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UniProtKB - Q8LMR2 (DCL1_ORYSJ)
Protein
Endoribonuclease Dicer homolog 1
Gene
DCL1
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Involved in the RNA silencing pathway. Cleaves double-stranded RNA to produce microRNAs (miRNAs) of 21-24 nucleotides which target the selective destruction of complementary RNAs. Regulates by this way the development of the plant. May not be involved in small interfering RNAs (siRNAs) production.
2 PublicationsCofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1576 | MagnesiumBy similarity | 1 | |
Sitei | 1668 | Important for activityBy similarity | 1 | |
Metal bindingi | 1672 | MagnesiumBy similarity | 1 | |
Metal bindingi | 1675 | MagnesiumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 287 – 294 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- helicase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- ribonuclease III activity Source: GO_Central
- RNA binding Source: GO_Central
GO - Biological processi
- production of miRNAs involved in gene silencing by miRNA Source: UniProtKB
- production of siRNA involved in RNA interference Source: GO_Central
Keywordsi
Molecular function | Endonuclease, Helicase, Hydrolase, Nuclease, RNA-binding |
Biological process | RNA-mediated gene silencing |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Endoribonuclease Dicer homolog 1Alternative name(s): |
Gene namesi | Name:DCL1 Ordered Locus Names:Os03g0121800, LOC_Os03g02970 ORF Names:OJ1705B08.11, OsJ_09217 |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Severe dwarfism and dark green color. Seedling viability compromised.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000378416 | 1 – 1883 | Endoribonuclease Dicer homolog 1Add BLAST | 1883 |
Proteomic databases
PaxDbi | Q8LMR2 |
Interactioni
Subunit structurei
May interact with ARGONAUTE1 or PINHEAD through their common PAZ domains.
By similarityProtein-protein interaction databases
STRINGi | 4530.OS03T0121901-00 |
Structurei
3D structure databases
AlphaFoldDBi | Q8LMR2 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 274 – 413 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 140 | |
Domaini | 629 – 789 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 161 | |
Domaini | 817 – 912 | Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 1156 – 1296 | PAZPROSITE-ProRule annotationAdd BLAST | 141 | |
Domaini | 1320 – 1498 | RNase III 1PROSITE-ProRule annotationAdd BLAST | 179 | |
Domaini | 1538 – 1686 | RNase III 2PROSITE-ProRule annotationAdd BLAST | 149 | |
Domaini | 1712 – 1775 | DRBM 1PROSITE-ProRule annotationAdd BLAST | 64 | |
Domaini | 1797 – 1872 | DRBM 2PROSITE-ProRule annotationAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 71 – 97 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 129 – 188 | DisorderedSequence analysisAdd BLAST | 60 | |
Regioni | 221 – 262 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 577 – 604 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 901 – 928 | DisorderedSequence analysisAdd BLAST | 28 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 358 – 361 | DECH boxBy similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 73 – 95 | Pro residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 583 – 604 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 906 – 928 | Basic and acidic residuesSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0701, Eukaryota |
HOGENOMi | CLU_805080_0_0_1 |
InParanoidi | Q8LMR2 |
Family and domain databases
CDDi | cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 2 hits 3.30.160.380, 1 hit 3.40.50.300, 3 hits |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR014720, dsRBD_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF02170, PAZ, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00358, DSRM, 2 hits SM00490, HELICc, 1 hit SM00949, PAZ, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS50137, DS_RBD, 2 hits PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS50821, PAZ, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8LMR2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGGGGVGGG AGEHAAAAYW YDACEDGASL LCGIDFAASA DFDPGLIPAM
60 70 80 90 100
DTGADDGFVA EIDRILESIN AESSPAPPPP PPPPLPEPVP VAPPELPIQE
110 120 130 140 150
KQLQVASAPV ANNAVAVVGV VQRSKGVVAR KEPRRESHGC AANGGGGGEW
160 170 180 190 200
RDGKRPRLAS GGVGGPRQEW RRRPMLPPPP SRGWDDRRGR RDFDRVRKHE
210 220 230 240 250
HHRREARGFW ERDRGGKMVF RSGTWEQESD REAKRARTQD GGSMEKKAEA
260 270 280 290 300
DRMGAAQREK PVAEERARQY QLEVLEQAKS RNTIAFLETG AGKTLIAVLL
310 320 330 340 350
IKSVCDKMLK ENKKMLAVFL VPKVPLVYQV LVMTAQILLN ILRHSIIKMD
360 370 380 390 400
AIHLLILDEC HHAVKKHPYS LVMSEFYHTT PKEKRPAVFG MTASPVNLKG
