UniProtKB - Q8LPS1 (LACS6_ARATH)
Long chain acyl-CoA synthetase 6, peroxisomal
LACS6
Functioni
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation (Probable). Preferentially uses palmitate, palmitoleate, oleate, linoleate and eicosenoate as substrates (PubMed:12177484, PubMed:12366803).
Can use myristate and linolenate as substrates (PubMed:12366803).
May play a regulatory role both in fatty acid import into glyoxysomes and in fatty acid beta-oxidation (PubMed:12481085).
Functions redundantly with LACS7 in lipid mobilization for beta-oxidation during seed germination, which is essential for postgerminative growth and seedling establishment (PubMed:14742880, PubMed:16844736).
2 Publications5 PublicationsCatalytic activityi
- EC:6.2.1.32 PublicationsThis reaction proceeds in the forward2 Publications direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z,12Z,15Z)-octadecatrienoate + ATP + CoA = (9Z,12Z,15Z)-octadecatrienoyl-CoA + AMP + diphosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.CuratedView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 266 – 277 | ATPSequence analysisAdd BLAST | 12 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- long-chain fatty acid-CoA ligase activity Source: UniProtKB
GO - Biological processi
- fatty acid metabolic process Source: UniProtKB
- response to ozone Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, Ligase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT3G05970-MONOMER |
UniPathwayi | UPA00199 |
Chemistry databases
SwissLipidsi | SLP:000001941 |
Names & Taxonomyi
Protein namesi | Recommended name: Long chain acyl-CoA synthetase 6, peroxisomal1 Publication (EC:6.2.1.32 Publications)Short name: AtLACS61 Publication |
Gene namesi | Name:LACS61 Publication Ordered Locus Names:At3g05970Imported ORF Names:F2O10.7Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G05970 |
TAIRi | locus:2083013, AT3G05970 |
Subcellular locationi
Peroxisome
- Peroxisome 2 Publications
- Glyoxysome membrane 1 Publication; Peripheral membrane protein Curated
Cytosol
- cytosol Source: TAIR
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Peroxisome
- glyoxysomal membrane Source: UniProtKB-SubCell
- peroxisome Source: UniProtKB
Other locations
- membrane Source: GO_Central
Keywords - Cellular componenti
Glyoxysome, Membrane, PeroxisomePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000401414 | 1 – 38 | Removed in mature form1 PublicationAdd BLAST | 38 | |
ChainiPRO_0000401415 | 39 – 701 | Long chain acyl-CoA synthetase 6, peroxisomalAdd BLAST | 663 |
Proteomic databases
PaxDbi | Q8LPS1 |
PRIDEi | Q8LPS1 |
ProteomicsDBi | 238554 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
ExpressionAtlasi | Q8LPS1, baseline and differential |
Genevisiblei | Q8LPS1, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 5102, 2 interactors |
STRINGi | 3702.AT3G05970.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 526 – 550 | Fatty acid-bindingSequence analysisAdd BLAST | 25 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 15 – 23 | Microbody targeting signal1 Publication | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1256, Eukaryota |
HOGENOMi | CLU_000022_45_4_1 |
InParanoidi | Q8LPS1 |
OMAi | NAAQHPD |
OrthoDBi | 630541at2759 |
PhylomeDBi | Q8LPS1 |
Family and domain databases
CDDi | cd05927, LC-FACS_euk, 1 hit |
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR045311, LC-FACS_euk |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MDSSSSSSSA AARRRINAIH SHLVTSSRSS PLLRSNPTAG EFCLDNGYSV
60 70 80 90 100
VLPEKLNTGS WNVYRSAKSP FKLVSRFPDH PDIATLHDNF EHAVHDFRDY
