PLAU

Functionⁱ

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityⁱ

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulationⁱ

Inhibited by SERPINA5.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	226	Charge relay systemBy similarity	1
Active siteⁱ	277	Charge relay systemBy similarity	1
Active siteⁱ	378	Charge relay systemBy similarity	1

GO - Molecular functionⁱ

serine-type endopeptidase activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

fibrinolysis Source: Ensembl
plasminogen activation Source: Ensembl
positive regulation of cell migration Source: Ensembl
regulation of cell adhesion mediated by integrin Source: Ensembl
regulation of cell proliferation Source: Ensembl
regulation of signaling receptor activity Source: Ensembl
regulation of smooth muscle cell-matrix adhesion Source: Ensembl
regulation of smooth muscle cell migration Source: Ensembl
response to hypoxia Source: Ensembl
smooth muscle cell migration Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Serine protease
Biological process	Plasminogen activation

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	S01.231

MEROPSⁱ

S01.231

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Urokinase-type plasminogen activator (EC:3.4.21.73) Short name: U-plasminogen activator Short name: uPA Cleaved into the following 3 chains: Urokinase-type plasminogen activator long chain A Urokinase-type plasminogen activator short chain A Urokinase-type plasminogen activator chain B
Gene namesⁱ	Name:PLAU
Organismⁱ	Oryctolagus cuniculus (Rabbit)
Taxonomic identifierⁱ	9986 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus
Proteomesⁱ	UP000001811 Componentⁱ: Chromosome 18

Keywords - Cellular componentⁱ

Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 16	Sequence analysisAdd BLAST	16
ChainⁱPRO_0000285901	17 – 433	Urokinase-type plasminogen activatorAdd BLAST	417
ChainⁱPRO_0000285902	21 – 179	Urokinase-type plasminogen activator long chain ABy similarityAdd BLAST	159
ChainⁱPRO_0000285903	157 – 179	Urokinase-type plasminogen activator short chain ABy similarityAdd BLAST	23
ChainⁱPRO_0000285904	181 – 433	Urokinase-type plasminogen activator chain BBy similarityAdd BLAST	253

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	33 ↔ 41	By similarity
Disulfide bondⁱ	35 ↔ 53	By similarity
Disulfide bondⁱ	55 ↔ 64	By similarity
Disulfide bondⁱ	72 ↔ 153	By similarity
Disulfide bondⁱ	93 ↔ 135	By similarity
Disulfide bondⁱ	124 ↔ 148	By similarity
Disulfide bondⁱ	170 ↔ 301	Interchain (between A and B chains)PROSITE-ProRule annotation
Disulfide bondⁱ	211 ↔ 227	By similarity
Disulfide bondⁱ	219 ↔ 290	By similarity
Disulfide bondⁱ	315 ↔ 384	By similarity
Glycosylationⁱ	324	N-linked (GlcNAc...) asparagineSequence analysis	1
Modified residueⁱ	325	PhosphoserineBy similarity	1
Disulfide bondⁱ	347 ↔ 363	By similarity
Disulfide bondⁱ	374 ↔ 402	By similarity

Post-translational modificationⁱ

Phosphorylation of Ser-325 abolishes proadhesive ability but does not interfere with receptor binding.By similarity

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSOCUG00000003155

Bgeeⁱ

ENSOCUG00000003155

Interactionⁱ

Subunit structureⁱ

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR (By similarity).By similarity

Protein-protein interaction databases

STRINGⁱ	9986.ENSOCUP00000023296

STRINGⁱ

9986.ENSOCUP00000023296

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8MHY7
SMRⁱ	Q8MHY7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	29 – 65	EGF-likePROSITE-ProRule annotationAdd BLAST	37
Domainⁱ	71 – 153	KringlePROSITE-ProRule annotationAdd BLAST	83
Domainⁱ	181 – 426	Peptidase S1PROSITE-ProRule annotationAdd BLAST	246

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	36 – 59	Binds urokinase plasminogen activator surface receptorBy similarityAdd BLAST		24
Regionⁱ	154 – 180	Connecting peptideBy similarityAdd BLAST		27

Sequence similaritiesⁱ

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domainⁱ

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGⁱ	ENOG410IGFI Eukaryota COG5640 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000119133
HOGENOMⁱ	HOG000237314
HOVERGENⁱ	HBG008633
InParanoidⁱ	Q8MHY7
KEGG Orthology (KO) More...KOⁱ	K01348
OMAⁱ	QPWFAAI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0AH5
TreeFamⁱ	TF329901

