UniProtKB - Q8LG88 (TDT_ARATH)
Protein
Tonoplast dicarboxylate transporter
Gene
TDT
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Putative carrier protein indirectly involved in the uptake of malate and fumarate to the vacuole, probably by regulating the energization across the tonoplast. Uptake of malate to vacuoles is inhibited by citrate and by the uncoupler carbonyl-cyanide m-chlorophenylhydrazone, but seems to be not affected by sodium. Critical for pH homeostasis.2 Publications
GO - Molecular functioni
- malate transmembrane transporter activity Source: TAIR
GO - Biological processi
- malate transport Source: TAIR
- regulation of intracellular pH Source: TAIR
Keywordsi
Biological process | Transport |
Protein family/group databases
TCDBi | 2.A.47.1.6 the divalent anion:na(+) symporter (dass) family |
Names & Taxonomyi
Protein namesi | Recommended name: Tonoplast dicarboxylate transporterShort name: AttDT Alternative name(s): Sodium-dicarboxylate cotransporter-like Short name: AtSDAT Vacuolar malate transporter |
Gene namesi | Name:TDT Synonyms:SDAT Ordered Locus Names:At5g47560 ORF Names:MNJ7.15 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G47560 |
TAIRi | locus:2168988 AT5G47560 |
Subcellular locationi
Vacuole
- Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Tonoplast.
Vacuole
- vacuolar membrane Source: UniProtKB-SubCell
- vacuole Source: TAIR
Other locations
- cell Source: GOC
- integral component of membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 46 – 66 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 75 – 95 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 123 – 143 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 164 – 184 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 219 – 239 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 265 – 285 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 311 – 331 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 353 – 373 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 392 – 412 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 419 – 439 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 466 – 486 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 520 – 540 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000172526 | 1 – 540 | Tonoplast dicarboxylate transporterAdd BLAST | 540 |
Proteomic databases
PaxDbi | Q8LG88 |
Expressioni
Tissue specificityi
Expressed in leaves, stems and flowers.1 Publication
Inductioni
Induced by malate and by acidification of the cytoplasm.2 Publications
Gene expression databases
ExpressionAtlasi | Q8LG88 baseline and differential |
Genevisiblei | Q8LG88 AT |
Interactioni
Protein-protein interaction databases
BioGridi | 20054, 2 interactors |
IntActi | Q8LG88, 1 interactor |
STRINGi | 3702.AT5G47560.1 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 201 – 204 | Poly-Ser | 4 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1281 Eukaryota COG0471 LUCA |
HOGENOMi | HOG000278432 |
InParanoidi | Q8LG88 |
KOi | K14445 |
OMAi | IWWMTEA |
OrthoDBi | 389981at2759 |
PhylomeDBi | Q8LG88 |
Family and domain databases
InterProi | View protein in InterPro IPR001898 SLC13A/DASS |
Pfami | View protein in Pfam PF00939 Na_sulph_symp, 1 hit |
TIGRFAMsi | TIGR00785 dass, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8LG88-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNGGDVTVAG SDDLKSPLLP VVHNDEPFER QTVGQQLRTI FTPKNCYIAL
60 70 80 90 100
GPLLCAVVCL CVDLGGDETT TARNMLGVLV WMFAWWLTEA VPMPITSMTP
110 120 130 140 150
LFLFPLFGIS AADDVANSYM DDVISLVLGS FILALAVEHY NIHRRLALNI
160 170 180 190 200
TLVFCVEPLN APLLLLGICA TTAFVSMWMH NVAAAVMMMP VATGILQRLP
210 220 230 240 250
SSSSTTEVVH PAVGKFSRAV VLGVIYSAAV GGMSTLTGTG VNLILVGMWK
260 270 280 290 300
SYFPEADPIS FSQWFFFGFP LALCIFVVLW CVLCVMYCPK GAGQALSPYL
310 320 330 340 350
HKSHLRRELD LLGPMNFAEK MVLAVFGGLV VLWMTRNITD DIPGWGRIFA
360 370 380 390 400
GRAGDGTVSV MMATLLFIIP SNIKKGEKLM DWNKCKKLPW NIVLLLGAGF
410 420 430 440 450
AIADGVRTSG LAEVLSKGLV FLETAPYWAI APTVCLIAAT ITEFTSNNAT
460 470 480 490 500
TTLLVPLLIE IAKNMGIHPL LLMVPGAIGA QFAFLLPTGT PSNVVGFTTG
510 520 530 540
HIEIKDMIKT GLPLKIAGTI FLSILMPTLG AYVFASMGGV
Sequence cautioni
The sequence BAB09082 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAC19843 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 310 | D → E in AAM60982 (Ref. 6) Curated | 1 | |
Sequence conflicti | 407 | R → P in CAC19843 (PubMed:12947042).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223445 mRNA Translation: CAC19843.1 Frameshift. AB043024 mRNA Translation: BAA96091.1 AB025628 Genomic DNA Translation: BAB09082.1 Sequence problems. CP002688 Genomic DNA Translation: AED95532.1 AY072145 mRNA Translation: AAL59967.1 AY133792 mRNA Translation: AAM91726.1 AY084408 mRNA Translation: AAM60982.1 |
RefSeqi | NP_199567.1, NM_124129.4 |
Genome annotation databases
EnsemblPlantsi | AT5G47560.1; AT5G47560.1; AT5G47560 |
GeneIDi | 834806 |
Gramenei | AT5G47560.1; AT5G47560.1; AT5G47560 |
KEGGi | ath:AT5G47560 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223445 mRNA Translation: CAC19843.1 Frameshift. AB043024 mRNA Translation: BAA96091.1 AB025628 Genomic DNA Translation: BAB09082.1 Sequence problems. CP002688 Genomic DNA Translation: AED95532.1 AY072145 mRNA Translation: AAL59967.1 AY133792 mRNA Translation: AAM91726.1 AY084408 mRNA Translation: AAM60982.1 |
RefSeqi | NP_199567.1, NM_124129.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGridi | 20054, 2 interactors |
IntActi | Q8LG88, 1 interactor |
STRINGi | 3702.AT5G47560.1 |
Protein family/group databases
TCDBi | 2.A.47.1.6 the divalent anion:na(+) symporter (dass) family |
Proteomic databases
PaxDbi | Q8LG88 |
Genome annotation databases
EnsemblPlantsi | AT5G47560.1; AT5G47560.1; AT5G47560 |
GeneIDi | 834806 |
Gramenei | AT5G47560.1; AT5G47560.1; AT5G47560 |
KEGGi | ath:AT5G47560 |
Organism-specific databases
Araporti | AT5G47560 |
TAIRi | locus:2168988 AT5G47560 |
Phylogenomic databases
eggNOGi | KOG1281 Eukaryota COG0471 LUCA |
HOGENOMi | HOG000278432 |
InParanoidi | Q8LG88 |
KOi | K14445 |
OMAi | IWWMTEA |
OrthoDBi | 389981at2759 |
PhylomeDBi | Q8LG88 |
Miscellaneous databases
PROi | PR:Q8LG88 |
Gene expression databases
ExpressionAtlasi | Q8LG88 baseline and differential |
Genevisiblei | Q8LG88 AT |
Family and domain databases
InterProi | View protein in InterPro IPR001898 SLC13A/DASS |
Pfami | View protein in Pfam PF00939 Na_sulph_symp, 1 hit |
TIGRFAMsi | TIGR00785 dass, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TDT_ARATH | |
Accessioni | Q8LG88Primary (citable) accession number: Q8LG88 Secondary accession number(s): Q9FEH9, Q9FGK1, Q9MAW4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | November 13, 2019 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names