110 120 130 140 150
KYLGTRVRVD GTVGDYKWMT YGEAGTARTA LGSGLVHHGI PMGSSVGIYF
160 170 180 190 200
INRPEWLIVD HACSSYSYVS VPLYDTLGPD AVKFIVNHAT VQAIFCVAET
210 220 230 240 250
LNSLLSCLSE MPSVRLVVVV GGLIESLPSL PSSSGVKVVS YSVLLNQGRS
260 270 280 290 300
NPQRFFPPKP DDVATICYTS GTTGTPKGVV LTHANLIANV AGSSFSVKFF
310 320 330 340 350
SSDVYISYLP LAHIYERANQ ILTVYFGVAV GFYQGDNMKL LDDLAALRPT
360 370 380 390 400
VFSSVPRLYN RIYAGIINAV KTSGGLKERL FNAAYNAKKQ ALLNGKSASP
410 420 430 440 450
IWDRLVFNKI KDRLGGRVRF MTSGASPLSP EVMEFLKVCF GGRVTEGYGM
460 470 480 490 500
TETSCVISGM DEGDNLTGHV GSPNPACEVK LVDVPEMNYT SADQPHPRGE
510 520 530 540 550
ICVRGPIIFT GYYKDEIQTK EVIDEDGWLH TGDIGLWLPG GRLKIIDRKK
560 570 580 590 600
NIFKLAQGEY IAPEKIENVY AKCKFVGQCF IYGDSFNSSL VAVVSVDPDV
610 620 630 640 650
LKSWAASEGI KGGDLRELCN NPRVKAAVLS DMDTVGREAQ LRGFEFAKAV
660 670 680 690 700
TLVLEPFTLE NGLLTPTFKI KRPQAKEYFA EAITNMYKEL GASDPSANRG
L
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 60 | S → N in BAB40450 (PubMed:12481085).Curated | 1 | |
Sequence conflicti | 139 | G → V in AAM28873 (PubMed:12177484).Curated | 1 | |
Sequence conflicti | 224 | I → N in BAB40450 (PubMed:12481085).Curated | 1 | |
Sequence conflicti | 479 | V → I in AAM28873 (PubMed:12177484).Curated | 1 | |
Sequence conflicti | 517 | I → V in BAB40450 (PubMed:12481085).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF503756 mRNA Translation: AAM28873.1 AB030317 mRNA Translation: BAB40450.1 AC013454 Genomic DNA Translation: AAF23219.1 Sequence problems. CP002686 Genomic DNA Translation: AEE74324.1 AY094418 mRNA Translation: AAM19792.1 BT001117 mRNA Translation: AAN64508.1 |
RefSeqi | NP_566265.1, NM_111471.3 |
Genome annotation databases
EnsemblPlantsi | AT3G05970.1; AT3G05970.1; AT3G05970 |
GeneIDi | 819767 |
Gramenei | AT3G05970.1; AT3G05970.1; AT3G05970 |
KEGGi | ath:AT3G05970 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF503756 mRNA Translation: AAM28873.1 AB030317 mRNA Translation: BAB40450.1 AC013454 Genomic DNA Translation: AAF23219.1 Sequence problems. CP002686 Genomic DNA Translation: AEE74324.1 AY094418 mRNA Translation: AAM19792.1 BT001117 mRNA Translation: AAN64508.1 |
RefSeqi | NP_566265.1, NM_111471.3 |
3D structure databases
SMRi | Q8LPS1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 5102, 2 interactors |
STRINGi | 3702.AT3G05970.1 |
Chemistry databases
SwissLipidsi | SLP:000001941 |
Proteomic databases
PaxDbi | Q8LPS1 |
PRIDEi | Q8LPS1 |
ProteomicsDBi | 238554 |
Genome annotation databases
EnsemblPlantsi | AT3G05970.1; AT3G05970.1; AT3G05970 |
GeneIDi | 819767 |
Gramenei | AT3G05970.1; AT3G05970.1; AT3G05970 |
KEGGi | ath:AT3G05970 |
Organism-specific databases
Araporti | AT3G05970 |
TAIRi | locus:2083013, AT3G05970 |
Phylogenomic databases
eggNOGi | KOG1256, Eukaryota |
HOGENOMi | CLU_000022_45_4_1 |
InParanoidi | Q8LPS1 |
OMAi | NAAQHPD |
OrthoDBi | 630541at2759 |
PhylomeDBi | Q8LPS1 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
BioCyci | ARA:AT3G05970-MONOMER |
Miscellaneous databases
PROi | PR:Q8LPS1 |
Gene expression databases
ExpressionAtlasi | Q8LPS1, baseline and differential |
Genevisiblei | Q8LPS1, AT |
Family and domain databases
CDDi | cd05927, LC-FACS_euk, 1 hit |
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR045311, LC-FACS_euk |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LACS6_ARATH | |
Accessioni | Q8LPS1Primary (citable) accession number: Q8LPS1 Secondary accession number(s): Q8LKS6, Q9C5U7, Q9SFG1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 30, 2010 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families