UniProtKB - Q8N1K5 (THMS1_HUMAN)
Protein THEMIS
THEMIS
Functioni
Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
By similarityGO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- negative T cell selection Source: UniProtKB
- positive T cell selection Source: UniProtKB
- T cell receptor signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Developmental protein |
Biological process | Adaptive immunity, Immunity |
Enzyme and pathway databases
PathwayCommonsi | Q8N1K5 |
SignaLinki | Q8N1K5 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein THEMISAlternative name(s): Thymocyte-expressed molecule involved in selection |
Gene namesi | Name:THEMIS Synonyms:C6orf190, C6orf207 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:21569, THEMIS |
MIMi | 613607, gene |
neXtProti | NX_Q8N1K5 |
VEuPathDBi | HostDB:ENSG00000172673 |
Subcellular locationi
Nucleus
- COP9 signalosome Source: UniProtKB
- nucleus Source: UniProtKB
Other locations
- cell-cell junction Source: Ensembl
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 387357 |
OpenTargetsi | ENSG00000172673 |
PharmGKBi | PA165618330 |
Miscellaneous databases
Pharosi | Q8N1K5, Tbio |
Genetic variation databases
BioMutai | THEMIS |
DMDMi | 150421530 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000252378 | 1 – 641 | Protein THEMISAdd BLAST | 641 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 584 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q8N1K5 |
MassIVEi | Q8N1K5 |
MaxQBi | Q8N1K5 |
PeptideAtlasi | Q8N1K5 |
PRIDEi | Q8N1K5 |
ProteomicsDBi | 25677 71609 [Q8N1K5-1] 71610 [Q8N1K5-2] 71611 [Q8N1K5-3] |
PTM databases
iPTMneti | Q8N1K5 |
PhosphoSitePlusi | Q8N1K5 |
Expressioni
Gene expression databases
Bgeei | ENSG00000172673, Expressed in thymus and 124 other tissues |
ExpressionAtlasi | Q8N1K5, baseline and differential |
Genevisiblei | Q8N1K5, HS |
Organism-specific databases
HPAi | ENSG00000172673, Tissue enriched (lymphoid) |
Interactioni
Subunit structurei
Interacts with PLCG1, ITK, GRB2, and LAT.
By similarityBinary interactionsi
Q8N1K5
Isoform 1 [Q8N1K5-1]
With | #Exp. | IntAct |
---|---|---|
GRB2 [P62993] | 10 | EBI-15102259,EBI-401755 |
LCK [P06239] | 3 | EBI-15102259,EBI-1348 |
PLCG1 [P19174] | 3 | EBI-15102259,EBI-79387 |
ZAP70 [P43403] | 3 | EBI-15102259,EBI-1211276 |
Protein-protein interaction databases
BioGRIDi | 132289, 4 interactors |
IntActi | Q8N1K5, 13 interactors |
MINTi | Q8N1K5 |
STRINGi | 9606.ENSP00000357231 |
Miscellaneous databases
RNActi | Q8N1K5, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 259 | CABIT 1Add BLAST | 259 | |
Regioni | 260 – 518 | CABIT 2Add BLAST | 259 | |
Regioni | 614 – 641 | DisorderedSequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QSJR, Eukaryota |
GeneTreei | ENSGT00530000063770 |
InParanoidi | Q8N1K5 |
OMAi | KVPVGCQ |
OrthoDBi | 337909at2759 |
PhylomeDBi | Q8N1K5 |
TreeFami | TF333479 |
Family and domain databases
InterProi | View protein in InterPro IPR025946, CABIT_dom IPR039671, THEMIS |
PANTHERi | PTHR15215, PTHR15215, 1 hit |
Pfami | View protein in Pfam PF12736, CABIT, 2 hits |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALSLEEFVH SLDLRTLPRV LEIQAGIYLE GSIYEMFGNE CCFSTGEVIK
60 70 80 90 100
ITGLKVKKII AEICEQIEGC ESLQPFELPM NFPGLFKIVA DKTPYLTMEE
