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UniProtKB - Q8LBA6 (PIS1_ARATH)
Protein
CDP-diacylglycerol--inositol 3-phosphatidyltransferase 1
Gene
PIS1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme.
4 PublicationsCatalytic activityi
- a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H+1 PublicationEC:2.7.8.111 Publication
Cofactori
Kineticsi
- KM=400 µM for myo-inositol1 Publication
- KM=10 µM for CDP-diacylglycerol1 Publication
GO - Molecular functioni
- CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- phosphatidylinositol biosynthetic process Source: GO_Central
- phospholipid biosynthetic process Source: TAIR
Keywordsi
Molecular function | Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Ligand | Magnesium, Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.7.8.11, 399 |
SABIO-RKi | Q8LBA6 |
Names & Taxonomyi
Protein namesi | Recommended name: CDP-diacylglycerol--inositol 3-phosphatidyltransferase 1 (EC:2.7.8.11)Alternative name(s): Phosphatidylinositol synthase 1 Short name: AtPIS1 Short name: PI synthase 1 Short name: PtdIns synthase 1 |
Gene namesi | Name:PIS1 Ordered Locus Names:At1g68000 ORF Names:T23K23.15 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G68000 |
TAIRi | locus:2200241, AT1G68000 |
Subcellular locationi
Other locations
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
Golgi apparatus
- Golgi apparatus Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: TAIR
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 13 – 35 | HelicalSequence analysisAdd BLAST | 23 | |
Transmembranei | 43 – 62 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 87 – 107 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 146 – 166 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 176 – 196 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056805 | 1 – 227 | CDP-diacylglycerol--inositol 3-phosphatidyltransferase 1Add BLAST | 227 |
Proteomic databases
PaxDbi | Q8LBA6 |
ProteomicsDBi | 235089 |
Expressioni
Tissue specificityi
Expressed in stems, flowers, shoots and roots. Present in epidermal tissues.1 Publication
Gene expression databases
ExpressionAtlasi | Q8LBA6, baseline and differential |
Genevisiblei | Q8LBA6, AT |
Family & Domainsi
Sequence similaritiesi
Belongs to the CDP-alcohol phosphatidyltransferase class-I family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3240, Eukaryota |
HOGENOMi | CLU_067602_2_1_1 |
InParanoidi | Q8LBA6 |
OMAi | QEFAYIF |
OrthoDBi | 1615564at2759 |
PhylomeDBi | Q8LBA6 |
Family and domain databases
Gene3Di | 1.20.120.1760, 1 hit |
InterProi | View protein in InterPro IPR000462, CDP-OH_P_trans IPR043130, CDP-OH_PTrfase_TM_dom IPR014387, CDP_diag_ino_3_P_euk |
Pfami | View protein in Pfam PF01066, CDP-OH_P_transf, 1 hit |
PIRSFi | PIRSF000848, CDP_diag_ino_3_P, 1 hit |
PROSITEi | View protein in PROSITE PS00379, CDP_ALCOHOL_P_TRANSF, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8LBA6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKKERPRPE KLSVYLYIPN IVGYMRVLLN CVAFAVCFSN KPLFSVLYFF
60 70 80 90 100
SFCCDAVDGW VARRFNQVST FGAVLDMVTD RVSTACLLVI LSQIYRPSLV
110 120 130 140 150
FLSLLALDIA SHWLQMYSTF LAGKSSHKDV KDSTSWLFRL YYGNRIFMCY
160 170 180 190 200
CCVSCEVLYI ILLLIAKNQS ENLLNVVVAT LTQISPLSFL LALTLFGWSM
210 220
KQTINVIQMK TAADVCVLYD IEKQQKP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 221 | I → L in AAM64877 (Ref. 6) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ000539 mRNA Translation: CAA04172.1 AC012563 Genomic DNA Translation: AAG52009.1 CP002684 Genomic DNA Translation: AEE34734.1 AY140047 mRNA Translation: AAM98188.1 AY087327 mRNA Translation: AAM64877.1 |
PIRi | T52643 |
RefSeqi | NP_176967.1, NM_105470.3 |
Genome annotation databases
EnsemblPlantsi | AT1G68000.1; AT1G68000.1; AT1G68000 |
GeneIDi | 843128 |
Gramenei | AT1G68000.1; AT1G68000.1; AT1G68000 |
KEGGi | ath:AT1G68000 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ000539 mRNA Translation: CAA04172.1 AC012563 Genomic DNA Translation: AAG52009.1 CP002684 Genomic DNA Translation: AEE34734.1 AY140047 mRNA Translation: AAM98188.1 AY087327 mRNA Translation: AAM64877.1 |
PIRi | T52643 |
RefSeqi | NP_176967.1, NM_105470.3 |
3D structure databases
AlphaFoldDBi | Q8LBA6 |
SMRi | Q8LBA6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G68000.1 |
Proteomic databases
PaxDbi | Q8LBA6 |
ProteomicsDBi | 235089 |
Genome annotation databases
EnsemblPlantsi | AT1G68000.1; AT1G68000.1; AT1G68000 |
GeneIDi | 843128 |
Gramenei | AT1G68000.1; AT1G68000.1; AT1G68000 |
KEGGi | ath:AT1G68000 |
Organism-specific databases
Araporti | AT1G68000 |
TAIRi | locus:2200241, AT1G68000 |
Phylogenomic databases
eggNOGi | KOG3240, Eukaryota |
HOGENOMi | CLU_067602_2_1_1 |
InParanoidi | Q8LBA6 |
OMAi | QEFAYIF |
OrthoDBi | 1615564at2759 |
PhylomeDBi | Q8LBA6 |
Enzyme and pathway databases
BRENDAi | 2.7.8.11, 399 |
SABIO-RKi | Q8LBA6 |
Miscellaneous databases
PROi | PR:Q8LBA6 |
Gene expression databases
ExpressionAtlasi | Q8LBA6, baseline and differential |
Genevisiblei | Q8LBA6, AT |
Family and domain databases
Gene3Di | 1.20.120.1760, 1 hit |
InterProi | View protein in InterPro IPR000462, CDP-OH_P_trans IPR043130, CDP-OH_PTrfase_TM_dom IPR014387, CDP_diag_ino_3_P_euk |
Pfami | View protein in Pfam PF01066, CDP-OH_P_transf, 1 hit |
PIRSFi | PIRSF000848, CDP_diag_ino_3_P, 1 hit |
PROSITEi | View protein in PROSITE PS00379, CDP_ALCOHOL_P_TRANSF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PIS1_ARATH | |
Accessioni | Q8LBA6Primary (citable) accession number: Q8LBA6 Secondary accession number(s): O81846 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | May 25, 2022 | |
This is version 114 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families