UniProtKB - Q8N1Q8 (THEM5_HUMAN)
Protein
Acyl-coenzyme A thioesterase THEM5
Gene
THEM5
Organism
Homo sapiens (Human)
Status
Functioni
Has acyl-CoA thioesterase activity towards long-chain (C16 and C18) fatty acyl-CoA substrates, with a preference for linoleoyl-CoA and other unsaturated long-chain fatty acid-CoA esters (PubMed:22586271). Plays an important role in mitochondrial fatty acid metabolism, and in remodeling of the mitochondrial lipid cardiolipin (PubMed:22586271). Required for normal mitochondrial function (PubMed:22586271).1 Publication
Catalytic activityi
- EC:3.1.2.21 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=2.4 µM for palmitoyl-CoA (C16:0)1 Publication
- KM=4.2 µM for stearoyl-CoA (C18:0)1 Publication
- KM=2.9 µM for oleoyl-CoA (C18:1)1 Publication
- KM=4.2 µM for linoleoyl-CoA (C18:2)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 167 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- myristoyl-CoA hydrolase activity Source: UniProtKB-EC
- palmitoyl-CoA hydrolase activity Source: UniProtKB
GO - Biological processi
- acyl-CoA metabolic process Source: Reactome
- cardiolipin acyl-chain remodeling Source: UniProtKB
- fatty acid metabolic process Source: UniProtKB-KW
- long-chain fatty-acyl-CoA metabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Enzyme and pathway databases
PathwayCommonsi | Q8N1Q8 |
Reactomei | R-HSA-77289, Mitochondrial Fatty Acid Beta-Oxidation |
SABIO-RKi | Q8N1Q8 |
Chemistry databases
SwissLipidsi | SLP:000000659 |
Names & Taxonomyi
Protein namesi | Recommended name: Acyl-coenzyme A thioesterase THEM5 (EC:3.1.2.21 Publication)Short name: Acyl-CoA thioesterase THEM5 Alternative name(s): Acyl-coenzyme A thioesterase 15 Thioesterase superfamily member 5 |
Gene namesi | Name:THEM5 Synonyms:ACOT15 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000196407.11 |
HGNCi | HGNC:26755, THEM5 |
MIMi | 615653, gene |
neXtProti | NX_Q8N1Q8 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 1 Publication
Mitochondrion
- mitochondrial matrix Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 160 | G → A: Strongly reduces enzyme activity. Abolishes enzyme activity; when associated with A-183. 1 Publication | 1 | |
Mutagenesisi | 167 | D → A: Abolishes enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 183 | T → A: Reduces enzyme activity. Abolishes enzyme activity; when associated with A-160. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 284486 |
OpenTargetsi | ENSG00000196407 |
PharmGKBi | PA142670814 |
Miscellaneous databases
Pharosi | Q8N1Q8, Tbio |
Polymorphism and mutation databases
BioMutai | THEM5 |
DMDMi | 145566964 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000284519 | 1 – 247 | Acyl-coenzyme A thioesterase THEM5Add BLAST | 247 |
Proteomic databases
MassIVEi | Q8N1Q8 |
PaxDbi | Q8N1Q8 |
PeptideAtlasi | Q8N1Q8 |
PRIDEi | Q8N1Q8 |
ProteomicsDBi | 71628 |
PTM databases
iPTMneti | Q8N1Q8 |
PhosphoSitePlusi | Q8N1Q8 |
Expressioni
Gene expression databases
Bgeei | ENSG00000196407, Expressed in zone of skin and 111 other tissues |
ExpressionAtlasi | Q8N1Q8, baseline and differential |
Organism-specific databases
HPAi | ENSG00000196407, Group enriched (skin, tongue) |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
Hide detailsQ8N1Q8
With | #Exp. | IntAct |
---|---|---|
MAGEA11 - isoform 2 [P43364-2] | 3 | EBI-10264970,EBI-10178634 |
SYNJ2BP [P57105] | 3 | EBI-10264970,EBI-1049004 |
Protein-protein interaction databases
BioGRIDi | 129888, 8 interactors |
IntActi | Q8N1Q8, 3 interactors |
STRINGi | 9606.ENSP00000357807 |
Miscellaneous databases
RNActi | Q8N1Q8, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8N1Q8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the THEM4/THEM5 thioesterase family.Curated
Phylogenomic databases
eggNOGi | KOG4781, Eukaryota |
GeneTreei | ENSGT00940000161937 |
HOGENOMi | CLU_072603_0_0_1 |
InParanoidi | Q8N1Q8 |
OMAi | QVEGQGY |
OrthoDBi | 1322443at2759 |
PhylomeDBi | Q8N1Q8 |
TreeFami | TF332518 |
Family and domain databases
InterProi | View protein in InterPro IPR029069, HotDog_dom_sf IPR006683, Thioestr_dom |
Pfami | View protein in Pfam PF03061, 4HBT, 1 hit |
SUPFAMi | SSF54637, SSF54637, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8N1Q8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIRRCFQVAA RLGHHRGLLE APRILPRLNP ASAFGSSTDS MFSRFLPEKT
60 70 80 90 100
DLKDYALPNA SWCSDMLSLY QEFLEKTKSS GWIKLPSFKS NRDHIRGLKL
110 120 130 140 150
PSGLAVSSDK GDCRIFTRCI QVEGQGFEYV IFFQPTQKKS VCLFQPGSYL
160 170 180 190 200
EGPPGFAHGG SLAAMMDETF SKTAFLAGEG LFTLSLNIRF KNLIPVDSLV
210 220 230 240
VMDVELDKIE DQKLYMSCIA HSRDQQTVYA KSSGVFLQLQ LEEESPQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0Y6P4 | H0Y6P4_HUMAN | Acyl-coenzyme A thioesterase THEM5 | THEM5 | 119 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031764 | 55 | Y → S. Corresponds to variant dbSNP:rs16833597Ensembl. | 1 | |
Natural variantiVAR_031765 | 197 | D → G. Corresponds to variant dbSNP:rs6587625Ensembl. | 1 | |
Natural variantiVAR_031766 | 206 | L → V2 PublicationsCorresponds to variant dbSNP:rs6587624Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK095283 mRNA Translation: BAC04521.1 AL450992 Genomic DNA No translation available. BC101610 mRNA Translation: AAI01611.1 BC112239 mRNA Translation: AAI12240.1 |
CCDSi | CCDS1005.1 |
RefSeqi | NP_872384.1, NM_182578.3 |
Genome annotation databases
Ensembli | ENST00000368817; ENSP00000357807; ENSG00000196407 |
GeneIDi | 284486 |
KEGGi | hsa:284486 |
UCSCi | uc021oyw.2, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK095283 mRNA Translation: BAC04521.1 AL450992 Genomic DNA No translation available. BC101610 mRNA Translation: AAI01611.1 BC112239 mRNA Translation: AAI12240.1 |
CCDSi | CCDS1005.1 |
RefSeqi | NP_872384.1, NM_182578.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4AE7 | X-ray | 1.45 | A | 35-247 | [»] | |
SMRi | Q8N1Q8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 129888, 8 interactors |
IntActi | Q8N1Q8, 3 interactors |
STRINGi | 9606.ENSP00000357807 |
Chemistry databases
SwissLipidsi | SLP:000000659 |
PTM databases
iPTMneti | Q8N1Q8 |
PhosphoSitePlusi | Q8N1Q8 |
Polymorphism and mutation databases
BioMutai | THEM5 |
DMDMi | 145566964 |
Proteomic databases
MassIVEi | Q8N1Q8 |
PaxDbi | Q8N1Q8 |
PeptideAtlasi | Q8N1Q8 |
PRIDEi | Q8N1Q8 |
ProteomicsDBi | 71628 |
Protocols and materials databases
Antibodypediai | 34076, 63 antibodies |
DNASUi | 284486 |
Genome annotation databases
Ensembli | ENST00000368817; ENSP00000357807; ENSG00000196407 |
GeneIDi | 284486 |
KEGGi | hsa:284486 |
UCSCi | uc021oyw.2, human |
Organism-specific databases
CTDi | 284486 |
DisGeNETi | 284486 |
EuPathDBi | HostDB:ENSG00000196407.11 |
GeneCardsi | THEM5 |
HGNCi | HGNC:26755, THEM5 |
HPAi | ENSG00000196407, Group enriched (skin, tongue) |
MIMi | 615653, gene |
neXtProti | NX_Q8N1Q8 |
OpenTargetsi | ENSG00000196407 |
PharmGKBi | PA142670814 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4781, Eukaryota |
GeneTreei | ENSGT00940000161937 |
HOGENOMi | CLU_072603_0_0_1 |
InParanoidi | Q8N1Q8 |
OMAi | QVEGQGY |
OrthoDBi | 1322443at2759 |
PhylomeDBi | Q8N1Q8 |
TreeFami | TF332518 |
Enzyme and pathway databases
PathwayCommonsi | Q8N1Q8 |
Reactomei | R-HSA-77289, Mitochondrial Fatty Acid Beta-Oxidation |
SABIO-RKi | Q8N1Q8 |
Miscellaneous databases
BioGRID-ORCSi | 284486, 12 hits in 842 CRISPR screens |
GenomeRNAii | 284486 |
Pharosi | Q8N1Q8, Tbio |
PROi | PR:Q8N1Q8 |
RNActi | Q8N1Q8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000196407, Expressed in zone of skin and 111 other tissues |
ExpressionAtlasi | Q8N1Q8, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR029069, HotDog_dom_sf IPR006683, Thioestr_dom |
Pfami | View protein in Pfam PF03061, 4HBT, 1 hit |
SUPFAMi | SSF54637, SSF54637, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THEM5_HUMAN | |
Accessioni | Q8N1Q8Primary (citable) accession number: Q8N1Q8 Secondary accession number(s): Q5T1C3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 17, 2007 |
Last sequence update: | April 17, 2007 | |
Last modified: | December 2, 2020 | |
This is version 132 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations