The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - Q8MSU3 (FRRS1_DROME)
Protein
Putative ferric-chelate reductase 1 homolog
Gene
CG8399
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Putative ferric-chelate reductases reduce Fe3+ to Fe2+ before its transport from the endosome to the cytoplasm.
1 PublicationCaution
The cytochrome b561 domain lacks the conserved His residue that binds iron in the heme. The reductase activity is therefore unsure in vivo.Curated
Cofactori
heme bBy similarityNote: Binds 2 heme b groups non-covalently.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 409 | Iron 1 (heme b 1 axial ligand); via tele nitrogenBy similarity | 1 | |
Metal bindingi | 450 | Iron 2 (heme b 2 axial ligand); via tele nitrogenBy similarity | 1 | |
Metal bindingi | 480 | Iron 1 (heme b 1 axial ligand); via tele nitrogenBy similarity | 1 | |
Metal bindingi | 516 | Iron 2 (heme b 2 axial ligand); via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- oxidoreductase activity, acting on metal ions Source: UniProtKB
- transmembrane ascorbate ferrireductase activity Source: FlyBase
GO - Biological processi
- cellular iron ion homeostasis Source: ARUK-UCL
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Transport |
Ligand | Iron, Metal-binding |
Protein family/group databases
TCDBi | 5.B.2.2.3, the eukaryotic cytochrome b561 (cytb561) family |
Names & Taxonomyi
Protein namesi | |
Gene namesi | ORF Names:CG8399 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0034067, CG8399 |
VEuPathDBi | VectorBase:FBgn0034067 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Other locations
- integral component of membrane Source: FlyBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 10 – 30 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Transmembranei | 408 – 428 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Transmembranei | 452 – 472 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Transmembranei | 480 – 500 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Transmembranei | 515 – 535 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Transmembranei | 548 – 568 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Transmembranei | 616 – 636 | Helical; Name=7Sequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000314847 | 1 – 647 | Putative ferric-chelate reductase 1 homologAdd BLAST | 647 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 127 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 158 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q8MSU3 |
PRIDEi | Q8MSU3 |
PTM databases
GlyGeni | Q8MSU3, 2 sites |
SwissPalmi | Q8MSU3 |
Expressioni
Gene expression databases
Bgeei | FBgn0034067, Expressed in second segment of antenna (Drosophila) and 35 other tissues |
ExpressionAtlasi | Q8MSU3, baseline and differential |
Genevisiblei | Q8MSU3, DM |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 195 | ReelinPROSITE-ProRule annotationAdd BLAST | 171 | |
Domaini | 245 – 368 | DOMONPROSITE-ProRule annotationAdd BLAST | 124 | |
Domaini | 372 – 570 | Cytochrome b561PROSITE-ProRule annotationAdd BLAST | 199 |
Sequence similaritiesi
Belongs to the FRRS1 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4293, Eukaryota |
GeneTreei | ENSGT00940000170494 |
HOGENOMi | CLU_028305_0_0_1 |
InParanoidi | Q8MSU3 |
OMAi | PLTMRSW |
PhylomeDBi | Q8MSU3 |
Family and domain databases
CDDi | cd08544, Reeler, 1 hit |
Gene3Di | 2.60.40.4060, 1 hit |
InterProi | View protein in InterPro IPR006593, Cyt_b561/ferric_Rdtase_TM IPR005018, DOMON_domain IPR002861, Reeler_dom IPR042307, Reeler_sf |
Pfami | View protein in Pfam PF02014, Reeler, 1 hit |
SMARTi | View protein in SMART SM00665, B561, 1 hit SM00664, DoH, 1 hit |
PROSITEi | View protein in PROSITE PS50939, CYTOCHROME_B561, 1 hit PS50836, DOMON, 1 hit PS51019, REELIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8MSU3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMQALTMRSW LATLVTALLA VAIWPDPGQS LPQGAPETVC DTMLPFHSGG
60 70 80 90 100
SVLPQNSVSP FSVETSSSTL GQGQTLRVDL TGVPAGLSFG GYMIQARNRN
110 120 130 140 150
PPHQIIGQFG PARDGTIKLM NCENSVNNSA THSNAGPKQQ VILEWQSPVD
160 170 180 190 200
FLGQVVFNAT IAQSYNEFWV GVPSQPVQIV RRDLSAAPPL PTQSPSAPAG
210 220 230 240 250
TTRAPYVPPS YVAPNNVVAV SSDPIYNGCG QSKNCFGFPD GCVATKSCTS
260 270 280 290 300
ITVVTVRGDV FEFEIQSGKG TNAAYVAVGL SDDAKMGDDL TTECVPENGR
310 320 330 340 350
VNLYSSLTSA SPYSAVRSNV NQNSARLLDA SIVDGVIYCR VQRDAVTNVQ
360 370 380 390 400
GRTFDLRNGK YHLLVASGSS LKENSVGYHD IGRLPSAQPI NLAEVQDLSG
410 420 430 440 450
SSRLLIQLHG AFMIAAWIGT TSLGIIFARY FKQTWVGSQS CGTDQWFAWH
460 470 480 490 500
RLLMVTTWSL TVAAYVLIWV ELKQAVWHAH SIIGLITVIL CFIQPIGALF
510 520 530 540 550
RPGPNDKKRP YFNWGHWLGG NLAHILGIVT IFFSVKLPKA ELPEWMDWIL
560 570 580 590 600
VSFVVVHVLV HLIFSIGGMA SERHLSQRAN TFQMGDMSHH QQHAMRNGMS
610 620 630 640
MERKMDAPYA GMRKGLLGVY GVVLILFVTV LILLVVLAPI EQFLGKS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4LGL5 | A0A0B4LGL5_DROME | Uncharacterized protein, isoform B | sdr2 anon-EST:ParkEST009, Dmel\CG8399, SDR2, CG8399, Dmel_CG8399 | 656 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF58074.2 AY118595 mRNA Translation: AAM49964.1 |
RefSeqi | NP_611079.2, NM_137235.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0087207; FBpp0086349; FBgn0034067 |
GeneIDi | 36768 |
KEGGi | dme:Dmel_CG8399 |
UCSCi | CG8399-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF58074.2 AY118595 mRNA Translation: AAM49964.1 |
RefSeqi | NP_611079.2, NM_137235.3 |
3D structure databases
AlphaFoldDBi | Q8MSU3 |
SMRi | Q8MSU3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7227.FBpp0086349 |
Protein family/group databases
TCDBi | 5.B.2.2.3, the eukaryotic cytochrome b561 (cytb561) family |
PTM databases
GlyGeni | Q8MSU3, 2 sites |
SwissPalmi | Q8MSU3 |
Proteomic databases
PaxDbi | Q8MSU3 |
PRIDEi | Q8MSU3 |
Protocols and materials databases
DNASUi | 36768 |
Genome annotation databases
EnsemblMetazoai | FBtr0087207; FBpp0086349; FBgn0034067 |
GeneIDi | 36768 |
KEGGi | dme:Dmel_CG8399 |
UCSCi | CG8399-RA, d. melanogaster |
Organism-specific databases
FlyBasei | FBgn0034067, CG8399 |
VEuPathDBi | VectorBase:FBgn0034067 |
Phylogenomic databases
eggNOGi | KOG4293, Eukaryota |
GeneTreei | ENSGT00940000170494 |
HOGENOMi | CLU_028305_0_0_1 |
InParanoidi | Q8MSU3 |
OMAi | PLTMRSW |
PhylomeDBi | Q8MSU3 |
Miscellaneous databases
BioGRID-ORCSi | 36768, 0 hits in 1 CRISPR screen |
GenomeRNAii | 36768 |
PROi | PR:Q8MSU3 |
Gene expression databases
Bgeei | FBgn0034067, Expressed in second segment of antenna (Drosophila) and 35 other tissues |
ExpressionAtlasi | Q8MSU3, baseline and differential |
Genevisiblei | Q8MSU3, DM |
Family and domain databases
CDDi | cd08544, Reeler, 1 hit |
Gene3Di | 2.60.40.4060, 1 hit |
InterProi | View protein in InterPro IPR006593, Cyt_b561/ferric_Rdtase_TM IPR005018, DOMON_domain IPR002861, Reeler_dom IPR042307, Reeler_sf |
Pfami | View protein in Pfam PF02014, Reeler, 1 hit |
SMARTi | View protein in SMART SM00665, B561, 1 hit SM00664, DoH, 1 hit |
PROSITEi | View protein in PROSITE PS50939, CYTOCHROME_B561, 1 hit PS50836, DOMON, 1 hit PS51019, REELIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FRRS1_DROME | |
Accessioni | Q8MSU3Primary (citable) accession number: Q8MSU3 Secondary accession number(s): Q9V7I1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | October 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families