UniProtKB - Q8MV48 (GALT7_DROME)
N-acetylgalactosaminyltransferase 7
Pgant7
Functioni
Glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide (PubMed:11925450, PubMed:12829714).
In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties (PubMed:11925450).
Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified. Prefers the monoglycosylated Muc5AC-3 as substrate (PubMed:11925450).
Might have a role in protein O-glycosylation in the Golgi and thereby in establishing and/or maintaining a proper secretory apparatus structure (PubMed:20807760).
3 PublicationsCatalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-seryl-[protein] + H+ + UDP2 PublicationsEC:2.4.1.412 Publications
- L-threonyl-[protein] + UDP-N-acetyl-α-D-galactosamine = 3-O-[N-acetyl-α-D-galactosaminyl]-L-threonyl-[protein] + H+ + UDP2 PublicationsEC:2.4.1.412 Publications
Cofactori
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.2 PublicationsView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 182 | SubstrateBy similarity | 1 | |
Binding sitei | 212 | SubstrateBy similarity | 1 | |
Metal bindingi | 235 | ManganeseBy similarity | 1 | |
Metal bindingi | 237 | ManganeseBy similarity | 1 | |
Binding sitei | 344 | SubstrateBy similarity | 1 | |
Metal bindingi | 372 | ManganeseBy similarity | 1 | |
Binding sitei | 375 | SubstrateBy similarity | 1 | |
Binding sitei | 380 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB
GO - Biological processi
- oligosaccharide biosynthetic process Source: UniProtKB
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Lectin, Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.4.1.41, 1994 |
Reactomei | R-DME-913709, O-linked glycosylation of mucins |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
Names & Taxonomyi
Protein namesi | Recommended name: N-acetylgalactosaminyltransferase 7 (EC:2.4.1.412 Publications)Alternative name(s): Protein-UDP acetylgalactosaminyltransferase 7 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7 Short name: pp-GaNTase 7 dGalNAc-T2 |
Gene namesi | ORF Names:CG6394 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0030930, Pgant7 |
VEuPathDBi | VectorBase:FBgn0030930 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: GO_Central
- Golgi membrane Source: UniProtKB-SubCell
- Golgi stack Source: UniProtKB
Other locations
- endomembrane system Source: FlyBase
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 29 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 18 | |
Topological domaini | 30 – 591 | LumenalSequence analysisAdd BLAST | 562 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059161 | 1 – 591 | N-acetylgalactosaminyltransferase 7Add BLAST | 591 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 30 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 132 ↔ 367 | PROSITE-ProRule annotation | ||
Disulfide bondi | 358 ↔ 441 | PROSITE-ProRule annotation | ||
Disulfide bondi | 479 ↔ 496 | PROSITE-ProRule annotation | ||
Disulfide bondi | 519 ↔ 532 | PROSITE-ProRule annotation | ||
Disulfide bondi | 558 ↔ 573 | PROSITE-ProRule annotation | ||
Glycosylationi | 576 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q8MV48 |
PRIDEi | Q8MV48 |
PTM databases
GlyGeni | Q8MV48, 2 sites |
SwissPalmi | Q8MV48 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | FBgn0030930, Expressed in saliva-secreting gland and 55 other tissues |
ExpressionAtlasi | Q8MV48, baseline and differential |
Genevisiblei | Q8MV48, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 59149, 4 interactors |
IntActi | Q8MV48, 4 interactors |
STRINGi | 7227.FBpp0074396 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 466 – 585 | Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST | 120 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 68 – 100 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 141 – 251 | Catalytic subdomain AAdd BLAST | 111 | |
Regioni | 313 – 375 | Catalytic subdomain BAdd BLAST | 63 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3737, Eukaryota |
HOGENOMi | CLU_013477_0_1_1 |
InParanoidi | Q8MV48 |
OMAi | FDDTHKE |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q8MV48 |
Family and domain databases
CDDi | cd02510, pp-GalNAc-T, 1 hit cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR045885, GalNAc-T IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MRVSTIRSGR ICRLALCLLV LLPLLYLLAN WSDHHKRVQE AYHTRFGGPK
60 70 80 90 100
FAHQRLEGRP REVPKLVDGL GNFEPKDVKP RSGPGENGEA HSLSPDKKHM
110 120 130 140 150
