Maturation protein A2

Functionⁱ

Induces host cell lysis (PubMed:11892805, PubMed:29078304).

Inhibits host MurA activity thereby blocking the synthesis of murein precursors necessary for the host cell wall biosynthesis (PubMed:11423662, PubMed:29078304).

May be responsible for the attachment to the host pilus. Makes extensive contacts with the viral genome (PubMed:28111107).

5 Publications

GO - Biological processⁱ

adhesion receptor-mediated virion attachment to host cell Source: UniProtKB-KW
cytolysis Source: UniProtKB-KW
suppression by virus of host cell wall biogenesis
Source: UniProtKB
Inferred from direct assayⁱ
- Ref.7
  "A protein antibiotic in the phage Qbeta virion: diversity in lysis targets."
  Bernhardt T.G., Wang I.N., Struck D.K., Young R.
  Science 292:2326-2329(2001) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION.
- Ref.12
  "Structures of Qbeta virions, virus-like particles, and the Qbeta-MurA complex reveal internal coat proteins and the mechanism of host lysis."
  Cui Z., Gorzelnik K.V., Chang J.Y., Langlais C., Jakana J., Young R., Zhang J.
  Proc. Natl. Acad. Sci. U.S.A. 114:11697-11702(2017) [PubMed] [Europe PMC] [Abstract]
  Cited for: STRUCTURE BY ELECTRON MICROSCOPY (4.40 ANGSTROMS), INTERACTION WITH HOST MURA, INTERACTION WITH THE CAPSID PROTEIN, FUNCTION, SUBCELLULAR LOCATION.
suppression by virus of host peptidoglycan biosynthetic process
Source: CACAO
Inferred from direct assayⁱ
- Ref.7
  "A protein antibiotic in the phage Qbeta virion: diversity in lysis targets."
  Bernhardt T.G., Wang I.N., Struck D.K., Young R.
  Science 292:2326-2329(2001) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION.
viral entry into host cell Source: UniProtKB-KW
viral release by cytolysis via suppression of host peptidoglycan biosynthetic process
Source: CACAO
Inferred from direct assayⁱ
- Ref.7
  "A protein antibiotic in the phage Qbeta virion: diversity in lysis targets."
  Bernhardt T.G., Wang I.N., Struck D.K., Young R.
  Science 292:2326-2329(2001) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION.
viral release from host cell by cytolysis
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- Ref.6
  "The lysis function of RNA bacteriophage Qbeta is mediated by the maturation (A2) protein."
  Karnik S., Billeter M.
  EMBO J. 2:1521-1526(1983) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION.
virion attachment to host cell pilus Source: UniProtKB-KW

Complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cytolysis, Host cell lysis by virus, Host-virus interaction, Viral attachment to host adhesion receptor, Viral attachment to host cell, Viral attachment to host cell pilus, Viral release from host cell, Virus entry into host cell

Biological process

Cytolysis, Host cell lysis by virus, Host-virus interaction, Viral attachment to host adhesion receptor, Viral attachment to host cell, Viral attachment to host cell pilus, Viral release from host cell, Virus entry into host cell

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Maturation protein A2Imported Short name: MP Alternative name(s): A2 protein
Organismⁱ	Escherichia virus Qbeta (Bacteriophage Q-beta)Imported
Taxonomic identifierⁱ	39803 [NCBI]
Taxonomic lineageⁱ	Viruses › Riboviria › Orthornavirae › Lenarviricota › Allassoviricetes › Levivirales › Leviviridae › Allolevivirus
Virus hostⁱ	Escherichia coli [TaxID: 562]
Proteomesⁱ	UP000305125 Componentⁱ: Genome UP000306921 Componentⁱ: Genome UP000185268 Componentⁱ: Genome UP000309733 Componentⁱ: Genome UP000001616 Componentⁱ: Genome

Subcellular locationⁱ

Virion
2 Publications
Manual assertion based on experiment inⁱ
- Ref.10
  "Asymmetric cryo-EM structure of the canonical Allolevivirus Qbeta reveals a single maturation protein and the genomic ssRNA in situ."
  Gorzelnik K.V., Cui Z., Reed C.A., Jakana J., Young R., Zhang J.
  Proc. Natl. Acad. Sci. U.S.A. 113:11519-11524(2016) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION.
- Ref.12
  "Structures of Qbeta virions, virus-like particles, and the Qbeta-MurA complex reveal internal coat proteins and the mechanism of host lysis."
  Cui Z., Gorzelnik K.V., Chang J.Y., Langlais C., Jakana J., Young R., Zhang J.
  Proc. Natl. Acad. Sci. U.S.A. 114:11697-11702(2017) [PubMed] [Europe PMC] [Abstract]
  Cited for: STRUCTURE BY ELECTRON MICROSCOPY (4.40 ANGSTROMS), INTERACTION WITH HOST MURA, INTERACTION WITH THE CAPSID PROTEIN, FUNCTION, SUBCELLULAR LOCATION.