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00190 Tryp_SPc, 1 hit
Gene3Dⁱ	2.40.20.10, 1 hit
InterProⁱ	View protein in InterPro IPR013032 EGF-like_CS IPR000742 EGF-like_dom IPR000001 Kringle IPR013806 Kringle-like IPR018056 Kringle_CS IPR038178 Kringle_sf IPR009003 Peptidase_S1_PA IPR001314 Peptidase_S1A IPR001254 Trypsin_dom IPR018114 TRYPSIN_HIS IPR033116 TRYPSIN_SER IPR034814 Urokinase
PANTHERⁱ	PTHR24264:SF38 PTHR24264:SF38, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00051 Kringle, 1 hit PF00089 Trypsin, 1 hit
PRINTSⁱ	PR00722 CHYMOTRYPSIN
SMARTⁱ	View protein in SMART SM00130 KR, 1 hit SM00020 Tryp_SPc, 1 hit
SUPFAMⁱ	SSF50494 SSF50494, 1 hit SSF57440 SSF57440, 1 hit
PROSITEⁱ	View protein in PROSITE PS00022 EGF_1, 1 hit PS50026 EGF_3, 1 hit PS00021 KRINGLE_1, 1 hit PS50070 KRINGLE_2, 1 hit PS50240 TRYPSIN_DOM, 1 hit PS00134 TRYPSIN_HIS, 1 hit PS00135 TRYPSIN_SER, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q8MHY7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRVLLVCLLL CALVVSDSEG SHELHGVSDA SNCGCLNGGT CVTYKYFSNI 
        60         70         80         90        100
WRCNCPKKFQ GEHCEIDTLK TCYHGDGHSY RGKANTDIMD RPCLAWNSAN 
       110        120        130        140        150
VLTKTYHAHR PDALQLGLGK HNYCRNPDHQ RRPWCYVQVG LKQLIQECKV 
       160        170        180        190        200
HDCSSGKKPA LPPGKLEFQC GQKALRPRFK IIGGEFTIIE NQPWFAAIYR 
       210        220        230        240        250
RHRGGSVTYV CGGSLISPCW VVSATHCFIN HQKKEDYIVY LGRSRLNSMT 
       260        270        280        290        300
PGEMKFEVEQ LILHEGYRAD TLAHHNDIAL LKILSNNGQC AQPSRSIQTI 
       310        320        330        340        350
CLPPWNADPN FGTSCEITGF GKENSTDYLY PEQLKMTVVK LVSYQECQQP 
       360        370        380        390        400
HYYGSEVTTK MLCAADPQWE TDSCQGDSGG PLVCSVQGRM TLTGIVSWGR 
       410        420        430 
GCALKNKPGV YTRVSRFLPW IRSHIGEENG LAL

Length:433

Mass (Da):48,444

Last modified:October 1, 2002 - v1

Checksum:ⁱ6DD35A371010A6EE

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	268	R → S in AAM83187 (PubMed:12149463).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY029517 mRNA Translation: AAK40239.1 AY122285 mRNA Translation: AAM83187.1 AB087224 mRNA Translation: BAC02685.1 AY453856 Genomic DNA Translation: AAR21232.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001075480.1, NM_001082011.1 XP_008268125.1, XM_008269903.2
UniGeneⁱ	Ocu.2313

Genome annotation databases

Ensemblⁱ	ENSOCUT00000029709; ENSOCUP00000023296; ENSOCUG00000003155
GeneIDⁱ	100008633
KEGGⁱ	ocu:100008633

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q8MHY7	Urokinase-type plasminogen activator	)	HUMAN		431
UPI0005F3B64C		MANLE		435
Plasminogen activator, urokinase		PANTR		431
PLAU isoform 1		PONAB		431
Plasminogen activator, urokinase		PANPA		431
+188

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY029517 mRNA Translation: AAK40239.1 AY122285 mRNA Translation: AAM83187.1 AB087224 mRNA Translation: BAC02685.1 AY453856 Genomic DNA Translation: AAR21232.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001075480.1, NM_001082011.1 XP_008268125.1, XM_008269903.2
UniGeneⁱ	Ocu.2313

3D structure databases

ProteinModelPortalⁱ	Q8MHY7
SMRⁱ	Q8MHY7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	9986.ENSOCUP00000023296

STRINGⁱ

9986.ENSOCUP00000023296

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	S01.231

MEROPSⁱ

S01.231

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSOCUT00000029709; ENSOCUP00000023296; ENSOCUG00000003155
GeneIDⁱ	100008633
KEGGⁱ	ocu:100008633

Organism-specific databases

CTDⁱ	5328

CTDⁱ

5328

Phylogenomic databases

eggNOGⁱ	ENOG410IGFI Eukaryota COG5640 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000119133
HOGENOMⁱ	HOG000237314
HOVERGENⁱ	HBG008633
InParanoidⁱ	Q8MHY7
KEGG Orthology (KO) More...KOⁱ	K01348
OMAⁱ	QPWFAAI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0AH5
TreeFamⁱ	TF329901

Gene expression databases

Bgeeⁱ	ENSOCUG00000003155

Bgeeⁱ

ENSOCUG00000003155

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00190 Tryp_SPc, 1 hit
Gene3Dⁱ	2.40.20.10, 1 hit
InterProⁱ	View protein in InterPro IPR013032 EGF-like_CS IPR000742 EGF-like_dom IPR000001 Kringle IPR013806 Kringle-like IPR018056 Kringle_CS IPR038178 Kringle_sf IPR009003 Peptidase_S1_PA IPR001314 Peptidase_S1A IPR001254 Trypsin_dom IPR018114 TRYPSIN_HIS IPR033116 TRYPSIN_SER IPR034814 Urokinase
PANTHERⁱ	PTHR24264:SF38 PTHR24264:SF38, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00051 Kringle, 1 hit PF00089 Trypsin, 1 hit
PRINTSⁱ	PR00722 CHYMOTRYPSIN
SMARTⁱ	View protein in SMART SM00130 KR, 1 hit SM00020 Tryp_SPc, 1 hit
SUPFAMⁱ	SSF50494 SSF50494, 1 hit SSF57440 SSF57440, 1 hit
PROSITEⁱ	View protein in PROSITE PS00022 EGF_1, 1 hit PS50026 EGF_3, 1 hit PS00021 KRINGLE_1, 1 hit PS50070 KRINGLE_2, 1 hit PS50240 TRYPSIN_DOM, 1 hit PS00134 TRYPSIN_HIS, 1 hit PS00135 TRYPSIN_SER, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UROK_RABIT
Accessionⁱ	Q8MHY7Primary (citable) accession number: Q8MHY7 Secondary accession number(s): Q8MIL0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
	Last sequence update:	October 1, 2002
	Last modified:	May 23, 2018
	This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

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UniProtKB - Q8MHY7 (UROK_RABIT)

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Urokinase-type plasminogen activator

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Enzyme regulationⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