SDASEMEYGM NIACSDEISM HRSVRDTRLE ECRHWDYPFD LPRTSVIIVF
160 170 180 190 200
HNEGFSVLMR TVHSVIDRSP THMLHEIILV DDFSDKENLR SQLDEYVLQF
210 220 230 240 250
KGLVKVIRNK EREGLIRTRS RGAMEATGEV IVFLDAHCEV NTNWLPPLLA
260 270 280 290 300
PIYRDRTVMT VPIIDGIDHK NFEYRPVYGT DNHFRGIFEW GMLYKENEVP
310 320 330 340 350
RREQRRRAHN SEPYRSPTHA GGLFAINREY FLELGAYDPG LLVWGGENFE
360 370 380 390 400
LSFKIWQCGG SIEWVPCSRV GHVYRGFMPY NFGKLASKKK GPLITINYKR
410 420 430 440 450
VIETWFDDTH KEYFYTREPL ARYLDMGDIS EQLALKKRLN CKSFQWFMDH
460 470 480 490 500
IAYDVYDKFP GLPANLHWGE LRSVASDGCL DSMGHQPPAI MGLTYCHGGG
510 520 530 540 550
NNQLVRLNAA GQLGVGERCV EADRQGIKLA VCRLGTVDGP WQYNEHTKHL
560 570 580 590
MHRVHKKCMA LHPATQQLSL GHCDVNDSYQ QWWFKEIRPR W
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketX2JEA3 | X2JEA3_DROME | Polypeptide N-acetylgalactosaminylt... | Pgant7 anon-WO02059370.15, dGalNAc-T2, Dmel\CG6394, GalNAc-T2, PGANT7 | 591 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 | P → S in AAM62412 (PubMed:11925450).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF493067 mRNA Translation: AAM62412.1 AY268068 mRNA Translation: AAQ56704.1 AE014298 Genomic DNA Translation: AAF48851.1 AE014298 Genomic DNA Translation: AAN09470.1 BT016123 mRNA Translation: AAV37008.1 AY058660 mRNA Translation: AAL13889.1 Different initiation. AY061339 mRNA Translation: AAL28887.1 Different initiation. |
RefSeqi | NP_001285406.1, NM_001298477.1 NP_573301.2, NM_133073.3 NP_728178.1, NM_167623.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0074624; FBpp0074395; FBgn0030930 FBtr0074625; FBpp0074396; FBgn0030930 FBtr0346082; FBpp0311917; FBgn0030930 |
GeneIDi | 32836 |
KEGGi | dme:Dmel_CG6394 |
UCSCi | CG6394-RB, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF493067 mRNA Translation: AAM62412.1 AY268068 mRNA Translation: AAQ56704.1 AE014298 Genomic DNA Translation: AAF48851.1 AE014298 Genomic DNA Translation: AAN09470.1 BT016123 mRNA Translation: AAV37008.1 AY058660 mRNA Translation: AAL13889.1 Different initiation. AY061339 mRNA Translation: AAL28887.1 Different initiation. |
RefSeqi | NP_001285406.1, NM_001298477.1 NP_573301.2, NM_133073.3 NP_728178.1, NM_167623.2 |
3D structure databases
AlphaFoldDBi | Q8MV48 |
SMRi | Q8MV48 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 59149, 4 interactors |
IntActi | Q8MV48, 4 interactors |
STRINGi | 7227.FBpp0074396 |
Protein family/group databases
CAZyi | CBM13, Carbohydrate-Binding Module Family 13 GT27, Glycosyltransferase Family 27 |
PTM databases
GlyGeni | Q8MV48, 2 sites |
SwissPalmi | Q8MV48 |
Proteomic databases
PaxDbi | Q8MV48 |
PRIDEi | Q8MV48 |
Protocols and materials databases
DNASUi | 32836 |
Genome annotation databases
EnsemblMetazoai | FBtr0074624; FBpp0074395; FBgn0030930 FBtr0074625; FBpp0074396; FBgn0030930 FBtr0346082; FBpp0311917; FBgn0030930 |
GeneIDi | 32836 |
KEGGi | dme:Dmel_CG6394 |
UCSCi | CG6394-RB, d. melanogaster |
Organism-specific databases
CTDi | 32836 |
FlyBasei | FBgn0030930, Pgant7 |
VEuPathDBi | VectorBase:FBgn0030930 |
Phylogenomic databases
eggNOGi | KOG3737, Eukaryota |
HOGENOMi | CLU_013477_0_1_1 |
InParanoidi | Q8MV48 |
OMAi | FDDTHKE |
OrthoDBi | 606683at2759 |
PhylomeDBi | Q8MV48 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.41, 1994 |
Reactomei | R-DME-913709, O-linked glycosylation of mucins |
Miscellaneous databases
BioGRID-ORCSi | 32836, 0 hits in 1 CRISPR screen |
GenomeRNAii | 32836 |
PROi | PR:Q8MV48 |
Gene expression databases
Bgeei | FBgn0030930, Expressed in saliva-secreting gland and 55 other tissues |
ExpressionAtlasi | Q8MV48, baseline and differential |
Genevisiblei | Q8MV48, DM |
Family and domain databases
CDDi | cd02510, pp-GalNAc-T, 1 hit cd00161, RICIN, 1 hit |
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR045885, GalNAc-T IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans IPR035992, Ricin_B-like_lectins IPR000772, Ricin_B_lectin |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit PF00652, Ricin_B_lectin, 1 hit |
SMARTi | View protein in SMART SM00458, RICIN, 1 hit |
SUPFAMi | SSF50370, SSF50370, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS50231, RICIN_B_LECTIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GALT7_DROME | |
Accessioni | Q8MV48Primary (citable) accession number: Q8MV48 Secondary accession number(s): A4V4R2 Q9VWT6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | August 16, 2004 | |
Last modified: | May 25, 2022 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families