Note:

A single copy of the maturation protein is present in the virion.2 Publications

Keywords - Cellular componentⁱ

Virion

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000438881	1 – 420	Maturation protein A2Add BLAST		420

Interactionⁱ

Subunit structureⁱ

Interacts with host MurA; this interaction inhibits the first step in host cell wall synthesis (PubMed:22934834, PubMed:29078304).

Interacts with the capsid protein (PubMed:28111107, PubMed:29078304).

3 Publications

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	6 – 10	Combined sources	5
Beta strandⁱ	16 – 18	Combined sources	3
Helixⁱ	20 – 24	Combined sources	5
Beta strandⁱ	29 – 31	Combined sources	3
Beta strandⁱ	38 – 47	Combined sources	10
Beta strandⁱ	49 – 55	Combined sources	7
Beta strandⁱ	62 – 64	Combined sources	3
Beta strandⁱ	84 – 103	Combined sources	20
Turnⁱ	104 – 106	Combined sources	3
Beta strandⁱ	107 – 120	Combined sources	14
Helixⁱ	129 – 177	Combined sources	49
Helixⁱ	180 – 188	Combined sources	9
Helixⁱ	203 – 210	Combined sources	8
Helixⁱ	212 – 234	Combined sources	23
Beta strandⁱ	237 – 253	Combined sources	17
Beta strandⁱ	261 – 280	Combined sources	20
Turnⁱ	281 – 286	Combined sources	6
Turnⁱ	289 – 291	Combined sources	3
Helixⁱ	295 – 303	Combined sources	9
Turnⁱ	304 – 306	Combined sources	3
Helixⁱ	312 – 315	Combined sources	4
Helixⁱ	317 – 336	Combined sources	20
Helixⁱ	337 – 339	Combined sources	3
Beta strandⁱ	341 – 364	Combined sources	24
Beta strandⁱ	367 – 379	Combined sources	13
Beta strandⁱ	382 – 386	Combined sources	5
Helixⁱ	397 – 400	Combined sources	4
Helixⁱ	402 – 416	Combined sources	15

Show more details

3D structure databases

SMRⁱ	Q8LTE2
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	158 – 176	RNA-binding1 PublicationAdd BLAST	19
Regionⁱ	226 – 236	RNA-binding1 PublicationAdd BLAST	11
Regionⁱ	294 – 298	RNA-binding1 Publication	5

Family and domain databases

InterProⁱ	View protein in InterPro IPR005563, A_protein
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03863, Phage_mat-A, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q8LTE2-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPKLPRGLRF GADNEILNDF QELWFPDLFI ESSDTHPWYT LKGRVLNAHL 
        60         70         80         90        100
DDRLPNVGGR QVRRTPHRVT VPIASSGLRP VTTVQYDPAA LSFLLNARVD 
       110        120        130        140        150
WDFGNGDSAN LVINDFLFRT FAPKEFDFSN SLVPRYTQAF SAFNAKYGTM 
       160        170        180        190        200
IGEGLETIKY LGLLLRRLRE GYRAVKRGDL RALRRVIQSY HNGKWKPATA 
       210        220        230        240        250
GNLWLEFRYG LMPLFYDIRD VMLDWQNRHD KIQRLLRFSV GHGEDYVVEF 
       260        270        280        290        300
DNLYPAVAYF KLKGEITLER RHRHGISYAN REGYAVFDNG SLRPVSDWKE 
       310        320        330        340        350
LATAFINPHE VAWELTPYSF VVDWFLNVGD ILAQQGQLYH NIDIVDGFDR 
       360        370        380        390        400
RDIRLKSFTI KGERNGRPVN VSASLSAVDL FYSRLHTSNL PFATLDLDTT 
       410        420
FSSFKHVLDS IFLLTQRVKR

Length:420

Mass (Da):48,547

Last modified:October 1, 2002 - v1

Checksum:ⁱAF119F7AC04FF950

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱ	29	F → L in strain: Qbeta_2_FR. 1 Publication	1
Natural variantⁱ	76	S → L in strain: Qbeta_2. 1 Publication	1
Natural variantⁱ	100	D → G in strain: Qbeta_2. 1 Publication	1
Natural variantⁱ	110	N → D in strain: Qbeta_2_FR. 1 Publication	1
Natural variantⁱ	121	F → S in strain: TW18. 1 Publication	1
Natural variantⁱ	138	Q → K in strain: Qbeta_3_FR. 1 Publication	1
Natural variantⁱ	189	S → F in strain: Qbeta_3. 1 Publication	1
Natural variantⁱ	198	A → T in strain: TW18. 1 Publication	1
Natural variantⁱ	218	I → V in strain: Qbeta_3_FR. 1 Publication	1
Natural variantⁱ	219	R → G in strain: Qbeta_1. 1 Publication	1
Natural variantⁱ	219	R → K in strain: TW18. 1 Publication	1
Natural variantⁱ	246	Y → C in strain: TW18. 1 Publication	1
Natural variantⁱ	254	Y → C in strain: Qbeta_3. 1 Publication	1
Natural variantⁱ	282	E → G in strain: Qbeta_3_FR and Qbeta_3. 2 Publications	1
Natural variantⁱ	331	I → M in strain: Qbeta_2. 1 Publication	1
Natural variantⁱ	360	I → V in strain: Qbeta_3_F and Qbeta_3. 2 Publications	1
Natural variantⁱ	374	S → D in strain: TW18. 1 Publication	1
Natural variantⁱ	389	N → S in strain: TW18. 1 Publication	1
Natural variantⁱ	404	F → Y in strain: TW18. 1 Publication	1
Natural variantⁱ	412	F → S in strain: Qbeta_2_FR and Qbeta_3FR. 2 Publications	1
Natural variantⁱ	418	V → I in strain: TW18. 1 Publication	1
Natural variantⁱ	419	K → N in strain: Qbeta_2_FR. 1 Publication	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY099114 Genomic RNA Translation: AAM33125.1 FJ483840 Genomic RNA Translation: ACT66729.1 GQ153928 Genomic RNA Translation: ACY07222.1 GQ153929 Genomic RNA Translation: ACY07226.1 GQ153930 Genomic RNA Translation: ACY07230.1 GQ153931 Genomic RNA Translation: ACY07234.1 JF719735 Genomic RNA Translation: AEQ25540.1 JF719736 Genomic RNA Translation: AEQ25544.1 JF719737 Genomic RNA Translation: AEQ25548.1 AB971354 Genomic RNA Translation: BAP18762.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AY099114 Genomic RNA Translation: AAM33125.1
FJ483840 Genomic RNA Translation: ACT66729.1
GQ153928 Genomic RNA Translation: ACY07222.1
GQ153929 Genomic RNA Translation: ACY07226.1
GQ153930 Genomic RNA Translation: ACY07230.1
GQ153931 Genomic RNA Translation: ACY07234.1
JF719735 Genomic RNA Translation: AEQ25540.1
JF719736 Genomic RNA Translation: AEQ25544.1
JF719737 Genomic RNA Translation: AEQ25548.1
AB971354 Genomic RNA Translation: BAP18762.1

Protein	Similar proteins		Species	Score	Length	Source
Q8LTE2	Assembly/maturation protein		BPQBE		420	UniRef90_Q8LTE2
Assembly protein (Fragment)		BPVK		222
Assembly protein (Fragment)		BPQBE		222
Assembly/maturation protein		BPQBE		420
Maturation protein (Fragment)		BPQBE		189
+18

Protein	Similar proteins		Species	Score	Length	Source
Q8LTE2	Maturation protein A2		BPMX1		421	UniRef50_Q8LTE2
Assembly/maturation protein		BPQBE		420
Assembly protein (Fragment)		BPVK		222
A-protein		Enterobacteria phage M11		421
Maturation protein (Fragment)		BPQBE		189
+20

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY099114 Genomic RNA Translation: AAM33125.1 FJ483840 Genomic RNA Translation: ACT66729.1 GQ153928 Genomic RNA Translation: ACY07222.1 GQ153929 Genomic RNA Translation: ACY07226.1 GQ153930 Genomic RNA Translation: ACY07230.1 GQ153931 Genomic RNA Translation: ACY07234.1 JF719735 Genomic RNA Translation: AEQ25540.1 JF719736 Genomic RNA Translation: AEQ25544.1 JF719737 Genomic RNA Translation: AEQ25548.1 AB971354 Genomic RNA Translation: BAP18762.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

AY099114 Genomic RNA Translation: AAM33125.1
FJ483840 Genomic RNA Translation: ACT66729.1
GQ153928 Genomic RNA Translation: ACY07222.1
GQ153929 Genomic RNA Translation: ACY07226.1
GQ153930 Genomic RNA Translation: ACY07230.1
GQ153931 Genomic RNA Translation: ACY07234.1
JF719735 Genomic RNA Translation: AEQ25540.1
JF719736 Genomic RNA Translation: AEQ25544.1
JF719737 Genomic RNA Translation: AEQ25548.1
AB971354 Genomic RNA Translation: BAP18762.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5MNT	X-ray	3.32	A/B/C/D	2-420	[»]
	5VLZ	electron microscopy	4.40	EJ	1-420	[»]
	5VM7	electron microscopy	5.70	A	1-420	[»]
	7LHD	electron microscopy	4.60	M	1-420	[»]
SMRⁱ	Q8LTE2
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Family and domain databases

InterProⁱ	View protein in InterPro IPR005563, A_protein
Pfamⁱ	View protein in Pfam PF03863, Phage_mat-A, 1 hit
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MATA2_BPQBE
Accessionⁱ	Q8LTE2Primary (citable) accession number: Q8LTE2 Secondary accession number(s): C8YJH8 G4WZR5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 15, 2017
	Last sequence update:	October 1, 2002
	Last modified:	May 25, 2022
	This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB - Q8LTE2 (MATA2_BPQBE)

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Functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Molecule processing

Interactionⁱ

Subunit structureⁱ

Structureⁱ

Secondary structure

3D structure databases

Family & Domainsⁱ

Region

Family and domain databases

Sequenceⁱ

Natural variant

Sequence databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

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Supporting data

UniProtKB - Q8LTE2 (MATA2_BPQBE)

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Maturation protein A2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Family and domain databases

Natural variant

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