:25266290). Functions predominantly in APPL1-containing endosomes (PubMed:25266290).1 Publication1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1258 – 1310RING-type; atypicalPROSITE-ProRule annotationAdd BLAST53

GO - Molecular functioni

GO - Biological processi

  • endosomal vesicle fusion Source: UniProtKB
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transport
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 8 homolog
Gene namesi
Name:VPS8
Synonyms:KIAA0804
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000156931.15
HGNCiHGNC:29122 VPS8
neXtProtiNX_Q8N3P4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi23355
OpenTargetsiENSG00000156931
PharmGKBiPA142671617
PA142671888

Polymorphism and mutation databases

BioMutaiVPS8
DMDMi296452997

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782671 – 1428Vacuolar protein sorting-associated protein 8 homologAdd BLAST1428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineCombined sources1
Modified residuei32PhosphoserineCombined sources1
Modified residuei127PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8N3P4
MaxQBiQ8N3P4
PaxDbiQ8N3P4
PeptideAtlasiQ8N3P4
PRIDEiQ8N3P4
ProteomicsDBi71820
71821 [Q8N3P4-2]
71822 [Q8N3P4-3]

PTM databases

iPTMnetiQ8N3P4
PhosphoSitePlusiQ8N3P4

Expressioni

Gene expression databases

BgeeiENSG00000156931 Expressed in 220 organ(s), highest expression level in corpus callosum
CleanExiHS_VPS8
ExpressionAtlasiQ8N3P4 baseline and differential
GenevisibleiQ8N3P4 HS

Organism-specific databases

HPAiHPA036871

Interactioni

Subunit structurei

Interacts with RAB5C (By similarity). Interacts with TGFBRAP1 (PubMed:25266290). Component of the putative class C core vacuole/endosome tethering (CORVET) complex; the core of which composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, is associated with VPS8 and TGFBRAP1 (PubMed:25266290).By similarity1 Publication1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TGFBRAP1Q8WUH22EBI-7261494,EBI-2954829

Protein-protein interaction databases

BioGridi116937, 20 interactors
CORUMiQ8N3P4
IntActiQ8N3P4, 10 interactors
MINTiQ8N3P4
STRINGi9606.ENSP00000397879

Structurei

3D structure databases

ProteinModelPortaliQ8N3P4
SMRiQ8N3P4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati195 – 236WDAdd BLAST42

Sequence similaritiesi

Belongs to the VPS8 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1258 – 1310RING-type; atypicalPROSITE-ProRule annotationAdd BLAST53

Keywords - Domaini

WD repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2079 Eukaryota
ENOG410XRX6 LUCA
GeneTreeiENSGT00390000010672
HOGENOMiHOG000013171
HOVERGENiHBG071091
InParanoidiQ8N3P4
KOiK20178
PhylomeDBiQ8N3P4
TreeFamiTF314244

Family and domain databases

Gene3Di2.130.10.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR011044 Quino_amine_DH_bsu
IPR025941 Vps8_central_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF12816 Vps8, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SUPFAMiSSF50969 SSF50969, 2 hits
SSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8N3P4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MENEPDHENV EQSLCAKTSE EELNKSFNLE ASLSKFSYID MDKELEFKND
60 70 80 90 100
LIDDKEFDIP QVDTPPTLES ILNETDDEDE SFILEDPTLL NIDTIDSHSY
110 120 130 140 150
DTSSVASSDS GDRTNLKRKK KLPDSFSLHG SVMRHSLLKG ISAQIVSAAD
160 170 180 190 200
KVDAGLPTAI AVSSLIAVGT SHGLALIFGK DQNQALRLCL GSTSVGGQYG
210 220 230 240 250
AISALSINND CSRLLCGFAK GQITMWDLAS GKLLRSITDA HPPGTAILHI
260 270 280 290 300
KFTDDPTLAI CNDSGGSVFE LTFKRVMGVR TCESRCLFSG SKGEVCCIEP
310 320 330 340 350
LHSKPELKDH PITQFSLLAM ASLTKILVIG LKPSLKVWMT FPYGRMDPSS
360 370 380 390 400
VPLLAWHFVA VQNYVNPMLA FCRGDVVHFL LVKRDESGAI HVTKQKHLHL
410 420 430 440 450
YYDLINFTWI NSRTVVLLDS VEKLHVIDRQ TQEELETVEI SEVQLVYNSS
460 470 480 490 500
HFKSLATGGN VSQALALVGE KACYQSISSY GGQIFYLGTK SVYVMMLRSW
510 520 530 540 550
RERVDHLLKQ DCLTEALALA WSFHEGKAKA VVGLSGDASK RKAIVADRMV
560 570 580 590 600
EILFHYADRA LKKCPDQGKI QVMEQHFQDM VPVIVDYCLL LQRKDLLFSQ
610 620 630 640 650
MYDKLSENSV AKGVFLECLE PYILSDKLVG ITPQVMKDLI VHFQDKKLME
660 670 680 690 700
NVEALIVHMD ITSLDIQQVV LMCWENRLYD AMIYVYNRGM NEFISPMEKL
710 720 730 740 750
FRVIAPPLNA GKTLTDEQVV MGNKLLVYIS CCLAGRAYPL GDIPEDLVPL
760 770 780 790 800
VKNQVFEFLI RLHSAEASPE EEIYPYIRTL LHFDTREFLN VLALTFEDFK
810 820 830 840 850
NDKQAVEYQQ RIVDILLKVM VENSDFTPSQ VGCLFTFLAR QLAKPDNTLF
860 870 880 890 900
VNRTLFDQVL EFLCSPDDDS RHSERQQVLL ELLQAGGIVQ FEESRLIRMA
910 920 930 940 950
EKAEFYQICE FMYEREHQYD KIIDCYLRDP LREEEVFNYI HNILSIPGHS
960 970 980 990 1000
AEEKQSVWQK AMDHIEELVS LKPCKAAELV ATHFSGHIET VIKKLQNQVL
1010 1020 1030 1040 1050
LFKFLRSLLD PREGIHVNQE LLQISPCITE QFIELLCQFN PTQVIETLQV
1060 1070 1080 1090 1100
LECYRLEETI QITQKYQLHE VTAYLLEKKG DIHGAFLIML ERLQSKLQEV
1110 1120 1130 1140 1150
THQGENTKED PSLKDVEDTM VETIALCQRN SHNLNQQQRE ALWFPLLEAM
1160 1170 1180 1190 1200
MAPQKLSSSA IPHLHSEALK SLTMQVLNSM AAFIALPSIL QRILQDPVYG
1210 1220 1230 1240 1250
KGKLGEIQGL ILGMLDTFNY EQTLLETTTS LLNQDLHWSL CNLRASVTRG
1260 1270 1280 1290 1300
LNPKQDYCSI CLQQYKRRQE MADEIIVFSC GHLYHSFCLQ NKECTVEFEG
1310 1320 1330 1340 1350
QTRWTCYKCS SSNKVGKLSE NSSEIKKGRI TPSQVKMSPS YHQSKGDPTA
1360 1370 1380 1390 1400
KKGTSEPVLD PQQIQAFDQL CRLYRGSSRL ALLTELSQNR SSESYRPFSG
1410 1420
SQSAPAFNSI FQNENFQLQL IPPPVTED
Length:1,428
Mass (Da):161,754
Last modified:May 18, 2010 - v3
Checksum:i97FB5E8D453BBCB6
GO
Isoform 2 (identifier: Q8N3P4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-180: Missing.
     578-667: Missing.

Show »
Length:1,336
Mass (Da):151,216
Checksum:i67DBD2AE6D4FBEE6
GO
Isoform 3 (identifier: Q8N3P4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-180: Missing.

