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UniProtKB - Q8LKS5 (LACS7_ARATH)
Protein
Long chain acyl-CoA synthetase 7, peroxisomal
Gene
LACS7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation (Probable). Preferentially uses palmitate, palmitoleate, oleate, linoleate and eicosenoate as substrates (PubMed:12177484, PubMed:12366803).
Can use myristate and linolenate as substrates (PubMed:12366803).
Functions redundantly with LACS6 in lipid mobilization for beta-oxidation during seed germination, which is essential for postgerminative growth and seedling establishment (PubMed:14742880, PubMed:16844736).
2 Publications4 PublicationsCatalytic activityi
- EC:6.2.1.32 PublicationsThis reaction proceeds in the forward2 Publications direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- (9Z,12Z)-octadecadienoate + ATP + CoA = (9Z,12Z)-octadecadienoyl-CoA + AMP + diphosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z,12Z,15Z)-octadecatrienoate + ATP + CoA = (9Z,12Z,15Z)-octadecatrienoyl-CoA + AMP + diphosphate1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Mg2+By similarity
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.CuratedView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 266 – 277 | ATPSequence analysisAdd BLAST | 12 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- decanoate-CoA ligase activity Source: RHEA
- long-chain fatty acid-CoA ligase activity Source: UniProtKB
GO - Biological processi
- fatty acid metabolic process Source: UniProtKB
- response to ozone Source: TAIR
- response to salt stress Source: TAIR
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT5G27600-MONOMER |
UniPathwayi | UPA00199 |
Chemistry databases
SwissLipidsi | SLP:000001940 |
Names & Taxonomyi
Protein namesi | Recommended name: Long chain acyl-CoA synthetase 7, peroxisomal1 Publication (EC:6.2.1.32 Publications)Short name: AtLACS71 Publication |
Gene namesi | Name:LACS71 Publication Ordered Locus Names:At5g27600Imported ORF Names:F15A18.60Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G27600 |
TAIRi | locus:2143661, AT5G27600 |
Subcellular locationi
Peroxisome
- Peroxisome 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Peroxisome
- peroxisome Source: UniProtKB
Other locations
Keywords - Cellular componenti
PeroxisomePathology & Biotechi
Disruption phenotypei
Seedlings of the lacs6 and lacs7 double mutant were arrested in postgerminative growth due to inability to mobilize fatty acids for beta-oxidation, a necessary process to pursue the development.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000401416 | 1 – 700 | Long chain acyl-CoA synthetase 7, peroxisomalAdd BLAST | 700 |
Proteomic databases
PaxDbi | Q8LKS5 |
PRIDEi | Q8LKS5 |
ProteomicsDBi | 237056 |
Expressioni
Tissue specificityi
Expressed in roots, stems, leaves flowers and germinating seedling (PubMed:12177484). Preferentially expressed in seeds.2 Publications
Developmental stagei
Induced during seed germination.1 Publication
Inductioni
Up-regulated by ozone and salt stress.1 Publication
Gene expression databases
ExpressionAtlasi | Q8LKS5, baseline and differential |
Genevisiblei | Q8LKS5, AT |
Interactioni
Subunit structurei
Interacts with PEX5.
1 PublicationBinary interactionsi
Q8LKS5
With | #Exp. | IntAct |
---|---|---|
PEX5 [Q9FMA3] | 4 | EBI-993851,EBI-993861 |
Protein-protein interaction databases
BioGRIDi | 18094, 2 interactors |
IntActi | Q8LKS5, 1 interactor |
STRINGi | 3702.AT5G27600.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 29 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 526 – 550 | Fatty acid-bindingSequence analysisAdd BLAST | 25 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 10 – 18 | Microbody targeting signalSequence analysis | 9 | |
Motifi | 698 – 700 | Microbody targeting signalSequence analysis | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 16 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Phylogenomic databases
eggNOGi | KOG1256, Eukaryota |
HOGENOMi | CLU_000022_45_4_1 |
InParanoidi | Q8LKS5 |
OMAi | EWIVRDS |
OrthoDBi | 630541at2759 |
PhylomeDBi | Q8LKS5 |
Family and domain databases
CDDi | cd05927, LC-FACS_euk, 1 hit |
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR045311, LC-FACS_euk |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8LKS5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEFASPEQRR LETIRSHIDT SPTNDQSSSL FLNATASSAS PFFKEDSYSV
60 70 80 90 100
VLPEKLDTGK WNVYRSKRSP TKLVSRFPDH PEIGTLHDNF VHAVETYAEN
110 120 130 140 150
KYLGTRVRSD GTIGEYSWMT YGEAASERQA IGSGLLFHGV NQGDCVGLYF
160 170 180 190 200
INRPEWLVVD HACAAYSFVS VPLYDTLGPD AVKFVVNHAN LQAIFCVPQT
210 220 230 240 250
LNILLSFLAE IPSIRLIVVV GGADEHLPSL PRGTGVTIVS YQKLLSQGRS
260 270 280 290 300
SLHPFSPPKP EDIATICYTS GTTGTPKGVV LTHGNLIANV AGSSVEAEFF
310 320 330 340 350
PSDVYISYLP LAHIYERANQ IMGVYGGVAV GFYQGDVFKL MDDFAVLRPT
360 370 380 390 400
IFCSVPRLYN RIYDGITSAV KSSGVVKKRL FEIAYNSKKQ AIINGRTPSA
410 420 430 440 450
FWDKLVFNKI KEKLGGRVRF MGSGASPLSP DVMDFLRICF GCSVREGYGM
460 470 480 490 500
TETSCVISAM DDGDNLSGHV GSPNPACEVK LVDVPEMNYT SDDQPYPRGE
510 520 530 540 550
ICVRGPIIFK GYYKDEEQTR EILDGDGWLH TGDIGLWLPG GRLKIIDRKK
560 570 580 590 600
NIFKLAQGEY IAPEKIENVY TKCRFVSQCF IHGDSFNSSL VAIVSVDPEV
610 620 630 640 650
MKDWAASEGI KYEHLGQLCN DPRVRKTVLA EMDDLGREAQ LRGFEFAKAV
660 670 680 690 700
TLVPEPFTLE NGLLTPTFKI KRPQAKAYFA EAISKMYAEI AASNPIPSKL
Sequence cautioni
The sequence BAC43213 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 657 | F → L in AAM28874 (PubMed:12177484).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF503757 mRNA Translation: AAM28874.1 AC007478 Genomic DNA No translation available. CP002688 Genomic DNA Translation: AED93704.1 AK118614 mRNA Translation: BAC43213.1 Different initiation. |
RefSeqi | NP_198112.2, NM_122642.5 |
Genome annotation databases
EnsemblPlantsi | AT5G27600.1; AT5G27600.1; AT5G27600 |
GeneIDi | 832820 |
Gramenei | AT5G27600.1; AT5G27600.1; AT5G27600 |
KEGGi | ath:AT5G27600 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF503757 mRNA Translation: AAM28874.1 AC007478 Genomic DNA No translation available. CP002688 Genomic DNA Translation: AED93704.1 AK118614 mRNA Translation: BAC43213.1 Different initiation. |
RefSeqi | NP_198112.2, NM_122642.5 |
3D structure databases
SMRi | Q8LKS5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 18094, 2 interactors |
IntActi | Q8LKS5, 1 interactor |
STRINGi | 3702.AT5G27600.1 |
Chemistry databases
SwissLipidsi | SLP:000001940 |
Proteomic databases
PaxDbi | Q8LKS5 |
PRIDEi | Q8LKS5 |
ProteomicsDBi | 237056 |
Genome annotation databases
EnsemblPlantsi | AT5G27600.1; AT5G27600.1; AT5G27600 |
GeneIDi | 832820 |
Gramenei | AT5G27600.1; AT5G27600.1; AT5G27600 |
KEGGi | ath:AT5G27600 |
Organism-specific databases
Araporti | AT5G27600 |
TAIRi | locus:2143661, AT5G27600 |
Phylogenomic databases
eggNOGi | KOG1256, Eukaryota |
HOGENOMi | CLU_000022_45_4_1 |
InParanoidi | Q8LKS5 |
OMAi | EWIVRDS |
OrthoDBi | 630541at2759 |
PhylomeDBi | Q8LKS5 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
BioCyci | ARA:AT5G27600-MONOMER |
Miscellaneous databases
PROi | PR:Q8LKS5 |
Gene expression databases
ExpressionAtlasi | Q8LKS5, baseline and differential |
Genevisiblei | Q8LKS5, AT |
Family and domain databases
CDDi | cd05927, LC-FACS_euk, 1 hit |
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR045311, LC-FACS_euk |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LACS7_ARATH | |
Accessioni | Q8LKS5Primary (citable) accession number: Q8LKS5 Secondary accession number(s): Q8GWV4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 30, 2010 |
Last sequence update: | November 30, 2010 | |
Last modified: | February 23, 2022 | |
This is version 119 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families