110 120 130 140 150
ITRTIHIGPS RLGHPCFYHQ KDIKLENLII KQGEQIMLNS VEEIDGEIMV
160 170 180 190 200
SCAVARNHQT HSFNLPLSQE GEFYECEDER IYTLKEIVEW KIPKNRTRTV
210 220 230 240 250
NLTDFSNKWD STNPFPKDFY GTLILKPVYE IQGVMKFRKD IIRILPSLDV
260 270 280 290 300
EVKDITDSYD ANWFLQLLST EDLFEMTSKE FPIVTEVIEA PEGNHLPQSI
310 320 330 340 350
LQPGKTIVIH KKYQASRILA SEIRSNFPKR HFLIPTSYKG KFKRRPREFP
360 370 380 390 400
TAYDLEIAKS EKEPLHVVAT KAFHSPHDKL SSVSVGDQFL VHQSETTEVL
410 420 430 440 450
CEGIKKVVNV LACEKILKKS YEAALLPLYM EGGFVEVIHD KKQYPISELC
460 470 480 490 500
KQFRLPFNVK VSVRDLSIEE DVLAATPGLQ LEEDITDSYL LISDFANPTE
510 520 530 540 550
CWEIPVGRLN MTVQLVSNFS RDAEPFLVRT LVEEITEEQY YMMRRYESSA
560 570 580 590 600
SHPPPRPPKH PSVEETKLTL LTLAEERTVD LPKSPKRHHV DITKKLHPNQ
610 620 630 640
AGLDSKVLIG SQNDLVDEEK ERSNRGATAI AETFKNEKHQ K
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0D9SFD2 | A0A0D9SFD2_HUMAN | Protein THEMIS | THEMIS | 562 | Annotation score: | ||
X1WI26 | X1WI26_HUMAN | Protein THEMIS | THEMIS | 201 | Annotation score: | ||
A0A0G2JQP7 | A0A0G2JQP7_HUMAN | Protein THEMIS | THEMIS | 210 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 63 | I → V in BAF84735 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 72 | S → F in BAG53994 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 220 | Y → C in BAG53994 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 482 | E → K in BAG53994 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 510 | N → S in CAE45941 (PubMed:17974005).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027846 | 284 | V → G. Corresponds to variant dbSNP:rs11968051Ensembl. | 1 | |
Natural variantiVAR_027847 | 630 | I → V4 PublicationsCorresponds to variant dbSNP:rs675531Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037964 | 1 – 97 | Missing in isoform 3. CuratedAdd BLAST | 97 | |
Alternative sequenceiVSP_037965 | 1 – 35 | Missing in isoform 2. 1 PublicationAdd BLAST | 35 | |
Alternative sequenceiVSP_055714 | 586 | K → KAGVQWRDLGSLQPLPPGFK QFSASASHVAGITGTPHHVQ in isoform 4. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK094863 mRNA Translation: BAG52943.1 Different initiation. CH471051 Genomic DNA Translation: EAW48092.1 BC130516 mRNA Translation: AAI30517.1 AK124031 mRNA Translation: BAG53994.1 AK128377 mRNA Translation: BAG54669.1 AK292046 mRNA Translation: BAF84735.1 AL035470 Genomic DNA No translation available. AL356432 Genomic DNA No translation available. AL365224 Genomic DNA No translation available. AK097903 mRNA Translation: BAC05194.1 Sequence problems. BX640890 mRNA Translation: CAE45941.1 |
CCDSi | CCDS34534.1 [Q8N1K5-1] CCDS55055.1 [Q8N1K5-2] CCDS55056.1 [Q8N1K5-4] |
RefSeqi | NP_001010923.1, NM_001010923.2 [Q8N1K5-1] NP_001158157.1, NM_001164685.1 [Q8N1K5-4] NP_001158159.1, NM_001164687.1 [Q8N1K5-2] NP_001305460.1, NM_001318531.1 [Q8N1K5-3] XP_011534118.1, XM_011535816.1 [Q8N1K5-3] |
Genome annotation databases
Ensembli | ENST00000368248; ENSP00000357231; ENSG00000172673 ENST00000368250; ENSP00000357233; ENSG00000172673 ENST00000537166; ENSP00000439863; ENSG00000172673 [Q8N1K5-2] ENST00000610842; ENSP00000480630; ENSG00000275122 ENST00000613862; ENSP00000480967; ENSG00000275122 ENST00000614155; ENSP00000484170; ENSG00000275122 [Q8N1K5-2] ENST00000630369; ENSP00000487358; ENSG00000172673 [Q8N1K5-4] |
