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UniProtKB - Q8MP06 (SNO1_TYRJA)
Protein
Senecionine N-oxygenase
Gene
sno1
Organism
Tyria jacobaeae (Cinnabar moth)
Status
Functioni
NADPH-dependent monooxygenase that detoxifies senecionine and similar plant alkaloids that are ingested by the larvae. Is active towards a narrow range of related substrates with highest activity towards senecionine, followed by seneciphylline, retrorsine, monocrotaline, senecivernine, axillarine and axillaridine.
1 PublicationMiscellaneous
Larvae store pyrrolizidine alkaloids from their host plant S.jacobaeae as non-toxic N-oxides. This serves as protection against insectivores; after ingestion of the larvae, the N-oxides are reduced in the gut of the insectivores to form toxic alkaloids (PubMed:11972041).1 Publication
Catalytic activityi
- EC:1.14.13.1012 Publications
Cofactori
FAD2 Publications
Absorptioni
Abs(max)=456 nm1 Publication
Kineticsi
- KM=1.4 µM for senecionine1 Publication
- KM=1.3 µM for NADPH1 Publication
- KM=12.5 µM for monocrotaline1 Publication
pH dependencei
Optimum pH is 7.0.1 Publication
Temperature dependencei
Optimum temperature is 40-45 degrees Celsius.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 32 – 37 | FADSequence analysis | 6 | |
Nucleotide bindingi | 215 – 220 | NADPSequence analysis | 6 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: UniProtKB
- N,N-dimethylaniline monooxygenase activity Source: InterPro
- NADP binding Source: InterPro
- senecionine N-oxygenase activity Source: UniProtKB
GO - Biological processi
- alkaloid metabolic process Source: UniProtKB
- protein homotetramerization Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | FAD, Flavoprotein, NADP |
Enzyme and pathway databases
BRENDAi | 1.14.13.101, 6769 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:sno1 |
Organismi | Tyria jacobaeae (Cinnabar moth) |
Taxonomic identifieri | 179666 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Noctuoidea › Erebidae › Arctiinae › Callimorphini › Tyria |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | 1 PublicationAdd BLAST | 22 | |
ChainiPRO_5000068127 | 23 – 456 | Senecionine N-oxygenaseAdd BLAST | 434 |
Proteomic databases
PRIDEi | Q8MP06 |
Expressioni
Tissue specificityi
Hemolymph.1 Publication
Interactioni
Subunit structurei
Homotetramer.
1 PublicationFamily & Domainsi
Sequence similaritiesi
Belongs to the FMO family.Curated
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR000960, Flavin_mOase IPR020946, Flavin_mOase-like |
Pfami | View protein in Pfam PF00743, FMO-like, 2 hits |
PRINTSi | PR00370, FMOXYGENASE |
SUPFAMi | SSF51905, SSF51905, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8MP06-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFRKFVIMLV LSLLVAAGIS QASSASRVCI IGAGYSGLAT ARYLQDYGLN
60 70 80 90 100
YTIFEATPNI GGTWRYDPRV GTDEDGIPIY SSNYKNLRVN SPVDLMTYHG
110 120 130 140 150
YEFQEGTRSF ISGNCFYKYM KSFVRHFGLM ENIQVRSLVT WVQRTEDKWN
160 170 180 190 200
LTYMKTDTRK NYTEECDFVV VASGEFSTPK IPHIKGQEEY KGKTMHSHDY
210 220 230 240 250
KEAESFRGQR VLVIGAGPSG LDVVMQLSNI TSKLVHSQHI LKSWHIFNQP
260 270 280 290 300
DFPGNFISKP NVKHFTANGA VFEDDTVEEF DMVIYCTGFY YNHPFLSTLS
310 320 330 340 350
SGITATENYV MPLYQQVVNI NQPTMTFVGI CKPFFAKLLD QQAHYSAKLA
360 370 380 390 400
AGHFKLPSQD KMLRHWLEHV QMLREAQFKI TDVNSVGPNV DEYFKALHKE
410 420 430 440 450
AGVPLLPPVY ASVFVFSGKT LLEDLQNYRE YDYRIISDTQ FKKKYNPREE
VCPYDD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ420233 mRNA Translation: CAD12369.1 |
Genome annotation databases
KEGGi | ag:CAD12369 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ420233 mRNA Translation: CAD12369.1 |
3D structure databases
AlphaFoldDBi | Q8MP06 |
SMRi | Q8MP06 |
ModBasei | Search... |
Proteomic databases
PRIDEi | Q8MP06 |
Genome annotation databases
KEGGi | ag:CAD12369 |
Enzyme and pathway databases
BRENDAi | 1.14.13.101, 6769 |
Family and domain databases
Gene3Di | 3.50.50.60, 2 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR000960, Flavin_mOase IPR020946, Flavin_mOase-like |
Pfami | View protein in Pfam PF00743, FMO-like, 2 hits |
PRINTSi | PR00370, FMOXYGENASE |
SUPFAMi | SSF51905, SSF51905, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SNO1_TYRJA | |
Accessioni | Q8MP06Primary (citable) accession number: Q8MP06 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | October 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families