Note: No experimental confirmation available.
Show »
Length:1,426
Mass (Da):161,568
Checksum:iECF6DAC303CC7B16
GO

Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JJN9C9JJN9_HUMAN
Vacuolar protein sorting-associated...
VPS8
1,428Annotation score:
H7C058H7C058_HUMAN
Vacuolar protein sorting-associated...
VPS8
57Annotation score:
H7C322H7C322_HUMAN
Vacuolar protein sorting-associated...
VPS8
108Annotation score:
H7C1G7H7C1G7_HUMAN
Vacuolar protein sorting-associated...
VPS8
59Annotation score:
C9JKL0C9JKL0_HUMAN
Vacuolar protein sorting-associated...
VPS8
74Annotation score:
C9JIA0C9JIA0_HUMAN
Vacuolar protein sorting-associated...
VPS8
121Annotation score:
C9JP71C9JP71_HUMAN
Vacuolar protein sorting-associated...
VPS8
164Annotation score:
C9JPI1C9JPI1_HUMAN
Vacuolar protein sorting-associated...
VPS8
140Annotation score:
C9JG07C9JG07_HUMAN
Vacuolar protein sorting-associated...
VPS8
202Annotation score:
F8WB24F8WB24_HUMAN
Vacuolar protein sorting-associated...
VPS8
68Annotation score:
There is more potential isoformShow all

Sequence cautioni

The sequence BAB14322 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAH56195 differs from that shown. Reason: Frameshift at position 325.Curated
Isoform 2 : The sequence CAH56195 differs from that shown. Reason: Frameshift at position 326.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti433E → G in CAH56195 (PubMed:17974005).Curated1
Sequence conflicti471K → N in CAH56195 (PubMed:17974005).Curated1
Sequence conflicti765A → S in BAB14322 (PubMed:14702039).Curated1
Sequence conflicti932Missing in CAH56195 (PubMed:17974005).Curated1
Sequence conflicti1042T → A in BAB14322 (PubMed:14702039).Curated1
Sequence conflicti1043Q → L in CAH56195 (PubMed:17974005).Curated1
Sequence conflicti1167E → G in BAB14322 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03073083I → V1 PublicationCorresponds to variant dbSNP:rs9830734Ensembl.1
Natural variantiVAR_0307311165H → Y1 PublicationCorresponds to variant dbSNP:rs11555405Ensembl.1
Natural variantiVAR_0307321364I → T. Corresponds to variant dbSNP:rs3821750Ensembl.1
Natural variantiVAR_0307331372R → H. Corresponds to variant dbSNP:rs16859527Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023249179 – 180Missing in isoform 2 and isoform 3. 2 Publications2
Alternative sequenceiVSP_023250578 – 667Missing in isoform 2. 1 PublicationAdd BLAST90

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK022945 mRNA Translation: BAB14322.1 Different initiation.
AK292423 mRNA Translation: BAF85112.1
AL833838 mRNA Translation: CAD38698.1
BX647915 mRNA Translation: CAH56195.1 Frameshift.
AC025573 Genomic DNA No translation available.
AC107294 Genomic DNA No translation available.
AC117436 Genomic DNA No translation available.
BC001001 mRNA Translation: AAH01001.2
BC140768 mRNA Translation: AAI40769.1
AB018347 mRNA Translation: BAA34524.1
CCDSiCCDS46971.1 [Q8N3P4-1]
CCDS46972.1 [Q8N3P4-3]
RefSeqiNP_001009921.1, NM_001009921.2 [Q8N3P4-1]
NP_056118.2, NM_015303.3 [Q8N3P4-3]
XP_005247308.1, XM_005247251.3
UniGeneiHs.269263