GeneIDi | 387357 |
KEGGi | hsa:387357 |
MANE-Selecti | ENST00000368248.5; ENSP00000357231.2; NM_001010923.3; NP_001010923.1 |
UCSCi | uc010kfb.3, human [Q8N1K5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK094863 mRNA Translation: BAG52943.1 Different initiation. CH471051 Genomic DNA Translation: EAW48092.1 BC130516 mRNA Translation: AAI30517.1 AK124031 mRNA Translation: BAG53994.1 AK128377 mRNA Translation: BAG54669.1 AK292046 mRNA Translation: BAF84735.1 AL035470 Genomic DNA No translation available. AL356432 Genomic DNA No translation available. AL365224 Genomic DNA No translation available. AK097903 mRNA Translation: BAC05194.1 Sequence problems. BX640890 mRNA Translation: CAE45941.1 |
CCDSi | CCDS34534.1 [Q8N1K5-1] CCDS55055.1 [Q8N1K5-2] CCDS55056.1 [Q8N1K5-4] |
RefSeqi | NP_001010923.1, NM_001010923.2 [Q8N1K5-1] NP_001158157.1, NM_001164685.1 [Q8N1K5-4] NP_001158159.1, NM_001164687.1 [Q8N1K5-2] NP_001305460.1, NM_001318531.1 [Q8N1K5-3] XP_011534118.1, XM_011535816.1 [Q8N1K5-3] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 132289, 4 interactors |
IntActi | Q8N1K5, 13 interactors |
MINTi | Q8N1K5 |
STRINGi | 9606.ENSP00000357231 |
PTM databases
iPTMneti | Q8N1K5 |
PhosphoSitePlusi | Q8N1K5 |
Genetic variation databases
BioMutai | THEMIS |
DMDMi | 150421530 |
Proteomic databases
jPOSTi | Q8N1K5 |
MassIVEi | Q8N1K5 |
MaxQBi | Q8N1K5 |
PeptideAtlasi | Q8N1K5 |
PRIDEi | Q8N1K5 |
ProteomicsDBi | 25677 71609 [Q8N1K5-1] 71610 [Q8N1K5-2] 71611 [Q8N1K5-3] |
Protocols and materials databases
Antibodypediai | 49883, 240 antibodies from 34 providers |
DNASUi | 387357 |
Genome annotation databases
Organism-specific databases
CTDi | 387357 |
DisGeNETi | 387357 |
GeneCardsi | THEMIS |
HGNCi | HGNC:21569, THEMIS |
HPAi | ENSG00000172673, Tissue enriched (lymphoid) |
MIMi | 613607, gene |
neXtProti | NX_Q8N1K5 |
OpenTargetsi | ENSG00000172673 |
PharmGKBi | PA165618330 |
VEuPathDBi | HostDB:ENSG00000172673 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSJR, Eukaryota |
GeneTreei | ENSGT00530000063770 |
InParanoidi | Q8N1K5 |
OMAi | KVPVGCQ |
OrthoDBi | 337909at2759 |
PhylomeDBi | Q8N1K5 |
TreeFami | TF333479 |
Enzyme and pathway databases
PathwayCommonsi | Q8N1K5 |
SignaLinki | Q8N1K5 |
Miscellaneous databases
BioGRID-ORCSi | 387357, 4 hits in 1006 CRISPR screens |
ChiTaRSi | THEMIS, human |
GeneWikii | Protein_THEMIS |
GenomeRNAii | 387357 |
Pharosi | Q8N1K5, Tbio |
PROi | PR:Q8N1K5 |
RNActi | Q8N1K5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000172673, Expressed in thymus and 124 other tissues |
ExpressionAtlasi | Q8N1K5, baseline and differential |
Genevisiblei | Q8N1K5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR025946, CABIT_dom IPR039671, THEMIS |
PANTHERi | PTHR15215, PTHR15215, 1 hit |
Pfami | View protein in Pfam PF12736, CABIT, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | THMS1_HUMAN | |
Accessioni | Q8N1K5Primary (citable) accession number: Q8N1K5 Secondary accession number(s): A1L4F0 Q6MZT7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | June 26, 2007 | |
Last modified: | February 23, 2022 | |
This is version 142 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families