410 420 430 440 450
VTSQEDCAIK IRNLESKLDS VVCTIKDRKE LEKHVPMPLE VVVQYDKAAT
460 470 480 490 500
LWSLHEQIKQ MESTVEEAAL SSSKRTKWQF MGARDAGSRD ELRLVYGVSE
510 520 530 540 550
RTESDGAANL IQKLRAINYA LGELGQWCAY KVAQSFLTAL QNDERANYQV
560 570 580 590 600
DVKFQESYLK KVVDLLHCQL TEGAAMKSET SDVEMQNTEK HNTNDLEEGE
610 620 630 640 650
LPDSHGEHVD EVIGAAVADG KVTPRVQALI KILLKYQHTE DFRAIIFVER
660 670 680 690 700
VVTALVLPKV LAELPSLSFI RCASLIGHNN NQEMRACQMQ DTISKFRDGR
710 720 730 740 750
VTLLVATSVA EEGLDIRQCN VVIRFDLAKT VLAYIQSRGR ARKPGSDYIL
760 770 780 790 800
MLERGNISHE TFLRNARNSE ETLRKEAMER TDLSHLDGTS VLSPVDTSPG
810 820 830 840 850
SMYQVESTGA VVSLNSAVGL IHFYCSQLPS DRYSILHPEF IMQKYEKPGG
860 870 880 890 900
SVEYSCKLQL PCNAPFEKLE GPICSSIRLA QQAVCLAACK KLHEMGAFTD
910 920 930 940 950
TLLPDRGSGE GEKTEQNDEG EPLPGTARHR EFYPEGVADI LRGEWILSGR
960 970 980 990 1000
DGYQNSQFIK LYMYSVNCVN VGTSKDPFVT QLSNFAIIFG NELDAEVLST
1010 1020 1030 1040 1050
TMDLFVARTM ITKASLVFRG RIEITESQLV LLKSFHVRLM SIVLDVDVDP
1060 1070 1080 1090 1100
STTPWDPAKA YLFVPVGAEK CTDPLREIDW TLVNNIVNTD AWNNPLQRAR
1110 1120 1130 1140 1150
PDVYLGTNER TLGGDRREYG FGKLRHGTAF GQKAHPTYGI RGAIAEFDIV
1160 1170 1180 1190 1200
KASGLVPARD RGHFSDYQNQ GKLFMADSCW NAKDLAGMVV TAAHSGKRFY
1210 1220 1230 1240 1250
VDCICYNMNA ENSFPRKEGY LGPLEYSSYA DYYKQKYGVE LIYRKQPLIR
1260 1270 1280 1290 1300
ARGVSYCKNL LSPRFEHSDA REGDFSENLD KTYYVYLPPE LCLVHPLPGS
1310 1320 1330 1340 1350
LVRGAQRLPS IMRRVESMLL AVQLKDIIDY PVPATKILEA LTAASCQETL
1360 1370 1380 1390 1400
CYERAELLGD AYLKWVVSRF LFLKYPQKHE GQLTRMRQQM VSNMVLYQYA
1410 1420 1430 1440 1450
LNKTLQSYIQ ADRFAPSRWA APGVLPVFDE ESREYEPSIF DEESTGCELQ
1460 1470 1480 1490 1500
KESYDDYADN MQEDGEIEGD SSCYRVLSSK TLADVVEALI GVYYVAGGKI
1510 1520 1530 1540 1550
AANHLMKWIG IHAELDPEEI PPPKPYDIPE SIMRSINFDT LKGVLGIEFQ
1560 1570 1580 1590 1600
NKGLLVEAIT HASRPSSGVS CYQRLEFVGD AVLDHLITRH LFFTYTDLPP
1610 1620 1630 1640 1650
GRLTDLRAAA VNNENFARVA VKHKLHVHLR HGSSALETQI REFVKDVQEE
1660 1670 1680 1690 1700
LLKPGFNSFG LGDCKAPKVL GDIVESIAGA IFLDSGYDTS VVWKVFQPLL
1710 1720 1730 1740 1750
HPMVTPETLP MHPVRELQER CQQQAEGLEY KASRAGNIAT VEVFVDGVQI
1760 1770 1780 1790 1800
GVAQNPQKKM AQKLAARNAL VVLKEKETAT KKEDERDGEK KNGAQMFTRQ
1810 1820 1830 1840 1850
TLNDICLRRQ WPMPQYRCVN EGGPAHAKRF VYSVRVNTSD RGWTDECIGE
1860 1870 1880
PMPSVKKAKD SAAVLLLELL NRDFPDKPDG KQP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0P0VSG3 | A0A0P0VSG3_ORYSJ | Os03g0121800 protein | Os03g0121800, OSNPB_030121800 | 244 | Annotation score: |
Sequence cautioni
The sequence BAF10710 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 79 | P → T in EAZ25399 (PubMed:15685292).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC105363 Genomic DNA Translation: AAM52322.1 DP000009 Genomic DNA Translation: ABF93701.1 AP008209 Genomic DNA Translation: BAF10710.2 Sequence problems. AP014959 Genomic DNA No translation available. CM000140 Genomic DNA Translation: EAZ25399.1 |
Genome annotation databases
EnsemblPlantsi | Os03t0121800-01; Os03t0121800-01; Os03g0121800 |
Gramenei | Os03t0121800-01; Os03t0121800-01; Os03g0121800 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC105363 Genomic DNA Translation: AAM52322.1 DP000009 Genomic DNA Translation: ABF93701.1 AP008209 Genomic DNA Translation: BAF10710.2 Sequence problems. AP014959 Genomic DNA No translation available. CM000140 Genomic DNA Translation: EAZ25399.1 |
3D structure databases
AlphaFoldDBi | Q8LMR2 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 4530.OS03T0121901-00 |
Proteomic databases
PaxDbi | Q8LMR2 |
Genome annotation databases
EnsemblPlantsi | Os03t0121800-01; Os03t0121800-01; Os03g0121800 |
Gramenei | Os03t0121800-01; Os03t0121800-01; Os03g0121800 |
Phylogenomic databases
eggNOGi | KOG0701, Eukaryota |
HOGENOMi | CLU_805080_0_0_1 |
InParanoidi | Q8LMR2 |
Family and domain databases
CDDi | cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 2 hits 3.30.160.380, 1 hit 3.40.50.300, 3 hits |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR014720, dsRBD_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF02170, PAZ, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00358, DSRM, 2 hits SM00490, HELICc, 1 hit SM00949, PAZ, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS50137, DS_RBD, 2 hits PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS50821, PAZ, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | DCL1_ORYSJ | |
Accessioni | Q8LMR2Primary (citable) accession number: Q8LMR2 Secondary accession number(s): A3ADL0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 7, 2009 |
Last sequence update: | October 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - SIMILARITY comments
Index of protein domains and families