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Protein

Urokinase-type plasminogen activator

Gene

PLAU

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei226Charge relay systemBy similarity1
Active sitei277Charge relay systemBy similarity1
Active sitei378Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processPlasminogen activation

Protein family/group databases

MEROPSiS01.231

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:PLAU
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Chromosome 18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000028590117 – 433Urokinase-type plasminogen activatorAdd BLAST417
ChainiPRO_000028590221 – 179Urokinase-type plasminogen activator long chain ABy similarityAdd BLAST159
ChainiPRO_0000285903157 – 179Urokinase-type plasminogen activator short chain ABy similarityAdd BLAST23
ChainiPRO_0000285904181 – 433Urokinase-type plasminogen activator chain BBy similarityAdd BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 41By similarity
Disulfide bondi35 ↔ 53By similarity
Disulfide bondi55 ↔ 64By similarity
Disulfide bondi72 ↔ 153By similarity
Disulfide bondi93 ↔ 135By similarity
Disulfide bondi124 ↔ 148By similarity
Disulfide bondi170 ↔ 301Interchain (between A and B chains)PROSITE-ProRule annotation
Disulfide bondi211 ↔ 227By similarity
Disulfide bondi219 ↔ 290By similarity
Disulfide bondi315 ↔ 384By similarity
Glycosylationi324N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei325PhosphoserineBy similarity1
Disulfide bondi347 ↔ 363By similarity
Disulfide bondi374 ↔ 402By similarity

Post-translational modificationi

Phosphorylation of Ser-325 abolishes proadhesive ability but does not interfere with receptor binding.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen

Expressioni

Gene expression databases

BgeeiENSOCUG00000003155

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR (By similarity).By similarity

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000023296

Structurei

3D structure databases

ProteinModelPortaliQ8MHY7
SMRiQ8MHY7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 65EGF-likePROSITE-ProRule annotationAdd BLAST37
Domaini71 – 153KringlePROSITE-ProRule annotationAdd BLAST83
Domaini181 – 426Peptidase S1PROSITE-ProRule annotationAdd BLAST246

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 59Binds urokinase plasminogen activator surface receptorBy similarityAdd BLAST24
Regioni154 – 180Connecting peptideBy similarityAdd BLAST27

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiENOG410IGFI Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000119133
HOGENOMiHOG000237314
HOVERGENiHBG008633
InParanoidiQ8MHY7
KOiK01348
OMAiQPWFAAI
OrthoDBiEOG091G0AH5
TreeFamiTF329901

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
IPR034814 Urokinase
PANTHERiPTHR24264:SF38 PTHR24264:SF38, 1 hit
PfamiView protein in Pfam
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8MHY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLLVCLLL CALVVSDSEG SHELHGVSDA SNCGCLNGGT CVTYKYFSNI
60 70 80 90 100
WRCNCPKKFQ GEHCEIDTLK TCYHGDGHSY RGKANTDIMD RPCLAWNSAN
110 120 130 140 150
VLTKTYHAHR PDALQLGLGK HNYCRNPDHQ RRPWCYVQVG LKQLIQECKV
160 170 180 190 200
HDCSSGKKPA LPPGKLEFQC GQKALRPRFK IIGGEFTIIE NQPWFAAIYR
210 220 230 240 250
RHRGGSVTYV CGGSLISPCW VVSATHCFIN HQKKEDYIVY LGRSRLNSMT
260 270 280 290 300
PGEMKFEVEQ LILHEGYRAD TLAHHNDIAL LKILSNNGQC AQPSRSIQTI
310 320 330 340 350
CLPPWNADPN FGTSCEITGF GKENSTDYLY PEQLKMTVVK LVSYQECQQP
360 370 380 390 400
HYYGSEVTTK MLCAADPQWE TDSCQGDSGG PLVCSVQGRM TLTGIVSWGR
410 420 430
GCALKNKPGV YTRVSRFLPW IRSHIGEENG LAL
Length:433
Mass (Da):48,444
Last modified:October 1, 2002 - v1
Checksum:i6DD35A371010A6EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti268R → S in AAM83187 (PubMed:12149463).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029517 mRNA Translation: AAK40239.1
AY122285 mRNA Translation: AAM83187.1
AB087224 mRNA Translation: BAC02685.1
AY453856 Genomic DNA Translation: AAR21232.1
RefSeqiNP_001075480.1, NM_001082011.1
XP_008268125.1, XM_008269903.2
UniGeneiOcu.2313

Genome annotation databases

EnsembliENSOCUT00000029709; ENSOCUP00000023296; ENSOCUG00000003155
GeneIDi100008633
KEGGiocu:100008633

Similar proteinsi

Entry informationi

Entry nameiUROK_RABIT
AccessioniPrimary (citable) accession number: Q8MHY7
Secondary accession number(s): Q8MIL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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