UniProtKB - Q8MPM3 (Q8MPM3_LEIME)
Protein
Transketolase
Gene
TKT
Organism
Leishmania mexicana mexicana
Status
Functioni
Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.UniRule annotation
Catalytic activityi
- D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphateUniRule annotationARBA annotationEC:2.2.1.1UniRule annotationARBA annotation
Cofactori
Co2+ARBA annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 29 | Thiamine pyrophosphate 1Combined sources | 1 | |
Binding sitei | 66 | Thiamine pyrophosphate 1Combined sources | 1 | |
Metal bindingi | 155 | CalciumCombined sources | 1 | |
Binding sitei | 156 | Thiamine pyrophosphate 1; via amide nitrogenCombined sources | 1 | |
Metal bindingi | 185 | CalciumCombined sources | 1 | |
Metal bindingi | 187 | Calcium; via carbonyl oxygenCombined sources | 1 | |
Binding sitei | 261 | Thiamine pyrophosphate 1Combined sources | 1 | |
Binding sitei | 409 | Thiamine pyrophosphate 2Combined sources | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- transketolase activity Source: UniProtKB-EC
Keywordsi
Molecular function | TransferaseUniRule annotationARBA annotation |
Ligand | CalciumUniRule annotationCombined sources, MagnesiumUniRule annotationARBA annotation, Metal-bindingUniRule annotationARBA annotation, Thiamine pyrophosphateUniRule annotationARBA annotation |
Enzyme and pathway databases
BRENDAi | 2.2.1.1, 2951 |
Names & Taxonomyi
Protein namesi | Recommended name: TransketolaseUniRule annotationARBA annotation (EC:2.2.1.1UniRule annotationARBA annotation) |
Gene namesi | Name:TKTImported |
Organismi | Leishmania mexicana mexicanaImported |
Taxonomic identifieri | 44270 [NCBI] |
Taxonomic lineagei | Eukaryota › Discoba › Euglenozoa › Kinetoplastea › Metakinetoplastina › Trypanosomatida › Trypanosomatidae › Leishmaniinae › Leishmania › |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationARBA annotationStructurei
3D structure databases
SMRi | Q8MPM3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8MPM3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 32 | TRANSKETOLASE_1InterPro annotationAdd BLAST | 21 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 114 – 116 | Thiamine pyrophosphate 1 bindingCombined sources | 3 | |
Regioni | 185 – 187 | Thiamine pyrophosphate 1 bindingCombined sources | 3 | |
Regioni | 435 – 438 | Thiamine pyrophosphate 2 bindingCombined sources | 4 |
Sequence similaritiesi
Family and domain databases
CDDi | cd02012, TPP_TK, 1 hit |
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR005478, Transketolase_bac-like IPR020826, Transketolase_BS IPR033248, Transketolase_C IPR033247, Transketolase_fam IPR005474, Transketolase_N |
PANTHERi | PTHR43522, PTHR43522, 1 hit |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit PF00456, Transketolase_N, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
TIGRFAMsi | TIGR00232, tktlase_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00801, TRANSKETOLASE_1, 1 hit PS00802, TRANSKETOLASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8MPM3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASIEKVANC IRCLAADIVQ GGKSGHPGTP MGMAPMSAVL WTEVMKYNSQ
60 70 80 90 100
DPDWVDRDRF VMSNGHGCAL QYALLHMAGY NLTMDDLKGF RQDGSRTPGH
110 120 130 140 150
PERFVTPGVE VTTGPLGQGI ANAVGLAIAE AHLAATFNRP GYNIVDHYTY
160 170 180 190 200
VYCGDGCLME GVCQEALSLA GHLALEKLIV IYDSNYISID GSTSLSFTEQ
210 220 230 240 250
CHQKYVAMGF HVIEVKNGDT DYEGLRKALA EAKATKGKPK MIVQTTTIGF
260 270 280 290 300
GSSKQGTEKV HGAPLGEEDI ANIKAKFGRD PQKKYDVDDD VRAVFRMHID
310 320 330 340 350
KCSAEQKAWE ELLAKYTAAF PAEGAAFVAQ MRGELPSGWE AKLPTNSSAI
360 370 380 390 400
ATRKASENCL AVLFPAIPAL MGGSADLTPS NLTRPASANL VDFSSSSKEG
410 420 430 440 450
RYIRFGVREH AMCAILNGLD AHDGIIPFGG TFLNFIGYAL GAVRLAAISH
460 470 480 490 500
HRVIYVATHD SIGVGEDGPT HQPVELVAAL RAMPNLQVIR PSDQTETSGA
510 520 530 540 550
WAVALSSIHT PTVLCLSRQN TEPQSGSSIE GVRHGAYSVV DVPDLQLVIV
560 570 580 590 600
ASGSEVSLAV DAAKALSGEL RVRVVSMPCQ ELFDAQPDTY RQAVLPAGVP
610 620 630 640 650
VVSVEAYVSF GWEKYSHAHV GMSGFGASAP AGVLYKKFGI TVEEVVRTGR
660 670
ELAKRFPDGT APLKNSSFSK M
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ427448 Genomic DNA Translation: CAD20572.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ427448 Genomic DNA Translation: CAD20572.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R9J | X-ray | 2.22 | A/B | 1-671 | [»] | |
SMRi | Q8MPM3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
BRENDAi | 2.2.1.1, 2951 |
Miscellaneous databases
EvolutionaryTracei | Q8MPM3 |
PHI-basei | PHI:8028 |
Family and domain databases
CDDi | cd02012, TPP_TK, 1 hit |
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR005478, Transketolase_bac-like IPR020826, Transketolase_BS IPR033248, Transketolase_C IPR033247, Transketolase_fam IPR005474, Transketolase_N |
PANTHERi | PTHR43522, PTHR43522, 1 hit |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit PF00456, Transketolase_N, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
TIGRFAMsi | TIGR00232, tktlase_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00801, TRANSKETOLASE_1, 1 hit PS00802, TRANSKETOLASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q8MPM3_LEIME | |
Accessioni | Q8MPM3Primary (citable) accession number: Q8MPM3 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | October 1, 2002 |
Last sequence update: | October 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 88 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |