UniProtKB - Q8MHY7 (UROK_RABIT)
Urokinase-type plasminogen activator
PLAU
Functioni
Catalytic activityi
- Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin. EC:3.4.21.73
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 226 | Charge relay systemBy similarity | 1 | |
Active sitei | 277 | Charge relay systemBy similarity | 1 | |
Active sitei | 378 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: UniProtKB-EC
GO - Biological processi
- fibrinolysis Source: Ensembl
- plasminogen activation Source: Ensembl
- positive regulation of cell migration Source: Ensembl
- regulation of cell adhesion mediated by integrin Source: Ensembl
- regulation of cell population proliferation Source: Ensembl
- regulation of signaling receptor activity Source: Ensembl
- regulation of smooth muscle cell-matrix adhesion Source: Ensembl
- regulation of smooth muscle cell migration Source: Ensembl
- response to hypoxia Source: Ensembl
- smooth muscle cell migration Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Plasminogen activation |
Protein family/group databases
MEROPSi | S01.231 |
Names & Taxonomyi
Protein namesi | Recommended name: Urokinase-type plasminogen activator (EC:3.4.21.73)Short name: U-plasminogen activator Short name: uPA Cleaved into the following 3 chains: |
Gene namesi | Name:PLAU |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: Ensembl
Other locations
- cell surface Source: Ensembl
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | Sequence analysisAdd BLAST | 16 | |
ChainiPRO_0000285901 | 17 – 433 | Urokinase-type plasminogen activatorAdd BLAST | 417 | |
ChainiPRO_0000285902 | 21 – 179 | Urokinase-type plasminogen activator long chain ABy similarityAdd BLAST | 159 | |
ChainiPRO_0000285903 | 157 – 179 | Urokinase-type plasminogen activator short chain ABy similarityAdd BLAST | 23 | |
ChainiPRO_0000285904 | 181 – 433 | Urokinase-type plasminogen activator chain BBy similarityAdd BLAST | 253 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 33 ↔ 41 | By similarity | ||
Disulfide bondi | 35 ↔ 53 | By similarity | ||
Disulfide bondi | 55 ↔ 64 | By similarity | ||
Disulfide bondi | 72 ↔ 153 | By similarity | ||
Disulfide bondi | 93 ↔ 135 | By similarity | ||
Disulfide bondi | 124 ↔ 148 | By similarity | ||
Disulfide bondi | 170 ↔ 301 | Interchain (between A and B chains)PROSITE-ProRule annotation | ||
Disulfide bondi | 211 ↔ 227 | By similarity | ||
Disulfide bondi | 219 ↔ 290 | By similarity | ||
Disulfide bondi | 315 ↔ 384 | By similarity | ||
Glycosylationi | 324 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 325 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 347 ↔ 363 | By similarity | ||
Disulfide bondi | 374 ↔ 402 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Phosphoprotein, ZymogenExpressioni
Gene expression databases
Bgeei | ENSOCUG00000003155, Expressed in adult mammalian kidney and 15 other tissues |
Interactioni
Subunit structurei
Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation.
Interacts with MRC2.
Interacts with PLAUR. In complex with SERPINE1, interacts with PLAUR/uPAR.
Interacts with SORL1 and LRP1, either alone or in complex with SERPINE1; these interactions are abolished in the presence of LRPAP1/RAP. The ternary complex composed of PLAUR-PLAU-PAI1 also interacts with SORLA.
