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Protein

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Gene

CMTR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m7GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m7GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA].1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei218SubstrateCombined sources1 Publication1
Binding sitei234S-adenosyl-L-methionineCombined sources1 Publication1
Active sitei2391 Publication1
Active sitei3641 Publication1
Active sitei404Proton acceptor1 Publication1
Binding sitei439SubstrateCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: UniProtKB
  • cap1 mRNA methylation Source: UniProtKB
  • mRNA methylation Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processmRNA capping, mRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000137200-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (EC:2.1.1.571 Publication)
Alternative name(s):
Cap methyltransferase 1
Cap1 2'O-ribose methyltransferase 1
Short name:
MTr1
Short name:
hMTr1
FtsJ methyltransferase domain-containing protein 2
Interferon-stimulated gene 95 kDa protein
Short name:
ISG95
Gene namesi
Name:CMTR1
Synonyms:FTSJD2, KIAA0082, MTR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000137200.12
HGNCiHGNC:21077 CMTR1
MIMi616189 gene
neXtProtiNX_Q8N1G2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi203K → A: Reduces both mRNA cap binding and catalytic activity of the enzyme. 1 Publication1
Mutagenesisi228R → A: No effect. 1 Publication1
Mutagenesisi239K → A: Abolishes catalytic activity. 1 Publication1
Mutagenesisi364D → A: Abolishes catalytic activity. 1 Publication1
Mutagenesisi404K → A: Abolishes catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi23070
OpenTargetsiENSG00000137200
PharmGKBiPA162389052

Polymorphism and mutation databases

BioMutaiCMTR1
DMDMi74750894

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002512391 – 835Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1Add BLAST835

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineBy similarity1
Modified residuei31PhosphoserineBy similarity1
Modified residuei53PhosphoserineCombined sources1
Modified residuei66PhosphoserineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei108N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8N1G2
MaxQBiQ8N1G2
PaxDbiQ8N1G2
PeptideAtlasiQ8N1G2
PRIDEiQ8N1G2
ProteomicsDBi71600
TopDownProteomicsiQ8N1G2

PTM databases

iPTMnetiQ8N1G2
PhosphoSitePlusiQ8N1G2

Expressioni

Inductioni

By interferons alpha and beta, and by Vaccinia virus infection.3 Publications

Gene expression databases

BgeeiENSG00000137200
CleanExiHS_FTSJD2
ExpressionAtlasiQ8N1G2 baseline and differential
GenevisibleiQ8N1G2 HS

Organism-specific databases

HPAiHPA029954
HPA029979
HPA029980

Interactioni

Subunit structurei

Interacts with POLR2A (via C-terminus).1 Publication

Protein-protein interaction databases

BioGridi116703, 14 interactors
IntActiQ8N1G2, 5 interactors
MINTiQ8N1G2
STRINGi9606.ENSP00000362550

Structurei

Secondary structure

1835
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi143 – 146Combined sources4
Turni168 – 174Combined sources7
Beta strandi176 – 179Combined sources4
Helixi184 – 186Combined sources3
Beta strandi189 – 191Combined sources3
Helixi193 – 204Combined sources12
Helixi205 – 208Combined sources4
Helixi211 – 221Combined sources11
Helixi225 – 227Combined sources3
Turni228 – 231Combined sources4
Beta strandi232 – 234Combined sources3
Helixi235 – 246Combined sources12
Turni247 – 252Combined sources6
Helixi265 – 267Combined sources3
Beta strandi271 – 277Combined sources7
Helixi282 – 291Combined sources10
Beta strandi293 – 300Combined sources8
Helixi310 – 312Combined sources3
Turni313 – 316Combined sources4
Turni318 – 320Combined sources3
Beta strandi321 – 323Combined sources3
Beta strandi326 – 328Combined sources3
Helixi329 – 331Combined sources3
Helixi339 – 351Combined sources13
Turni352 – 355Combined sources4
Beta strandi358 – 363Combined sources6
Turni370 – 372Combined sources3
Helixi373 – 375Combined sources3
Helixi376 – 379Combined sources4
Helixi381 – 394Combined sources14
Beta strandi395 – 405Combined sources11
Helixi411 – 423Combined sources13
Beta strandi424 – 430Combined sources7
Beta strandi442 – 449Combined sources8
Helixi454 – 470Combined sources17
Beta strandi473 – 481Combined sources9
Helixi483 – 487Combined sources5
Helixi490 – 519Combined sources30
Helixi528 – 539Combined sources12

3D structure databases

ProteinModelPortaliQ8N1G2
SMRiQ8N1G2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 133G-patchPROSITE-ProRule annotationAdd BLAST47
Domaini231 – 450RrmJ-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST220
Domaini752 – 786WWPROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni203 – 207Substrate bindingCombined sources1 Publication5
Regioni277 – 283S-adenosyl-L-methionine bindingCombined sources1 Publication7
Regioni335 – 336S-adenosyl-L-methionine bindingCombined sources1 Publication2
Regioni374 – 376Substrate bindingCombined sources1 Publication3
Regioni727 – 835Interaction with POLR2A1 PublicationAdd BLAST109

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2 – 19Bipartite nuclear localization signalPROSITE-ProRule annotation1 PublicationAdd BLAST18

Phylogenomic databases

eggNOGiKOG3673 Eukaryota
ENOG410XP08 LUCA
GeneTreeiENSGT00530000063742
HOGENOMiHOG000021970
HOVERGENiHBG057271
KOiK14589
OMAiSRLFWEW
OrthoDBiEOG091G02K5
PhylomeDBiQ8N1G2
TreeFamiTF314897

Family and domain databases

InterProiView protein in InterPro
IPR030376 Cap_mRNA_MeTrfase_1
IPR000467 G_patch_dom
IPR025816 RrmJ-type_MeTrfase
IPR002877 rRNA_MeTrfase_FtsJ_dom
IPR029063 SAM-dependent_MTases
IPR001202 WW_dom
PANTHERiPTHR16121:SF0 PTHR16121:SF0, 1 hit
PfamiView protein in Pfam
PF01728 FtsJ, 1 hit
PF01585 G-patch, 1 hit
SMARTiView protein in SMART
SM00443 G_patch, 1 hit
SM00456 WW, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS50174 G_PATCH, 1 hit
PS51613 SAM_MT_RRMJ, 1 hit
PS01159 WW_DOMAIN_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q8N1G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRRTDPECT APIKKQKKRV AELALSLSST SDDEPPSSVS HGAKASTTSL
60 70 80 90 100
SGSDSETEGK QHSSDSFDDA FKADSLVEGT SSRYSMYNSV SQKLMAKMGF
110 120 130 140 150
REGEGLGKYS QGRKDIVEAS SQKGRRGLGL TLRGFDQELN VDWRDEPEPS
160 170 180 190 200
ACEQVSWFPE CTTEIPDTQE MSDWMVVGKR KMIIEDETEF CGEELLHSVL
210 220 230 240 250
QCKSVFDVLD GEEMRRARTR ANPYEMIRGV FFLNRAAMKM ANMDFVFDRM
260 270 280 290 300
FTNPRDSYGK PLVKDREAEL LYFADVCAGP GGFSEYVLWR KKWHAKGFGM
310 320 330 340 350
TLKGPNDFKL EDFYSASSEL FEPYYGEGGI DGDGDITRPE NISAFRNFVL
360 370 380 390 400
DNTDRKGVHF LMADGGFSVE GQENLQEILS KQLLLCQFLM ALSIVRTGGH
410 420 430 440 450
FICKTFDLFT PFSVGLVYLL YCCFERVCLF KPITSRPANS ERYVVCKGLK
460 470 480 490 500
VGIDDVRDYL FAVNIKLNQL RNTDSDVNLV VPLEVIKGDH EFTDYMIRSN
510 520 530 540 550
ESHCSLQIKA LAKIHAFVQD TTLSEPRQAE IRKECLRLWG IPDQARVAPS
560 570 580 590 600
SSDPKSKFFE LIQGTEIDIF SYKPTLLTSK TLEKIRPVFD YRCMVSGSEQ
610 620 630 640 650
KFLIGLGKSQ IYTWDGRQSD RWIKLDLKTE LPRDTLLSVE IVHELKGEGK
660 670 680 690 700
AQRKISAIHI LDVLVLNGTD VREQHFNQRI QLAEKFVKAV SKPSRPDMNP
710 720 730 740 750
IRVKEVYRLE EMEKIFVRLE MKIIKGSSGT PKLSYTGRDD RHFVPMGLYI
760 770 780 790 800
VRTVNEPWTM GFSKSFKKKF FYNKKTKDST FDLPADSIAP FHICYYGRLF
810 820 830
WEWGDGIRVH DSQKPQDQDK LSKEDVLSFI QMHRA
Length:835
Mass (Da):95,321
Last modified:October 1, 2002 - v1
Checksum:i03919512A73C30FB
GO

Sequence cautioni

The sequence BAA07893 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti795Y → H in BAF85253 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43949 mRNA Translation: BAA07893.2 Different initiation.
AK292564 mRNA Translation: BAF85253.1
AL353597 Genomic DNA No translation available.
BC010731 mRNA Translation: AAH10731.2
BC031890 mRNA Translation: AAH31890.1
CCDSiCCDS4835.1
RefSeqiNP_055865.1, NM_015050.2
UniGeneiHs.520102

Genome annotation databases

EnsembliENST00000373451; ENSP00000362550; ENSG00000137200
GeneIDi23070
KEGGihsa:23070
UCSCiuc003ons.4 human

Similar proteinsi

Entry informationi

Entry nameiCMTR1_HUMAN
AccessioniPrimary (citable) accession number: Q8N1G2
Secondary accession number(s): A8K949, Q14670, Q96FJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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