Genome annotation databases

EnsembliENST00000436792; ENSP00000404704; ENSG00000156931 [Q8N3P4-3]
ENST00000446204; ENSP00000405483; ENSG00000156931 [Q8N3P4-2]
ENST00000625842; ENSP00000487164; ENSG00000156931 [Q8N3P4-1]
GeneIDi23355
KEGGihsa:23355
UCSCiuc003fpb.2 human [Q8N3P4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK022945 mRNA Translation: BAB14322.1 Different initiation.
AK292423 mRNA Translation: BAF85112.1
AL833838 mRNA Translation: CAD38698.1
BX647915 mRNA Translation: CAH56195.1 Frameshift.
AC025573 Genomic DNA No translation available.
AC107294 Genomic DNA No translation available.
AC117436 Genomic DNA No translation available.
BC001001 mRNA Translation: AAH01001.2
BC140768 mRNA Translation: AAI40769.1
AB018347 mRNA Translation: BAA34524.1
CCDSiCCDS46971.1 [Q8N3P4-1]
CCDS46972.1 [Q8N3P4-3]
RefSeqiNP_001009921.1, NM_001009921.2 [Q8N3P4-1]
NP_056118.2, NM_015303.3 [Q8N3P4-3]
XP_005247308.1, XM_005247251.3
UniGeneiHs.269263

3D structure databases

ProteinModelPortaliQ8N3P4
SMRiQ8N3P4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116937, 20 interactors
CORUMiQ8N3P4
IntActiQ8N3P4, 10 interactors
MINTiQ8N3P4
STRINGi9606.ENSP00000397879

PTM databases

iPTMnetiQ8N3P4
PhosphoSitePlusiQ8N3P4

Polymorphism and mutation databases

BioMutaiVPS8
DMDMi296452997

Proteomic databases

EPDiQ8N3P4
MaxQBiQ8N3P4
PaxDbiQ8N3P4
PeptideAtlasiQ8N3P4
PRIDEiQ8N3P4
ProteomicsDBi71820
71821 [Q8N3P4-2]
71822 [Q8N3P4-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000436792; ENSP00000404704; ENSG00000156931 [Q8N3P4-3]
ENST00000446204; ENSP00000405483; ENSG00000156931 [Q8N3P4-2]
ENST00000625842; ENSP00000487164; ENSG00000156931 [Q8N3P4-1]
GeneIDi23355
KEGGihsa:23355
UCSCiuc003fpb.2 human [Q8N3P4-1]

Organism-specific databases

CTDi23355
DisGeNETi23355
EuPathDBiHostDB:ENSG00000156931.15
GeneCardsiVPS8
HGNCiHGNC:29122 VPS8
HPAiHPA036871
neXtProtiNX_Q8N3P4
OpenTargetsiENSG00000156931
PharmGKBiPA142671617
PA142671888
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2079 Eukaryota
ENOG410XRX6 LUCA
GeneTreeiENSGT00390000010672
HOGENOMiHOG000013171
HOVERGENiHBG071091
InParanoidiQ8N3P4
KOiK20178
PhylomeDBiQ8N3P4
TreeFamiTF314244

Miscellaneous databases

ChiTaRSiVPS8 human
GenomeRNAii23355
PROiPR:Q8N3P4

Gene expression databases

BgeeiENSG00000156931 Expressed in 220 organ(s), highest expression level in corpus callosum
CleanExiHS_VPS8
ExpressionAtlasiQ8N3P4 baseline and differential
GenevisibleiQ8N3P4 HS

Family and domain databases

Gene3Di2.130.10.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR011044 Quino_amine_DH_bsu
IPR025941 Vps8_central_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF12816 Vps8, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SUPFAMiSSF50969 SSF50969, 2 hits
SSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVPS8_HUMAN
AccessioniPrimary (citable) accession number: Q8N3P4
Secondary accession number(s): A8K8Q8
, B9EIQ1, C9JB61, O94896, Q63HP2, Q9BVP9, Q9H9B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 18, 2010
Last modified: September 12, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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