By similarityProtein-protein interaction databases
STRINGi | 9986.ENSOCUP00000023296 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 65 | EGF-likePROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 71 – 153 | KringlePROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 181 – 426 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 246 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 36 – 59 | Binds urokinase plasminogen activator surface receptorBy similarityAdd BLAST | 24 | |
Regioni | 154 – 180 | Connecting peptideBy similarityAdd BLAST | 27 |
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Kringle, SignalPhylogenomic databases
eggNOGi | ENOG502QRMI, Eukaryota |
GeneTreei | ENSGT00990000203596 |
HOGENOMi | CLU_006842_18_4_1 |
InParanoidi | Q8MHY7 |
OMAi | MKFEVEK |
OrthoDBi | 1314811at2759 |
TreeFami | TF329901 |
Family and domain databases
CDDi | cd00108, KR, 1 hit cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR000742, EGF-like_dom IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER IPR034814, Urokinase |
PANTHERi | PTHR24264:SF38, PTHR24264:SF38, 1 hit |
Pfami | View protein in Pfam PF00051, Kringle, 1 hit PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00130, KR, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS50026, EGF_3, 1 hit PS00021, KRINGLE_1, 1 hit PS50070, KRINGLE_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MRVLLVCLLL CALVVSDSEG SHELHGVSDA SNCGCLNGGT CVTYKYFSNI
60 70 80 90 100
WRCNCPKKFQ GEHCEIDTLK TCYHGDGHSY RGKANTDIMD RPCLAWNSAN
110 120 130 140 150
VLTKTYHAHR PDALQLGLGK HNYCRNPDHQ RRPWCYVQVG LKQLIQECKV
160 170 180 190 200
HDCSSGKKPA LPPGKLEFQC GQKALRPRFK IIGGEFTIIE NQPWFAAIYR
210 220 230 240 250
RHRGGSVTYV CGGSLISPCW VVSATHCFIN HQKKEDYIVY LGRSRLNSMT
260 270 280 290 300
PGEMKFEVEQ LILHEGYRAD TLAHHNDIAL LKILSNNGQC AQPSRSIQTI
310 320 330 340 350
CLPPWNADPN FGTSCEITGF GKENSTDYLY PEQLKMTVVK LVSYQECQQP
360 370 380 390 400
HYYGSEVTTK MLCAADPQWE TDSCQGDSGG PLVCSVQGRM TLTGIVSWGR
410 420 430
GCALKNKPGV YTRVSRFLPW IRSHIGEENG LAL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A5F9D794 | A0A5F9D794_RABIT | Urokinase-type plasminogen activato... | PLAU | 428 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 268 | R → S in AAM83187 (PubMed:12149463).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY029517 mRNA Translation: AAK40239.1 AY122285 mRNA Translation: AAM83187.1 AB087224 mRNA Translation: BAC02685.1 AY453856 Genomic DNA Translation: AAR21232.1 |
RefSeqi | NP_001075480.1, NM_001082011.1 XP_008268125.1, XM_008269903.2 |
Genome annotation databases
Ensembli | ENSOCUT00000029709; ENSOCUP00000023296; ENSOCUG00000003155 |
GeneIDi | 100008633 |
KEGGi | ocu:100008633 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY029517 mRNA Translation: AAK40239.1 AY122285 mRNA Translation: AAM83187.1 AB087224 mRNA Translation: BAC02685.1 AY453856 Genomic DNA Translation: AAR21232.1 |
RefSeqi | NP_001075480.1, NM_001082011.1 XP_008268125.1, XM_008269903.2 |
3D structure databases
SMRi | Q8MHY7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000023296 |
Protein family/group databases
MEROPSi | S01.231 |
Protocols and materials databases
ABCDi | Q8MHY7, 1 sequenced antibody |
Genome annotation databases
Ensembli | ENSOCUT00000029709; ENSOCUP00000023296; ENSOCUG00000003155 |
GeneIDi | 100008633 |
KEGGi | ocu:100008633 |
Organism-specific databases
CTDi | 5328 |
Phylogenomic databases
eggNOGi | ENOG502QRMI, Eukaryota |
GeneTreei | ENSGT00990000203596 |
HOGENOMi | CLU_006842_18_4_1 |
InParanoidi | Q8MHY7 |
OMAi | MKFEVEK |
OrthoDBi | 1314811at2759 |
TreeFami | TF329901 |
Gene expression databases
Bgeei | ENSOCUG00000003155, Expressed in adult mammalian kidney and 15 other tissues |
Family and domain databases
CDDi | cd00108, KR, 1 hit cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR000742, EGF-like_dom IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER IPR034814, Urokinase |
PANTHERi | PTHR24264:SF38, PTHR24264:SF38, 1 hit |
Pfami | View protein in Pfam PF00051, Kringle, 1 hit PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00130, KR, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS50026, EGF_3, 1 hit PS00021, KRINGLE_1, 1 hit PS50070, KRINGLE_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UROK_RABIT | |
Accessioni | Q8MHY7Primary (citable) accession number: Q8MHY7 Secondary accession number(s): Q8MIL0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | October 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries