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UniProtKB - Q8LG50 (LPAT2_ARATH)
Protein
1-acyl-sn-glycerol-3-phosphate acyltransferase 2
Gene
LPAT2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position. Has preference for C-18-CoA substrates compared to C-16-CoA substrates. Required for female but not male gametophyte development.
1 PublicationCatalytic activityi
- a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA1 PublicationEC:2.3.1.511 Publication
: CDP-diacylglycerol biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate. This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
- 1-acylglycerol-3-phosphate O-acyltransferase activity Source: TAIR
- acyltransferase activity Source: GO_Central
GO - Biological processi
- CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Acyltransferase, Developmental protein, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Enzyme and pathway databases
BioCyci | ARA:AT3G57650-MONOMER |
BRENDAi | 2.3.1.51, 399 |
UniPathwayi | UPA00557;UER00613 |
Names & Taxonomyi
Protein namesi | Recommended name: 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 (EC:2.3.1.51)Alternative name(s): Lysophosphatidyl acyltransferase 2 |
Gene namesi | Name:LPAT2 Synonyms:LPAAT2 Ordered Locus Names:At3g57650 ORF Names:F15B8.160 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G57650 |
TAIRi | locus:2076676, AT3G57650 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- endomembrane system Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 2 – 22 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 305 – 325 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 333 – 353 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208179 | 1 – 389 | 1-acyl-sn-glycerol-3-phosphate acyltransferase 2Add BLAST | 389 |
Proteomic databases
PaxDbi | Q8LG50 |
PRIDEi | Q8LG50 |
ProteomicsDBi | 238793 |
Expressioni
Tissue specificityi
Present in roots, leaves, stems, floral buds and siliques (at protein level). Widely expressed. In contrast to LPAT1, it is not expressed at higher level in leaves.2 Publications
Gene expression databases
ExpressionAtlasi | Q8LG50, baseline and differential |
Genevisiblei | Q8LG50, AT |
Interactioni
Subunit structurei
Interacts with GPAT9 and DGAT1.
1 PublicationProtein-protein interaction databases
BioGRIDi | 10249, 17 interactors |
IntActi | Q8LG50, 17 interactors |
STRINGi | 3702.AT3G57650.1 |
Structurei
3D structure databases
AlphaFoldDBi | Q8LG50 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 357 – 389 | DisorderedSequence analysisAdd BLAST | 33 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 91 – 96 | HXXXXD motif | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 369 – 389 | Basic and acidic residuesSequence analysisAdd BLAST | 21 |
Domaini
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity
Sequence similaritiesi
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1505, Eukaryota |
HOGENOMi | CLU_041844_5_0_1 |
InParanoidi | Q8LG50 |
OMAi | WPTWLIS |
OrthoDBi | 959325at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR032098, Acyltransf_C IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF16076, Acyltransf_C, 1 hit PF01553, Acyltransferase, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8LG50-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVIAAAVIVP LGLLFFISGL AVNLFQAVCY VLIRPLSKNT YRKINRVVAE
60 70 80 90 100
TLWLELVWIV DWWAGVKIQV FADNETFNRM GKEHALVVCN HRSDIDWLVG
110 120 130 140 150
WILAQRSGCL GSALAVMKKS SKFLPVIGWS MWFSEYLFLE RNWAKDESTL
160 170 180 190 200
KSGLQRLSDF PRPFWLALFV EGTRFTEAKL KAAQEYAASS ELPIPRNVLI
210 220 230 240 250
PRTKGFVSAV SNMRSFVPAI YDMTVTIPKT SPPPTMLRLF KGQPSVVHVH
260 270 280 290 300
IKCHSMKDLP ESDDAIAQWC RDQFVAKDAL LDKHIAADTF PGQQEQNIGR
310 320 330 340 350
PIKSLAVVLS WACVLTLGAI KFLHWAQLFS SWKGITISAL GLGIITLCMQ
360 370 380
ILIRSSQSER STPAKVVPAK PKDNHHPESS SQTETEKEK
Sequence cautioni
The sequence CAB41190 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 205 | G → S in AAM61033 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL049660 Genomic DNA Translation: CAB41190.1 Sequence problems. CP002686 Genomic DNA Translation: AEE79683.1 AY084461 mRNA Translation: AAM61033.1 |
PIRi | T06755 |
RefSeqi | NP_567052.1, NM_115625.4 |
Genome annotation databases
EnsemblPlantsi | AT3G57650.1; AT3G57650.1; AT3G57650 |
GeneIDi | 824934 |
Gramenei | AT3G57650.1; AT3G57650.1; AT3G57650 |
KEGGi | ath:AT3G57650 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL049660 Genomic DNA Translation: CAB41190.1 Sequence problems. CP002686 Genomic DNA Translation: AEE79683.1 AY084461 mRNA Translation: AAM61033.1 |
PIRi | T06755 |
RefSeqi | NP_567052.1, NM_115625.4 |
3D structure databases
AlphaFoldDBi | Q8LG50 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 10249, 17 interactors |
IntActi | Q8LG50, 17 interactors |
STRINGi | 3702.AT3G57650.1 |
Proteomic databases
PaxDbi | Q8LG50 |
PRIDEi | Q8LG50 |
ProteomicsDBi | 238793 |
Genome annotation databases
EnsemblPlantsi | AT3G57650.1; AT3G57650.1; AT3G57650 |
GeneIDi | 824934 |
Gramenei | AT3G57650.1; AT3G57650.1; AT3G57650 |
KEGGi | ath:AT3G57650 |
Organism-specific databases
Araporti | AT3G57650 |
TAIRi | locus:2076676, AT3G57650 |
Phylogenomic databases
eggNOGi | KOG1505, Eukaryota |
HOGENOMi | CLU_041844_5_0_1 |
InParanoidi | Q8LG50 |
OMAi | WPTWLIS |
OrthoDBi | 959325at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00557;UER00613 |
BioCyci | ARA:AT3G57650-MONOMER |
BRENDAi | 2.3.1.51, 399 |
Miscellaneous databases
PROi | PR:Q8LG50 |
Gene expression databases
ExpressionAtlasi | Q8LG50, baseline and differential |
Genevisiblei | Q8LG50, AT |
Family and domain databases
InterProi | View protein in InterPro IPR032098, Acyltransf_C IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF16076, Acyltransf_C, 1 hit PF01553, Acyltransferase, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LPAT2_ARATH | |
Accessioni | Q8LG50Primary (citable) accession number: Q8LG50 Secondary accession number(s): Q9SVX9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | May 3, 2011 | |
Last modified: | May 25, 2022 | |
This is version 119 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families